FastQCFastQC Report
Fri 27 May 2016
SRR2139607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5959185
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141200.055.4126931
GTCAACG7850.040.335951
GTATCAA274150.039.1324771
GCGCAGA14150.035.33241
TGGTATC56900.034.560352
TATCAAC322000.032.942072
GTGGTAT61850.032.939541
TCAACGC329000.032.3284654
ATCAACG328850.032.204393
CAACGCA337900.031.4646285
AACGCAG348450.030.5717566
ACGCAGA391800.027.1041347
GCAACGC9000.026.3864361
CGCAGAG403600.026.3220188
GATCAAC53200.025.2169511
ACCGAAC11450.024.7419288
CCGAACC11700.024.2132579
GCAGAGT445850.023.9491449
TGCGTAT10300.023.04946594
GAGTACT236450.022.1828112-13
Produced by FastQC (version 0.11.5)