##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139607_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5959185
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.099504714151347	25.0	18.0	33.0	18.0	33.0
2	27.55771787585047	28.0	25.0	31.0	18.0	33.0
3	26.006868221073855	28.0	18.0	31.0	18.0	33.0
4	30.59105800541517	31.0	29.0	33.0	27.0	33.0
5	32.37183809531001	33.0	33.0	33.0	32.0	33.0
6	36.70792365063343	38.0	37.0	38.0	34.0	38.0
7	36.90769274657524	38.0	38.0	38.0	35.0	38.0
8	37.124674766767605	38.0	38.0	38.0	36.0	38.0
9	37.30782061640979	38.0	38.0	38.0	36.0	38.0
10-11	37.30384314298012	38.0	38.0	38.0	36.5	38.0
12-13	37.43605853820615	38.0	38.0	38.0	37.0	38.0
14-15	37.42393196385076	38.0	38.0	38.0	37.0	38.0
16-17	37.469363176340394	38.0	38.0	38.0	37.0	38.0
18-19	37.46611961199392	38.0	38.0	38.0	37.0	38.0
20-21	37.47416409723977	38.0	38.0	38.0	37.0	38.0
22-23	37.48536102687048	38.0	38.0	38.0	37.0	38.0
24-25	37.49120652535795	38.0	38.0	38.0	37.0	38.0
26-27	37.45264849476676	38.0	38.0	38.0	37.0	38.0
28-29	37.42686937930162	38.0	38.0	38.0	37.0	38.0
30-31	37.437247332609104	38.0	38.0	38.0	37.0	38.0
32-33	37.42451267445803	38.0	38.0	38.0	37.0	38.0
34-35	37.3953537161102	38.0	38.0	38.0	37.0	38.0
36-37	37.37223101905954	38.0	38.0	38.0	37.0	38.0
38-39	37.36473573650969	38.0	38.0	38.0	37.0	38.0
40-41	37.35472300318958	38.0	38.0	38.0	37.0	38.0
42-43	37.30877822651114	38.0	38.0	38.0	37.0	38.0
44-45	37.259293572873915	38.0	38.0	38.0	37.0	38.0
46-47	37.210986142028474	38.0	38.0	38.0	37.0	38.0
48-49	37.144544934959434	38.0	38.0	38.0	37.0	38.0
50-51	37.10914394224154	38.0	38.0	38.0	37.0	38.0
52-53	37.103201599356865	38.0	38.0	38.0	36.5	38.0
54-55	37.08671644209311	38.0	38.0	38.0	36.0	38.0
56-57	37.03817826005154	38.0	38.0	38.0	36.0	38.0
58-59	37.023162119790925	38.0	38.0	38.0	36.0	38.0
60-61	37.054440203848586	38.0	38.0	38.0	36.0	38.0
62-63	37.068568132285385	38.0	38.0	38.0	36.0	38.0
64-65	37.08466846154819	38.0	38.0	38.0	36.0	38.0
66-67	37.09070132189562	38.0	38.0	38.0	36.0	38.0
68-69	37.091651498622284	38.0	38.0	38.0	36.0	38.0
70-71	37.09477285953912	38.0	38.0	38.0	36.0	38.0
72-73	37.10349315828802	38.0	38.0	38.0	36.0	38.0
74-75	37.08858871552996	38.0	38.0	38.0	36.0	38.0
76-77	37.075672200103256	38.0	38.0	38.0	36.0	38.0
78-79	37.07960629482124	38.0	38.0	38.0	36.0	38.0
80-81	37.08378797698104	38.0	38.0	38.0	36.0	38.0
82-83	37.041033549347674	38.0	38.0	38.0	36.0	38.0
84-85	37.02558217096588	38.0	38.0	38.0	36.0	38.0
86-87	37.03213100943064	38.0	38.0	38.0	36.0	38.0
88-89	37.017977886396324	38.0	38.0	38.0	36.0	38.0
90-91	36.991481077209855	38.0	38.0	38.0	36.0	38.0
92-93	36.98066789788685	38.0	38.0	38.0	36.0	38.0
94-95	36.95803251396063	38.0	38.0	38.0	35.5	38.0
96-97	36.92890591808229	38.0	38.0	38.0	35.0	38.0
98-99	36.89914911801608	38.0	38.0	38.0	35.0	38.0
100	35.5274076506973	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	8.0
19	11.0
20	27.0
21	103.0
22	321.0
23	1005.0
24	2380.0
25	4862.0
26	9042.0
27	15432.0
28	25269.0
29	36789.0
30	49295.0
31	62966.0
32	79827.0
33	108085.0
34	167633.0
35	345517.0
36	1266003.0
37	3784608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.62390746385621	16.34921553870202	10.644375027793231	20.382501969648533
2	14.999729817447212	20.47050697322709	42.04607736887168	22.483685840454015
3	18.991894809566872	27.267364890977895	30.28953917094771	23.451201128507517
4	12.55629754739952	18.09121549339381	39.95759487245319	29.394892086753472
5	12.658111019581039	38.82782253876076	34.92448545379459	13.589580987863606
6	28.695858505293597	38.35878679220301	18.95035170125002	13.995003001253362
7	26.336084548474332	34.12167939072205	23.084012327189036	16.45822373361458
8	24.462942499687458	36.46186517116015	21.1273521463086	17.947840182843795
9	26.74087144466903	16.88833288444645	21.815030075421387	34.55576559546314
10-11	24.067619984947605	27.338654530778957	28.43595223172296	20.157773252550474
12-13	25.234096608848354	25.517012812993723	28.903910517965127	20.344980060192793
14-15	22.755527811269495	26.37675286133926	27.16281672745518	23.704902599936066
16-17	20.89013850048287	29.74913515858293	28.007445649027506	21.353280691906697
18-19	20.811830812434923	28.328655680265	30.718865079704692	20.140648427595384
20-21	22.163832671005977	27.113851567667545	30.74286666762346	19.979449093703018
22-23	22.078582409805602	26.91663436345118	30.59397868063341	20.410804546109805
24-25	21.41506763158158	27.341902186679306	30.78540581174484	20.45762436999427
26-27	21.110385808031037	27.459714174578494	30.74417776254361	20.68572225484686
28-29	20.951848353009904	27.153863142322436	31.056219614708468	20.838068889959185
30-31	21.306805433961678	27.257549622157935	30.878184250004065	20.557460693876326
32-33	20.983296401426585	27.5623641642444	30.664483953275035	20.78985548105398
34-35	21.25655253808072	27.492492781186456	30.754614717240443	20.49633996349238
36-37	21.321260331257363	27.285499952928504	30.517073164088178	20.87616655172596
38-39	21.024723197245436	27.636969564986273	30.618508617452335	20.71979862031596
40-41	21.170749486004176	27.418637463053376	30.69398145750828	20.71663159343417
42-43	21.240136319130738	27.478361887116222	30.199768269678668	21.081733524074377
44-45	21.130498972794427	27.71183018580863	30.096890031411448	21.06078080998549
46-47	21.369179435554056	27.797209225632987	29.659414129159966	21.17419720965299
48-49	21.335447501411796	28.06712138393046	29.316753235875048	21.280677878782694
50-51	21.463664781977005	28.197358525734124	28.800974636147615	21.538002056141256
52-53	21.5859218858521	28.23615795179056	28.68169280147162	21.496227360885722
54-55	21.74947571174373	28.32472371488224	28.551361611895764	21.37443896147827
56-57	21.564279447840494	28.363951106271184	28.443242302436456	21.628527143451866
58-59	21.43736358813734	28.227854164302652	28.67865598997639	21.65612625758361
60-61	21.614765301656288	28.540343696969234	28.49649852953598	21.3483924718385
62-63	21.50211120959492	28.461072861809665	28.687841923635904	21.348974004959516
64-65	21.53084043748589	28.21669271215393	28.678004666753644	21.574462183606542
66-67	21.621354327325854	28.441105266296635	28.34834799789583	21.589192408481686
68-69	21.514964310063743	28.357134791854627	28.598854837656322	21.529046060425312
70-71	21.590196328055537	28.142446072578696	28.752149719446052	21.51520787991971
72-73	21.71500204306068	28.35774360939628	28.350558095392508	21.576696252150537
74-75	21.46225346383302	28.572021221544407	28.36667312916394	21.599052185458635
76-77	21.66133019751926	28.242607082294512	28.530664502584003	21.565398217602223
78-79	21.611707001220935	28.217584412671474	28.422995456940388	21.747713129167202
80-81	21.593159968543258	28.54927464321198	28.137612429846975	21.719952958397784
82-83	21.858459119285477	28.304457477001186	28.284411374217267	21.552672029496065
84-85	21.64985359004445	28.34778434030025	28.24306409210208	21.759297977553214
86-87	21.627208913779885	28.580446072360754	28.368697042660155	21.423647971199205
88-89	21.804539086164365	28.425869359414424	28.29293774606666	21.476653808354552
90-91	21.851488669400947	28.39872016891338	28.31376649005712	21.436024671628555
92-93	21.878365813837775	28.575590743231437	28.15912737770424	21.38691606522655
94-95	21.65640773170667	28.364264472054952	28.361623734714552	21.617704061523828
96-97	21.966752936986193	28.499587753057405	28.289085967520588	21.244573342435817
98-99	22.715693000655545	30.361517180602178	28.629538516709445	18.293251302032832
100	28.949835371431348	40.871263403227026	30.178901225341626	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3241.0
1	2893.5
2	2797.5
3	3185.5
4	4121.5
5	5924.5
6	7149.0
7	7455.0
8	7317.5
9	7180.0
10	7244.0
11	8294.5
12	10622.0
13	15424.5
14	23159.5
15	34539.5
16	46081.5
17	52266.0
18	53572.5
19	47456.0
20	38586.5
21	31689.5
22	27139.5
23	27152.0
24	30270.5
25	36392.0
26	45319.5
27	55929.0
28	67580.0
29	82249.0
30	100322.5
31	117478.5
32	140193.5
33	169804.5
34	190127.5
35	209058.0
36	234635.0
37	249948.0
38	258892.0
39	262701.0
40	262910.5
41	258579.0
42	248954.0
43	249001.5
44	252398.5
45	249743.5
46	240185.0
47	231632.5
48	228269.0
49	223410.5
50	216434.0
51	207123.5
52	196427.5
53	184572.0
54	171425.0
55	159072.0
56	144261.5
57	129185.5
58	116373.5
59	101625.5
60	86605.0
61	72170.5
62	57429.0
63	44852.0
64	35248.0
65	27019.0
66	19306.5
67	14294.0
68	10948.0
69	8144.5
70	6050.0
71	4468.5
72	3284.0
73	2258.0
74	1566.0
75	1140.0
76	749.5
77	438.5
78	297.5
79	216.5
80	156.5
81	111.5
82	83.5
83	55.0
84	35.0
85	25.5
86	16.0
87	10.0
88	5.5
89	2.0
90	1.5
91	1.0
92	0.5
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0042119853637703816
3	0.0027520541819057473
4	0.0
5	3.356163636470424E-5
6	1.3424654545881695E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.0233632917842036E-4
26-27	0.002175861895441183
28-29	0.007767404892372266
30-31	0.002508753487104207
32-33	0.005629787059845818
34-35	0.0030152475818942515
36-37	0.0011235746561733873
38-39	0.0
40-41	8.644752622191201E-5
42-43	0.0024749824158599664
44-45	6.247581262113488E-4
46-47	3.828403477401202E-4
48-49	0.001774284528770294
50-51	5.111574256249972E-4
52-53	0.0
54-55	0.005059748831458927
56-57	0.051454458243889056
58-59	0.07635036155314869
60-61	0.03593282624391625
62-63	0.015291997197823884
64-65	0.00176167286889699
66-67	0.0
68-69	0.0025502519648941315
70-71	0.004300771902541068
72-73	0.014716878171471895
74-75	0.017708382250325776
76-77	0.02357998309351811
78-79	0.0070352168142739785
80-81	0.008567083530242393
82-83	0.03647948478742626
84-85	0.020919942699739975
86-87	0.015321230705829048
88-89	0.007329137794178311
90-91	1.0212132080993101E-4
92-93	0.0
94-95	0.0
96-97	2.3634619186607492E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	798.0
20-21	2697.0
22-23	1708.0
24-25	1769.0
26-27	2969.0
28-29	6481.0
30-31	14680.0
32-33	18934.0
34-35	25882.0
36-37	39640.0
38-39	48376.0
40-41	45787.0
42-43	51836.0
44-45	64773.0
46-47	65831.0
48-49	60554.0
50-51	83025.0
52-53	45566.0
54-55	48507.0
56-57	50257.0
58-59	54606.0
60-61	52421.0
62-63	50388.0
64-65	52358.0
66-67	55338.0
68-69	58323.0
70-71	56981.0
72-73	52167.0
74-75	51648.0
76-77	54464.0
78-79	56934.0
80-81	56489.0
82-83	51880.0
84-85	49282.0
86-87	51058.0
88-89	54084.0
90-91	55067.0
92-93	54116.0
94-95	56942.0
96-97	157885.0
98-99	1277307.0
100-101	2819377.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.41031696149203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.71780372596845	78.38954174011357
2	6.66536638279139	11.519128436029554
3	1.4917626213431112	3.8671104282469337
4	0.528803975179172	1.8277647642291686
5	0.23927070592580463	1.0337728769324364
6	0.11848334686580214	0.614291013439942
7	0.06926759708749368	0.41898045136438755
8	0.044456908895594775	0.30732284710372154
9	0.028017498447740898	0.21789008292036616
>10	0.09320121049201639	1.254278599786322
>50	0.0018952142998410189	0.10469528190384388
>100	0.0014527811626943402	0.29452900203373433
>500	1.8688418187280697E-4	0.10372861818267612
>1k	3.114735900441302E-5	0.04696585771327822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.712327272940846E-5
2	0.0	1.6780818182352115E-5	0.0	0.0	6.712327272940846E-5
3	0.0	1.6780818182352115E-5	0.0	0.0	6.712327272940846E-5
4	0.0	3.356163636470423E-5	0.0	0.0	6.712327272940846E-5
5	0.0	3.356163636470423E-5	0.0	0.0	6.712327272940846E-5
6	1.6780818182352117E-4	3.356163636470423E-5	0.0	0.0	8.390409091176058E-5
7	1.6780818182352117E-4	3.356163636470423E-5	0.0	0.0	8.390409091176058E-5
8	1.6780818182352117E-4	5.034245454705635E-5	0.0	0.0	1.1746572727646481E-4
9	2.181506363705775E-4	1.006849090941127E-4	0.0	0.0	1.1746572727646481E-4
10-11	2.181506363705775E-4	1.006849090941127E-4	0.0	8.390409091176058E-6	1.1746572727646481E-4
12-13	2.181506363705775E-4	1.006849090941127E-4	0.0	2.5171227273528173E-5	1.1746572727646481E-4
14-15	2.181506363705775E-4	1.42636954549993E-4	0.0	3.356163636470423E-5	1.42636954549993E-4
16-17	2.85273909099986E-4	1.5102736364116905E-4	0.0	3.356163636470423E-5	1.5102736364116905E-4
18-19	2.85273909099986E-4	1.9297940909704934E-4	0.0	3.356163636470423E-5	1.5102736364116905E-4
20-21	2.85273909099986E-4	2.2654104546175358E-4	0.0	5.873286363823241E-5	1.594177727323451E-4
22-23	2.85273909099986E-4	2.4332186364410568E-4	0.0	9.229450000293664E-5	1.6780818182352117E-4
24-25	2.93664318191162E-4	2.601026818264578E-4	0.0	1.1746572727646481E-4	1.6780818182352117E-4
26-27	3.3561636364704234E-4	2.6849309091763385E-4	0.0	2.0976022727940146E-4	1.6780818182352117E-4
28-29	3.691780000117466E-4	2.768835000088099E-4	0.0	7.719176363881974E-4	1.6780818182352117E-4
30-31	3.691780000117466E-4	3.020547272823381E-4	0.0	0.002626198045538106	1.6780818182352117E-4
32-33	3.7756840910292265E-4	3.020547272823381E-4	0.0	0.006401882136567332	2.013698181882254E-4
34-35	3.859588181940987E-4	3.607875909205705E-4	0.0	0.01146968922763767	2.181506363705775E-4
36-37	3.859588181940987E-4	4.195204545588029E-4	0.0	0.019046228636969652	2.181506363705775E-4
38-39	4.027396363764508E-4	4.195204545588029E-4	0.0	0.037857525819386374	2.181506363705775E-4
40-41	4.027396363764508E-4	4.6986290910585926E-4	0.0	0.05846437054731478	2.3493145455292963E-4
42-43	4.614725000146832E-4	4.7825331819703533E-4	0.0	0.07031162818405537	2.3493145455292963E-4
44-45	4.6986290910585926E-4	5.285957727440917E-4	0.0	0.08451659077541643	2.4332186364410568E-4
46-47	4.6986290910585926E-4	5.537670000176198E-4	0.0	0.09985425859408628	2.601026818264578E-4
48-49	5.202053636529157E-4	5.621574091087959E-4	0.0	0.11493182373092965	2.85273909099986E-4
50-51	5.70547818199972E-4	6.880135454764368E-4	0.0	0.13281178550422584	2.85273909099986E-4
52-53	5.70547818199972E-4	6.880135454764368E-4	0.0	0.17271657114185918	2.93664318191162E-4
54-55	5.87328636382324E-4	6.964039545676128E-4	0.0	0.21903162932515102	3.020547272823381E-4
56-57	6.041094545646762E-4	7.047943636587889E-4	0.0	0.2826393206453567	3.020547272823381E-4
58-59	6.208902727470283E-4	7.131847727499649E-4	0.0	0.325640167237634	3.1044513637351417E-4
60-61	6.208902727470283E-4	7.299655909323171E-4	0.0	0.3445773205564184	3.1883554546469024E-4
62-63	6.544519091117326E-4	7.467464091146692E-4	0.0	0.3590088241932412	3.3561636364704234E-4
64-65	6.880135454764368E-4	8.054792727529016E-4	0.0	0.3650499187388879	3.3561636364704234E-4
66-67	6.880135454764368E-4	8.054792727529016E-4	0.0	0.3709651571481671	3.3561636364704234E-4
68-69	7.047943636587889E-4	8.474313182087819E-4	0.0	0.3773166968301873	3.3561636364704234E-4
70-71	7.047943636587889E-4	8.7260254548231E-4	0.0	0.38313964073946355	3.3561636364704234E-4
72-73	7.719176363881974E-4	8.893833636646622E-4	0.0	0.3876872424668809	4.195204545588029E-4
74-75	7.970888636617255E-4	9.061641818470143E-4	0.0	0.39097628283062197	4.195204545588029E-4
76-77	8.054792727529016E-4	9.145545909381905E-4	0.0	0.3945673779216453	4.195204545588029E-4
78-79	8.306505000264298E-4	9.229450000293665E-4	0.0	0.39695025410353935	4.195204545588029E-4
80-81	8.390409091176058E-4	9.313354091205425E-4	0.0	0.39787319910356866	4.36301272741155E-4
82-83	8.390409091176058E-4	9.397258182117185E-4	0.0	0.3986199455126833	4.5308209092350716E-4
84-85	8.893833636646622E-4	9.565066363940707E-4	0.0	0.3989975139217863	4.5308209092350716E-4
86-87	8.893833636646622E-4	9.565066363940707E-4	0.0	0.39910658923997155	4.5308209092350716E-4
88	8.893833636646622E-4	0.001006849090941127	0.0	0.39911497964906273	4.5308209092350716E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14120	0.0	55.412693	1
GTCAACG	785	0.0	40.33595	1
GTATCAA	27415	0.0	39.132477	1
GCGCAGA	1415	0.0	35.3324	1
TGGTATC	5690	0.0	34.56035	2
TATCAAC	32200	0.0	32.94207	2
GTGGTAT	6185	0.0	32.93954	1
TCAACGC	32900	0.0	32.328465	4
ATCAACG	32885	0.0	32.20439	3
CAACGCA	33790	0.0	31.464628	5
AACGCAG	34845	0.0	30.571756	6
ACGCAGA	39180	0.0	27.104134	7
GCAACGC	900	0.0	26.386436	1
CGCAGAG	40360	0.0	26.322018	8
GATCAAC	5320	0.0	25.216951	1
ACCGAAC	1145	0.0	24.741928	8
CCGAACC	1170	0.0	24.213257	9
GCAGAGT	44585	0.0	23.949144	9
TGCGTAT	1030	0.0	23.049465	94
GAGTACT	23645	0.0	22.18281	12-13
>>END_MODULE