FastQCFastQC Report
Fri 27 May 2016
SRR2139611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6874386
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100200.046.5510371
GTATCAA189100.038.9859121
CTTATAC86750.033.6603281
TCAACGC224400.032.3582844
ATCAACG227500.031.8623223
CAACGCA227800.031.8566935
TATCAAC233050.031.1966272
AACGCAG235500.030.9049726
TATACAC106750.028.982153
ACGCAGA266950.027.2480897
CGCAGAG274350.026.4821918
TTATACA116500.025.7932872
GCAGAGT309850.023.516579
ACATCTC130850.022.6379388
ACACATC131850.022.2731236
TACACAT141550.021.916235
GTGGTAT53450.021.4395391
TGGTATC52100.020.6101972
GAGTACT187350.020.49981912-13
CACATCT144750.020.2588427
Produced by FastQC (version 0.11.5)