##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6372225
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.84108815994413	18.0	18.0	27.0	18.0	33.0
2	29.616006183083616	30.0	27.0	32.0	27.0	33.0
3	30.738477690288715	31.0	29.0	33.0	27.0	33.0
4	32.11615095198302	33.0	33.0	33.0	30.0	33.0
5	32.482457854203204	33.0	33.0	33.0	32.0	34.0
6	36.1583839239826	38.0	36.0	38.0	33.0	38.0
7	36.29849134329061	38.0	37.0	38.0	33.0	38.0
8	35.888343710399425	38.0	36.0	38.0	31.0	38.0
9	36.8480560557733	38.0	38.0	38.0	35.0	38.0
10-11	36.99707959150846	38.0	38.0	38.0	35.0	38.0
12-13	37.134064082796826	38.0	38.0	38.0	36.0	38.0
14-15	37.004766153109784	38.0	38.0	38.0	35.5	38.0
16-17	37.102610312724366	38.0	38.0	38.0	36.0	38.0
18-19	37.252104484697256	38.0	38.0	38.0	36.0	38.0
20-21	37.32208317864077	38.0	38.0	38.0	37.0	38.0
22-23	37.3796999256966	38.0	38.0	38.0	37.0	38.0
24-25	37.40581635200569	38.0	38.0	38.0	37.0	38.0
26-27	37.38768748529506	38.0	38.0	38.0	37.0	38.0
28-29	37.36498971185809	38.0	38.0	38.0	37.0	38.0
30-31	37.37601307078259	38.0	38.0	38.0	37.0	38.0
32-33	37.36206833747774	38.0	38.0	38.0	37.0	38.0
34-35	37.330988368680096	38.0	38.0	38.0	37.0	38.0
36-37	37.31042327514451	38.0	38.0	38.0	37.0	38.0
38-39	37.28241870093894	38.0	38.0	38.0	37.0	38.0
40-41	37.29101828048327	38.0	38.0	38.0	37.0	38.0
42-43	37.26577072806927	38.0	38.0	38.0	37.0	38.0
44-45	37.229612602167634	38.0	38.0	38.0	37.0	38.0
46-47	37.198298668579014	38.0	38.0	38.0	37.0	38.0
48-49	37.14682069263473	38.0	38.0	38.0	36.5	38.0
50-51	37.08941100410987	38.0	38.0	38.0	36.0	38.0
52-53	37.06040896637755	38.0	38.0	38.0	36.0	38.0
54-55	37.027879246684066	38.0	38.0	38.0	36.0	38.0
56-57	36.96297262182117	38.0	38.0	38.0	36.0	38.0
58-59	36.93369255244929	38.0	38.0	38.0	36.0	38.0
60-61	36.94543212977604	38.0	38.0	38.0	36.0	38.0
62-63	36.946567070112486	38.0	38.0	38.0	36.0	38.0
64-65	36.94730988220344	38.0	38.0	38.0	36.0	38.0
66-67	36.93845897027382	38.0	38.0	38.0	36.0	38.0
68-69	36.91525835799083	38.0	38.0	38.0	35.5	38.0
70-71	36.90509032413769	38.0	38.0	38.0	35.0	38.0
72-73	36.89705104553525	38.0	38.0	38.0	35.0	38.0
74-75	36.86214091840981	38.0	38.0	38.0	35.0	38.0
76-77	36.830763004612045	38.0	38.0	38.0	35.0	38.0
78-79	36.82473339746193	38.0	38.0	38.0	35.0	38.0
80-81	36.812837901448795	38.0	38.0	38.0	35.0	38.0
82-83	36.72516514695869	38.0	38.0	38.0	34.5	38.0
84-85	36.733108891830284	38.0	38.0	38.0	35.0	38.0
86-87	36.737008675133595	38.0	38.0	38.0	35.0	38.0
88-89	36.713978562939076	38.0	38.0	38.0	34.0	38.0
90-91	36.689340453014765	38.0	38.0	38.0	34.0	38.0
92-93	36.67502056387184	38.0	38.0	38.0	34.0	38.0
94-95	36.60974159654711	38.0	38.0	38.0	34.0	38.0
96-97	36.50300884049209	38.0	38.0	38.0	34.0	38.0
98-99	36.50464996467092	38.0	37.5	38.0	34.0	38.0
100	34.997713521404464	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	15.0
20	26.0
21	104.0
22	429.0
23	1191.0
24	2959.0
25	5966.0
26	10675.0
27	18351.0
28	29148.0
29	42197.0
30	56586.0
31	74431.0
32	99013.0
33	139761.0
34	220898.0
35	427545.0
36	1389468.0
37	3853457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.420814550647535	17.714660106948514	19.162207863030574	23.702317479373374
2	14.408246493352369	21.29453234941978	41.85710884657474	22.440112310653113
3	18.184192201923192	25.662181475946277	31.42656622944702	24.727060092683516
4	12.366606640537645	18.129883988716657	38.93461075213132	30.568898618614377
5	13.165703711248023	37.23190478424876	36.143399250151795	13.458992254351426
6	29.704652103648748	38.14762027766174	18.78413060201682	13.3635970166727
7	26.262804593372017	32.60525169779787	23.21407357712573	17.91787013170439
8	23.806613859366234	37.27492359419198	21.467321069171284	17.4511414772705
9	25.223293276681225	16.852935983898874	21.615981858769896	36.30778888065001
10-11	23.81220060496922	26.880877558466626	29.345989823021	19.96093201354315
12-13	24.66497965781183	24.40982545343267	29.42232108878767	21.50287379996783
14-15	21.79179172110213	25.93474022025274	26.953834806523624	25.31963325212151
16-17	21.386799744202378	29.14901780775161	27.727285210424927	21.736897237621083
18-19	21.427240877401534	28.141151638556387	29.499907803004444	20.931699681037628
20-21	22.05729203448162	27.289696159344896	29.448014701612813	21.204997104560668
22-23	21.787894322964068	27.36411723314191	29.309368391918856	21.53862005197517
24-25	21.706578543715814	27.463552792348132	29.422885237137674	21.406983426798377
26-27	21.66553062187542	27.586563630386806	29.406006605281526	21.34189914245625
28-29	21.53266241678858	27.407857614738383	29.57605094324864	21.483429025224396
30-31	21.59758492826127	27.280093713943472	29.59674725294083	21.525574104854428
32-33	21.351880062423977	27.649923153139554	29.40096361845782	21.59723316597865
34-35	21.71320384124874	27.463026729950634	29.431931148121414	21.391838280679206
36-37	21.57016808331556	27.484477204375494	29.532864495031596	21.412490217277345
38-39	21.38618581741658	27.436237500671613	29.5386557837562	21.6389208981556
40-41	21.71750387944948	27.250906628431654	29.63942916292044	21.392160329198422
42-43	21.622377520127305	27.26641479270762	29.41211448215694	21.699093205008126
44-45	21.447663694244117	27.55850767945066	29.323298838013244	21.67052978829197
46-47	21.729865511470184	27.37110465995263	29.132064261672426	21.766965566904762
48-49	21.663952739042518	27.610097100781445	28.911860917791994	21.814089242384046
50-51	21.4648103573796	27.726077512870134	28.84868860946564	21.96042352028462
52-53	21.485861601223604	27.616496622444142	28.89753319234381	22.000108583988442
54-55	21.6998407072148	27.35759986531894	29.044160938434267	21.898398489031994
56-57	21.71269700683305	27.037092533260736	28.963524164196887	22.28668629570932
58-59	21.230005062025764	26.895960867504133	29.3383734131621	22.535660657308
60-61	21.62890361554784	27.50021075617259	29.311445876444107	21.559439751835463
62-63	20.908401995774252	27.931428583291083	29.34782524078527	21.812344180149392
64-65	20.6787496129174	28.259908113469688	28.951341369510253	22.110000904102662
66-67	20.755632268196123	28.921185652529957	28.407597448602978	21.915584630670946
68-69	20.976545612725843	28.838674922950453	28.25331646509286	21.931462999230845
70-71	21.02207123321009	28.03443807349521	28.28106325148136	22.662427441813342
72-73	21.3033253118483	27.23989376347423	28.42154490536647	23.035236019310997
74-75	21.4775700502332	27.327522580935774	28.57872298460323	22.616184384227793
76-77	21.799138961934965	27.265096741239248	27.72939060645546	23.20637369037032
78-79	22.083085536674744	27.271840979871598	27.19091012534808	23.45416335810558
80-81	21.883084455512073	27.829687630297816	27.315052579213884	22.972175334976225
82-83	21.912049708715266	27.499256939073852	27.344234792706608	23.24445855950427
84-85	21.939208128220546	27.985321083650334	27.330953374756824	22.744517413372296
86-87	22.616595809123893	27.851841001065637	27.39069873836044	22.140864451450028
88-89	22.264554259785065	28.09164171557135	27.735776572390193	21.9080274522534
90-91	22.228011238018514	27.546406543132342	27.546508005921368	22.67907421292778
92-93	22.216564324680515	27.022033071791938	27.83361727808249	22.92778532544505
94-95	21.34660733387941	27.539499066863566	28.61268655839504	22.50120704086199
96-97	21.491981363403063	27.38301667959347	28.87480340048923	22.25019855651424
98-99	22.64081386807834	29.276119482327683	29.186083384682377	18.8969832649116
100	29.11477977522831	40.41602242377279	30.469197800998906	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1373.0
1	1308.0
2	1591.5
3	2102.0
4	2624.5
5	3488.0
6	4341.0
7	5766.0
8	7127.0
9	7585.0
10	7553.0
11	7495.5
12	8323.5
13	10596.0
14	15239.0
15	20693.5
16	24509.0
17	26223.0
18	25934.0
19	24320.0
20	22205.0
21	20778.0
22	21232.0
23	24406.0
24	30696.5
25	39392.5
26	50668.5
27	64008.0
28	78167.5
29	93396.5
30	108645.5
31	123710.0
32	140586.0
33	158741.5
34	174621.5
35	190851.0
36	209619.5
37	224824.0
38	236834.5
39	245988.5
40	254574.0
41	262099.5
42	266652.5
43	272926.5
44	283218.5
45	296007.0
46	295533.0
47	288162.0
48	281776.5
49	271404.0
50	261449.5
51	248789.5
52	233431.0
53	215608.0
54	196010.0
55	177480.5
56	158234.5
57	139475.5
58	121669.5
59	102595.0
60	84410.5
61	67349.0
62	52422.5
63	40276.5
64	30238.5
65	22405.0
66	16455.5
67	12495.0
68	9543.0
69	7047.0
70	5193.0
71	3854.5
72	2768.5
73	1921.0
74	1308.0
75	889.5
76	585.5
77	366.5
78	239.5
79	155.5
80	102.0
81	71.5
82	55.0
83	37.0
84	22.0
85	15.0
86	9.0
87	7.0
88	6.5
89	5.5
90	5.5
91	4.5
92	2.5
93	2.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004017435040350898
3	0.003421097026548811
4	0.0
5	3.138621125274139E-5
6	1.7262416189007764E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.6480647246813702E-4
26-27	0.0019779660857307015
28-29	0.006957688615006901
30-31	0.0021625585001563727
32-33	0.004866284057383033
34-35	0.0026443276401117584
36-37	0.0010906059502186944
38-39	3.2029274757128014E-5
40-41	6.446271855077495E-5
42-43	0.002258204022722406
44-45	5.985698117301153E-4
46-47	4.2272002432214973E-4
48-49	0.0018177218667752273
50-51	4.990447944299158E-4
52-53	0.0
54-55	0.0043550413041285796
56-57	0.0465535941031208
58-59	0.06964257117006659
60-61	0.031346577005178865
62-63	0.013059417669322919
64-65	0.0013714219426812014
66-67	0.0
68-69	0.0019219555531240584
70-71	0.003775286953495526
72-73	0.012902782076412532
74-75	0.01552254828531908
76-77	0.02063539597058065
78-79	0.00593066147244303
80-81	0.0075716769443395495
82-83	0.032717913421130444
84-85	0.017916077876525292
86-87	0.012704377460729898
88-89	0.005824312082079294
90-91	3.043882744360624E-5
92-93	0.0
94-95	0.0
96-97	3.173518133641292E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	335.0
22-23	578.0
24-25	723.0
26-27	2298.0
28-29	5808.0
30-31	17823.0
32-33	22345.0
34-35	30773.0
36-37	46208.0
38-39	27588.0
40-41	49521.0
42-43	54511.0
44-45	64649.0
46-47	64970.0
48-49	59100.0
50-51	29794.0
52-53	4288.0
54-55	4258.0
56-57	3491.0
58-59	30522.0
60-61	57342.0
62-63	56546.0
64-65	61857.0
66-67	66515.0
68-69	67413.0
70-71	75925.0
72-73	57700.0
74-75	56634.0
76-77	59695.0
78-79	60925.0
80-81	60066.0
82-83	56406.0
84-85	55864.0
86-87	57412.0
88-89	59303.0
90-91	59933.0
92-93	59002.0
94-95	68059.0
96-97	217468.0
98-99	1504568.0
100-101	3033923.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.46838322694626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.72749831990409	81.14983473814344
2	6.111646241117439	10.813749236134065
3	1.3021349141768244	3.4559331180174624
4	0.4107235109285839	1.4534417986058723
5	0.1742123651125367	0.7706143139824291
6	0.09337047398824841	0.4956200924924386
7	0.052456308510167485	0.3248507362763894
8	0.03639426106490424	0.2575793148121182
9	0.02384659083970828	0.18987024033571484
>10	0.0660998000430667	0.8878984208213503
>50	8.711023923665573E-4	0.05719066033513184
>100	6.962614241226087E-4	0.11922742956212382
>500	4.9850497965168495E-5	0.024189900481332093
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5693105626370694E-5	0.0	0.0	0.0
3	0.0	1.5693105626370694E-5	0.0	0.0	0.0
4	0.0	4.707931687911208E-5	0.0	0.0	0.0
5	0.0	4.707931687911208E-5	0.0	1.5693105626370694E-5	0.0
6	0.0	4.707931687911208E-5	0.0	3.138621125274139E-5	0.0
7	0.0	4.707931687911208E-5	0.0	3.138621125274139E-5	0.0
8	0.0	6.277242250548278E-5	0.0	3.138621125274139E-5	0.0
9	0.0	7.846552813185348E-5	0.0	7.846552813185348E-5	0.0
10-11	0.0	7.846552813185348E-5	0.0	1.2554484501096555E-4	0.0
12-13	0.0	7.846552813185348E-5	0.0	1.6477760907689229E-4	0.0
14-15	0.0	1.2554484501096555E-4	0.0	1.7262416189007764E-4	0.0
16-17	0.0	1.4123795063733624E-4	0.0	2.1185692595600438E-4	0.0
18-19	0.0	1.80470714703263E-4	0.0	2.510896900219311E-4	0.0
20-21	0.0	2.1970347876918973E-4	0.0	2.5893624283511647E-4	0.0
22-23	0.0	2.667827956483018E-4	0.0	2.824759012746725E-4	0.0
24-25	0.0	2.746293484614872E-4	0.0	3.138621125274139E-4	0.0
26-27	0.0	2.824759012746725E-4	0.0	4.786397216043062E-4	0.0
28-29	0.0	2.824759012746725E-4	0.0	0.0011377501579118753	0.0
30-31	0.0	3.2955521815378457E-4	0.0	0.003232779759032363	0.0
32-33	0.0	3.2955521815378457E-4	0.0	0.007477764830965636	0.0
34-35	0.0	3.84481087846082E-4	0.0	0.013346986335228276	0.0
36-37	0.0	4.472535103515648E-4	0.0	0.027062760652676262	0.0
38-39	0.0	4.551000631647501E-4	0.0	0.23699728116945024	0.0
40-41	0.0	4.7079316879112084E-4	0.0	0.4545978837847063	0.0
42-43	0.0	4.7079316879112084E-4	0.0	0.4832221084472064	0.0
44-45	7.846552813185347E-6	5.257190384834183E-4	0.0	0.5222979414568695	0.0
46-47	1.5693105626370694E-5	5.806449081757157E-4	0.0	0.5647477921762021	0.0
48-49	1.5693105626370694E-5	5.88491460988901E-4	0.0	0.6062482100051394	0.0
50-51	1.5693105626370694E-5	6.591104363075691E-4	0.0	0.6477800140453296	0.0
52-53	1.5693105626370694E-5	6.591104363075691E-4	0.0	1.242509484520713	0.0
54-55	1.5693105626370694E-5	6.669569891207545E-4	0.0	2.04771488765698	0.0
56-57	1.5693105626370694E-5	7.061897531866813E-4	0.0	2.9281138064020027	0.0
58-59	1.5693105626370694E-5	7.061897531866813E-4	0.0	3.8207062682187147	0.0
60-61	1.5693105626370694E-5	7.140363059998666E-4	0.0	4.0546747800022755	0.0
62-63	1.5693105626370694E-5	7.21882858813052E-4	0.0	4.063211829463022	0.0
64-65	1.5693105626370694E-5	8.160414925712761E-4	0.0	4.070940683984009	0.0
66-67	1.5693105626370694E-5	8.160414925712761E-4	0.0	4.078371369498095	0.0
68-69	1.5693105626370694E-5	8.160414925712761E-4	0.0	4.085621584297479	0.0
70-71	1.5693105626370694E-5	8.238880453844614E-4	0.0	4.091741895491763	0.0
72-73	1.5693105626370694E-5	8.317345981976468E-4	0.0	4.09666168410563	0.0
74-75	1.5693105626370694E-5	8.395811510108321E-4	0.0	4.100333870822201	0.0
76-77	1.5693105626370694E-5	8.631208094503882E-4	0.0	4.103715735084684	0.0
78-79	1.5693105626370694E-5	8.866604678899443E-4	0.0	4.106140319903958	0.0
80-81	1.5693105626370694E-5	9.02353573516315E-4	0.0	4.107058366583101	0.0
82-83	1.5693105626370694E-5	9.102001263295003E-4	0.0	4.107615471832837	0.0
84-85	1.5693105626370694E-5	9.102001263295003E-4	0.0	4.108039185684749	0.0
86-87	1.5693105626370694E-5	9.102001263295003E-4	0.0	4.108196116741013	0.0
88	1.5693105626370694E-5	9.729725488349831E-4	0.0	4.108203963293826	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8845	0.0	41.600613	1
ATCTCGT	11145	0.0	38.47783	94
GTATCAA	14960	0.0	33.553043	1
TATCTCG	13110	0.0	32.91457	94
TCAACGC	18215	0.0	28.30202	4
ATCAACG	18215	0.0	28.279554	3
CAACGCA	18450	0.0	27.826262	5
AACGCAG	19010	0.0	27.051302	6
TATCAAC	19480	0.0	26.553764	2
ACGCAGA	21685	0.0	23.71433	7
CGCAGAG	22055	0.0	23.335777	8
GCAGAGT	25470	0.0	20.307133	9
CTATCTC	15380	0.0	19.852816	92-93
GTACATG	17630	0.0	19.616417	1
GAGTACT	15355	0.0	19.473747	12-13
TACATGG	18030	0.0	19.393562	2
ACATGGG	17590	0.0	19.22467	3
GCCGTCT	8375	0.0	18.983328	90-91
AGAGTAC	22595	0.0	18.900154	10-11
CCGTCTT	8455	0.0	18.634308	90-91
>>END_MODULE