FastQCFastQC Report
Fri 27 May 2016
SRR2139603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4003646
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83300.055.13881
GTATCAA157750.036.459981
TGGTATC33700.030.854132
TCAACGC192750.029.3272154
ATCAACG195750.028.8788363
TATCAAC197500.028.748742
CAACGCA198450.028.484865
AACGCAG205200.027.5877536
GTGGTAT40000.026.915671
ACCGAAC12350.025.5208598
GGACCGA12850.024.5278266
AGGACCG13700.024.4999265
ACGCAGA232150.024.3498617
CGCAGAG234600.024.1130168
GACCGAA13250.022.8605867
CCGAACC13750.022.6246789
TTAGCCG6150.022.6032194
TGCGTAT7150.022.03417694
GCAGAGT261550.021.7692729
GAGTACT131200.021.24637212-13
Produced by FastQC (version 0.11.5)