FastQCFastQC Report
Fri 27 May 2016
SRR2139602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8526854
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129450.052.7062951
GTATCAA237700.036.992541
TCAACGC292450.029.7224184
ATCAACG293400.029.6407873
TATCAAC298350.029.206122
CAACGCA298150.029.1541885
AACGCAG308900.028.1937626
ACGCAGA353100.024.6408587
CGCAGAG359650.024.1920978
TGGTATC55500.023.2903542
GTGGTAT60850.022.3425831
GCAGAGT413300.021.1428369
GAGTACT231250.019.80717812-13
GTACATG324400.019.5878851
TACATGG319350.019.5439592
CAGAGTA402400.019.27267510-11
ACATGGG322650.018.7470073
GTACTTT249850.018.2656714-15
AGAGTAC383800.017.74351510-11
AGTACTT253550.016.67929812-13
Produced by FastQC (version 0.11.5)