##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139604_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5575256
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.41002224830573	32.0	25.0	33.0	18.0	33.0
2	29.6221784255288	31.0	28.0	33.0	25.0	33.0
3	31.999581005787	33.0	31.0	33.0	29.0	34.0
4	31.84319572051938	33.0	31.0	33.0	29.0	34.0
5	32.83956539394783	33.0	33.0	34.0	32.0	34.0
6	36.801409657242644	38.0	37.0	38.0	34.0	38.0
7	36.95503793906504	38.0	38.0	38.0	35.0	38.0
8	37.10246435320638	38.0	38.0	38.0	36.0	38.0
9	37.17819863339011	38.0	38.0	38.0	36.0	38.0
10-11	37.299902013468085	38.0	38.0	38.0	36.5	38.0
12-13	37.36178885776725	38.0	38.0	38.0	37.0	38.0
14-15	37.37219447860331	38.0	38.0	38.0	37.0	38.0
16-17	37.43235144718018	38.0	38.0	38.0	37.0	38.0
18-19	37.422012191009706	38.0	38.0	38.0	37.0	38.0
20-21	37.43529435300042	38.0	38.0	38.0	37.0	38.0
22-23	37.44400988963325	38.0	38.0	38.0	37.0	38.0
24-25	37.45977957883383	38.0	38.0	38.0	37.0	38.0
26-27	37.419118434247764	38.0	38.0	38.0	37.0	38.0
28-29	37.386122936247915	38.0	38.0	38.0	37.0	38.0
30-31	37.40462475956774	38.0	38.0	38.0	37.0	38.0
32-33	37.39644295518528	38.0	38.0	38.0	37.0	38.0
34-35	37.365507335065814	38.0	38.0	38.0	37.0	38.0
36-37	37.34340300440675	38.0	38.0	38.0	37.0	38.0
38-39	37.348266619233065	38.0	38.0	38.0	37.0	38.0
40-41	37.349488056815865	38.0	38.0	38.0	37.0	38.0
42-43	37.31049940654829	38.0	38.0	38.0	37.0	38.0
44-45	37.27357828965421	38.0	38.0	38.0	37.0	38.0
46-47	37.24773910164629	38.0	38.0	38.0	37.0	38.0
48-49	37.190022771839125	38.0	38.0	38.0	37.0	38.0
50-51	37.14843154014571	38.0	38.0	38.0	37.0	38.0
52-53	37.13872550464337	38.0	38.0	38.0	37.0	38.0
54-55	37.12451807349933	38.0	38.0	38.0	36.5	38.0
56-57	37.076509671739345	38.0	38.0	38.0	36.0	38.0
58-59	37.0607664828929	38.0	38.0	38.0	36.0	38.0
60-61	37.08816374874097	38.0	38.0	38.0	36.0	38.0
62-63	37.102736335063256	38.0	38.0	38.0	36.0	38.0
64-65	37.11302703742108	38.0	38.0	38.0	36.0	38.0
66-67	37.119331867479644	38.0	38.0	38.0	36.0	38.0
68-69	37.116607183360145	38.0	38.0	38.0	36.0	38.0
70-71	37.1133840651388	38.0	38.0	38.0	36.0	38.0
72-73	37.112869687794614	38.0	38.0	38.0	36.0	38.0
74-75	37.09814821677851	38.0	38.0	38.0	36.0	38.0
76-77	37.081373235684666	38.0	38.0	38.0	36.0	38.0
78-79	37.08695082239256	38.0	38.0	38.0	36.0	38.0
80-81	37.08906805498488	38.0	38.0	38.0	36.0	38.0
82-83	37.042369340037894	38.0	38.0	38.0	36.0	38.0
84-85	37.03150282917116	38.0	38.0	38.0	36.0	38.0
86-87	37.03379801096273	38.0	38.0	38.0	36.0	38.0
88-89	37.02526726142729	38.0	38.0	38.0	36.0	38.0
90-91	37.00478452325278	38.0	38.0	38.0	36.0	38.0
92-93	36.9994869769091	38.0	38.0	38.0	36.0	38.0
94-95	36.98075812147589	38.0	38.0	38.0	36.0	38.0
96-97	36.95386489373243	38.0	38.0	38.0	35.0	38.0
98-99	36.92597263674848	38.0	38.0	38.0	35.0	38.0
100	35.61531847769278	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	15.0
21	53.0
22	280.0
23	895.0
24	2121.0
25	4438.0
26	8158.0
27	13787.0
28	22126.0
29	33066.0
30	44492.0
31	57290.0
32	73416.0
33	97902.0
34	147196.0
35	272553.0
36	823424.0
37	3974036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34583237074674	20.978103965091467	14.158094265088456	23.517969399073337
2	15.239826280118645	20.621056468288877	41.75164198723146	22.38747526436102
3	18.357856801029154	26.28888114010448	30.248545847333162	25.10471621153321
4	13.01265807345887	17.80296725388036	38.60999387292709	30.57438079973368
5	13.601788762194067	37.32172106301204	35.3888218864367	13.687668288357191
6	30.13526956158643	37.73467608019604	18.452665159765377	13.677389198452156
7	26.643260865510033	32.89326983370808	22.889137288045607	17.574332012736278
8	24.344227422023312	36.84704343621172	20.92551445171307	17.8832146900519
9	25.75205515226565	17.115016781292194	21.42116882166487	35.711759244777284
10-11	24.31199571822352	26.862407753114837	28.61599539106366	20.20960113759799
12-13	25.200340576289232	24.55936552509876	28.85628928967567	21.384004608936344
14-15	22.382676239440844	25.82518183918371	26.775470041196314	25.016671880179135
16-17	21.625491277889303	28.927120117892347	27.496244118655717	21.951144485562637
18-19	21.633203928214236	27.899481207679074	29.498636475168137	20.968678388938553
20-21	22.61362212648278	26.9983694759732	29.401000125286952	20.987008272257064
22-23	22.290209045430384	26.824933342290088	29.31821734651148	21.56664026576805
24-25	21.995819118857682	26.96752650453688	29.463336384770226	21.573317991835207
26-27	21.86342266252219	27.172783966910696	29.400243849335165	21.563549521231945
28-29	21.787988227162664	27.150475604785857	29.48980332741604	21.57173284063544
30-31	22.095669832682784	27.035172529114266	29.322424269761214	21.546733368441735
32-33	21.820398018107	27.273207168210295	29.249992478088714	21.65640233559399
34-35	22.010898571305777	27.23335887257605	29.285678197045755	21.470064359072413
36-37	22.00917882820532	27.06222093855471	29.245276164110834	21.683324069129128
38-39	21.79802899343955	27.291551687015588	29.33637083884946	21.574048480695403
40-41	22.11196044843905	27.143484550401926	29.18214299375048	21.562412007408543
42-43	22.01941638626029	27.241361404347092	28.921161490384932	21.818060719007683
44-45	22.015526857536962	27.34549953557064	28.870742375356244	21.768231231536156
46-47	22.128705378918536	27.333325056854203	28.563102451471007	21.974867112756254
48-49	22.088145360427223	27.410275580196814	28.523394156857762	21.9781849025182
50-51	22.042208702885162	27.576742656517496	28.360195761720075	22.02085287887727
52-53	22.24348389308902	27.5357469769446	28.20765090346489	22.013118226501486
54-55	22.241489625866258	27.498498845186038	28.233146935227964	22.026864593719743
56-57	22.145653270870717	27.59650162181671	28.228484471360886	22.029360635951683
58-59	22.113167379506972	27.55395078391023	28.264827332601627	22.068054503981173
60-61	22.176327464248594	27.594647932932386	28.274224032749988	21.954800570069025
62-63	22.095389413815997	27.617357339683807	28.311901721944338	21.975351524555858
64-65	22.066120840210896	27.55170976605549	28.3442919952613	22.037877398472315
66-67	22.13440892191757	27.764303356049247	28.050886984939883	22.0504007370933
68-69	22.107484789838473	27.658821893687	28.221688064730717	22.012005251743812
70-71	22.117536942249874	27.34663655081708	28.367436265091307	22.16839024184174
72-73	22.25307344108384	27.48208361450843	28.120903368266454	22.143939576141282
74-75	22.00952802102979	27.67677888492702	28.101056710658217	22.212636383384964
76-77	22.264188245624553	27.47504152399223	28.000450063652487	22.26032016673073
78-79	22.259933188022707	27.513596678750122	27.9430528873911	22.283417245836066
80-81	22.24501065009689	27.560607882222087	27.858005324710096	22.336376142970924
82-83	22.370594561225733	27.493321361267697	27.922708490361103	22.21337558714546
84-85	22.455684550724275	27.51560198335265	27.812996397891084	22.21571706803199
86-87	22.256348838256955	27.581520882851418	27.93411806006663	22.22801221882499
88-89	22.34575185106516	27.56858277438216	27.868302370966784	22.21736300358589
90-91	22.417416084003428	27.601845000323312	27.795005648045546	22.185733267627718
92-93	22.49413215703795	27.59625126033377	27.79541182842906	22.114204754199214
94-95	22.35134047482033	27.65876933363005	27.814068245741137	22.175821945808487
96-97	22.507491544940358	27.700387063674608	27.8771228329177	21.91499855846733
98-99	23.485833109703556	29.66425865716948	28.05756297931503	18.79234525381193
100	29.990706513992492	40.649289684990734	29.36000380101677	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1606.0
1	1409.0
2	1468.0
3	1865.5
4	2430.5
5	3476.0
6	4470.5
7	5789.5
8	7282.5
9	8092.5
10	8082.5
11	7880.5
12	8668.5
13	11010.0
14	15753.5
15	21106.0
16	24829.5
17	26819.0
18	26518.0
19	24183.0
20	21219.5
21	18734.5
22	18160.5
23	20184.5
24	24699.0
25	32061.5
26	42602.0
27	54218.5
28	65467.5
29	78278.5
30	90392.0
31	101984.5
32	116916.0
33	134059.0
34	150051.0
35	165532.5
36	182640.5
37	194931.5
38	203489.5
39	210979.5
40	218512.0
41	225208.0
42	227994.5
43	232211.0
44	237055.5
45	239675.0
46	239886.0
47	239068.5
48	238420.5
49	237261.0
50	232913.5
51	225717.5
52	216030.0
53	203196.0
54	189433.0
55	175326.5
56	159468.5
57	143141.0
58	127622.5
59	110355.5
60	91938.0
61	73739.0
62	57077.0
63	43538.5
64	33099.0
65	24769.0
66	17899.0
67	13385.5
68	10094.0
69	7355.0
70	5436.0
71	3834.5
72	2585.5
73	1746.5
74	1188.0
75	798.5
76	522.5
77	324.5
78	215.0
79	141.0
80	89.5
81	52.5
82	34.0
83	32.0
84	26.0
85	17.0
86	8.0
87	3.5
88	3.0
89	3.5
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005452664415768532
3	0.003372042467646329
4	0.0
5	7.174558441800699E-5
6	3.228551298810315E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.6145142680007576E-4
26-27	0.001955636651680928
28-29	0.00782568747811926
30-31	0.00280332240426946
32-33	0.005413681372693409
34-35	0.0030638921842550647
36-37	0.0011898003051883195
38-39	3.6586153348300724E-5
40-41	1.1981955543626852E-4
42-43	0.0026381020899063335
44-45	7.781838501536678E-4
46-47	4.6436518436245505E-4
48-49	0.0020399877830590794
50-51	5.51302199976265E-4
52-53	0.0
54-55	0.004930611242266026
56-57	0.051804507111300836
58-59	0.07780053339195284
60-61	0.0363506683853811
62-63	0.01584093694898032
64-65	0.0016625661863984532
66-67	0.0
68-69	0.00236293350599626
70-71	0.004373597848531212
72-73	0.015139909913222652
74-75	0.01826486207795064
76-77	0.02420544817593714
78-79	0.0070324624484894415
80-81	0.008322691265143801
82-83	0.036788463028450465
84-85	0.021126707747017193
86-87	0.015321367730212303
88-89	0.007402413871674964
90-91	1.0757032110936078E-4
92-93	0.0
94-95	0.0
96-97	3.73938295693951E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24.0
20-21	188.0
22-23	503.0
24-25	670.0
26-27	1614.0
28-29	4309.0
30-31	13304.0
32-33	17172.0
34-35	23699.0
36-37	36848.0
38-39	41861.0
40-41	41452.0
42-43	47100.0
44-45	55892.0
46-47	56571.0
48-49	52282.0
50-51	46926.0
52-53	42933.0
54-55	46368.0
56-57	47204.0
58-59	49348.0
60-61	48111.0
62-63	46842.0
64-65	49389.0
66-67	51647.0
68-69	52680.0
70-71	51486.0
72-73	48746.0
74-75	49134.0
76-77	51415.0
78-79	52537.0
80-81	52827.0
82-83	48740.0
84-85	48390.0
86-87	49420.0
88-89	51149.0
90-91	51998.0
92-93	50836.0
94-95	57728.0
96-97	165884.0
98-99	1239154.0
100-101	2630875.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.90192706946345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.78174797040121	80.67792516402083
2	5.9250877217655145	10.416532575976113
3	1.3123091850228081	3.4606351882338577
4	0.4566762935462993	1.6057090499863875
5	0.20213006265779435	0.8883811013145759
6	0.10543026108257136	0.5560513872356807
7	0.05773451611610642	0.35524826575201246
8	0.038352063756778056	0.2696976249049374
9	0.028273213806012696	0.22367429801959277
>10	0.08939587337335499	1.182564783420082
>50	0.0016617253049170347	0.09470604288201864
>100	0.0011101564248226692	0.202087338922414
>500	6.06378282569676E-5	0.03819075364091282
>1k	3.0318913588529785E-5	0.02859642569053465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7936396104501747E-5
2	0.0	0.0	0.0	1.7936396104501747E-5	1.7936396104501747E-5
3	0.0	0.0	0.0	1.7936396104501747E-5	1.7936396104501747E-5
4	0.0	7.174558441800699E-5	0.0	1.7936396104501747E-5	3.5872792209003494E-5
5	0.0	7.174558441800699E-5	0.0	1.7936396104501747E-5	3.5872792209003494E-5
6	5.3809188313505244E-5	7.174558441800699E-5	0.0	1.7936396104501747E-5	3.5872792209003494E-5
7	5.3809188313505244E-5	7.174558441800699E-5	0.0	1.7936396104501747E-5	3.5872792209003494E-5
8	5.3809188313505244E-5	7.174558441800699E-5	0.0	1.7936396104501747E-5	3.5872792209003494E-5
9	5.3809188313505244E-5	1.2555477273151224E-4	0.0	1.7936396104501747E-5	3.5872792209003494E-5
10-11	5.3809188313505244E-5	1.2555477273151224E-4	0.0	7.174558441800699E-5	3.5872792209003494E-5
12-13	5.3809188313505244E-5	1.2555477273151224E-4	0.0	1.1658657467926136E-4	3.5872792209003494E-5
14-15	5.3809188313505244E-5	1.9730035714951924E-4	0.0	1.345229707837631E-4	7.174558441800699E-5
16-17	1.6142756494051574E-4	1.9730035714951924E-4	0.0	1.6142756494051574E-4	8.968198052250874E-5
18-19	1.6142756494051574E-4	2.3317314935852274E-4	0.0	2.86982337672028E-4	8.968198052250874E-5
20-21	1.6142756494051574E-4	2.8698233767202795E-4	0.0	3.1388693182878057E-4	8.968198052250874E-5
22-23	1.6142756494051574E-4	3.228551298810315E-4	0.0	3.228551298810315E-4	1.1658657467926136E-4
24-25	1.6142756494051574E-4	3.6769612014228586E-4	0.0	3.4079152598553324E-4	1.4349116883601398E-4
26-27	1.6142756494051574E-4	3.946007142990385E-4	0.0	5.02219090926049E-4	1.6142756494051574E-4
28-29	1.6142756494051574E-4	3.946007142990385E-4	0.0	9.416607954863417E-4	1.6142756494051574E-4
30-31	1.6142756494051574E-4	4.3047350650804195E-4	0.0	0.0021792721266969623	1.6142756494051574E-4
32-33	1.6142756494051574E-4	4.3047350650804195E-4	0.0	0.005165682078096504	1.7936396104501748E-4
34-35	1.9730035714951924E-4	4.5737810066479457E-4	0.0	0.008896452467832867	1.7936396104501748E-4
36-37	1.9730035714951924E-4	4.842826948215472E-4	0.0	0.01565847379923003	1.7936396104501748E-4
38-39	1.9730035714951924E-4	5.02219090926049E-4	0.0	0.03410605719271008	1.9730035714951924E-4
40-41	1.9730035714951924E-4	5.02219090926049E-4	0.0	0.05442799397911056	1.9730035714951924E-4
42-43	2.1523675325402098E-4	5.02219090926049E-4	0.0	0.06571895532689441	2.062685552017701E-4
44-45	2.2420495130627186E-4	5.470600811873033E-4	0.0	0.07958378951567426	2.1523675325402098E-4
46-47	2.3317314935852274E-4	5.919010714485577E-4	0.0	0.09356521027913337	2.1523675325402098E-4
48-49	2.3317314935852274E-4	6.008692695008086E-4	0.0	0.10664981123736739	2.4214134741077362E-4
50-51	2.511095454630245E-4	7.443604383368226E-4	0.0	0.12102583271512554	2.511095454630245E-4
52-53	2.6904594156752624E-4	7.533286363890734E-4	0.0	0.16494309857699807	2.6007774351527533E-4
54-55	2.780141396197771E-4	7.533286363890734E-4	0.0	0.22087774982888678	2.6904594156752624E-4
56-57	2.8698233767202795E-4	7.533286363890734E-4	0.0	0.2971433060652282	2.6904594156752624E-4
58-59	3.049187337765297E-4	7.533286363890734E-4	0.0	0.3431322256771707	2.780141396197771E-4
60-61	3.4079152598553324E-4	7.622968344413243E-4	0.0	0.35858443092119896	2.8698233767202795E-4
62-63	3.5872792209003495E-4	7.712650324935751E-4	0.0	0.37153450890864925	2.8698233767202795E-4
64-65	3.5872792209003495E-4	8.609470130160839E-4	0.0	0.3788346221231814	2.8698233767202795E-4
66-67	3.766643181945367E-4	8.878516071728366E-4	0.0	0.3857939438117281	2.8698233767202795E-4
68-69	3.946007142990385E-4	9.326925974340909E-4	0.0	0.39188335028920646	2.8698233767202795E-4
70-71	4.0356891235128933E-4	9.775335876953453E-4	0.0	0.3974615694777065	2.8698233767202795E-4
72-73	4.484099026125437E-4	9.865017857475962E-4	0.0	0.40193670030577966	3.5872792209003495E-4
74-75	4.484099026125437E-4	0.001004438181852098	0.0	0.40559572511109804	3.5872792209003495E-4
76-77	4.484099026125437E-4	0.001058247370165603	0.0	0.40920990892615516	3.5872792209003495E-4
78-79	4.484099026125437E-4	0.0010941201623746067	0.0	0.41178378176715114	3.5872792209003495E-4
80-81	4.484099026125437E-4	0.0010941201623746067	0.0	0.4125729831957492	3.5872792209003495E-4
82-83	4.484099026125437E-4	0.0010941201623746067	0.0	0.413245598049668	3.946007142990385E-4
84-85	4.484099026125437E-4	0.0010941201623746067	0.0	0.41357742137760134	3.946007142990385E-4
86-87	4.5737810066479457E-4	0.0011479293506881118	0.0	0.41374781714059405	3.946007142990385E-4
88	4.663462987170455E-4	0.0012017385390016171	0.0	0.41375678533864635	3.946007142990385E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8780	0.0	53.3189	1
GTATCAA	15940	0.0	36.00651	1
TCAACGC	19260	0.0	29.274494	4
ATCAACG	19510	0.0	28.964708	3
TATCAAC	19860	0.0	28.624653	2
CAACGCA	19725	0.0	28.605656	5
AACGCAG	20440	0.0	27.605019	6
TGGTATC	3765	0.0	27.098303	2
GTGGTAT	4330	0.0	25.6956	1
ACGCAGA	23380	0.0	24.15147	7
CGCAGAG	24075	0.0	23.454262	8
GCAGAGT	27550	0.0	20.526352	9
GAGTACT	15525	0.0	20.20016	12-13
CAGAGTA	26745	0.0	19.276205	10-11
GTACTTT	16590	0.0	18.94153	14-15
GTACATG	20230	0.0	18.118372	1
TACATGG	20140	0.0	17.907482	2
AGAGTAC	25330	0.0	17.43598	10-11
ACATGGG	20315	0.0	17.049768	3
AGTACTT	16930	0.0	16.267195	12-13
>>END_MODULE