FastQCFastQC Report
Fri 27 May 2016
SRR2139608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6026038
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT64250.062.7428994
GGTATCA98900.055.4924051
TATCTCG80400.047.2362794
GTATCAA183650.038.5750051
GCCGTCT40900.032.86625390-91
TATGCCG38400.032.708886-87
CCGTCTT39900.032.54793590-91
TCTCGTA38650.032.3390982-83
TGCCGTC39700.032.1624388-89
ATGCCGT41700.031.62709888-89
TCAACGC227800.030.7012774
ATCAACG228000.030.5643353
TATCAAC230600.030.4250562
CTCGTAT41300.030.19869682-83
CAACGCA233800.029.8403875
TCGTATG44400.029.3506184-85
AACGCAG240700.028.9672436
CTATCTC101050.027.390592-93
CGTATGC47000.026.79691384-85
TGGTATC41350.026.6276632
Produced by FastQC (version 0.11.5)