##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7316053
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.524606779092498	32.0	25.0	33.0	18.0	33.0
2	31.150137375986752	33.0	31.0	33.0	27.0	33.0
3	30.596637558530535	31.0	30.0	33.0	27.0	33.0
4	31.751890397732218	33.0	31.0	33.0	29.0	33.0
5	32.10057103194851	33.0	32.0	33.0	31.0	33.0
6	35.17460671758392	37.0	35.0	38.0	29.0	38.0
7	36.55829707630603	38.0	37.0	38.0	34.0	38.0
8	36.142000474846206	38.0	36.0	38.0	33.0	38.0
9	36.73629879389884	38.0	38.0	38.0	34.0	38.0
10-11	37.03003853307241	38.0	38.0	38.0	35.5	38.0
12-13	37.16896166553195	38.0	38.0	38.0	36.0	38.0
14-15	37.197357304546586	38.0	38.0	38.0	36.0	38.0
16-17	37.270122223007405	38.0	38.0	38.0	36.5	38.0
18-19	37.317364226311646	38.0	38.0	38.0	37.0	38.0
20-21	37.35830076197561	38.0	38.0	38.0	37.0	38.0
22-23	37.38042319082723	38.0	38.0	38.0	37.0	38.0
24-25	37.40369308996664	38.0	38.0	38.0	37.0	38.0
26-27	37.38135210190637	38.0	38.0	38.0	37.0	38.0
28-29	37.366133628126136	38.0	38.0	38.0	37.0	38.0
30-31	37.37015772350252	38.0	38.0	38.0	37.0	38.0
32-33	37.359124754625576	38.0	38.0	38.0	37.0	38.0
34-35	37.338522194477775	38.0	38.0	38.0	37.0	38.0
36-37	37.320618824467786	38.0	38.0	38.0	37.0	38.0
38-39	37.31071001175583	38.0	38.0	38.0	37.0	38.0
40-41	37.30187318822864	38.0	38.0	38.0	37.0	38.0
42-43	37.2586198202212	38.0	38.0	38.0	37.0	38.0
44-45	37.21546616778258	38.0	38.0	38.0	37.0	38.0
46-47	37.18144247821105	38.0	38.0	38.0	37.0	38.0
48-49	37.124644939638195	38.0	38.0	38.0	36.5	38.0
50-51	37.088768223697045	38.0	38.0	38.0	36.0	38.0
52-53	37.07894008212352	38.0	38.0	38.0	36.0	38.0
54-55	37.0563781473315	38.0	38.0	38.0	36.0	38.0
56-57	37.00149565154259	38.0	38.0	38.0	36.0	38.0
58-59	36.97906641880559	38.0	38.0	38.0	36.0	38.0
60-61	37.006904318813554	38.0	38.0	38.0	36.0	38.0
62-63	37.01316126554906	38.0	38.0	38.0	36.0	38.0
64-65	37.01981128675965	38.0	38.0	38.0	36.0	38.0
66-67	37.01701296397407	38.0	38.0	38.0	36.0	38.0
68-69	37.007553553450784	38.0	38.0	38.0	36.0	38.0
70-71	37.000669721703815	38.0	38.0	38.0	36.0	38.0
72-73	36.994263046960015	38.0	38.0	38.0	36.0	38.0
74-75	36.969800659802345	38.0	38.0	38.0	36.0	38.0
76-77	36.94516793662709	38.0	38.0	38.0	36.0	38.0
78-79	36.946121303567054	38.0	38.0	38.0	36.0	38.0
80-81	36.938016522355866	38.0	38.0	38.0	35.5	38.0
82-83	36.886671642317566	38.0	38.0	38.0	35.0	38.0
84-85	36.867011661192294	38.0	38.0	38.0	35.0	38.0
86-87	36.86609535467949	38.0	38.0	38.0	35.0	38.0
88-89	36.84713446018851	38.0	38.0	38.0	35.0	38.0
90-91	36.81813411733066	38.0	38.0	38.0	35.0	38.0
92-93	36.807220177521884	38.0	38.0	38.0	35.0	38.0
94-95	36.778260669784615	38.0	38.0	38.0	35.0	38.0
96-97	36.74472097948155	38.0	38.0	38.0	34.5	38.0
98-99	36.7228939780512	38.0	38.0	38.0	34.5	38.0
100	35.29079930344625	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	21.0
21	97.0
22	403.0
23	1353.0
24	3241.0
25	6676.0
26	12105.0
27	20311.0
28	32604.0
29	47705.0
30	64490.0
31	84336.0
32	111372.0
33	151834.0
34	230251.0
35	423380.0
36	1230400.0
37	4895471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.518220275331515	19.427141930218383	14.361145278745246	24.693492515704847
2	14.638702266817413	20.52588534590594	42.47892889524791	22.356483492028733
3	18.147595559434563	25.799089223434144	30.422903508670014	25.63041170846128
4	12.519838224244683	17.541029295441135	39.43599096398017	30.503141516334015
5	13.363577340231409	37.166435439889014	35.613425277301275	13.856561942578304
6	29.977761162816446	37.82610240799997	18.553887832608666	13.642248596574918
7	26.6898558553362	32.80442336872081	23.052047326611767	17.453673449331216
8	24.492263793058907	36.44895683505847	21.03739543712983	18.021383934752798
9	25.90403596037372	16.776436693391915	21.547356204226514	35.772171142007856
10-11	24.1520120206893	26.717814920148886	28.867512304790576	20.262660754371243
12-13	25.071018484967233	24.57435040451457	29.03214342487677	21.322487685641427
14-15	22.362953084128833	25.75290938980349	26.91344636240333	24.97069116366434
16-17	21.455031832054797	28.92139381713063	27.672052129748103	21.95152222106647
18-19	21.463834392670474	27.85145214229585	29.80193691871833	20.882776546315345
20-21	22.30177766128925	26.987783170498087	29.773162887984544	20.93727628022812
22-23	22.12135966988499	26.764555476712427	29.63954944500801	21.474535408394573
24-25	21.821614212723304	27.02321376475468	29.747615723633107	21.40755629888891
26-27	21.64119137173904	27.115673667741046	29.77768258335365	21.465452377166265
28-29	21.58180166199833	26.999563315940062	29.900492799842425	21.518142222219183
30-31	21.8614500946583	26.96810369525059	29.74091590078044	21.429530309310675
32-33	21.587671870153326	27.211372078860762	29.656567685317537	21.544388365668375
34-35	21.818518592139043	27.17475268795609	29.659926896040663	21.346801823864205
36-37	21.818751570530573	26.982348807322076	29.58584396679062	21.613055655356728
38-39	21.576018073464773	27.273868133989765	29.666247887875624	21.483865904669834
40-41	21.787331456832074	27.057318882600878	29.67384369107467	21.481505969492375
42-43	21.76039928177116	27.152630662603034	29.33475197011718	21.752218085508627
44-45	21.740752046943985	27.276848119570708	29.285771912016607	21.696627921468696
46-47	21.924244468863975	27.33505332188709	28.969684021149877	21.77101818809905
48-49	21.848833978234723	27.49453499509536	28.802814419756285	21.853816606913636
50-51	21.80777190650956	27.60006067648749	28.65115046829475	21.941016948708196
52-53	21.9767481874441	27.53070869169098	28.568704330242685	21.923838790622234
54-55	22.00099221997245	27.489475032555372	28.61850221202079	21.89103053545139
56-57	21.940710582909663	27.57255388080561	28.549624384799046	21.937111151485684
58-59	21.799717308221577	27.446164154013385	28.604565840532466	22.149552697232576
60-61	22.044791615167846	27.544982766214154	28.687586730305686	21.72263888831231
62-63	21.790812654594973	27.519415827511995	28.783531500507014	21.906240017386015
64-65	21.757272288264765	27.346400258165136	28.778400428802108	22.117927024767987
66-67	21.801129616193222	27.48210992048191	28.72619258746173	21.990567875863135
68-69	21.75110554723318	27.665820363324357	28.694997120380595	21.88807696906187
70-71	21.815166632921514	27.392711331568908	28.62185016111665	22.17027187439292
72-73	21.899694931231874	27.532661444404273	28.392481082860698	22.175162541503155
74-75	21.748846226569203	27.68368889792128	28.58493772581867	21.98252714969085
76-77	21.92220483116443	27.363141195152018	28.54790565453957	22.166748319143984
78-79	21.892649497661562	27.350976099570488	28.224532613740994	22.531841789026956
80-81	22.094588455848417	27.77622490442789	28.08349505991648	22.045691579807215
82-83	22.25455485918605	27.426680326222332	28.181203380070443	22.13756143452118
84-85	21.99562408583343	27.429607219911873	28.110258027488527	22.464510666766166
86-87	21.914245679844097	27.792045679741683	28.186871887762916	22.106836752651308
88-89	22.245586968766027	27.71334292871651	28.11336038113883	21.92770972137863
90-91	22.26796575191714	27.74533163937963	28.10673651683312	21.879966091870113
92-93	22.138793685160255	27.507012018626803	28.05370580128159	22.30048849493135
94-95	22.188109693484623	27.678185594975268	28.116995544602464	22.01670916693764
96-97	22.59354231928934	27.581967959084817	28.09904932045742	21.72544040116842
98-99	23.1819974373821	29.638989116486158	28.46734528403167	18.71166816210007
100	29.578377087263014	40.443520842012845	29.978102070724145	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2249.0
1	1963.5
2	2285.0
3	3134.5
4	3853.0
5	5207.5
6	6697.5
7	9197.0
8	12445.5
9	13984.5
10	13124.0
11	12061.5
12	12939.0
13	16013.0
14	22280.5
15	29442.5
16	34555.0
17	37093.0
18	36194.0
19	32583.5
20	28263.5
21	24883.0
22	23942.5
23	26202.0
24	31413.0
25	39714.5
26	51299.5
27	64839.5
28	78066.5
29	93990.0
30	112116.0
31	129759.5
32	153385.0
33	180550.5
34	201049.5
35	221993.0
36	248218.5
37	265217.5
38	278184.5
39	287969.0
40	293757.5
41	296258.0
42	294498.0
43	299261.0
44	304076.5
45	303866.5
46	299088.0
47	293810.0
48	291635.5
49	288045.0
50	281649.5
51	273135.0
52	262090.0
53	248949.0
54	233384.5
55	216772.5
56	198455.5
57	178366.0
58	158756.5
59	137511.0
60	115338.0
61	93928.0
62	74177.5
63	57526.5
64	43955.5
65	33021.5
66	24007.5
67	17989.0
68	13669.0
69	10163.5
70	7524.0
71	5251.5
72	3645.0
73	2526.5
74	1675.0
75	1096.5
76	729.5
77	449.5
78	291.5
79	182.0
80	109.0
81	73.0
82	55.5
83	41.5
84	31.5
85	26.0
86	23.5
87	19.5
88	12.0
89	7.5
90	3.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006369554731219142
3	0.004346606018299759
4	0.0
5	4.10057171537713E-5
6	3.1437716484557996E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.212655291725164E-4
26-27	0.0019210187825731467
28-29	0.00677720555640584
30-31	0.0025328283649394944
32-33	0.005473588904664897
34-35	0.002668609157359835
36-37	0.0012429436191797524
38-39	3.4724597481147147E-5
40-41	1.5376169244097035E-4
42-43	0.002194735350440498
44-45	4.6080574223505104E-4
46-47	2.933578838106731E-4
48-49	0.0018485932782837662
50-51	5.024791886228226E-4
52-53	0.0
54-55	0.004767218212872627
56-57	0.04702386179796514
58-59	0.07067172242833974
60-61	0.03228711705547529
62-63	0.01351251208403565
64-65	0.001680254864740634
66-67	0.0
68-69	0.002211436282542789
70-71	0.0038188688415808536
72-73	0.012966748176112972
74-75	0.015697922143289637
76-77	0.0206523497058724
78-79	0.006163432763218535
80-81	0.008148815254184653
82-83	0.032439288035860096
84-85	0.018428888656051537
86-87	0.012996458529051779
88-89	0.005733792186160453
90-91	4.358369551384305E-5
92-93	0.0
94-95	0.0
96-97	3.622221495765578E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	68.0
20-21	312.0
22-23	722.0
24-25	808.0
26-27	1890.0
28-29	4903.0
30-31	13751.0
32-33	18278.0
34-35	25407.0
36-37	39229.0
38-39	45584.0
40-41	45109.0
42-43	51746.0
44-45	63538.0
46-47	65223.0
48-49	59782.0
50-51	53437.0
52-53	48494.0
54-55	52178.0
56-57	54032.0
58-59	31072.0
60-61	55542.0
62-63	53911.0
64-65	57075.0
66-67	59870.0
68-69	61736.0
70-71	60346.0
72-73	57063.0
74-75	58573.0
76-77	60284.0
78-79	62541.0
80-81	62639.0
82-83	59247.0
84-85	57629.0
86-87	59703.0
88-89	62242.0
90-91	62924.0
92-93	62907.0
94-95	72975.0
96-97	242480.0
98-99	1693566.0
100-101	3617237.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.44438114280454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.67604428820711	78.384345329716
2	6.671543320664979	11.534348672445907
3	1.5146963132794988	3.928109562622016
4	0.5303842188451843	1.8339494226392707
5	0.24020630280434432	1.038224259626133
6	0.11841897187386256	0.6141992843521937
7	0.07148635729295627	0.43257157414401115
8	0.04204581595488936	0.29076996318917353
9	0.028191999275163145	0.2193335937467885
>10	0.1039137575930486	1.3624889979988157
>50	0.002196467441953949	0.12664228939898198
>100	7.474524298616369E-4	0.14204968343933835
>500	9.978751209452993E-5	0.0658815652304041
>1k	2.4946825066838026E-5	0.027085801451020554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.834286192295218E-5	0.0	0.0	0.0
3	0.0	8.20114343075426E-5	0.0	0.0	0.0
4	0.0	1.3668572384590435E-4	0.0	1.3668572384590434E-5	0.0
5	0.0	1.3668572384590435E-4	0.0	1.3668572384590434E-5	0.0
6	1.3668572384590434E-5	1.3668572384590435E-4	0.0	1.3668572384590434E-5	0.0
7	1.3668572384590434E-5	1.3668572384590435E-4	0.0	1.3668572384590434E-5	0.0
8	1.3668572384590434E-5	1.640228686150852E-4	0.0	1.3668572384590434E-5	0.0
9	1.3668572384590434E-5	2.870400200763991E-4	0.0	2.7337144769180868E-5	0.0
10-11	1.3668572384590434E-5	2.870400200763991E-4	0.0	2.7337144769180868E-5	0.0
12-13	4.10057171537713E-5	2.870400200763991E-4	0.0	2.7337144769180868E-5	0.0
14-15	4.10057171537713E-5	3.0070859246098956E-4	0.0	2.7337144769180868E-5	1.3668572384590434E-5
16-17	4.784000334606652E-5	3.0070859246098956E-4	0.0	2.7337144769180868E-5	2.7337144769180868E-5
18-19	6.150857573065696E-5	3.1437716484558E-4	0.0	2.7337144769180868E-5	2.7337144769180868E-5
20-21	6.834286192295218E-5	3.417143096147608E-4	0.0	4.10057171537713E-5	3.417143096147608E-5
22-23	8.20114343075426E-5	3.622171681916465E-4	0.0	4.10057171537713E-5	4.10057171537713E-5
24-25	9.568000669213305E-5	4.1689145773000826E-4	0.0	6.834286192295218E-5	4.10057171537713E-5
26-27	1.1618286526901869E-4	4.373943163068939E-4	0.0	8.884572049983782E-5	4.10057171537713E-5
28-29	1.230171514613139E-4	4.373943163068939E-4	0.0	4.3056003011459867E-4	4.10057171537713E-5
30-31	1.3668572384590435E-4	4.784000334606652E-4	0.0	0.0016197258275739664	4.10057171537713E-5
32-33	1.3668572384590435E-4	4.784000334606652E-4	0.0	0.0038613716986467977	4.10057171537713E-5
34-35	1.5035429623049478E-4	5.67245753960503E-4	0.0	0.007039314778064074	4.10057171537713E-5
36-37	1.640228686150852E-4	6.629257606526361E-4	0.0	0.012677600886707628	4.10057171537713E-5
38-39	1.640228686150852E-4	6.697600468449313E-4	0.0	0.02750800192398825	4.10057171537713E-5
40-41	1.640228686150852E-4	7.24434336383293E-4	0.0	0.04293982014619085	4.10057171537713E-5
42-43	1.708571548073804E-4	7.449371949601786E-4	0.0	0.052002083637174304	4.10057171537713E-5
44-45	1.9819429957656132E-4	8.747886326137878E-4	0.0	0.06336750157496125	4.10057171537713E-5
46-47	2.1186287196115172E-4	9.841372116905112E-4	0.0	0.07498578810186313	5.4674289538361737E-5
48-49	2.3236573053803737E-4	9.909714978828064E-4	0.0	0.08612567459530432	5.4674289538361737E-5
50-51	2.3236573053803737E-4	0.001161828652690187	0.0	0.09852306974812786	5.4674289538361737E-5
52-53	2.460343029226278E-4	0.001161828652690187	0.0	0.1347516208534848	6.150857573065696E-5
54-55	2.460343029226278E-4	0.0011754972250747773	0.0	0.1806438526347472	6.834286192295218E-5
56-57	2.460343029226278E-4	0.0012165029422285486	0.0	0.23412897637564953	6.834286192295218E-5
58-59	2.460343029226278E-4	0.0012165029422285486	0.0	0.44113267085407937	6.834286192295218E-5
60-61	2.460343029226278E-4	0.001223337228420844	0.0	0.6278248667690078	6.834286192295218E-5
62-63	2.460343029226278E-4	0.0012780115179592056	0.0	0.6344131186583803	6.834286192295218E-5
64-65	2.460343029226278E-4	0.0013805258108436338	0.0	0.640700661955292	6.834286192295218E-5
66-67	2.733714476918087E-4	0.0014078629556128147	0.0	0.6468105138112039	6.834286192295218E-5
68-69	2.733714476918087E-4	0.0014625372451511766	0.0	0.6525239770679627	6.834286192295218E-5
70-71	2.733714476918087E-4	0.001510377248497243	0.0	0.6576565259983764	6.834286192295218E-5
72-73	2.733714476918087E-4	0.001537714393266424	0.0	0.6614495548351003	1.0934857907672347E-4
74-75	2.733714476918087E-4	0.0015718858242278998	0.0	0.6645523207664024	1.0934857907672347E-4
76-77	2.733714476918087E-4	0.0016470629723431473	0.0	0.667832778138704	1.0934857907672347E-4
78-79	2.733714476918087E-4	0.0016949029756892138	0.0	0.6699650754307002	1.0934857907672347E-4
80-81	2.870400200763991E-4	0.0016949029756892138	0.0	0.6708261954909294	1.0934857907672347E-4
82-83	2.870400200763991E-4	0.0017085715480738043	0.0	0.6715232926825434	1.0934857907672347E-4
84-85	2.870400200763991E-4	0.0017222401204583947	0.0	0.6718308355611967	1.0934857907672347E-4
86-87	2.870400200763991E-4	0.0017359086928429852	0.0	0.6719470184264658	1.0934857907672347E-4
88	2.870400200763991E-4	0.001831588699535118	0.0	0.6719470184264658	1.0934857907672347E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10700	0.0	53.742844	1
GTATCAA	21315	0.0	40.33787	1
TCAACGC	25775	0.0	32.693756	4
ATCAACG	26280	0.0	31.887968	3
CAACGCA	26545	0.0	31.761454	5
TATCAAC	26970	0.0	31.310932	2
AACGCAG	27370	0.0	30.850807	6
CGAGACC	5435	0.0	27.914818	86-87
ACGCAGA	31345	0.0	26.992687	7
GAGACCG	5615	0.0	26.810574	88-89
CGCAGAG	32130	0.0	26.30667	8
AGACCGA	5790	0.0	26.240536	88-89
ACCGAGG	5930	0.0	26.117363	90-91
GACCGAG	5990	0.0	25.289568	90-91
TGGTATC	4870	0.0	25.209023	2
ATCTCCG	5790	0.0	24.358301	74-75
GCCCACG	6060	0.0	24.098429	82-83
GTGGTAT	5370	0.0	23.97315	1
CACGAGA	6245	0.0	23.941622	84-85
CCCACGA	6285	0.0	23.450068	82-83
>>END_MODULE