FastQCFastQC Report
Fri 27 May 2016
SRR2139612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6372225
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88450.041.6006131
ATCTCGT111450.038.4778394
GTATCAA149600.033.5530431
TATCTCG131100.032.9145794
TCAACGC182150.028.302024
ATCAACG182150.028.2795543
CAACGCA184500.027.8262625
AACGCAG190100.027.0513026
TATCAAC194800.026.5537642
ACGCAGA216850.023.714337
CGCAGAG220550.023.3357778
GCAGAGT254700.020.3071339
CTATCTC153800.019.85281692-93
GTACATG176300.019.6164171
GAGTACT153550.019.47374712-13
TACATGG180300.019.3935622
ACATGGG175900.019.224673
GCCGTCT83750.018.98332890-91
AGAGTAC225950.018.90015410-11
CCGTCTT84550.018.63430890-91
Produced by FastQC (version 0.11.5)