##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4003646
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.662326039814708	32.0	25.0	33.0	18.0	34.0
2	29.991046161423863	31.0	29.0	33.0	25.0	33.0
3	32.13025127596196	33.0	31.0	33.0	30.0	34.0
4	32.00955129399552	33.0	31.0	33.0	29.0	34.0
5	32.89866861355874	33.0	33.0	34.0	32.0	34.0
6	36.87479062834227	38.0	37.0	38.0	35.0	38.0
7	37.05782354383979	38.0	38.0	38.0	35.0	38.0
8	37.174790928069065	38.0	38.0	38.0	36.0	38.0
9	37.255774861214	38.0	38.0	38.0	36.0	38.0
10-11	37.3547424023003	38.0	38.0	38.0	37.0	38.0
12-13	37.413500469322216	38.0	38.0	38.0	37.0	38.0
14-15	37.419791859719865	38.0	38.0	38.0	37.0	38.0
16-17	37.46915848703907	38.0	38.0	38.0	37.0	38.0
18-19	37.46317019536692	38.0	38.0	38.0	37.0	38.0
20-21	37.47637301341551	38.0	38.0	38.0	37.0	38.0
22-23	37.48445439168385	38.0	38.0	38.0	37.0	38.0
24-25	37.49542154500115	38.0	38.0	38.0	37.0	38.0
26-27	37.46227731772407	38.0	38.0	38.0	37.0	38.0
28-29	37.43602899818899	38.0	38.0	38.0	37.0	38.0
30-31	37.45064989582418	38.0	38.0	38.0	37.0	38.0
32-33	37.440196209370484	38.0	38.0	38.0	37.0	38.0
34-35	37.410439557825	38.0	38.0	38.0	37.0	38.0
36-37	37.39021972339738	38.0	38.0	38.0	37.0	38.0
38-39	37.379435342342816	38.0	38.0	38.0	37.0	38.0
40-41	37.392953278981004	38.0	38.0	38.0	37.0	38.0
42-43	37.3535594741697	38.0	38.0	38.0	37.0	38.0
44-45	37.31123970428408	38.0	38.0	38.0	37.0	38.0
46-47	37.270956907394435	38.0	38.0	38.0	37.0	38.0
48-49	37.21105459470199	38.0	38.0	38.0	37.0	38.0
50-51	37.16646220405254	38.0	38.0	38.0	37.0	38.0
52-53	37.160374206403986	38.0	38.0	38.0	37.0	38.0
54-55	37.14443907749731	38.0	38.0	38.0	37.0	38.0
56-57	37.09893751799288	38.0	38.0	38.0	36.0	38.0
58-59	37.07548708199869	38.0	38.0	38.0	36.0	38.0
60-61	37.101922124705936	38.0	38.0	38.0	36.0	38.0
62-63	37.11364413582467	38.0	38.0	38.0	36.0	38.0
64-65	37.127781372272594	38.0	38.0	38.0	36.0	38.0
66-67	37.13084722698645	38.0	38.0	38.0	36.0	38.0
68-69	37.12362978978318	38.0	38.0	38.0	36.0	38.0
70-71	37.12743831662307	38.0	38.0	38.0	36.0	38.0
72-73	37.12598975758829	38.0	38.0	38.0	36.0	38.0
74-75	37.108936469728214	38.0	38.0	38.0	36.0	38.0
76-77	37.09734865414818	38.0	38.0	38.0	36.0	38.0
78-79	37.10625395136863	38.0	38.0	38.0	36.0	38.0
80-81	37.11106465589202	38.0	38.0	38.0	36.0	38.0
82-83	37.06746683422456	38.0	38.0	38.0	36.0	38.0
84-85	37.05779246509496	38.0	38.0	38.0	36.0	38.0
86-87	37.06672416452531	38.0	38.0	38.0	36.0	38.0
88-89	37.05776267408022	38.0	38.0	38.0	36.0	38.0
90-91	37.040606545288796	38.0	38.0	38.0	36.0	38.0
92-93	37.03737119667272	38.0	38.0	38.0	36.0	38.0
94-95	37.01678484614874	38.0	38.0	38.0	36.0	38.0
96-97	36.98991379484193	38.0	38.0	38.0	36.0	38.0
98-99	36.955969278828704	38.0	38.0	38.0	35.5	38.0
100	35.63783399909238	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	12.0
21	49.0
22	196.0
23	581.0
24	1510.0
25	3101.0
26	5513.0
27	9177.0
28	14964.0
29	22587.0
30	30090.0
31	38624.0
32	49184.0
33	65981.0
34	98831.0
35	184136.0
36	585784.0
37	2893321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.366993984982685	21.866668531633415	14.408966227283832	22.357371256100063
2	15.43322034575697	20.791029602411957	42.1965634125079	21.579186639323172
3	18.18543094329066	27.645289041922226	30.413152540848788	23.75612747393833
4	13.112472980877929	18.431050097835822	38.50060669699569	29.95587022429056
5	13.250082487374623	38.098776539266865	35.39398992367701	13.257151049681504
6	29.65857277199345	38.44323970591725	18.1413640354328	13.7568234866565
7	26.91491705310609	33.45792809853818	22.615236212192585	17.011918636163138
8	24.822074678930157	37.08756968023646	20.54412403094579	17.546231609887588
9	26.350131854814336	17.26651157469966	21.506796554940173	34.876560015545834
10-11	24.556291939896784	27.237248248221746	28.187606996222943	20.018852815658526
12-13	25.71968151030336	25.276910096447086	28.316564451502458	20.686843941747096
14-15	23.049989934175	26.257004240634664	26.387472818525914	24.305533006664426
16-17	21.682311573001208	29.59909542452055	27.207412943102362	21.511180059375878
18-19	21.78993847108361	28.174781186948096	29.534242038382015	20.50103830358628
20-21	22.681853485629475	27.008290997049002	29.599185424870043	20.71067009245148
22-23	22.64260240537699	26.963945323848115	29.505123422512685	20.888328848262212
24-25	22.29091713559658	27.44838627191568	29.567574313112978	20.693122279374755
26-27	21.829498345419935	27.428780209065746	29.844418367906684	20.897303077607635
28-29	21.545184407883088	26.88569302132027	30.14219098712661	21.42693158367003
30-31	21.99723083455082	26.96991804597867	29.979040260609658	21.05381085886086
32-33	21.626523061606214	27.38080205793379	29.84329413142346	21.149380749036535
34-35	21.823562347862577	27.513700726525197	30.063629305361367	20.59910762025086
36-37	22.025785998753044	27.14910824351796	29.580241328678568	21.244864429050423
38-39	21.687351001272727	27.460827988896703	29.743683452784154	21.108137557046415
40-41	21.768154325592622	27.159982785101068	30.105802810240405	20.966060079065905
42-43	22.08682353985953	27.21547470726668	29.46374233721729	21.233959415656496
44-45	21.672170369872365	27.47769339250502	29.514673364537643	21.33546287308497
46-47	22.251252519998356	27.489235000714118	28.903352131562887	21.356160347724636
48-49	22.022251721497852	27.743363713730723	28.59815727295813	21.63622729181329
50-51	21.827564995194994	27.78404079847887	28.560376297289352	21.828017909036785
52-53	22.05533359785003	27.760120193128657	28.75940188869363	21.425144320327682
54-55	22.145335174695134	27.922327297291005	28.48821503200923	21.444122496004635
56-57	22.129627028773882	28.0374472156326	28.211557957043887	21.62136779854963
58-59	21.799612746904	28.11439960694643	28.6078414875389	21.478146158610667
60-61	22.24599273792912	28.68953739193788	27.984986665578283	21.079483204554723
62-63	21.867252055491665	28.830228229947092	28.21740052807228	21.085119186488967
64-65	21.948297964840833	28.575656122384522	28.165562446352727	21.310483466421918
66-67	22.145130904048653	28.901502402652103	27.790896715161246	21.162469978138006
68-69	21.98723420947356	28.522257254535404	28.19754907445042	21.29295946154061
70-71	22.171870334869144	28.15092282727982	28.359858713788515	21.31734812406252
72-73	22.463753870581318	28.22123817397733	27.92379572609503	21.391212229346323
74-75	21.909543290196996	28.33431464551915	28.140626228962155	21.6155158353217
76-77	22.13029189152228	27.87179653823818	28.46670067286143	21.53121089737811
78-79	22.107276362687994	27.777650681515574	28.360499614680446	21.754573341115982
80-81	22.03368372560863	28.221284860054368	28.077979473591324	21.667051940745672
82-83	22.213591469237493	27.901545315683563	28.271573207714695	21.61329000736425
84-85	22.05046494307905	27.88986062347058	28.247260346845408	21.81241408660496
86-87	22.028737843723835	28.072756976540507	28.403343553501408	21.495161626234246
88-89	22.13331609187368	27.888649294731106	28.300784253421497	21.67725035997372
90-91	22.166182741249905	27.896454021886356	28.337375005627386	21.599988231236356
92-93	22.255187810367026	27.97063335997667	28.189820662646074	21.584358167010226
94-95	22.058293359641056	27.766946915605285	28.393809783882208	21.780949940871455
96-97	22.3777080655615	27.92922970270155	28.217518205081028	21.475544026655918
98-99	23.204672313701046	29.781653300879146	28.4663732783332	18.547301107086607
100	29.802594637166465	40.19550372838153	30.001901634452004	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4250.0
1	3260.5
2	1968.5
3	1752.5
4	2189.0
5	3000.5
6	3766.0
7	4896.5
8	6315.5
9	7299.5
10	7210.5
11	6751.0
12	7252.0
13	9128.5
14	13296.0
15	18256.5
16	21867.0
17	23635.0
18	23250.0
19	21048.0
20	18058.0
21	15164.0
22	13411.0
23	13594.5
24	15495.0
25	19683.0
26	26032.5
27	33274.0
28	41171.5
29	50921.5
30	62315.5
31	73422.5
32	90071.0
33	110882.5
34	124519.0
35	138037.0
36	156717.0
37	167605.0
38	175365.5
39	178647.5
40	178422.0
41	176157.0
42	171333.5
43	176910.5
44	183646.0
45	181888.5
46	172618.5
47	163453.0
48	158831.5
49	154108.0
50	148933.5
51	144983.5
52	140523.0
53	134947.0
54	128431.0
55	120068.0
56	110749.0
57	100853.0
58	90359.0
59	79127.5
60	67397.5
61	55405.0
62	43640.0
63	34415.5
64	28531.5
65	22405.0
66	15092.0
67	10228.5
68	7347.0
69	5330.5
70	3912.5
71	2811.5
72	1995.5
73	1349.0
74	907.5
75	625.0
76	413.0
77	244.5
78	157.0
79	122.0
80	96.0
81	65.0
82	38.0
83	22.5
84	17.0
85	13.0
86	11.5
87	10.0
88	7.0
89	4.5
90	3.5
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00639417171248407
3	0.0040213345535544355
4	0.0
5	7.49316997556727E-5
6	2.747495657707999E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.7488420479589952E-4
26-27	0.0017493271650391468
28-29	0.007226942240727196
30-31	0.002179214328108771
32-33	0.004736521267608678
34-35	0.0028512140620868265
36-37	0.001116967858742152
38-39	2.5610775375188254E-5
40-41	3.880724132774578E-5
42-43	0.0025074007500262493
44-45	5.151233614784411E-4
46-47	3.0786685481347154E-4
48-49	0.0018845316288128887
50-51	4.803608580562486E-4
52-53	0.0
54-55	0.0050211336429418565
56-57	0.05033667436510565
58-59	0.07678285573115441
60-61	0.037967729027439556
62-63	0.016995208878755413
64-65	0.0020213697425622143
66-67	0.0
68-69	0.002442043813623827
70-71	0.004136712940009915
72-73	0.01574012719783909
74-75	0.019452626645832298
76-77	0.025780218398272936
78-79	0.008186144762084653
80-81	0.010107244491758868
82-83	0.03777351985521047
84-85	0.022737673789606058
86-87	0.016393027223284926
88-89	0.008753934246945864
90-91	5.317209663071465E-5
92-93	0.0
94-95	0.0
96-97	7.426282542202171E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	49.0
20-21	211.0
22-23	592.0
24-25	932.0
26-27	1938.0
28-29	4811.0
30-31	11974.0
32-33	15171.0
34-35	21136.0
36-37	32161.0
38-39	40195.0
40-41	36534.0
42-43	40608.0
44-45	49279.0
46-47	48558.0
48-49	45714.0
50-51	43533.0
52-53	36111.0
54-55	38051.0
56-57	40007.0
58-59	42006.0
60-61	40527.0
62-63	39269.0
64-65	41720.0
66-67	44608.0
68-69	47154.0
70-71	50888.0
72-73	39270.0
74-75	39440.0
76-77	40616.0
78-79	41998.0
80-81	42777.0
82-83	38689.0
84-85	37228.0
86-87	38571.0
88-89	40035.0
90-91	40414.0
92-93	38831.0
94-95	42548.0
96-97	106701.0
98-99	820114.0
100-101	1782677.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.3972780378331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.8921514932375	83.04305494573467
2	5.338418071214204	9.54480089189058
3	1.0511540537772899	2.819109336183713
4	0.3435990174069241	1.2286726757061222
5	0.14240104432719558	0.636513287629805
6	0.07487134055674538	0.4015976429289994
7	0.0428138882925799	0.26792115529007454
8	0.02873213999763553	0.20548600863924552
9	0.018901376768210385	0.15207584708209887
>10	0.06176558302047776	0.9010281794255321
>50	0.0029833206373091623	0.1816745781710904
>100	0.0018352730675511636	0.3346258987609122
>500	3.319090633682398E-4	0.21905181855668907
>1k	4.148863292003589E-5	0.06438773400047709
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.49772332518909E-5	0.0	0.0	0.0
3	0.0	2.49772332518909E-5	0.0	0.0	0.0
4	0.0	4.99544665037818E-5	0.0	2.49772332518909E-5	0.0
5	0.0	4.99544665037818E-5	0.0	2.49772332518909E-5	0.0
6	0.0	4.99544665037818E-5	0.0	2.49772332518909E-5	0.0
7	0.0	7.493169975567271E-5	0.0	2.49772332518909E-5	0.0
8	0.0	9.99089330075636E-5	0.0	2.49772332518909E-5	0.0
9	0.0	2.247950992670181E-4	0.0	2.49772332518909E-5	0.0
10-11	0.0	2.247950992670181E-4	0.0	4.99544665037818E-5	0.0
12-13	0.0	2.247950992670181E-4	0.0	4.99544665037818E-5	0.0
14-15	0.0	3.247040322745817E-4	0.0	6.244308312972725E-5	0.0
16-17	0.0	3.247040322745817E-4	0.0	7.493169975567271E-5	0.0
18-19	0.0	3.3719264890052716E-4	0.0	9.99089330075636E-5	0.0
20-21	0.0	4.1212434865619987E-4	0.0	9.99089330075636E-5	0.0
22-23	0.0	4.6207881515998166E-4	0.0	9.99089330075636E-5	0.0
24-25	0.0	5.120332816637634E-4	0.0	1.748406327632363E-4	0.0
26-27	0.0	5.245218982897089E-4	0.0	3.6216988215241803E-4	0.0
28-29	2.49772332518909E-5	5.370105149156544E-4	0.0	8.117600806864543E-4	0.0
30-31	2.49772332518909E-5	6.11942214671327E-4	0.0	0.003022245223478799	0.0
32-33	2.49772332518909E-5	6.244308312972725E-4	0.0	0.007280863492926198	0.0
34-35	2.49772332518909E-5	7.243397643048361E-4	0.0	0.012413684926189779	0.0
36-37	2.49772332518909E-5	8.367373139383452E-4	0.0	0.021667749846015355	0.0
38-39	2.49772332518909E-5	8.492259305642907E-4	0.0	0.0415995819810243	0.0
40-41	2.49772332518909E-5	9.241576303199633E-4	0.0	0.0639417171248407	0.0
42-43	2.49772332518909E-5	9.366462469459088E-4	0.0	0.07645531098403804	0.0
44-45	2.49772332518909E-5	0.0010740210298313087	0.0	0.09134174200216502	0.0
46-47	2.49772332518909E-5	0.0011989071960907633	0.0	0.10773929563203141	0.0
48-49	2.49772332518909E-5	0.0011989071960907633	0.0	0.12283803313279945	0.0
50-51	2.49772332518909E-5	0.0013987250621058905	0.0	0.13952282494506257	0.0
52-53	2.49772332518909E-5	0.0013987250621058905	0.0	0.17854975190114208	0.0
54-55	2.49772332518909E-5	0.0013987250621058905	0.0	0.22320904495552302	0.0
56-57	2.49772332518909E-5	0.0014237022953577814	0.0	0.2767477444309512	0.0
58-59	2.49772332518909E-5	0.0014237022953577814	0.0	0.3184847011948609	0.0
60-61	2.49772332518909E-5	0.0014237022953577814	0.0	0.3345325735592008	0.0
62-63	2.49772332518909E-5	0.0014486795286096723	0.0	0.3516294897201201	2.49772332518909E-5
64-65	2.49772332518909E-5	0.0016235201613729086	0.0	0.36042147582478573	2.49772332518909E-5
66-67	2.49772332518909E-5	0.0016235201613729086	0.0	0.3675899417680784	3.7465849877836354E-5
68-69	2.49772332518909E-5	0.0017109404777545267	0.0	0.3749707141940122	4.99544665037818E-5
70-71	2.49772332518909E-5	0.001773383560884254	0.0	0.38195185088791567	4.99544665037818E-5
72-73	2.49772332518909E-5	0.001773383560884254	0.0	0.3872969788038203	4.99544665037818E-5
74-75	2.49772332518909E-5	0.0018233380273880357	0.0	0.39139324505713047	4.99544665037818E-5
76-77	2.49772332518909E-5	0.0018982697271437085	0.0	0.3949899666454027	4.99544665037818E-5
78-79	2.49772332518909E-5	0.0019232469603955994	0.0	0.3971504973216913	4.99544665037818E-5
80-81	2.49772332518909E-5	0.0019732014268993812	0.0	0.3979372801691259	4.99544665037818E-5
82-83	2.49772332518909E-5	0.0019856900435253266	0.0	0.3986116654669269	4.99544665037818E-5
84-85	2.49772332518909E-5	0.001998178660151272	0.0	0.398823971949568	4.99544665037818E-5
86-87	2.49772332518909E-5	0.0020356445100291084	0.0	0.3989238808825755	4.99544665037818E-5
88	2.49772332518909E-5	0.0021230648264107268	0.0	0.39896134673245337	4.99544665037818E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8330	0.0	55.1388	1
GTATCAA	15775	0.0	36.45998	1
TGGTATC	3370	0.0	30.85413	2
TCAACGC	19275	0.0	29.327215	4
ATCAACG	19575	0.0	28.878836	3
TATCAAC	19750	0.0	28.74874	2
CAACGCA	19845	0.0	28.48486	5
AACGCAG	20520	0.0	27.587753	6
GTGGTAT	4000	0.0	26.91567	1
ACCGAAC	1235	0.0	25.520859	8
GGACCGA	1285	0.0	24.527826	6
AGGACCG	1370	0.0	24.499926	5
ACGCAGA	23215	0.0	24.349861	7
CGCAGAG	23460	0.0	24.113016	8
GACCGAA	1325	0.0	22.860586	7
CCGAACC	1375	0.0	22.624678	9
TTAGCCG	615	0.0	22.60321	94
TGCGTAT	715	0.0	22.034176	94
GCAGAGT	26155	0.0	21.769272	9
GAGTACT	13120	0.0	21.246372	12-13
>>END_MODULE