FastQCFastQC Report
Fri 27 May 2016
SRR2139605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7344400
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105950.052.5097241
GTATCAA195900.037.945531
TCAACGC217900.033.465734
ATCAACG221100.032.8854983
TATCAAC222950.032.7863122
CAACGCA225200.032.3809175
AACGCAG233650.031.2829466
CGAGACC52450.028.68959286-87
AGACCGA56050.027.69263388-89
ACGCAGA266000.027.414227
GAGACCG55100.026.90572788-89
CGCAGAG275400.026.4940158
ACCGAGG59300.026.38962290-91
TGGTATC46150.026.3675292
GACCGAG57700.026.23860490-91
CCCACGA57850.025.4020882-83
GTGGTAT53150.025.3048381
ACGAGAC59000.025.27100286-87
CACGAGA59250.025.21341184-85
GCGCAGA15650.024.8269461
Produced by FastQC (version 0.11.5)