##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139613_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7242913
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.225235896109755	18.0	18.0	28.0	18.0	33.0
2	29.808822223875946	31.0	27.0	32.0	27.0	33.0
3	30.848427973661977	31.0	30.0	33.0	27.0	33.0
4	32.129450954332874	33.0	33.0	33.0	30.0	33.0
5	32.50386771178944	33.0	33.0	33.0	32.0	34.0
6	36.226862589679044	38.0	36.0	38.0	33.0	38.0
7	36.39628972486622	38.0	37.0	38.0	34.0	38.0
8	36.01111735016008	38.0	36.0	38.0	33.0	38.0
9	36.897653471745414	38.0	38.0	38.0	35.0	38.0
10-11	37.02850434624853	38.0	38.0	38.0	35.0	38.0
12-13	37.1579441172357	38.0	38.0	38.0	36.0	38.0
14-15	37.04324068230558	38.0	38.0	38.0	35.5	38.0
16-17	37.138294840763656	38.0	38.0	38.0	36.0	38.0
18-19	37.26254547031009	38.0	38.0	38.0	36.0	38.0
20-21	37.32529308530209	38.0	38.0	38.0	37.0	38.0
22-23	37.37379630623801	38.0	38.0	38.0	37.0	38.0
24-25	37.39606828372193	38.0	38.0	38.0	37.0	38.0
26-27	37.37507287962041	38.0	38.0	38.0	37.0	38.0
28-29	37.3504870341103	38.0	38.0	38.0	37.0	38.0
30-31	37.36033820052518	38.0	38.0	38.0	37.0	38.0
32-33	37.35124740083826	38.0	38.0	38.0	37.0	38.0
34-35	37.3253475080531	38.0	38.0	38.0	37.0	38.0
36-37	37.303750298181114	38.0	38.0	38.0	37.0	38.0
38-39	37.28054742281075	38.0	38.0	38.0	37.0	38.0
40-41	37.290518397735326	38.0	38.0	38.0	37.0	38.0
42-43	37.26462451221339	38.0	38.0	38.0	37.0	38.0
44-45	37.226804121291416	38.0	38.0	38.0	37.0	38.0
46-47	37.193705335217544	38.0	38.0	38.0	37.0	38.0
48-49	37.14042670279319	38.0	38.0	38.0	36.5	38.0
50-51	37.08479112251685	38.0	38.0	38.0	36.0	38.0
52-53	37.058259030419535	38.0	38.0	38.0	36.0	38.0
54-55	37.02719607826724	38.0	38.0	38.0	36.0	38.0
56-57	36.96337570083118	38.0	38.0	38.0	36.0	38.0
58-59	36.93154636879656	38.0	38.0	38.0	36.0	38.0
60-61	36.94967443283245	38.0	38.0	38.0	36.0	38.0
62-63	36.95079956030632	38.0	38.0	38.0	36.0	38.0
64-65	36.951475361672706	38.0	38.0	38.0	36.0	38.0
66-67	36.94462133559979	38.0	38.0	38.0	36.0	38.0
68-69	36.92833568034951	38.0	38.0	38.0	36.0	38.0
70-71	36.917596814661934	38.0	38.0	38.0	35.0	38.0
72-73	36.91048152652807	38.0	38.0	38.0	35.0	38.0
74-75	36.88361822717632	38.0	38.0	38.0	35.0	38.0
76-77	36.85599693867232	38.0	38.0	38.0	35.0	38.0
78-79	36.85467184000083	38.0	38.0	38.0	35.0	38.0
80-81	36.848321467183965	38.0	38.0	38.0	35.0	38.0
82-83	36.788828340819066	38.0	38.0	38.0	35.0	38.0
84-85	36.763771913131336	38.0	38.0	38.0	35.0	38.0
86-87	36.7613898487738	38.0	38.0	38.0	35.0	38.0
88-89	36.73822811749218	38.0	38.0	38.0	35.0	38.0
90-91	36.70501661159053	38.0	38.0	38.0	34.0	38.0
92-93	36.69037346928018	38.0	38.0	38.0	34.0	38.0
94-95	36.65962595197865	38.0	38.0	38.0	34.0	38.0
96-97	36.620436009826236	38.0	38.0	38.0	34.0	38.0
98-99	36.597405900878556	38.0	38.0	38.0	34.0	38.0
100	35.07818381593939	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	17.0
20	42.0
21	138.0
22	449.0
23	1360.0
24	3468.0
25	6744.0
26	12448.0
27	20532.0
28	32825.0
29	48069.0
30	65920.0
31	86258.0
32	113979.0
33	158873.0
34	246979.0
35	465139.0
36	1463742.0
37	4515924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09534561577641	17.659234620103817	18.652854176213353	23.592565587906414
2	14.558976991532644	21.00179520224537	41.698673880980934	22.740553925241052
3	18.354544681349296	25.363940671554	31.152998399483945	25.128516247612758
4	12.571806398889507	17.887278226315846	38.60794683023253	30.93296854456211
5	13.494836537408785	37.019190212333136	35.690898314227525	13.795074936030554
6	30.10822463605038	37.60870303571858	18.691648011610823	13.591424316620227
7	26.66040583395106	32.45753469633005	22.838145370515978	18.043914099202905
8	24.269779300124135	36.784605862309824	21.125699010881398	17.81991582668465
9	25.425999180164116	16.974082113094553	21.299634553114196	36.30028415362714
10-11	24.131858273045665	26.658051808712873	28.92441204250279	20.28567787573867
12-13	25.029722157369555	24.22311989664932	29.014389652340157	21.73276829364097
14-15	22.151301555051123	25.644944237215057	26.743583693466977	25.460170514266846
16-17	21.741666647107316	28.81578861985502	27.420341787896664	22.022202945140997
18-19	21.68579824167431	27.81980813520748	29.25741341915884	21.236980203959373
20-21	22.498886482519087	26.91822543006075	29.20984854781581	21.373039539604356
22-23	22.18562356465371	26.957858077584383	29.05796408566392	21.798554272097988
24-25	22.01991193686763	27.04027549193484	29.243697281722415	21.696115289475117
26-27	21.982507171863425	27.23547245358107	29.10735228557744	21.674668088978063
28-29	21.782755665834166	27.14262079865492	29.188092535021497	21.886531000489416
30-31	22.17663192084673	27.060831341619142	29.166487487287483	21.596049250246647
32-33	22.034274338784126	27.123440816122972	29.15599042462998	21.68629442046292
34-35	21.91059567078176	27.121439916460726	29.322109305597827	21.64585510715969
36-37	22.05638497411753	27.0871364355124	29.126286874990242	21.730191715379828
38-39	21.79016851923124	27.147138840292683	29.29424439951383	21.768448240962247
40-41	22.02195322782471	26.98227544228236	29.246422290258558	21.749349039634374
42-43	21.98125627496204	27.036561728114535	29.083097105376538	21.89908489154689
44-45	21.926318987567424	27.300414047889117	28.934872625316725	21.838394339226735
46-47	22.149968719149104	27.13225010706083	28.572725187009784	22.145055986780285
48-49	22.082159372178616	27.19084018602621	28.607904929688743	22.11909551210643
50-51	22.02158403925191	27.352180349997752	28.580735697963462	22.045499912786877
52-53	22.112752929265977	27.354815833991335	28.563874823589174	21.96855641315351
54-55	22.077613697149953	27.362280489487812	28.418876645107964	22.141229168254267
56-57	22.190596593791287	27.525922054701645	28.248568704254783	22.034912647252284
58-59	21.910972597445742	27.50503273463034	28.505347148659656	22.078647519264265
60-61	22.242161546444876	27.614282144513375	28.381081159992917	21.76247514904883
62-63	21.850953732198736	27.784061159517965	28.405228766168	21.9597563421153
64-65	21.84011379484855	27.641593124794294	28.463992463042786	22.054300617314375
66-67	21.88728047208101	27.662667240268064	28.5314802186954	21.918572068955527
68-69	21.907799702976668	27.799968934828133	28.46182705700931	21.830404305185887
70-71	21.93786308251421	27.38677490003882	28.31161524337751	22.363746774069465
72-73	22.040588621896585	27.322072838460404	28.336337901725273	22.301000637917735
74-75	21.992640840452886	27.516062382742067	28.457779836649884	22.033516940155167
76-77	22.10568211582297	27.211769619824587	28.256334856374128	22.426213407978317
78-79	22.0741999056484	27.240434355210663	27.992638687285563	22.69272705185537
80-81	22.280427500131804	27.520844020697155	27.97621149724632	22.222516981924723
82-83	22.389610297341026	27.269290238906223	28.013173543196107	22.327925920556645
84-85	22.222618909334408	27.31580242020895	27.94247220722783	22.519106463228812
86-87	22.109678992837644	27.5596031126451	27.981997531079134	22.348720363438126
88-89	22.425972827110918	27.541145000571504	27.90557345627944	22.127308716038137
90-91	22.483243635446748	27.594546450780477	27.84027266864474	22.081937245128035
92-93	22.266489064351415	27.360653062971252	27.87390770564021	22.49895016703712
94-95	22.385098104512103	27.6104235553655	27.9100890012598	22.0943893388626
96-97	22.700244398363832	27.479809174930924	27.994760011074014	21.82518641563123
98-99	23.39690478042127	29.550872113311915	28.314329698115394	18.737893408151425
100	29.75963563115942	40.6072644881884	29.633099880652182	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1737.0
1	1631.5
2	1815.5
3	2289.5
4	2899.0
5	3869.0
6	4797.5
7	6831.0
8	8829.0
9	9362.0
10	9257.5
11	9185.0
12	10142.0
13	12683.0
14	17966.0
15	24296.5
16	28569.5
17	30148.0
18	29213.5
19	26680.5
20	23928.5
21	21656.0
22	21667.0
23	25106.5
24	31470.0
25	40598.5
26	53293.5
27	67705.5
28	82198.5
29	99085.5
30	116065.0
31	131249.0
32	148866.0
33	169500.0
34	187989.0
35	205889.0
36	225871.0
37	241327.0
38	252848.0
39	263477.5
40	274997.5
41	284108.5
42	289106.5
43	296577.5
44	303779.0
45	308321.5
46	318538.5
47	325744.0
48	322219.0
49	316208.0
50	306828.0
51	292824.0
52	275737.0
53	258142.0
54	238898.0
55	218705.0
56	197546.0
57	175439.0
58	154231.0
59	131587.5
60	108764.0
61	87361.0
62	68291.5
63	52281.0
64	38870.0
65	28545.0
66	20856.5
67	15669.5
68	11759.5
69	8606.0
70	6369.5
71	4520.5
72	3147.0
73	2145.0
74	1397.5
75	899.5
76	586.5
77	365.5
78	223.5
79	139.5
80	96.0
81	64.5
82	47.5
83	31.5
84	18.0
85	10.0
86	7.5
87	5.5
88	3.0
89	1.5
90	1.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0037968149003032347
3	0.0034378433097291107
4	0.0
5	2.7613199274932616E-5
6	2.070989945619946E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.86419028549729E-4
26-27	0.0017193946543122521
28-29	0.006030114099705347
30-31	0.002074059120363873
32-33	0.0044189931372621005
34-35	0.0023123034542063925
36-37	0.0010942220683961277
38-39	4.20392305895939E-5
40-41	9.161499404819667E-5
42-43	0.0018929546214112027
44-45	4.570832404287841E-4
46-47	3.097460960826627E-4
48-49	0.0014675434245736078
50-51	4.3203626527055976E-4
52-53	0.0
54-55	0.004367106065865763
56-57	0.045098272098546235
58-59	0.06806662908115473
60-61	0.0306289046641226
62-63	0.013091921962968519
64-65	0.0014346793942783596
66-67	0.0
68-69	0.002061526656483879
70-71	0.0037549419849530244
72-73	0.012563711368598377
74-75	0.014962927986017496
76-77	0.02003994875674453
78-79	0.005655476331044922
80-81	0.007096045477618253
82-83	0.03120882755765685
84-85	0.01782211987241514
86-87	0.012835567495547016
88-89	0.0059544442297861004
90-91	3.526494420336447E-5
92-93	0.0
94-95	0.0
96-97	9.16722961095836E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	50.0
20-21	321.0
22-23	683.0
24-25	667.0
26-27	1686.0
28-29	4355.0
30-31	12549.0
32-33	16839.0
34-35	23397.0
36-37	36046.0
38-39	41113.0
40-41	41524.0
42-43	48438.0
44-45	58749.0
46-47	60000.0
48-49	55483.0
50-51	49508.0
52-53	47041.0
54-55	50555.0
56-57	53584.0
58-59	30497.0
60-61	57670.0
62-63	55362.0
64-65	60658.0
66-67	65056.0
68-69	66757.0
70-71	73038.0
72-73	58635.0
74-75	57373.0
76-77	60977.0
78-79	63361.0
80-81	62672.0
82-83	58730.0
84-85	58253.0
86-87	60485.0
88-89	63151.0
90-91	64014.0
92-93	63355.0
94-95	73281.0
96-97	250594.0
98-99	1688792.0
100-101	3547614.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.74767447394973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.74718084515659	78.7210690338429
2	6.553417139015913	11.369873933347106
3	1.5773932103675479	4.105055781911477
4	0.5293464135817713	1.83678281477377
5	0.2386381840612925	1.0350653754001748
6	0.11834455530069513	0.6159668975393425
7	0.06994724069854842	0.42474323265280806
8	0.04399071282674038	0.30528736289368635
9	0.029696641169572043	0.23185031050329566
>10	0.09046940421951796	1.1703989512952997
>50	0.0011570081725926359	0.06605467858016671
>100	3.234584694524101E-4	0.05600491096467141
>500	7.139031408333527E-5	0.03926323414528432
>1k	2.3796645645380547E-5	0.022583482149990407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	4.141979891239892E-5	0.0	1.3806599637466306E-5	0.0
5	0.0	6.903299818733154E-5	0.0	1.3806599637466306E-5	0.0
6	0.0	6.903299818733154E-5	0.0	1.3806599637466306E-5	1.3806599637466306E-5
7	0.0	6.903299818733154E-5	0.0	1.3806599637466306E-5	1.3806599637466306E-5
8	0.0	6.903299818733154E-5	0.0	1.3806599637466306E-5	1.3806599637466306E-5
9	0.0	1.3806599637466307E-4	0.0	2.7613199274932613E-5	1.3806599637466306E-5
10-11	0.0	1.3806599637466307E-4	0.0	2.7613199274932613E-5	1.3806599637466306E-5
12-13	0.0	1.3806599637466307E-4	0.0	2.7613199274932613E-5	1.3806599637466306E-5
14-15	0.0	2.070989945619946E-4	0.0	3.451649909366577E-5	2.7613199274932613E-5
16-17	0.0	2.070989945619946E-4	0.0	5.522639854986523E-5	6.212969836859838E-5
18-19	0.0	2.209055941994609E-4	0.0	8.283959782479784E-5	7.593629800606469E-5
20-21	0.0	2.4851879347439354E-4	0.0	8.283959782479784E-5	8.283959782479784E-5
22-23	0.0	2.830352925680593E-4	0.0	8.9742897643531E-5	8.283959782479784E-5
24-25	0.0	3.106484918429919E-4	0.0	9.664619746226415E-5	8.283959782479784E-5
26-27	0.0	3.244550914804582E-4	0.0	1.2425939673719677E-4	8.283959782479784E-5
28-29	0.0	3.4516499093665767E-4	0.0	3.865847898490566E-4	8.9742897643531E-5
30-31	0.0	3.727781902115903E-4	0.0	0.0013254335651967655	1.035494972809973E-4
32-33	0.0	3.7968149003032343E-4	0.0	0.0033342938124481135	1.1045279709973045E-4
34-35	0.0	4.48714488217655E-4	0.0	0.006413165531603099	1.3806599637466307E-4
36-37	0.0	5.453606856799191E-4	0.0	0.011197152305985175	1.3806599637466307E-4
38-39	0.0	6.28200283504717E-4	0.0	0.0244031648592217	1.6567919564959568E-4
40-41	0.0	7.041365815107816E-4	0.0	0.03881725488073652	1.7258249546832884E-4
42-43	0.0	7.455563804231806E-4	0.0	0.04703908496484771	1.8638909510579514E-4
44-45	0.0	8.698157771603773E-4	0.0	0.05747687429077224	1.932923949245283E-4
46-47	0.0	9.664619746226414E-4	0.0	0.0692331938820748	2.0019569474326145E-4
48-49	0.0	9.87171874078841E-4	0.0	0.07975382280582413	2.209055941994609E-4
50-51	0.0	0.0011045279709973046	0.0	0.0915998852947702	2.209055941994609E-4
52-53	0.0	0.0011045279709973046	0.0	0.1260956744889798	2.3471219383692723E-4
54-55	0.0	0.0011390444700909704	0.0	0.1712432553034946	2.554220932931267E-4
56-57	0.0	0.0011873675688221024	0.0	0.22715998383523314	2.6232539311185984E-4
58-59	0.0	0.0012356906675532345	0.0	0.43698578182562736	2.6232539311185984E-4
60-61	0.0	0.0012840137662843666	0.0	0.6245346313009696	2.6232539311185984E-4
62-63	0.0	0.0013116269655592992	0.0	0.6315414806169838	2.6232539311185984E-4
64-65	0.0	0.001435886362296496	0.0	0.6379684527482243	2.6232539311185984E-4
66-67	0.0	0.001484209461027628	0.0	0.6441814225850842	2.69228692930593E-4
68-69	0.0	0.0015049193604838274	0.0	0.6497178690397082	2.830352925680593E-4
70-71	0.0	0.0015187259601212937	0.0	0.6545777921120963	3.0374519202425876E-4
72-73	0.0	0.00153253255975876	0.0	0.6587197720033362	3.4516499093665767E-4
74-75	0.0	0.001573952358671159	0.0	0.6617848371228537	3.4516499093665767E-4
76-77	0.0	0.0016015655579460916	0.0	0.665167454034033	3.4516499093665767E-4
78-79	0.0	0.0016153721575835579	0.0	0.6673558000765714	3.4516499093665767E-4
80-81	0.0	0.001622275457402291	0.0	0.6681496795557258	3.58971590574124E-4
82-83	0.0	0.0016360820570397573	0.0	0.6687502666399555	3.727781902115903E-4
84-85	0.0	0.0016429853568584905	0.0	0.6689780755339737	3.727781902115903E-4
86-87	0.0	0.0016429853568584905	0.0	0.6691092382305296	3.727781902115903E-4
88	0.0	0.001670598556133423	0.0	0.669123044830167	3.865847898490566E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9635	0.0	49.996243	1
GTATCAA	17380	0.0	35.6777	1
ATCAACG	21505	0.0	29.606783	3
TCAACGC	21540	0.0	29.575844	4
CAACGCA	22095	0.0	28.81361	5
TATCAAC	22395	0.0	28.603567	2
AACGCAG	22845	0.0	27.848778	6
ACGCAGA	26000	0.0	24.42	7
CGCAGAG	26750	0.0	23.783213	8
TGGTATC	4675	0.0	22.562922	2
GTGGTAT	5120	0.0	21.602787	1
GCAGAGT	31255	0.0	20.505445	9
GAGTACT	17890	0.0	19.021994	12-13
CAGAGTA	30695	0.0	18.549553	10-11
AGAGTAC	28510	0.0	18.263863	10-11
GTACATG	23125	0.0	17.839201	1
TACATGG	23010	0.0	17.817001	2
ACATGGG	22915	0.0	17.237524	3
GTACTTT	19945	0.0	17.062387	14-15
AGTACTT	19580	0.0	16.63872	12-13
>>END_MODULE