FastQCFastQC Report
Fri 27 May 2016
SRR2139606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6893076
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101050.054.7401661
GTATCAA192200.039.4382741
TATCAAC227350.032.7741242
TCAACGC228000.032.5841454
ATCAACG228300.032.41063
CAACGCA236150.031.45965
AACGCAG243050.030.7078366
ACGCAGA278450.026.7576127
GTGGTAT48100.025.8928281
GTCAACG8650.025.8174781
CGCAGAG289050.025.7466478
TGGTATC44100.025.6120132
GCGCAGA16650.024.2460041
GATCAAC38100.023.7839531
GCAGAGT330850.022.54579
GTACATG266950.021.5254761
GAGTACT186900.020.8742412-13
TACATGG267100.020.7737642
ACATGGG264400.020.4489943
CAGAGTA324400.019.82353210-11
Produced by FastQC (version 0.11.5)