FastQCFastQC Report
Fri 27 May 2016
SRR2139609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7316053
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107000.053.7428441
GTATCAA213150.040.337871
TCAACGC257750.032.6937564
ATCAACG262800.031.8879683
CAACGCA265450.031.7614545
TATCAAC269700.031.3109322
AACGCAG273700.030.8508076
CGAGACC54350.027.91481886-87
ACGCAGA313450.026.9926877
GAGACCG56150.026.81057488-89
CGCAGAG321300.026.306678
AGACCGA57900.026.24053688-89
ACCGAGG59300.026.11736390-91
GACCGAG59900.025.28956890-91
TGGTATC48700.025.2090232
ATCTCCG57900.024.35830174-75
GCCCACG60600.024.09842982-83
GTGGTAT53700.023.973151
CACGAGA62450.023.94162284-85
CCCACGA62850.023.45006882-83
Produced by FastQC (version 0.11.5)