FastQCFastQC Report
Fri 27 May 2016
SRR2139610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7121370
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124800.051.5675351
GTATCAA230450.037.289471
TGGTATC52350.030.6937962
TCAACGC278500.030.3136064
ATCAACG280700.030.0033743
TATCAAC287550.029.521892
CAACGCA286100.029.5083525
GTGGTAT57700.028.932821
AACGCAG294050.028.7389456
ACGCAGA333400.025.3593277
CGCAGAG339150.024.9416878
GCAGAGT384850.022.0883589
AAAGGCG26100.021.13418294
GAGTACT211300.020.42137312-13
TTAGCCG6755.456968E-1219.77068794
AGGACCG15950.019.6230955
GGACCGA13450.019.547236
CAGAGTA379550.019.48862310-11
GTACATG300750.019.4696711
TACATGG301550.018.9336072
Produced by FastQC (version 0.11.5)