##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139605_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7344400
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.07731223789554	25.0	18.0	33.0	18.0	33.0
2	27.649798485921245	28.0	25.0	31.0	18.0	33.0
3	26.136815805239365	28.0	18.0	31.0	18.0	33.0
4	30.624084608681443	31.0	29.0	33.0	27.0	33.0
5	32.34800623604379	33.0	33.0	33.0	32.0	33.0
6	36.68421722673057	38.0	37.0	38.0	34.0	38.0
7	36.932357578563256	38.0	38.0	38.0	35.0	38.0
8	37.13025284570557	38.0	38.0	38.0	36.0	38.0
9	37.31344439300692	38.0	38.0	38.0	36.0	38.0
10-11	37.32432370241272	38.0	38.0	38.0	36.5	38.0
12-13	37.435529042535805	38.0	38.0	38.0	37.0	38.0
14-15	37.42453093513426	38.0	38.0	38.0	37.0	38.0
16-17	37.456578481564186	38.0	38.0	38.0	37.0	38.0
18-19	37.452357170088774	38.0	38.0	38.0	37.0	38.0
20-21	37.46213466201142	38.0	38.0	38.0	37.0	38.0
22-23	37.46988387530784	38.0	38.0	38.0	37.0	38.0
24-25	37.47674767780093	38.0	38.0	38.0	37.0	38.0
26-27	37.43833811797381	38.0	38.0	38.0	37.0	38.0
28-29	37.41309602999581	38.0	38.0	38.0	37.0	38.0
30-31	37.42315240145436	38.0	38.0	38.0	37.0	38.0
32-33	37.410072889964255	38.0	38.0	38.0	37.0	38.0
34-35	37.384603215876766	38.0	38.0	38.0	37.0	38.0
36-37	37.362386045293036	38.0	38.0	38.0	37.0	38.0
38-39	37.35387787993491	38.0	38.0	38.0	37.0	38.0
40-41	37.34827173801456	38.0	38.0	38.0	37.0	38.0
42-43	37.3091964860296	38.0	38.0	38.0	37.0	38.0
44-45	37.2711257489963	38.0	38.0	38.0	37.0	38.0
46-47	37.24111783264615	38.0	38.0	38.0	37.0	38.0
48-49	37.18860519474373	38.0	38.0	38.0	37.0	38.0
50-51	37.14687481492716	38.0	38.0	38.0	37.0	38.0
52-53	37.134400448677184	38.0	38.0	38.0	36.0	38.0
54-55	37.11044047943855	38.0	38.0	38.0	36.0	38.0
56-57	37.05492633687582	38.0	38.0	38.0	36.0	38.0
58-59	37.02946439806749	38.0	38.0	38.0	36.0	38.0
60-61	37.05692448097524	38.0	38.0	38.0	36.0	38.0
62-63	37.064893864228345	38.0	38.0	38.0	36.0	38.0
64-65	37.07365234913081	38.0	38.0	38.0	36.0	38.0
66-67	37.06996572098944	38.0	38.0	38.0	36.0	38.0
68-69	37.059401177097584	38.0	38.0	38.0	36.0	38.0
70-71	37.051964757419526	38.0	38.0	38.0	36.0	38.0
72-73	37.04662675517309	38.0	38.0	38.0	36.0	38.0
74-75	37.027583730574975	38.0	38.0	38.0	36.0	38.0
76-77	37.00568937767638	38.0	38.0	38.0	36.0	38.0
78-79	37.00868707917492	38.0	38.0	38.0	36.0	38.0
80-81	37.00244667887672	38.0	38.0	38.0	36.0	38.0
82-83	36.95343549566961	38.0	38.0	38.0	36.0	38.0
84-85	36.93944962622781	38.0	38.0	38.0	36.0	38.0
86-87	36.9391621732934	38.0	38.0	38.0	36.0	38.0
88-89	36.92089732922243	38.0	38.0	38.0	35.0	38.0
90-91	36.89911550659111	38.0	38.0	38.0	35.0	38.0
92-93	36.886749389636634	38.0	38.0	38.0	35.0	38.0
94-95	36.85988382456281	38.0	38.0	38.0	35.0	38.0
96-97	36.82775931967042	38.0	38.0	38.0	35.0	38.0
98-99	36.80160582431215	38.0	38.0	38.0	35.0	38.0
100	35.40387238466686	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	5.0
20	19.0
21	104.0
22	392.0
23	1263.0
24	2906.0
25	6010.0
26	11189.0
27	19125.0
28	30604.0
29	44484.0
30	59894.0
31	77141.0
32	99992.0
33	136158.0
34	210123.0
35	417239.0
36	1497446.0
37	4730303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.52588366646697	15.741585425630412	10.91353956756168	21.81899134034094
2	14.960220356986706	20.00379080683048	41.704798270934575	23.331190565248242
3	19.55406873372361	25.237622044855375	30.145749872076376	25.062559349344642
4	13.001810903545557	17.68458961930178	39.345256249659606	29.968343227493055
5	13.662218833396874	37.70414193126736	34.97692119165623	13.65671804367954
6	30.047462050647066	37.69059775387868	18.663963289551187	13.597976905923076
7	26.716722945373345	33.11892053809705	22.77004248134633	17.39431403518327
8	24.036844398453244	37.12099014214912	20.992170905724088	17.849994553673547
9	25.58552094112521	17.64587985403845	21.62651816349872	35.14208104133762
10-11	24.096522520559883	27.155635586297045	28.705380970535373	20.042460922607702
12-13	24.970725995316158	24.83784243777572	28.917304340722183	21.27412722618594
14-15	22.2565492075595	26.30990278307282	26.746133108218505	24.687414901149175
16-17	21.558833941506453	29.3981128478841	27.559718969555036	21.483334241054408
18-19	21.48148929796852	28.35821169870922	29.477261587059527	20.68303741626273
20-21	22.32125265800682	27.498853195238386	29.49259829263952	20.68729585411527
22-23	22.114590993349477	27.2905395712817	29.349987639580654	21.24488179578817
24-25	21.76285592524512	27.502427855928307	29.480200182108767	21.254516036717803
26-27	21.709196987162866	27.606937086922507	29.428992891744006	21.254873034170622
28-29	21.638951592548047	27.59597747368916	29.52899872503506	21.236072208727734
30-31	21.916352150609306	27.489977411031603	29.42304677567314	21.17062366268595
32-33	21.661340888156328	27.726237287774268	29.320062514096794	21.292359309972607
34-35	21.84468357511737	27.719226823664084	29.2839437592026	21.152145842015948
36-37	21.86598151117566	27.551342118646932	29.261848330717726	21.320828039459684
38-39	21.59945395873002	27.806681187176196	29.363217077004826	21.230647777088958
40-41	21.89132002255975	27.649625774586532	29.270819077442017	21.1882351254117
42-43	21.819534994492574	27.738032070915352	29.054547173875356	21.387885760716717
44-45	21.83378926982406	27.85667502314972	28.94055463333107	21.368981073695146
46-47	21.975362531950417	27.856375075703653	28.654844375356266	21.513418016989664
48-49	21.82435250339729	27.97367087225294	28.644040581398983	21.55793604295079
50-51	21.810811405980573	28.11717031171993	28.498754203964005	21.57326407833549
52-53	21.99860648472748	28.048971357635427	28.420509760407747	21.531912397229345
54-55	22.052582430848712	28.00209033188429	28.427227381289693	21.51809985597731
56-57	21.953895075548637	28.11691949360393	28.36085693188949	21.568328498957943
58-59	21.840883472428427	28.012302705050367	28.390622452250152	21.75619137027105
60-61	22.110527480175733	28.00313061015508	28.53976801583989	21.3465738938293
62-63	21.817752579272582	28.03989821090731	28.57740361795124	21.564945591868867
64-65	21.80555759150472	28.00419059415919	28.51708945186955	21.673162362466545
66-67	21.900445941754377	28.128429869299204	28.48668099362991	21.484443195316512
68-69	21.827040734964083	28.30777009813264	28.413876258746562	21.451312908156716
70-71	21.812183893776524	28.125846125560123	28.304920276605962	21.757049704057398
72-73	21.964446393588048	28.18524857583908	28.139361899111826	21.710943131461043
74-75	21.669666838757145	28.26578521746783	28.43344731024342	21.631100633531602
76-77	21.89107758855266	27.827462141566027	28.40226164463883	21.879198625242484
78-79	21.89515342796104	27.92447787180209	28.06071523882505	22.119653461411815
80-81	22.104910295925603	28.15464488679758	28.011995221066936	21.72844959620988
82-83	22.1549293764528	27.841319046236375	28.13634575671568	21.86740582059515
84-85	22.000004888919946	27.770506680076917	28.116439912097217	22.113048518905916
86-87	21.848303483283875	28.102897422285643	28.146592258300167	21.90220683613032
88-89	22.18861113341428	28.081277275303908	28.105691713884124	21.624419877397692
90-91	22.231584451173024	28.109006514533252	28.05624171932993	21.60316731496379
92-93	22.027831542655584	27.88431638785454	28.02160202427222	22.066250045217654
94-95	22.1468130725286	28.141712848859573	28.076384041143704	21.635090037468125
96-97	22.494409485225745	28.002166819205843	28.132861696739216	21.370561998829203
98-99	23.124014191228124	30.038305712278596	28.489455006374463	18.348225090118813
100	29.287011571934197	40.910119879124835	29.80286854894097	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1162.0
1	1158.5
2	1539.5
3	2091.0
4	2613.5
5	3640.5
6	4608.5
7	5618.0
8	7735.5
9	9427.0
10	9506.0
11	9552.5
12	10516.0
13	12837.0
14	17037.5
15	21745.0
16	26172.0
17	30099.5
18	31579.0
19	30076.0
20	27232.0
21	24888.0
22	24914.0
23	28364.0
24	35071.0
25	45078.0
26	59468.5
27	76147.0
28	91342.5
29	107906.5
30	125182.0
31	141662.0
32	161684.5
33	184141.5
34	204418.0
35	222429.0
36	244039.5
37	262240.5
38	273769.5
39	283071.5
40	292943.0
41	300724.0
42	302685.5
43	306727.5
44	311734.5
45	312703.5
46	309436.5
47	306049.5
48	303250.5
49	296817.5
50	287864.5
51	277038.0
52	262744.5
53	246462.0
54	228967.0
55	210355.0
56	190590.5
57	171058.5
58	151966.5
59	131044.5
60	109725.0
61	88698.0
62	69198.0
63	53356.0
64	40488.0
65	30269.0
66	22198.0
67	16724.0
68	12779.0
69	9304.0
70	6822.5
71	4966.5
72	3505.5
73	2403.0
74	1594.0
75	1058.5
76	699.5
77	456.5
78	306.0
79	201.5
80	139.0
81	93.0
82	61.0
83	41.0
84	24.0
85	16.0
86	13.0
87	9.5
88	6.0
89	2.5
90	2.0
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.5
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004316213713850009
3	0.002369152006971298
4	0.0
5	0.0
6	8.169489679211372E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.0426426157754514E-4
26-27	0.001790923748254956
28-29	0.007207137000249252
30-31	0.002407013587557608
32-33	0.005124291746016325
34-35	0.0025760279362008315
36-37	0.0011281851538951179
38-39	2.0756759629043494E-5
40-41	4.872815003647998E-5
42-43	0.0023465118575898454
44-45	6.069705914279097E-4
46-47	3.9876664325574164E-4
48-49	0.0017457613919192655
50-51	4.4187244098811663E-4
52-53	0.0
54-55	0.005019778515318556
56-57	0.049110240822426515
58-59	0.07429381724511397
60-61	0.03480247941391303
62-63	0.014959174550325775
64-65	0.0015574768508551007
66-67	0.0
68-69	0.0021547487667952746
70-71	0.004062023404640308
72-73	0.01403218200430167
74-75	0.01690188929654833
76-77	0.0220872428305594
78-79	0.006616198954493539
80-81	0.008561384424571356
82-83	0.03553345993779684
84-85	0.020621931149886297
86-87	0.014948687099206416
88-89	0.007236889999444111
90-91	9.57301981867482E-5
92-93	0.0
94-95	0.0
96-97	5.427900876036062E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	49.0
20-21	210.0
22-23	601.0
24-25	677.0
26-27	1910.0
28-29	4870.0
30-31	13982.0
32-33	18314.0
34-35	26090.0
36-37	40278.0
38-39	43875.0
40-41	43629.0
42-43	50621.0
44-45	61427.0
46-47	63088.0
48-49	58874.0
50-51	51647.0
52-53	48208.0
54-55	53328.0
56-57	56446.0
58-59	31465.0
60-61	56554.0
62-63	54926.0
64-65	58273.0
66-67	61557.0
68-69	63984.0
70-71	61371.0
72-73	58652.0
74-75	59452.0
76-77	62584.0
78-79	64890.0
80-81	64179.0
82-83	60622.0
84-85	59212.0
86-87	62065.0
88-89	64884.0
90-91	64898.0
92-93	64226.0
94-95	73157.0
96-97	246709.0
98-99	1668195.0
100-101	3644421.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.55102729423614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.60951362369102	82.93277082221088
2	5.471327572585612	9.799260095766419
3	1.1336134322346663	3.0454874223347788
4	0.36910995323285023	1.3221670198611677
5	0.16263959910412212	0.7282271589248431
6	0.08925955933798327	0.4795971140728337
7	0.04750776025086674	0.29780581144393753
8	0.03068820978335585	0.21985285695364354
9	0.021678955870209994	0.17472354919593444
>10	0.06338917359398744	0.8370937443932055
>50	9.239295629907652E-4	0.05390981494917429
>100	3.0654606460953706E-4	0.06759466883788093
>500	2.0842344747634362E-5	0.016032834563159327
>1k	2.084234295524826E-5	0.025477086492056298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3615816132018953E-5	0.0	0.0	0.0
3	0.0	1.3615816132018953E-5	0.0	1.3615816132018953E-5	0.0
4	0.0	1.3615816132018953E-5	0.0	2.7231632264037906E-5	0.0
5	0.0	1.3615816132018953E-5	0.0	2.7231632264037906E-5	0.0
6	5.446326452807581E-5	1.3615816132018953E-5	0.0	2.7231632264037906E-5	0.0
7	5.446326452807581E-5	1.3615816132018953E-5	0.0	2.7231632264037906E-5	0.0
8	5.446326452807581E-5	4.084744839605686E-5	0.0	4.084744839605686E-5	1.3615816132018953E-5
9	6.807908066009477E-5	9.531071292413267E-5	0.0	5.446326452807581E-5	4.084744839605686E-5
10-11	8.169489679211372E-5	9.531071292413267E-5	0.0	6.12711725940853E-5	4.084744839605686E-5
12-13	8.169489679211372E-5	9.531071292413267E-5	0.0	6.807908066009477E-5	4.084744839605686E-5
14-15	8.169489679211372E-5	1.770056097162464E-4	0.0	6.807908066009477E-5	4.7655356462066336E-5
16-17	8.169489679211372E-5	1.9062142584826535E-4	0.0	6.807908066009477E-5	5.446326452807581E-5
18-19	8.169489679211372E-5	2.042372419802843E-4	0.0	1.0892652905615162E-4	6.807908066009477E-5
20-21	8.169489679211372E-5	2.1785305811230325E-4	0.0	1.0892652905615162E-4	6.807908066009477E-5
22-23	8.169489679211372E-5	2.1785305811230325E-4	0.0	1.225423451881706E-4	6.807908066009477E-5
24-25	8.169489679211372E-5	2.314688742443222E-4	0.0	2.2466096617831274E-4	7.488698872610425E-5
26-27	8.169489679211372E-5	2.314688742443222E-4	0.0	3.1316377103643596E-4	8.85028048581232E-5
28-29	8.169489679211372E-5	2.382767823103317E-4	0.0	5.310168291487392E-4	9.531071292413267E-5
30-31	9.531071292413267E-5	2.927400468384075E-4	0.0	0.0015930504874462174	9.531071292413267E-5
32-33	9.531071292413267E-5	2.99547954904417E-4	0.0	0.003914547137955449	9.531071292413267E-5
34-35	9.531071292413267E-5	3.472033113664833E-4	0.0	0.006787484341811448	9.531071292413267E-5
36-37	9.531071292413267E-5	3.9485866782854967E-4	0.0	0.012131692173628889	9.531071292413267E-5
38-39	9.531071292413267E-5	4.2209030009258757E-4	0.0	0.02614236697347639	9.531071292413267E-5
40-41	1.0211862099014215E-4	4.493219323566255E-4	0.0	0.04106530145416916	9.531071292413267E-5
42-43	1.0892652905615162E-4	4.697456565546539E-4	0.0	0.04930287021404063	9.531071292413267E-5
44-45	1.157344371221611E-4	5.310168291487392E-4	0.0	0.05956919557758292	9.531071292413267E-5
46-47	1.225423451881706E-4	5.71864277544796E-4	0.0	0.07031207450574586	1.0892652905615162E-4
48-49	1.225423451881706E-4	5.71864277544796E-4	0.0	0.08123195904362507	1.225423451881706E-4
50-51	1.225423451881706E-4	6.944066227329666E-4	0.0	0.09257393388159686	1.3615816132018954E-4
52-53	1.225423451881706E-4	7.080224388649856E-4	0.0	0.12706960405206688	1.5658188551821798E-4
54-55	1.225423451881706E-4	7.148303469309951E-4	0.0	0.1706878710309896	1.6338979358422744E-4
56-57	1.225423451881706E-4	7.352540711290234E-4	0.0	0.2240754860846359	1.6338979358422744E-4
58-59	1.225423451881706E-4	7.624857033930614E-4	0.0	0.4397500136158161	1.701977016502369E-4
60-61	1.225423451881706E-4	8.169489679211372E-4	0.0	0.6334349980937857	1.770056097162464E-4
62-63	1.225423451881706E-4	8.305647840531562E-4	0.0	0.6400046293774849	2.1104515004629379E-4
64-65	1.225423451881706E-4	9.394913131093078E-4	0.0	0.6462066336256196	2.1785305811230325E-4
66-67	1.225423451881706E-4	9.394913131093078E-4	0.0	0.6522452480801699	2.1785305811230325E-4
68-69	1.225423451881706E-4	9.667229453733456E-4	0.0	0.6584812918686346	2.1785305811230325E-4
70-71	1.225423451881706E-4	9.803387615053647E-4	0.0	0.6636553019988018	2.1785305811230325E-4
72-73	1.3615816132018954E-4	0.001000762485703393	0.0	0.6678149338271335	2.450846903763412E-4
74-75	1.3615816132018954E-4	0.0010484178421654594	0.0	0.670953379445564	2.723163226403791E-4
76-77	1.42966069386199E-4	0.0010824573824955068	0.0	0.6741803278688525	2.791242307063885E-4
78-79	1.6338979358422744E-4	0.0010892652905615163	0.0	0.6761273895757312	2.85932138772398E-4
80-81	1.6338979358422744E-4	0.0010960731986275258	0.0	0.676706061761342	3.2677958716845483E-4
82-83	1.6338979358422744E-4	0.001116496922825554	0.0	0.6773323893034149	3.676270355645117E-4
84-85	1.6338979358422744E-4	0.001130112738957573	0.0	0.6776319372583193	3.676270355645117E-4
86-87	1.6338979358422744E-4	0.001143728555089592	0.0	0.6777272479712434	3.676270355645117E-4
88	1.6338979358422744E-4	0.0012662709002777626	0.0	0.6777544796035074	3.676270355645117E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10595	0.0	52.509724	1
GTATCAA	19590	0.0	37.94553	1
TCAACGC	21790	0.0	33.46573	4
ATCAACG	22110	0.0	32.885498	3
TATCAAC	22295	0.0	32.786312	2
CAACGCA	22520	0.0	32.380917	5
AACGCAG	23365	0.0	31.282946	6
CGAGACC	5245	0.0	28.689592	86-87
AGACCGA	5605	0.0	27.692633	88-89
ACGCAGA	26600	0.0	27.41422	7
GAGACCG	5510	0.0	26.905727	88-89
CGCAGAG	27540	0.0	26.494015	8
ACCGAGG	5930	0.0	26.389622	90-91
TGGTATC	4615	0.0	26.367529	2
GACCGAG	5770	0.0	26.238604	90-91
CCCACGA	5785	0.0	25.40208	82-83
GTGGTAT	5315	0.0	25.304838	1
ACGAGAC	5900	0.0	25.271002	86-87
CACGAGA	5925	0.0	25.213411	84-85
GCGCAGA	1565	0.0	24.826946	1
>>END_MODULE