FastQCFastQC Report
Fri 27 May 2016
SRR2139604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5575256
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87800.053.31891
GTATCAA159400.036.006511
TCAACGC192600.029.2744944
ATCAACG195100.028.9647083
TATCAAC198600.028.6246532
CAACGCA197250.028.6056565
AACGCAG204400.027.6050196
TGGTATC37650.027.0983032
GTGGTAT43300.025.69561
ACGCAGA233800.024.151477
CGCAGAG240750.023.4542628
GCAGAGT275500.020.5263529
GAGTACT155250.020.2001612-13
CAGAGTA267450.019.27620510-11
GTACTTT165900.018.9415314-15
GTACATG202300.018.1183721
TACATGG201400.017.9074822
AGAGTAC253300.017.4359810-11
ACATGGG203150.017.0497683
AGTACTT169300.016.26719512-13
Produced by FastQC (version 0.11.5)