##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139610_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7121370
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.377428640837365	32.0	25.0	33.0	18.0	33.0
2	31.165662927217657	33.0	31.0	33.0	27.0	33.0
3	30.560988967010562	31.0	29.0	33.0	27.0	33.0
4	31.768920446487122	33.0	31.0	33.0	29.0	33.0
5	32.108125121991975	33.0	32.0	33.0	31.0	33.0
6	35.18646889573214	37.0	35.0	38.0	29.0	38.0
7	36.58264505284798	38.0	37.0	38.0	34.0	38.0
8	36.1738838172992	38.0	36.0	38.0	33.0	38.0
9	36.76283425801496	38.0	38.0	38.0	34.0	38.0
10-11	37.05885728448318	38.0	38.0	38.0	35.5	38.0
12-13	37.19480415987373	38.0	38.0	38.0	36.0	38.0
14-15	37.22599654280005	38.0	38.0	38.0	36.0	38.0
16-17	37.297799650909866	38.0	38.0	38.0	36.5	38.0
18-19	37.349582804993986	38.0	38.0	38.0	37.0	38.0
20-21	37.38768039773473	38.0	38.0	38.0	37.0	38.0
22-23	37.410650036211024	38.0	38.0	38.0	37.0	38.0
24-25	37.433192116128005	38.0	38.0	38.0	37.0	38.0
26-27	37.411551713403554	38.0	38.0	38.0	37.0	38.0
28-29	37.39671500018427	38.0	38.0	38.0	37.0	38.0
30-31	37.40018004458941	38.0	38.0	38.0	37.0	38.0
32-33	37.389631851411465	38.0	38.0	38.0	37.0	38.0
34-35	37.36818948284802	38.0	38.0	38.0	37.0	38.0
36-37	37.348856140741916	38.0	38.0	38.0	37.0	38.0
38-39	37.33156508312729	38.0	38.0	38.0	37.0	38.0
40-41	37.333457691851876	38.0	38.0	38.0	37.0	38.0
42-43	37.29403884047802	38.0	38.0	38.0	37.0	38.0
44-45	37.25193550509749	38.0	38.0	38.0	37.0	38.0
46-47	37.22082771818633	38.0	38.0	38.0	37.0	38.0
48-49	37.1692826258832	38.0	38.0	38.0	37.0	38.0
50-51	37.13887995881703	38.0	38.0	38.0	37.0	38.0
52-53	37.12885622936256	38.0	38.0	38.0	36.0	38.0
54-55	37.105793503438974	38.0	38.0	38.0	36.0	38.0
56-57	37.053434431646735	38.0	38.0	38.0	36.0	38.0
58-59	37.03029281734398	38.0	38.0	38.0	36.0	38.0
60-61	37.04892926046965	38.0	38.0	38.0	36.0	38.0
62-63	37.05882395142382	38.0	38.0	38.0	36.0	38.0
64-65	37.06846033507464	38.0	38.0	38.0	36.0	38.0
66-67	37.06805075383683	38.0	38.0	38.0	36.0	38.0
68-69	37.0581726191279	38.0	38.0	38.0	36.0	38.0
70-71	37.05273741769189	38.0	38.0	38.0	36.0	38.0
72-73	37.046266218022865	38.0	38.0	38.0	36.0	38.0
74-75	37.02775516152838	38.0	38.0	38.0	36.0	38.0
76-77	37.00344576111822	38.0	38.0	38.0	36.0	38.0
78-79	37.008270250608945	38.0	38.0	38.0	36.0	38.0
80-81	37.00473942519294	38.0	38.0	38.0	36.0	38.0
82-83	36.95194937535315	38.0	38.0	38.0	36.0	38.0
84-85	36.93190426607737	38.0	38.0	38.0	35.0	38.0
86-87	36.9337912415928	38.0	38.0	38.0	35.0	38.0
88-89	36.91629746236471	38.0	38.0	38.0	35.0	38.0
90-91	36.88831051471517	38.0	38.0	38.0	35.0	38.0
92-93	36.872429283067866	38.0	38.0	38.0	35.0	38.0
94-95	36.84667841263243	38.0	38.0	38.0	35.0	38.0
96-97	36.807717230417396	38.0	38.0	38.0	35.0	38.0
98-99	36.77790428908514	38.0	38.0	38.0	35.0	38.0
100	35.352149027435374	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	9.0
20	26.0
21	100.0
22	375.0
23	1284.0
24	3006.0
25	6141.0
26	11423.0
27	18936.0
28	29458.0
29	43203.0
30	58351.0
31	76941.0
32	101133.0
33	139623.0
34	213193.0
35	403009.0
36	1219443.0
37	4795713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50006248797633	19.20466146261183	14.413237902257572	24.88203814715427
2	14.346941925162692	20.466560229811485	43.123412873560575	22.06308497146525
3	18.10884531808246	25.967023532111288	30.697515179583768	25.22661597022249
4	12.284139147383158	17.791871507869974	39.664025320970545	30.259964023776327
5	13.082511463397031	37.296798000157835	35.827486371286845	13.793204165158294
6	29.28794640788413	38.07624289003792	18.90833558712075	13.7274751149572
7	26.26483387325753	33.015192301481314	23.138469142875596	17.58150468238555
8	24.104491130217923	36.65355121275822	21.2909032952929	17.951054361730957
9	25.653659338020635	16.997866983459645	21.697299817310434	35.65117386120929
10-11	23.876248530830445	26.901509119733984	29.042410378901813	20.17983197053376
12-13	24.90895712482289	24.73671498602095	29.168460563065814	21.185867326090346
14-15	22.24199837952529	25.959920633248938	27.094161657096876	24.703919330128894
16-17	21.259827814030167	29.187017385699658	27.859084698590298	21.694070101679873
18-19	21.233786476478542	27.98455212971661	30.020108490360702	20.76155290344414
20-21	22.206579990631518	27.03238166670529	29.96041920806111	20.800619134602083
22-23	21.94447812520241	26.966148004849465	29.8233378288364	21.266036041111725
24-25	21.587199087094568	27.266451814410093	29.94584191319458	21.20050718530076
26-27	21.45657112709646	27.38386079503801	29.916580880249473	21.24298719761606
28-29	21.300206610073545	27.250059095249448	30.10768100394899	21.342053290728018
30-31	21.71458100761666	27.157708731398845	29.951526501127752	21.17618375985674
32-33	21.38673198937122	27.388156616324576	29.863834302667136	21.361277091637067
34-35	21.575079783689286	27.293040164043443	29.916784331325918	21.215095720941353
36-37	21.534565485611733	27.239856642566046	29.791433805790913	21.4341440660313
38-39	21.29942926143559	27.365883753881253	29.926571575373146	21.40811540931001
40-41	21.552185041688475	27.18831862381585	29.94452309330261	21.31497324119307
42-43	21.597700032943905	27.200102424142486	29.629046950495315	21.573150592418298
44-45	21.49448558864305	27.470465262688016	29.481256474528482	21.55379267414045
46-47	21.72805949709689	27.404689829800542	29.078134943349294	21.789115729753274
48-49	21.616641622119563	27.552218087530143	28.993749178854465	21.837391111495826
50-51	21.586505229143818	27.7157138330075	28.77588465183234	21.92189628601634
52-53	21.79884360317694	27.738092519588324	28.64711290227216	21.815950974962572
54-55	21.871480412312923	27.736489727816355	28.546082637097598	21.845947222773123
56-57	21.840469620019917	27.844396276387677	28.35374489325974	21.961389210332662
58-59	21.63755940273418	27.78300756451579	28.702921733602178	21.876511299147854
60-61	21.817897658221202	28.04276953164264	28.43404879029429	21.705284019841862
62-63	21.61161056643333	28.095894991517827	28.548955156823304	21.743539285225534
64-65	21.588479559039662	27.947920231440282	28.5744429050916	21.889157304428455
66-67	21.697446614219505	28.036484544160324	28.298198490759013	21.96787035086116
68-69	21.703865979918664	27.945771643088364	28.5054976664881	21.84486471050487
70-71	21.75247132493171	27.665635985911695	28.596794294980683	21.985098394175914
72-73	21.85246180347712	27.75476236897892	28.43807597583657	21.95469985170739
74-75	21.720894247447262	27.860822644471366	28.368000309679324	22.05028279840204
76-77	21.859653862796403	27.82191542860658	28.273513471571043	22.04491723702597
78-79	21.875502763497575	27.781689879386263	28.21214383128857	22.13066352582759
80-81	21.834882680562362	27.96734992224702	28.090102716982813	22.107664680207805
82-83	22.018399488049536	27.88811918357526	28.14337874260502	21.950102585770185
84-85	21.985062041490462	27.919481408163893	28.044814791574396	22.050641758771253
86-87	21.85394072360818	28.059029243202765	28.19101475814451	21.896015275044547
88-89	21.975352105085218	27.9830081874397	28.110837637196628	21.930802070278453
90-91	22.01877633435716	28.05712547127137	28.074754094003186	21.849344100368285
92-93	22.104062618853416	28.046021965361046	28.00966171313511	21.840253702650433
94-95	21.906845109981827	28.032656606992944	28.079471682089896	21.981026600935326
96-97	22.044467998892518	28.129249356444685	28.137478229629725	21.688804415033072
98-99	22.98490060521921	30.033838729556507	28.401109060268208	18.58015160495608
100	29.24564579576774	40.97016723436906	29.784186969863203	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2633.0
1	2197.5
2	1867.0
3	2313.0
4	3383.5
5	4933.0
6	5970.5
7	6300.5
8	6194.0
9	6044.0
10	6207.5
11	7273.0
12	9500.0
13	14619.5
14	23352.5
15	33052.0
16	40659.5
17	43946.0
18	43849.5
19	39990.0
20	34813.5
21	30887.5
22	28960.5
23	30795.0
24	36496.5
25	46078.0
26	59610.0
27	74884.5
28	89189.0
29	105785.5
30	123784.5
31	140770.0
32	163224.5
33	189281.0
34	210576.5
35	231869.5
36	256689.5
37	272756.0
38	283825.0
39	291758.5
40	297476.0
41	299756.5
42	298479.5
43	306813.0
44	313988.0
45	311785.5
46	304185.5
47	297231.5
48	292965.5
49	286768.0
50	278053.0
51	267407.0
52	254033.0
53	237925.5
54	220380.0
55	202888.5
56	184476.0
57	165717.5
58	147705.5
59	128216.5
60	107686.0
61	87303.0
62	68142.0
63	52415.5
64	40317.5
65	30446.0
66	22052.5
67	16292.5
68	12208.5
69	8882.0
70	6428.5
71	4498.5
72	3067.0
73	2079.0
74	1385.5
75	910.5
76	601.0
77	390.0
78	264.0
79	176.5
80	128.0
81	98.0
82	74.5
83	52.5
84	32.5
85	20.5
86	15.5
87	11.5
88	7.5
89	5.0
90	3.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005659023474415738
3	0.00410033462662381
4	0.0
5	1.1233793497599478E-4
6	3.3701380492798434E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.6863988210947975E-4
26-27	0.0016095676637996915
28-29	0.00628715637332347
30-31	0.0021408106968423325
32-33	0.005189564188872176
34-35	0.0025082899336585657
36-37	9.471613574118367E-4
38-39	3.586526824638349E-5
40-41	3.6149301241236866E-5
42-43	0.0021932975740817226
44-45	5.811501520590407E-4
46-47	3.272984215512919E-4
48-49	0.001782328774231587
50-51	3.499905768841419E-4
52-53	0.0
54-55	0.004661218599208415
56-57	0.04659665192919375
58-59	0.07093743828648416
60-61	0.033204161662812016
62-63	0.014062349861421715
64-65	0.0015321754813618596
66-67	0.0
68-69	0.0024011979977010198
70-71	0.004009777441312898
72-73	0.013717043627548678
74-75	0.01632807932025428
76-77	0.02159570601884208
78-79	0.006249346448298413
80-81	0.00847394079766717
82-83	0.03404173776052819
84-85	0.019883399568591593
86-87	0.0147915279092606
88-89	0.006551322158033637
90-91	2.8479188548176536E-5
92-93	0.0
94-95	9.738937078772711E-6
96-97	4.9511847838604845E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	802.0
20-21	2680.0
22-23	1750.0
24-25	1842.0
26-27	3155.0
28-29	6838.0
30-31	17664.0
32-33	22870.0
34-35	31566.0
36-37	48164.0
38-39	54978.0
40-41	53042.0
42-43	61271.0
44-45	74094.0
46-47	74352.0
48-49	68949.0
50-51	80808.0
52-53	55107.0
54-55	59209.0
56-57	61028.0
58-59	64954.0
60-61	63521.0
62-63	61631.0
64-65	65958.0
66-67	69845.0
68-69	72557.0
70-71	76218.0
72-73	63479.0
74-75	63394.0
76-77	66024.0
78-79	68456.0
80-81	67992.0
82-83	62777.0
84-85	60706.0
86-87	62614.0
88-89	66496.0
90-91	67520.0
92-93	66273.0
94-95	71337.0
96-97	202653.0
98-99	1548174.0
100-101	3328622.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.2994973441835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.71790617888207	80.98645013056269
2	6.110796489325839	10.791605167601455
3	1.2810749154613532	3.3935481328643955
4	0.4262690486810482	1.5055737092767938
5	0.18301165641930695	0.8079918634975609
6	0.08757609780958733	0.4639755249570967
7	0.05481531278388293	0.33881151959067407
8	0.03365392355232117	0.237729962666363
9	0.023845245839408745	0.18949708995014478
>10	0.07918468550723223	1.062933974381389
>50	0.0012582337971425378	0.0714388695025289
>100	5.171333852118922E-4	0.07848658840838064
>500	6.830895004040391E-5	0.04378973510904092
>1k	2.2769605580063568E-5	0.02816773163150345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.4042241871999348E-5	0.0	0.0	0.0
6	0.0	1.4042241871999348E-5	0.0	0.0	0.0
7	1.4042241871999348E-5	1.4042241871999348E-5	0.0	1.4042241871999348E-5	0.0
8	1.4042241871999348E-5	1.4042241871999348E-5	0.0	1.4042241871999348E-5	0.0
9	1.4042241871999348E-5	4.212672561599804E-5	0.0	2.8084483743998696E-5	0.0
10-11	1.4042241871999348E-5	4.212672561599804E-5	0.0	6.319008842399707E-5	0.0
12-13	1.4042241871999348E-5	4.212672561599804E-5	0.0	7.021120935999674E-5	0.0
14-15	1.4042241871999348E-5	1.1233793497599478E-4	0.0	7.021120935999674E-5	0.0
16-17	2.8084483743998696E-5	1.1233793497599478E-4	0.0	7.021120935999674E-5	0.0
18-19	3.510560467999837E-5	1.2638017684799414E-4	0.0	7.021120935999674E-5	0.0
20-21	4.212672561599804E-5	1.4042241871999348E-4	0.0	7.723233029599641E-5	0.0
22-23	5.616896748799739E-5	1.4744353965599316E-4	0.0	9.127457216799576E-5	7.021120935999674E-6
24-25	5.616896748799739E-5	1.6850690246399217E-4	0.0	1.1233793497599478E-4	1.4042241871999348E-5
26-27	5.616896748799739E-5	1.6850690246399217E-4	0.0	1.9659138620799086E-4	4.9147846551997714E-5
28-29	5.616896748799739E-5	1.7552802339999184E-4	0.0	5.897741586239726E-4	5.616896748799739E-5
30-31	5.616896748799739E-5	2.2467586995198957E-4	0.0	0.001979956103951908	5.616896748799739E-5
32-33	5.616896748799739E-5	2.2467586995198957E-4	0.0	0.005350094153231752	5.616896748799739E-5
34-35	5.616896748799739E-5	2.738237165039873E-4	0.0	0.00949255550547156	8.425345123199608E-5
36-37	7.021120935999674E-5	3.159504421199853E-4	0.0	0.01658388765083123	8.425345123199608E-5
38-39	8.425345123199608E-5	3.5105604679998374E-4	0.0	0.03423498568393441	8.425345123199608E-5
40-41	8.425345123199608E-5	3.791405305439824E-4	0.0	0.05261628029438156	8.425345123199608E-5
42-43	9.829569310399544E-5	4.072250142879811E-4	0.0	0.06395539060602104	8.425345123199608E-5
44-45	9.829569310399544E-5	4.353094980319798E-4	0.0	0.07702871778885242	8.425345123199608E-5
46-47	1.0531681403999511E-4	4.4935173990397914E-4	0.0	0.09114117087021177	8.425345123199608E-5
48-49	1.1233793497599478E-4	4.4935173990397914E-4	0.0	0.10512724377472313	8.425345123199608E-5
50-51	1.5446466059199283E-4	4.914784655199772E-4	0.0	0.11939416151667445	8.425345123199608E-5
52-53	1.6850690246399217E-4	4.914784655199772E-4	0.0	0.1595058254240406	8.425345123199608E-5
54-55	1.6850690246399217E-4	4.914784655199772E-4	0.0	0.21016321297727825	8.425345123199608E-5
56-57	1.7552802339999184E-4	5.055207073919766E-4	0.0	0.279868901629883	9.127457216799576E-5
58-59	1.8254914433599154E-4	5.055207073919766E-4	0.0	0.3254078920207769	9.829569310399544E-5
60-61	1.8254914433599154E-4	5.125418283279762E-4	0.0	0.3427360184908241	9.829569310399544E-5
62-63	2.176547490159899E-4	5.195629492639759E-4	0.0	0.3552897827243915	1.1233793497599478E-4
64-65	2.2467586995198957E-4	5.616896748799739E-4	0.0	0.3619317631298472	1.1233793497599478E-4
66-67	2.3169699088798924E-4	5.897741586239726E-4	0.0	0.3683209831816069	1.1233793497599478E-4
68-69	2.457392327599886E-4	6.03816400495972E-4	0.0	0.37437318942843867	1.1233793497599478E-4
70-71	2.5978147463198795E-4	6.03816400495972E-4	0.0	0.37971626246073437	1.1233793497599478E-4
72-73	2.668025955679876E-4	6.03816400495972E-4	0.0	0.38446956133440613	1.1233793497599478E-4
74-75	2.668025955679876E-4	6.03816400495972E-4	0.0	0.38789586835117396	1.1233793497599478E-4
76-77	2.668025955679876E-4	6.24879763303971E-4	0.0	0.3913572809726218	1.1233793497599478E-4
78-79	2.668025955679876E-4	6.4594312611197E-4	0.0	0.39347063837435775	1.1233793497599478E-4
80-81	2.668025955679876E-4	6.4594312611197E-4	0.0	0.3943201940076137	1.1233793497599478E-4
82-83	2.668025955679876E-4	6.4594312611197E-4	0.0	0.3949450737709177	1.2638017684799414E-4
84-85	2.668025955679876E-4	6.4594312611197E-4	0.0	0.3952118763664857	1.2638017684799414E-4
86-87	2.8084483743998697E-4	6.4594312611197E-4	0.0	0.39529612981771767	1.2638017684799414E-4
88	2.8084483743998697E-4	6.740276098559687E-4	0.0	0.39530315093865365	1.2638017684799414E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12480	0.0	51.567535	1
GTATCAA	23045	0.0	37.28947	1
TGGTATC	5235	0.0	30.693796	2
TCAACGC	27850	0.0	30.313606	4
ATCAACG	28070	0.0	30.003374	3
TATCAAC	28755	0.0	29.52189	2
CAACGCA	28610	0.0	29.508352	5
GTGGTAT	5770	0.0	28.93282	1
AACGCAG	29405	0.0	28.738945	6
ACGCAGA	33340	0.0	25.359327	7
CGCAGAG	33915	0.0	24.941687	8
GCAGAGT	38485	0.0	22.088358	9
AAAGGCG	2610	0.0	21.134182	94
GAGTACT	21130	0.0	20.421373	12-13
TTAGCCG	675	5.456968E-12	19.770687	94
AGGACCG	1595	0.0	19.623095	5
GGACCGA	1345	0.0	19.54723	6
CAGAGTA	37955	0.0	19.488623	10-11
GTACATG	30075	0.0	19.469671	1
TACATGG	30155	0.0	18.933607	2
>>END_MODULE