##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139608_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6026038
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.801776225108437	32.0	25.0	33.0	18.0	33.0
2	30.862074052636242	32.0	30.0	33.0	27.0	33.0
3	30.276361018632805	31.0	29.0	33.0	25.0	33.0
4	31.587812257406938	33.0	31.0	33.0	29.0	33.0
5	31.93302199554666	33.0	32.0	33.0	31.0	33.0
6	34.78170233908249	37.0	34.0	38.0	29.0	38.0
7	36.297964101786285	38.0	37.0	38.0	33.0	38.0
8	35.81483638171548	38.0	36.0	38.0	31.0	38.0
9	36.53330911620537	38.0	37.0	38.0	34.0	38.0
10-11	36.87931191273603	38.0	38.0	38.0	35.0	38.0
12-13	37.04198455104332	38.0	38.0	38.0	35.5	38.0
14-15	37.09588464261261	38.0	38.0	38.0	36.0	38.0
16-17	37.17722722624716	38.0	38.0	38.0	36.0	38.0
18-19	37.25390878716662	38.0	38.0	38.0	36.0	38.0
20-21	37.30706066180342	38.0	38.0	38.0	37.0	38.0
22-23	37.34152210857074	38.0	38.0	38.0	37.0	38.0
24-25	37.37094207122276	38.0	38.0	38.0	37.0	38.0
26-27	37.35583656234106	38.0	38.0	38.0	37.0	38.0
28-29	37.33757844390665	38.0	38.0	38.0	37.0	38.0
30-31	37.342942263240346	38.0	38.0	38.0	37.0	38.0
32-33	37.32966418615865	38.0	38.0	38.0	37.0	38.0
34-35	37.305168448601464	38.0	38.0	38.0	37.0	38.0
36-37	37.286070924054386	38.0	38.0	38.0	37.0	38.0
38-39	37.274494583443804	38.0	38.0	38.0	37.0	38.0
40-41	37.265049811974436	38.0	38.0	38.0	37.0	38.0
42-43	37.22109009087592	38.0	38.0	38.0	37.0	38.0
44-45	37.175074791951474	38.0	38.0	38.0	36.0	38.0
46-47	37.13671448131642	38.0	38.0	38.0	36.0	38.0
48-49	37.07746582620703	38.0	38.0	38.0	36.0	38.0
50-51	37.03209258132732	38.0	38.0	38.0	36.0	38.0
52-53	37.014300324824376	38.0	38.0	38.0	36.0	38.0
54-55	36.987199303544664	38.0	38.0	38.0	36.0	38.0
56-57	36.932359471598375	38.0	38.0	38.0	36.0	38.0
58-59	36.90686670887044	38.0	38.0	38.0	36.0	38.0
60-61	36.928980095288225	38.0	38.0	38.0	36.0	38.0
62-63	36.93563376759262	38.0	38.0	38.0	36.0	38.0
64-65	36.93815805517913	38.0	38.0	38.0	36.0	38.0
66-67	36.933358947952414	38.0	38.0	38.0	36.0	38.0
68-69	36.91715251732196	38.0	38.0	38.0	35.5	38.0
70-71	36.90462470117402	38.0	38.0	38.0	35.0	38.0
72-73	36.89833312414521	38.0	38.0	38.0	35.0	38.0
74-75	36.86478689024277	38.0	38.0	38.0	35.0	38.0
76-77	36.82950500696998	38.0	38.0	38.0	35.0	38.0
78-79	36.82375937456506	38.0	38.0	38.0	35.0	38.0
80-81	36.80773398017972	38.0	38.0	38.0	35.0	38.0
82-83	36.72076290691184	38.0	38.0	38.0	34.5	38.0
84-85	36.729723824766246	38.0	38.0	38.0	34.5	38.0
86-87	36.73098985243654	38.0	38.0	38.0	35.0	38.0
88-89	36.70530666094507	38.0	38.0	38.0	34.0	38.0
90-91	36.683620755303394	38.0	38.0	38.0	34.0	38.0
92-93	36.66995494210842	38.0	38.0	38.0	34.0	38.0
94-95	36.60320065596998	38.0	38.0	38.0	34.0	38.0
96-97	36.500786942812695	38.0	38.0	38.0	34.0	38.0
98-99	36.5082124887216	38.0	38.0	38.0	34.0	38.0
100	35.03760109418978	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	15.0
21	93.0
22	389.0
23	1212.0
24	2856.0
25	5723.0
26	10594.0
27	17925.0
28	28452.0
29	41815.0
30	56599.0
31	74150.0
32	98133.0
33	135746.0
34	210628.0
35	397850.0
36	1184717.0
37	3759138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.195369494848855	18.25624066758291	14.08383086864039	25.464558968927843
2	14.791910934311813	20.044791736679684	42.278088808455166	22.88520852055333
3	18.662949965091617	25.08020132852665	29.98083399424439	26.276014712137346
4	12.797463275206693	17.070768554728662	38.556344981561686	31.57542318850296
5	13.811353568308181	36.66452318979229	35.03155889403231	14.492564347867212
6	30.650804561154647	36.8076563993005	18.359321196850058	14.182217842694794
7	27.230146905811083	32.50578572521447	22.31044676452422	17.953620604450222
8	24.835737843007298	36.00825285203976	20.708896956839634	18.447112348113304
9	26.07476089596514	16.71590852895385	21.048705633784586	36.16062494129642
10-11	24.63127514297122	26.371124775515852	28.265147016995247	20.73245306451768
12-13	25.56854105466975	24.110062034125903	28.516937662855764	21.804459248348586
14-15	22.778357853037104	25.236606539819363	26.513191586246222	25.47184402089731
16-17	21.96289668269599	28.42018254780338	27.162241592236892	22.454679177263735
18-19	21.920206941940958	27.358506534475886	29.315837371088598	21.40544915249456
20-21	23.001945257964902	26.408602746527677	29.169119950825895	21.420332044681523
22-23	22.726684331221353	26.231697752233547	29.095640851257613	21.94597706528749
24-25	22.28530404128346	26.4263388779995	29.240614013707432	22.047743067009606
26-27	22.12171987093755	26.559812400775545	29.23756855083467	22.08089917745224
28-29	22.127378703923544	26.55811454783916	29.288011739978835	22.02649500825846
30-31	22.38093895863407	26.513516351751164	29.102093585890998	22.003451103723766
32-33	22.120864699057854	26.692932165504825	29.062620798383687	22.123582337053634
34-35	22.29886540046493	26.664690918845697	29.07111328705466	21.965330393634712
36-37	22.30362528685642	26.483514534493004	29.039792862365825	22.173067316284754
38-39	22.060785070637156	26.627794878104677	29.112813594568337	22.198606456689827
40-41	22.47380807854733	26.464825246792838	29.058214574210155	22.00315210044968
42-43	22.15761929189185	26.578239585661116	28.841838442063057	22.422302680383975
44-45	22.220925687651683	26.685689421885606	28.76202038809256	22.331364502370153
46-47	22.343937214516632	26.623410717335595	28.62394450052614	22.40870756762164
48-49	22.271548676043057	26.942111038816	28.341506996979792	22.44483328816115
50-51	22.2167757957599	27.11931729801981	28.050415546293955	22.613491359926336
52-53	22.29816777273855	26.912161724175697	27.899744958972146	22.889925544113606
54-55	22.493761653832014	26.622506943527174	28.284407374117137	22.599324028523675
56-57	22.26249000722365	26.382658866103696	28.391339074143403	22.963512052529254
58-59	22.0482189316756	26.104451780664732	28.468545728809158	23.378783558850508
60-61	22.27008839012691	26.38718701749575	28.853815133501087	22.488909458876254
62-63	21.759147539674416	26.613658353098714	28.834269584816326	22.79292452241055
64-65	21.5169193921046	26.947167244840053	28.54541550093516	22.990497862120186
66-67	21.61252483354705	27.70084309770267	27.866122846175628	22.820509222574653
68-69	21.786388899115725	27.684719829351256	27.739869609090245	22.789021662442774
70-71	21.756418530733374	27.04039567396816	27.813239121990136	23.38994667330833
72-73	22.094293325983138	26.46638662599618	27.731185909121752	23.70813413889893
74-75	22.084059846771712	26.580595122968003	27.9056780751696	23.429666955090685
76-77	22.508681332915035	26.51278386405682	27.12796757950075	23.850567223527396
78-79	22.780688394398833	26.595594836883524	26.49769087706258	24.126025891655065
80-81	22.53615183646069	27.073581167229015	26.697406686555496	23.692860309754796
82-83	22.561130678726116	26.742995062433412	26.776210848172234	23.91966341066824
84-85	22.615360692886323	27.201967078639512	26.686739817506012	23.49593241096816
86-87	23.18139865442873	27.090830098899843	26.782828093526916	22.944943153144514
88-89	22.907981150748462	27.37320678227075	27.082055146867486	22.636756920113307
90-91	22.871597315780477	26.850991014839764	26.908576919200744	23.368834750179015
92-93	22.834991880411696	26.363191399919145	27.202975333807384	23.59884138586178
94-95	22.022957058443485	26.877957994054448	27.877492414926465	23.221592532575606
96-97	22.22139234277105	26.672059164414026	28.119050970483865	22.98749752233106
98-99	23.43459790841639	28.552413767111773	28.309990974223386	19.70299735024845
100	30.42453545570264	39.85404294827387	29.721421596023482	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1943.0
1	1729.0
2	1824.5
3	2338.5
4	2986.5
5	4170.0
6	5356.0
7	6992.0
8	8973.0
9	9981.0
10	9705.5
11	9280.5
12	10189.0
13	12727.5
14	17811.5
15	23933.5
16	28581.5
17	31062.0
18	30577.0
19	27613.5
20	23826.0
21	20340.0
22	18697.0
23	19932.5
24	23689.5
25	29719.5
26	38666.0
27	49279.5
28	59559.5
29	71348.0
30	83172.5
31	94155.5
32	108438.0
33	124897.5
34	139314.0
35	153596.0
36	170641.0
37	183912.5
38	193564.0
39	202779.0
40	212651.0
41	220614.5
42	225202.0
43	232342.0
44	239996.5
45	245409.0
46	248517.0
47	250599.5
48	253199.5
49	254341.5
50	253415.5
51	251090.5
52	244488.5
53	233359.0
54	220927.0
55	207859.5
56	191753.5
57	173933.5
58	155815.0
59	134727.0
60	113120.5
61	92146.5
62	72967.0
63	56853.0
64	42749.0
65	31678.0
66	23278.0
67	17734.5
68	13713.0
69	10263.5
70	7544.5
71	5315.5
72	3628.5
73	2434.5
74	1597.5
75	1056.0
76	693.5
77	416.0
78	263.5
79	172.0
80	110.0
81	66.5
82	43.5
83	31.5
84	27.5
85	23.5
86	15.5
87	8.0
88	4.0
89	3.5
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006189804976337686
3	0.004397582623939644
4	0.0
5	4.978395423327898E-5
6	3.3189302822185984E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.4024896265560163E-4
26-27	0.002099922443971081
28-29	0.007747546568733691
30-31	0.002652322286923735
32-33	0.006251843772926032
34-35	0.00306975948978088
36-37	0.0011263566187951094
38-39	8.450633049597694E-6
40-41	1.1900478339726865E-4
42-43	0.0023982127833135094
44-45	5.877588840187496E-4
46-47	3.494476761074325E-4
48-49	0.0020569841099301748
50-51	5.704898609243772E-4
52-53	0.0
54-55	0.005154356881227511
56-57	0.04778836417814313
58-59	0.07061470915415427
60-61	0.03256054853417936
62-63	0.013208093744832753
64-65	0.0015916996085615037
66-67	0.0
68-69	0.002385509177542178
70-71	0.003589103822613956
72-73	0.012532406971587997
74-75	0.016187903758550143
76-77	0.020806178690593903
78-79	0.006150811357388982
80-81	0.00785472053174855
82-83	0.03337695439850807
84-85	0.019192623478006526
86-87	0.013893208229582885
88-89	0.006324430510686246
90-91	2.1194660344440784E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	57.0
20-21	225.0
22-23	616.0
24-25	788.0
26-27	2053.0
28-29	5271.0
30-31	14521.0
32-33	19018.0
34-35	26070.0
36-37	39606.0
38-39	24676.0
40-41	43866.0
42-43	49874.0
44-45	60415.0
46-47	60930.0
48-49	56120.0
50-51	28448.0
52-53	4780.0
54-55	4714.0
56-57	4138.0
58-59	28814.0
60-61	53157.0
62-63	50523.0
64-65	52711.0
66-67	56567.0
68-69	57787.0
70-71	56569.0
72-73	52461.0
74-75	53431.0
76-77	55459.0
78-79	57280.0
80-81	57043.0
82-83	52936.0
84-85	52160.0
86-87	53969.0
88-89	56336.0
90-91	57365.0
92-93	56313.0
94-95	65365.0
96-97	211068.0
98-99	1474297.0
100-101	2868241.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.54218827963405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.77838336606277	78.56159944986605
2	6.640744648818539	11.494091474300527
3	1.4636819729349653	3.8001072264973206
4	0.5165975064284387	1.7882991466447764
5	0.23372330213000883	1.011346300913651
6	0.12249953499972417	0.636082669326825
7	0.06630546118252564	0.4016753793938271
8	0.046095462635280804	0.31913617649754467
9	0.03080136462955278	0.23990557473363758
>10	0.09739467362909295	1.3133183956266687
>50	0.002606001088579933	0.15557198232134584
>100	0.0010492458496580855	0.1895140164946047
>500	8.809471196403062E-5	0.05415516628709574
>1k	2.9364898863106768E-5	0.03519704109619405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.3189302822185984E-5	0.0	1.6594651411092992E-5	0.0
3	0.0	4.978395423327898E-5	0.0	1.6594651411092992E-5	0.0
4	0.0	6.637860564437197E-5	0.0	1.6594651411092992E-5	0.0
5	0.0	6.637860564437197E-5	0.0	1.6594651411092992E-5	0.0
6	4.978395423327898E-5	6.637860564437197E-5	0.0	1.6594651411092992E-5	3.3189302822185984E-5
7	4.978395423327898E-5	6.637860564437197E-5	0.0	1.6594651411092992E-5	3.3189302822185984E-5
8	4.978395423327898E-5	9.956790846655797E-5	0.0	1.6594651411092992E-5	3.3189302822185984E-5
9	4.978395423327898E-5	1.6594651411092992E-4	0.0	6.637860564437197E-5	3.3189302822185984E-5
10-11	5.808127993882547E-5	1.6594651411092992E-4	0.0	8.297325705546496E-5	3.3189302822185984E-5
12-13	6.637860564437197E-5	1.6594651411092992E-4	0.0	8.297325705546496E-5	3.3189302822185984E-5
14-15	6.637860564437197E-5	2.821090739885809E-4	0.0	8.297325705546496E-5	3.3189302822185984E-5
16-17	6.637860564437197E-5	2.821090739885809E-4	0.0	8.297325705546496E-5	5.808127993882547E-5
18-19	6.637860564437197E-5	2.904063996941274E-4	0.0	8.297325705546496E-5	8.297325705546496E-5
20-21	6.637860564437197E-5	3.0700105110522037E-4	0.0	8.297325705546496E-5	8.297325705546496E-5
22-23	8.297325705546496E-5	3.650823310440459E-4	0.0	1.2445988558319745E-4	8.297325705546496E-5
24-25	8.297325705546496E-5	4.2316361098287134E-4	0.0	1.6594651411092992E-4	1.1616255987765094E-4
26-27	8.297325705546496E-5	4.3146093668841785E-4	0.0	2.2402779404975543E-4	1.3275721128874394E-4
28-29	8.297325705546496E-5	4.3146093668841785E-4	0.0	6.720833821492662E-4	1.3275721128874394E-4
30-31	8.297325705546496E-5	5.144341937438828E-4	0.0	0.0025638736430138673	1.3275721128874394E-4
32-33	8.297325705546496E-5	5.310288451549757E-4	0.0	0.006397238118976349	1.4105453699429045E-4
34-35	8.297325705546496E-5	6.720833821492662E-4	0.0	0.011964743667398048	1.4935186269983693E-4
36-37	8.297325705546496E-5	8.214352448491031E-4	0.0	0.023888000706268363	1.4935186269983693E-4
38-39	8.297325705546496E-5	8.463272219657427E-4	0.0	0.21586322555549767	1.4935186269983693E-4
40-41	9.127058276101147E-5	9.127058276101146E-4	0.0	0.41514009702560783	1.4935186269983693E-4
42-43	9.956790846655797E-5	9.375978047267541E-4	0.0	0.4430357060476552	1.4935186269983693E-4
44-45	9.956790846655797E-5	0.0010703550160154982	0.0	0.48104575510476366	1.576491884053834E-4
46-47	9.956790846655797E-5	0.0011782202501876026	0.0	0.5225074916553796	1.7424383981647643E-4
48-49	9.956790846655797E-5	0.0011782202501876026	0.0	0.5631477929611464	1.9913581693311593E-4
50-51	9.956790846655797E-5	0.0013607614157096254	0.0	0.6045680428832344	1.9913581693311593E-4
52-53	9.956790846655797E-5	0.0013773560671207184	0.0	1.1652100434813057	1.9913581693311593E-4
54-55	9.956790846655797E-5	0.001402248044237358	0.0	1.917702809043023	1.9913581693311593E-4
56-57	9.956790846655797E-5	0.0014105453699429045	0.0	2.7439422054756375	1.9913581693311593E-4
58-59	9.956790846655797E-5	0.0014105453699429045	0.0	3.591464242342979	1.9913581693311593E-4
60-61	1.0786523417210446E-4	0.0014105453699429045	0.0	3.815027386153224	1.9913581693311593E-4
62-63	1.1616255987765096E-4	0.001418842695648451	0.0	3.823341306510181	2.3232511975530192E-4
64-65	1.3275721128874394E-4	0.0014769239755872763	0.0	3.8314643883759114	2.406224454608484E-4
66-67	1.3275721128874394E-4	0.0014769239755872763	0.0	3.838425844642865	2.489197711663949E-4
68-69	1.3275721128874394E-4	0.0014935186269983693	0.0	3.844748406830491	2.489197711663949E-4
70-71	1.4105453699429045E-4	0.0015101132784094624	0.0	3.8504486695902016	2.821090739885809E-4
72-73	1.4935186269983693E-4	0.0015184106041150089	0.0	3.8551781452423635	3.152983768107669E-4
74-75	1.4935186269983693E-4	0.0015930865354649272	0.0	3.8589534284383866	3.152983768107669E-4
76-77	1.4935186269983693E-4	0.0016511678154037528	0.0	3.8626208464002385	3.152983768107669E-4
78-79	1.4935186269983693E-4	0.0016760597925203923	0.0	3.8651432334147247	3.152983768107669E-4
80-81	1.4935186269983693E-4	0.0016760597925203923	0.0	3.8659314793567514	3.3189302822185984E-4
82-83	1.4935186269983693E-4	0.0016760597925203923	0.0	3.8665122921561395	3.650823310440459E-4
84-85	1.4935186269983693E-4	0.0016760597925203923	0.0	3.8669022664643005	3.650823310440459E-4
86-87	1.4935186269983693E-4	0.0017092490953425784	0.0	3.86712629425835	3.650823310440459E-4
88	1.4935186269983693E-4	0.0018751956094535084	0.0	3.8671345915840556	3.8167698245513885E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	6425	0.0	62.74289	94
GGTATCA	9890	0.0	55.492405	1
TATCTCG	8040	0.0	47.23627	94
GTATCAA	18365	0.0	38.575005	1
GCCGTCT	4090	0.0	32.866253	90-91
TATGCCG	3840	0.0	32.7088	86-87
CCGTCTT	3990	0.0	32.547935	90-91
TCTCGTA	3865	0.0	32.33909	82-83
TGCCGTC	3970	0.0	32.16243	88-89
ATGCCGT	4170	0.0	31.627098	88-89
TCAACGC	22780	0.0	30.701277	4
ATCAACG	22800	0.0	30.564335	3
TATCAAC	23060	0.0	30.425056	2
CTCGTAT	4130	0.0	30.198696	82-83
CAACGCA	23380	0.0	29.840387	5
TCGTATG	4440	0.0	29.35061	84-85
AACGCAG	24070	0.0	28.967243	6
CTATCTC	10105	0.0	27.3905	92-93
CGTATGC	4700	0.0	26.796913	84-85
TGGTATC	4135	0.0	26.627663	2
>>END_MODULE