##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139611_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6874386
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.13872278920619	32.0	25.0	33.0	18.0	33.0
2	31.02583736205677	33.0	31.0	33.0	27.0	33.0
3	30.45691862516885	31.0	29.0	33.0	27.0	33.0
4	31.70002848254375	33.0	31.0	33.0	29.0	33.0
5	32.04348213789566	33.0	32.0	33.0	31.0	33.0
6	35.0631471668888	37.0	34.0	38.0	29.0	38.0
7	36.47256758639972	38.0	37.0	38.0	34.0	38.0
8	36.03979220835141	38.0	36.0	38.0	33.0	38.0
9	36.6727706299879	38.0	37.0	38.0	34.0	38.0
10-11	36.98385804928615	38.0	38.0	38.0	35.0	38.0
12-13	37.131325474013245	38.0	38.0	38.0	36.0	38.0
14-15	37.16490832781284	38.0	38.0	38.0	36.0	38.0
16-17	37.23691766799246	38.0	38.0	38.0	36.0	38.0
18-19	37.30126879695146	38.0	38.0	38.0	36.5	38.0
20-21	37.348386143154045	38.0	38.0	38.0	37.0	38.0
22-23	37.376727762773626	38.0	38.0	38.0	37.0	38.0
24-25	37.40154972742233	38.0	38.0	38.0	37.0	38.0
26-27	37.380436050249244	38.0	38.0	38.0	37.0	38.0
28-29	37.3631753339725	38.0	38.0	38.0	37.0	38.0
30-31	37.367365611221004	38.0	38.0	38.0	37.0	38.0
32-33	37.350351743049	38.0	38.0	38.0	37.0	38.0
34-35	37.32624371172119	38.0	38.0	38.0	37.0	38.0
36-37	37.305244730060366	38.0	38.0	38.0	37.0	38.0
38-39	37.275225584403216	38.0	38.0	38.0	37.0	38.0
40-41	37.29773113055117	38.0	38.0	38.0	37.0	38.0
42-43	37.26368252242537	38.0	38.0	38.0	37.0	38.0
44-45	37.22318515100703	38.0	38.0	38.0	37.0	38.0
46-47	37.192860195529235	38.0	38.0	38.0	37.0	38.0
48-49	37.141163129111945	38.0	38.0	38.0	36.5	38.0
50-51	37.10114585933977	38.0	38.0	38.0	36.0	38.0
52-53	37.08451638789813	38.0	38.0	38.0	36.0	38.0
54-55	37.05437002330886	38.0	38.0	38.0	36.0	38.0
56-57	36.99749637863283	38.0	38.0	38.0	36.0	38.0
58-59	36.96735066508678	38.0	38.0	38.0	36.0	38.0
60-61	36.9841358696863	38.0	38.0	38.0	36.0	38.0
62-63	36.989852068933246	38.0	38.0	38.0	36.0	38.0
64-65	36.99399893312871	38.0	38.0	38.0	36.0	38.0
66-67	36.98809121833703	38.0	38.0	38.0	36.0	38.0
68-69	36.97196817410142	38.0	38.0	38.0	36.0	38.0
70-71	36.96459571090858	38.0	38.0	38.0	36.0	38.0
72-73	36.956024759181574	38.0	38.0	38.0	36.0	38.0
74-75	36.93205391319176	38.0	38.0	38.0	36.0	38.0
76-77	36.90889243355485	38.0	38.0	38.0	35.0	38.0
78-79	36.90964507091422	38.0	38.0	38.0	35.0	38.0
80-81	36.90794320850085	38.0	38.0	38.0	35.0	38.0
82-83	36.85431414366951	38.0	38.0	38.0	35.0	38.0
84-85	36.832397497982136	38.0	38.0	38.0	35.0	38.0
86-87	36.830963919394016	38.0	38.0	38.0	35.0	38.0
88-89	36.81607341626909	38.0	38.0	38.0	35.0	38.0
90-91	36.786598908094255	38.0	38.0	38.0	35.0	38.0
92-93	36.773168792470074	38.0	38.0	38.0	35.0	38.0
94-95	36.748747873058875	38.0	38.0	38.0	35.0	38.0
96-97	36.71267131259697	38.0	38.0	38.0	34.0	38.0
98-99	36.68733783322	38.0	38.0	38.0	34.0	38.0
100	35.234195444455686	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	9.0
19	11.0
20	31.0
21	101.0
22	384.0
23	1262.0
24	3093.0
25	6426.0
26	11551.0
27	19606.0
28	30570.0
29	44873.0
30	60387.0
31	78979.0
32	104683.0
33	143884.0
34	220816.0
35	415622.0
36	1240340.0
37	4491755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.124923738643716	18.933138174085656	14.18325942127777	25.75867866599286
2	14.302882552140842	20.27355921127475	42.83464392815921	22.588914308425196
3	18.112322681932497	25.42600560248865	30.600161068639213	25.86151064693964
4	12.373599620387916	17.802564476303775	38.930938704925794	30.892897198382517
5	13.4289453219213	36.82699041164719	35.77640870117488	13.967655565256628
6	29.86726622776048	37.70859026626761	18.577956761660207	13.846186744311689
7	26.44007770293958	32.58139708768172	22.734277650396706	18.244247558981993
8	24.08031786402451	36.66398715463461	21.19163806047551	18.06405692086537
9	25.312500636420477	16.906251700151838	21.30689199006282	36.47435567336487
10-11	23.999459733567477	26.595160644165166	28.951582003105443	20.453797619161914
12-13	25.013019344564007	24.14750931937776	28.993978516772263	21.84549281928597
14-15	22.198331603724316	25.501157485192135	26.574329692862754	25.726181218220802
16-17	21.722798225179673	28.850002021998765	27.233035794033096	22.194163958788465
18-19	21.78673848108035	27.65188774677477	29.172118062616793	21.389255709528094
20-21	22.445118086932272	26.742526242732556	29.148095210282627	21.664260460052546
22-23	22.136575212517002	26.93648687486875	29.0557102571692	21.871227655445054
24-25	22.141255413715104	27.0156451464936	29.20344408712928	21.63965535266201
26-27	22.111585528240187	27.11395761388558	29.206013455401152	21.56844340247308
28-29	22.034819138099333	26.926357032989362	29.230268156531135	21.80855567238017
30-31	22.021060235105775	27.086303588850857	29.361848466466366	21.530787709577005
32-33	22.00941645087361	27.255097704698102	29.119821423119607	21.615664421308683
34-35	22.30084718789161	26.928496861402945	29.246936689569964	21.52371926113548
36-37	21.835694455274528	27.163512537314748	29.154505503236027	21.84628750417469
38-39	21.70369579892803	26.900779370864164	29.44076880598347	21.954756024224334
40-41	21.970439526041964	26.78268004018432	29.368349947430534	21.878530486343188
42-43	22.067437091180032	26.796423216159898	29.156236980076688	21.979902712583385
44-45	21.862689433448644	27.12593564084936	29.040889145266117	21.97048578043588
46-47	22.232763914156152	26.861031972143085	28.581166069787223	22.325038043913544
48-49	22.1169959273175	26.974064869039676	28.525873052783073	22.383066150859747
50-51	21.900020452778726	27.119841252284445	28.666684519186997	22.313453775749835
52-53	22.05458009098324	27.11117230978089	28.805033157924083	22.029214441311794
54-55	22.06820825047842	27.115396657919394	28.474788279362933	22.341606812239252
56-57	22.238791273627044	27.370788265628214	28.1539479190572	22.236472541687544
58-59	21.860800317099976	27.584094553447564	28.59930294058179	21.95580218887067
60-61	22.146492841232458	27.919762518832226	28.073560523090812	21.860184116844504
62-63	21.812262721312557	28.200180481389538	28.090825203733534	21.89673159356438
64-65	21.788442981719488	28.07450183984514	28.13949166018726	21.99756351824811
66-67	21.86555721717049	28.0765305745676	27.92274522204575	22.135166986216156
68-69	21.889612821239734	27.857824923179752	28.158433929381598	22.094128326198913
70-71	21.969592962047443	27.43415512557626	28.344364683277863	22.251887229098433
72-73	22.161065542629785	27.299904629311953	28.244102837878177	22.29492699018008
74-75	22.01240683277568	27.42036707586662	28.20257474010825	22.36465135124945
76-77	22.217719443993435	27.341334302424773	28.0933275054518	22.347618748129992
78-79	22.16384962430527	27.36551429165484	28.047431874905264	22.423204209134624
80-81	22.16239177365649	27.459848323098747	27.964017580304475	22.41374232294029
82-83	22.314360261724868	27.40668784285434	28.020449867777753	22.25850202764304
84-85	22.357104911312796	27.41971422581012	27.93379898054596	22.28938188233112
86-87	22.214895184604437	27.44366855360955	28.0847289771234	22.256707284662607
88-89	22.31523297571252	27.44919718080813	28.000463996968357	22.235105846510997
90-91	22.355837424330574	27.51642880571164	27.937054125611738	22.19067964434604
92-93	22.433777596759676	27.48158944827169	27.92879886797583	22.1558340869928
94-95	22.240468065675394	27.51565772070648	27.990203949376465	22.253670264241666
96-97	22.405810624374016	27.592391218604646	28.027212044261514	21.97458611275983
98-99	23.422477820895573	29.531628004753696	28.201634040114808	18.844260134235924
100	29.856755262995588	40.47044057846395	29.672804158540462	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1814.0
1	1624.0
2	1890.5
3	2530.5
4	3216.5
5	4493.5
6	5661.0
7	7320.5
8	9487.0
9	10552.0
10	10041.5
11	9588.0
12	10616.0
13	13375.5
14	18882.5
15	25014.0
16	29495.0
17	31724.5
18	30956.0
19	28120.0
20	24801.0
21	22259.0
22	21903.0
23	24432.0
24	29645.0
25	38061.5
26	49909.0
27	62883.0
28	75599.5
29	90094.0
30	105167.5
31	119389.0
32	137504.0
33	158659.5
34	176985.0
35	194863.5
36	214917.0
37	230640.0
38	242839.5
39	253054.0
40	262906.0
41	271099.5
42	276678.5
43	291558.0
44	308646.5
45	312730.0
46	305499.0
47	296931.5
48	290806.5
49	285738.0
50	279594.5
51	270779.5
52	259225.0
53	244256.5
54	227125.5
55	209886.5
56	191504.0
57	172475.0
58	153493.0
59	132191.5
60	111090.0
61	90558.5
62	71144.0
63	55097.0
64	41639.0
65	30847.5
66	22562.0
67	17053.5
68	12986.5
69	9664.5
70	7183.5
71	5025.5
72	3414.0
73	2280.0
74	1559.5
75	1054.0
76	683.0
77	416.5
78	260.5
79	170.0
80	113.5
81	80.0
82	53.5
83	35.0
84	27.0
85	22.0
86	16.5
87	11.0
88	8.5
89	8.5
90	8.5
91	6.0
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005935075510743796
3	0.004334932603435419
4	0.0
5	5.818701481121368E-5
6	3.491220888672821E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.4193108124427993E-4
26-27	0.0017608423869979396
28-29	0.006849822625262582
30-31	0.002608849889255051
32-33	0.005682093721534756
34-35	0.0028275179615148433
36-37	0.0011843476903491332
38-39	2.220134144945306E-5
40-41	8.194185510251559E-5
42-43	0.0023245974959435773
44-45	5.593104095678965E-4
46-47	4.347856929877535E-4
48-49	0.0021705723499101173
50-51	5.278108586683193E-4
52-53	0.0
54-55	0.004662894395602638
56-57	0.0467418828144725
58-59	0.07144855320080556
60-61	0.033167129163856675
62-63	0.014415266801274274
64-65	0.001782198872040585
66-67	0.0
68-69	0.00227430101120794
70-71	0.003958827514225018
72-73	0.013620070080982202
74-75	0.01619961539594075
76-77	0.021705111461666933
78-79	0.006599481289628999
80-81	0.008468051589948298
82-83	0.034322844199792056
84-85	0.01943701525632153
86-87	0.014033829001530437
88-89	0.0065175390332056025
90-91	6.599518763091796E-5
92-93	0.0
94-95	0.0
96-97	9.799219864508146E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	74.0
20-21	446.0
22-23	1005.0
24-25	802.0
26-27	2212.0
28-29	5229.0
30-31	14318.0
32-33	18764.0
34-35	25566.0
36-37	38690.0
38-39	44499.0
40-41	43355.0
42-43	49716.0
44-45	59241.0
46-47	60271.0
48-49	55726.0
50-51	48827.0
52-53	45546.0
54-55	48796.0
56-57	49640.0
58-59	52366.0
60-61	53209.0
62-63	54000.0
64-65	60264.0
66-67	66768.0
68-69	67796.0
70-71	81309.0
72-73	56365.0
74-75	54241.0
76-77	56424.0
78-79	58484.0
80-81	58293.0
82-83	54482.0
84-85	54767.0
86-87	56191.0
88-89	58189.0
90-91	59615.0
92-93	58503.0
94-95	67812.0
96-97	206803.0
98-99	1585764.0
100-101	3340018.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.54678834793827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.68283993598055	78.48308554729273
2	6.724341451537195	11.639403127709153
3	1.4559140382118327	3.780140523537348
4	0.5312961244046821	1.8392789291572766
5	0.23518793461373722	1.0177380199501929
6	0.11617515240098383	0.6032751795682453
7	0.07215349880699917	0.4371257512868802
8	0.04710687846790449	0.32615592323950243
9	0.033426655736061524	0.2603672729251482
>10	0.0988306247639644	1.3353233572624201
>50	0.002003178021192745	0.11257202406612846
>100	6.205240437040769E-4	0.10014117733657232
>500	7.800228888359542E-5	0.0417202314875435
>1k	2.6000722297733345E-5	0.023672935180944103
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4546753702803422E-5	0.0	1.4546753702803422E-5	0.0
3	0.0	1.4546753702803422E-5	0.0	1.4546753702803422E-5	0.0
4	0.0	1.4546753702803422E-4	0.0	1.4546753702803422E-5	0.0
5	0.0	1.4546753702803422E-4	0.0	1.4546753702803422E-5	0.0
6	5.818701481121369E-5	1.4546753702803422E-4	0.0	1.4546753702803422E-5	0.0
7	5.818701481121369E-5	1.4546753702803422E-4	0.0	2.9093507405606844E-5	0.0
8	5.818701481121369E-5	1.7456104443364106E-4	0.0	2.9093507405606844E-5	0.0
9	5.818701481121369E-5	2.4729481294765815E-4	0.0	2.9093507405606844E-5	0.0
10-11	5.818701481121369E-5	2.4729481294765815E-4	0.0	3.6366884257008555E-5	0.0
12-13	5.818701481121369E-5	2.4729481294765815E-4	0.0	4.3640261108410266E-5	0.0
14-15	5.818701481121369E-5	3.782155962728889E-4	0.0	5.091363795981198E-5	0.0
16-17	1.1637402962242738E-4	4.073091036784958E-4	0.0	5.818701481121369E-5	0.0
18-19	1.1637402962242738E-4	4.2912923423270093E-4	0.0	7.273376851401711E-5	0.0
20-21	1.1637402962242738E-4	4.5094936478690605E-4	0.0	7.273376851401711E-5	1.4546753702803422E-5
22-23	1.1637402962242738E-4	4.873162490439146E-4	0.0	1.0182727591962395E-4	1.4546753702803422E-5
24-25	1.1637402962242738E-4	5.091363795981197E-4	0.0	1.7456104443364106E-4	1.4546753702803422E-5
26-27	1.1637402962242738E-4	5.091363795981197E-4	0.0	2.4002143609625648E-4	1.4546753702803422E-5
28-29	1.1637402962242738E-4	5.091363795981197E-4	0.0	6.400571629233506E-4	2.1820130554205133E-5
30-31	1.1637402962242738E-4	5.382298870037266E-4	0.0	0.002291113708191539	2.9093507405606844E-5
32-33	1.1637402962242738E-4	5.5277664070653E-4	0.0	0.005687780697796138	2.9093507405606844E-5
34-35	1.1637402962242738E-4	7.127909314373676E-4	0.0	0.010102720446596975	2.9093507405606844E-5
36-37	1.1637402962242738E-4	8.728052221682053E-4	0.0	0.01755793171928373	2.9093507405606844E-5
38-39	1.1637402962242738E-4	9.018987295738121E-4	0.0	0.03404667704141141	2.9093507405606844E-5
40-41	1.1637402962242738E-4	9.746324980878292E-4	0.0	0.05201191786437363	2.9093507405606844E-5
42-43	1.1637402962242738E-4	9.746324980878292E-4	0.0	0.06344566627477713	2.9093507405606844E-5
44-45	1.1637402962242738E-4	0.001032819512899043	0.0	0.07682140630450487	2.9093507405606844E-5
46-47	1.1637402962242738E-4	0.0010910065277102566	0.0	0.09111359181750923	2.9093507405606844E-5
48-49	1.236474064738291E-4	0.0010982799045616584	0.0	0.10509302212590332	2.9093507405606844E-5
50-51	1.4546753702803422E-4	0.001207380557332684	0.0	0.11899971866578338	2.9093507405606844E-5
52-53	1.4546753702803422E-4	0.001207380557332684	0.0	0.1534755249414275	2.9093507405606844E-5
54-55	1.4546753702803422E-4	0.001207380557332684	0.0	0.19775584321276113	2.9093507405606844E-5
56-57	1.4546753702803422E-4	0.001207380557332684	0.0	0.2571866054655645	2.9093507405606844E-5
58-59	1.4546753702803422E-4	0.0012219273110354876	0.0	0.29649920734739077	2.9093507405606844E-5
60-61	1.4546753702803422E-4	0.0012582941952924958	0.0	0.31265337733435394	2.9093507405606844E-5
62-63	1.7456104443364106E-4	0.0012801143258467011	0.0	0.3258763764502023	2.9093507405606844E-5
64-65	1.7456104443364106E-4	0.001432855239726137	0.0	0.33170235130817505	2.9093507405606844E-5
66-67	1.818344212850428E-4	0.0014401286165775387	0.0	0.337586513180959	3.6366884257008555E-5
68-69	1.8910779813644449E-4	0.001483768877685949	0.0	0.3432888406324579	4.3640261108410266E-5
70-71	1.8910779813644449E-4	0.0014983156313887523	0.0	0.3480092622090176	4.3640261108410266E-5
72-73	2.1820130554205133E-4	0.0014983156313887523	0.0	0.35201689285413995	4.3640261108410266E-5
74-75	2.1820130554205133E-4	0.0015201357619429574	0.0	0.35487532995674087	5.091363795981198E-5
76-77	2.1820130554205133E-4	0.0015710493999027695	0.0	0.35812652940931744	5.818701481121369E-5
78-79	2.1820130554205133E-4	0.0016001429073083764	0.0	0.3601485281740071	5.818701481121369E-5
80-81	2.1820130554205133E-4	0.0016001429073083764	0.0	0.36077403858322765	5.818701481121369E-5
82-83	2.1820130554205133E-4	0.0016219630378625815	0.0	0.36126135483227156	5.818701481121369E-5
84-85	2.1820130554205133E-4	0.0016510565452681884	0.0	0.36148682951466504	6.54603916626154E-5
86-87	2.1820130554205133E-4	0.0016874234295251968	0.0	0.36160320354428743	7.273376851401711E-5
88	2.1820130554205133E-4	0.0017456104443364106	0.0	0.36160320354428743	7.273376851401711E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10020	0.0	46.551037	1
GTATCAA	18910	0.0	38.985912	1
CTTATAC	8675	0.0	33.660328	1
TCAACGC	22440	0.0	32.358284	4
ATCAACG	22750	0.0	31.862322	3
CAACGCA	22780	0.0	31.856693	5
TATCAAC	23305	0.0	31.196627	2
AACGCAG	23550	0.0	30.904972	6
TATACAC	10675	0.0	28.98215	3
ACGCAGA	26695	0.0	27.248089	7
CGCAGAG	27435	0.0	26.482191	8
TTATACA	11650	0.0	25.793287	2
GCAGAGT	30985	0.0	23.51657	9
ACATCTC	13085	0.0	22.637938	8
ACACATC	13185	0.0	22.273123	6
TACACAT	14155	0.0	21.91623	5
GTGGTAT	5345	0.0	21.439539	1
TGGTATC	5210	0.0	20.610197	2
GAGTACT	18735	0.0	20.499819	12-13
CACATCT	14475	0.0	20.258842	7
>>END_MODULE