##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139602_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8526854
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.95335102489148	32.0	18.0	33.0	18.0	33.0
2	29.547078676379353	31.0	27.0	33.0	25.0	33.0
3	31.966207349158317	33.0	31.0	33.0	29.0	34.0
4	31.860845043201163	33.0	31.0	33.0	29.0	34.0
5	32.84566969247978	33.0	33.0	34.0	32.0	34.0
6	36.832107597948784	38.0	37.0	38.0	35.0	38.0
7	37.000514726767925	38.0	38.0	38.0	35.0	38.0
8	37.134180320197814	38.0	38.0	38.0	36.0	38.0
9	37.216698210148785	38.0	38.0	38.0	36.0	38.0
10-11	37.329807218465334	38.0	38.0	38.0	36.5	38.0
12-13	37.3859063964271	38.0	38.0	38.0	37.0	38.0
14-15	37.393423940412255	38.0	38.0	38.0	37.0	38.0
16-17	37.44601578729975	38.0	38.0	38.0	37.0	38.0
18-19	37.436211467910674	38.0	38.0	38.0	37.0	38.0
20-21	37.44816710916895	38.0	38.0	38.0	37.0	38.0
22-23	37.45820450097328	38.0	38.0	38.0	37.0	38.0
24-25	37.473075147925854	38.0	38.0	38.0	37.0	38.0
26-27	37.433035121131084	38.0	38.0	38.0	37.0	38.0
28-29	37.40100948130284	38.0	38.0	38.0	37.0	38.0
30-31	37.41764125568392	38.0	38.0	38.0	37.0	38.0
32-33	37.409669601977924	38.0	38.0	38.0	37.0	38.0
34-35	37.379055945977285	38.0	38.0	38.0	37.0	38.0
36-37	37.35793378182397	38.0	38.0	38.0	37.0	38.0
38-39	37.36261945031332	38.0	38.0	38.0	37.0	38.0
40-41	37.36459951495719	38.0	38.0	38.0	37.0	38.0
42-43	37.330955583754445	38.0	38.0	38.0	37.0	38.0
44-45	37.2952291396038	38.0	38.0	38.0	37.0	38.0
46-47	37.27373714640845	38.0	38.0	38.0	37.0	38.0
48-49	37.218480486250655	38.0	38.0	38.0	37.0	38.0
50-51	37.17771428391596	38.0	38.0	38.0	37.0	38.0
52-53	37.167224215616955	38.0	38.0	38.0	37.0	38.0
54-55	37.15373876293464	38.0	38.0	38.0	37.0	38.0
56-57	37.109943626051226	38.0	38.0	38.0	36.0	38.0
58-59	37.093783710753144	38.0	38.0	38.0	36.0	38.0
60-61	37.11969936660575	38.0	38.0	38.0	36.0	38.0
62-63	37.13329293803993	38.0	38.0	38.0	36.0	38.0
64-65	37.142668838102296	38.0	38.0	38.0	36.0	38.0
66-67	37.14738298698259	38.0	38.0	38.0	36.0	38.0
68-69	37.14085137595625	38.0	38.0	38.0	36.0	38.0
70-71	37.13909069624913	38.0	38.0	38.0	36.0	38.0
72-73	37.13803958577343	38.0	38.0	38.0	36.0	38.0
74-75	37.12387559949891	38.0	38.0	38.0	36.0	38.0
76-77	37.108824749211536	38.0	38.0	38.0	36.0	38.0
78-79	37.11429464141453	38.0	38.0	38.0	36.0	38.0
80-81	37.11626877308339	38.0	38.0	38.0	36.0	38.0
82-83	37.075545846728446	38.0	38.0	38.0	36.0	38.0
84-85	37.061205639279976	38.0	38.0	38.0	36.0	38.0
86-87	37.06562217782955	38.0	38.0	38.0	36.0	38.0
88-89	37.054436601490615	38.0	38.0	38.0	36.0	38.0
90-91	37.03444848831823	38.0	38.0	38.0	36.0	38.0
92-93	37.02602503136255	38.0	38.0	38.0	36.0	38.0
94-95	37.011633306897906	38.0	38.0	38.0	36.0	38.0
96-97	36.98807037037865	38.0	38.0	38.0	36.0	38.0
98-99	36.95903086920816	38.0	38.0	38.0	35.5	38.0
100	35.655372850611116	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	6.0
20	28.0
21	109.0
22	362.0
23	1222.0
24	3128.0
25	6460.0
26	11975.0
27	20339.0
28	32709.0
29	48079.0
30	65033.0
31	83986.0
32	108253.0
33	145354.0
34	218643.0
35	411450.0
36	1266706.0
37	6103009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19665940099361	21.261827633028545	14.085746044203407	23.455766921774433
2	15.222316647436292	20.571675735318436	42.12272833146581	22.08327928577946
3	18.418109191497656	26.45155252251704	30.13729010690499	24.993048179080315
4	12.885854501554736	17.817028413996532	38.752557508314325	30.54455957613441
5	13.524734981296143	37.565755517482366	35.44504295900093	13.464466542220569
6	30.185704395923878	37.91552805491278	18.423186761881407	13.475580787281935
7	26.773156899367574	32.876580272161334	22.9546207780736	17.395642050397488
8	24.276280560215994	37.36073116767333	20.79748287000106	17.56550540210962
9	25.80095777410989	17.1152220971533	21.477874489231315	35.6059456395055
10-11	24.412321355566778	27.034273132857678	28.63931410107409	19.91409141050146
12-13	25.200824360309205	24.666623821634566	28.836450113957625	21.296101704098604
14-15	22.423821259282732	26.004766822558473	26.701577158468996	24.869834759689798
16-17	21.705895280955907	28.978642064236116	27.473978093209993	21.841484561597984
18-19	21.686779203678167	28.04568953567166	29.356665424317104	20.910865836333073
20-21	22.72420750918726	27.153852784558723	29.22661082674476	20.89532887950926
22-23	22.39869064876454	26.946909349358926	29.12480237756792	21.529597624308614
24-25	22.028725473171306	27.168844890620214	29.30191093605482	21.50051870015366
26-27	21.892505995435926	27.27503082034738	29.236652522564345	21.595810661652358
28-29	21.850883631969367	27.32146636847955	29.31837374607576	21.509276253475328
30-31	22.176172700608323	27.226527203333706	29.132788231333155	21.464511864724813
32-33	21.89300491024333	27.425128757904044	29.07531731461692	21.60654901723571
34-35	22.06070662244231	27.392261935402047	29.120631066426306	21.426400375729337
36-37	22.08492218075043	27.19707864894938	29.08672738659705	21.63127178370314
38-39	21.847313816510855	27.460039523743202	29.179242185589295	21.51340447415665
40-41	22.123000575022665	27.32296041088038	29.06294824705531	21.491090767041644
42-43	22.06566810913022	27.400692107738394	28.780378799718836	21.753260983412552
44-45	22.05948223453162	27.567327206776564	28.68032735182885	21.69286320686296
46-47	22.17638719643521	27.507000695464335	28.417622953387017	21.89898915471343
48-49	22.132951951277246	27.589455690539904	28.36519220893331	21.91240014924954
50-51	22.08879833972085	27.741086368392644	28.226569215499104	21.943546076387406
52-53	22.276814378020756	27.682664927216777	28.115904014782767	21.924616679979696
54-55	22.31068384942974	27.613839805125938	28.09201901594896	21.98345732949536
56-57	22.222264962436135	27.76339171153954	28.082621991291774	21.931721334732547
58-59	22.183379842713734	27.746608988445686	28.148324838644847	21.921686330195737
60-61	22.262029684099343	27.796486952114453	28.12289039307634	21.818592970709872
62-63	22.20603703845319	27.834171312505806	28.149131688447675	21.81065996059333
64-65	22.20753760557961	27.759992019820164	28.13041215321674	21.902058221383488
66-67	22.35401186422232	27.945912817722967	27.839385396992945	21.86068992106177
68-69	22.278799672924517	27.841572258939934	28.046563044686636	21.833065023448917
70-71	22.299895899144573	27.468954748429248	28.204492771545514	22.02665658088067
72-73	22.37148454965724	27.661434252451638	28.021097259826284	21.94598393806484
74-75	22.086347792556165	27.78908013266847	28.048312512287637	22.076259562487728
76-77	22.24706586169021	27.652416006074226	28.05239312330831	22.04812500892725
78-79	22.255377617606975	27.671498855893145	27.95701763979011	22.116105886709768
80-81	22.241021291356468	27.723362936670004	27.869753634189713	22.16586213778381
82-83	22.357356016629446	27.602492432687903	28.0064807699477	22.03367078073495
84-85	22.41813891103814	27.583803027950317	27.858722304472778	22.139335756538767
86-87	22.216733579676525	27.71191791081663	27.976756444408164	22.094592065098684
88-89	22.35010428674409	27.67017898519232	27.90182445211246	22.077892275951132
90-91	22.368915865331974	27.710911705853913	27.90679410300009	22.013378325814017
92-93	22.415012339344006	27.720834528016702	27.833497756867292	22.030655375771996
94-95	22.237264834486727	27.728155923531634	27.89655901389279	22.138020228088852
96-97	22.383347633529834	27.839876817192195	27.937054584773424	21.83972096450455
98-99	23.415819506490724	29.718271731029393	28.11137064244938	18.75453812003051
100	29.889046790963114	40.71018949831537	29.400763710721513	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1894.0
1	1730.5
2	2002.5
3	2667.0
4	3551.5
5	5181.0
6	6580.5
7	8059.0
8	9795.0
9	10810.0
10	10987.0
11	11172.5
12	12472.0
13	15544.5
14	22083.5
15	30049.5
16	35708.0
17	38287.5
18	37822.0
19	35141.5
20	31627.5
21	28762.0
22	28995.0
23	32902.5
24	40354.5
25	52232.0
26	69967.0
27	89195.5
28	105337.0
29	124114.0
30	142243.5
31	159038.5
32	183767.5
33	212362.0
34	236367.0
35	259517.5
36	287102.5
37	307122.5
38	318977.5
39	328045.0
40	338797.5
41	347209.5
42	348242.0
43	352457.0
44	359530.0
45	363370.0
46	361474.5
47	359947.0
48	359317.0
49	355902.0
50	349256.0
51	338626.5
52	323952.0
53	306123.0
54	286746.5
55	266432.0
56	244158.5
57	221119.0
58	197752.0
59	171608.0
60	144905.5
61	116980.5
62	90528.0
63	69298.0
64	51976.0
65	38494.0
66	28008.0
67	21061.0
68	15798.0
69	11599.0
70	8561.0
71	6031.0
72	4195.0
73	2885.5
74	1974.0
75	1291.5
76	831.0
77	555.5
78	367.5
79	222.0
80	143.0
81	99.5
82	77.5
83	51.5
84	32.5
85	23.0
86	17.5
87	12.0
88	7.5
89	7.0
90	6.0
91	4.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004831793765907098
3	0.0036473006339735616
4	0.0
5	3.518296431485751E-5
6	2.6973605974724087E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.1082619361656836E-4
26-27	0.0021760586275944294
28-29	0.008519800291656469
30-31	0.002778677568012099
32-33	0.005760002600835531
34-35	0.0031971667311221784
36-37	0.0012115727288994958
38-39	2.3930572622707006E-5
40-41	6.030648358020295E-5
42-43	0.00261386115984071
44-45	6.135845659429151E-4
46-47	3.6593459074247506E-4
48-49	0.0018931664839322195
50-51	4.7490596861821365E-4
52-53	0.0
54-55	0.0053994008406809055
56-57	0.05206523023974638
58-59	0.07635102667637449
60-61	0.0376991654494433
62-63	0.016239291776065205
64-65	0.0016489385416176819
66-67	0.0
68-69	0.002350661080090629
70-71	0.004496034511588967
72-73	0.015986880261032985
74-75	0.019333828113020528
76-77	0.025624160328522758
78-79	0.007216847113514185
80-81	0.008945834051289101
82-83	0.03627505931888843
84-85	0.02092751761114438
86-87	0.015633268425294077
88-89	0.007855990515563767
90-91	5.514038782125282E-5
92-93	0.0
94-95	0.0
96-97	4.1100375419049156E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	58.0
20-21	302.0
22-23	674.0
24-25	855.0
26-27	2364.0
28-29	6499.0
30-31	20509.0
32-33	26792.0
34-35	36604.0
36-37	57521.0
38-39	67833.0
40-41	64400.0
42-43	72596.0
44-45	85947.0
46-47	87040.0
48-49	81696.0
50-51	74501.0
52-53	71026.0
54-55	76712.0
56-57	79319.0
58-59	78220.0
60-61	75445.0
62-63	72956.0
64-65	75997.0
66-67	80007.0
68-69	81770.0
70-71	80757.0
72-73	74928.0
74-75	76110.0
76-77	79605.0
78-79	82175.0
80-81	81746.0
82-83	75580.0
84-85	74153.0
86-87	76251.0
88-89	80074.0
90-91	80937.0
92-93	78776.0
94-95	88536.0
96-97	248821.0
98-99	1868985.0
100-101	4001777.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.41720506893502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.3157564304725	77.14519487977657
2	6.749240504098012	11.530025203962042
3	1.6232673324238007	4.159648758460406
4	0.5915984875232195	2.0213075730897057
5	0.2704556004560924	1.15507807431
6	0.15470614337155297	0.79287398242752
7	0.07644061900641243	0.45705408204869435
8	0.053874999670973256	0.3681481515987463
9	0.03650959944383553	0.2806693148410898
>10	0.12410471907484218	1.6850623014168693
>50	0.0030521385596578004	0.17476172985401014
>100	8.791475415592921E-4	0.1286581492617269
>500	6.856713432119248E-5	0.03881112863637075
>1k	4.571122320309085E-5	0.06270667031622076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.172765477161917E-5	1.172765477161917E-5
2	0.0	0.0	0.0	2.345530954323834E-5	1.172765477161917E-5
3	0.0	0.0	0.0	3.518296431485751E-5	1.172765477161917E-5
4	0.0	2.345530954323834E-5	0.0	3.518296431485751E-5	2.345530954323834E-5
5	0.0	2.345530954323834E-5	0.0	3.518296431485751E-5	2.345530954323834E-5
6	0.0	2.345530954323834E-5	0.0	3.518296431485751E-5	4.691061908647668E-5
7	0.0	2.345530954323834E-5	0.0	3.518296431485751E-5	4.691061908647668E-5
8	0.0	2.345530954323834E-5	0.0	4.691061908647668E-5	5.8638273858095845E-5
9	0.0	8.209358340133419E-5	0.0	1.0554889294457252E-4	5.8638273858095845E-5
10-11	0.0	8.209358340133419E-5	0.0	1.114127203303821E-4	6.450210124390543E-5
12-13	0.0	8.209358340133419E-5	0.0	1.1727654771619169E-4	7.036592862971502E-5
14-15	0.0	1.4073185725943003E-4	0.0	1.1727654771619169E-4	1.0554889294457252E-4
16-17	0.0	1.524595120310492E-4	0.0	1.2900420248781086E-4	1.1727654771619169E-4
18-19	0.0	1.583233394168588E-4	0.0	1.4073185725943003E-4	1.7005099418847795E-4
20-21	0.0	1.6418716680266838E-4	0.0	1.4073185725943003E-4	1.8764247634590672E-4
22-23	0.0	1.7591482157428755E-4	0.0	1.465956846452396E-4	2.0523395850333546E-4
24-25	0.0	1.935063037317163E-4	0.0	1.524595120310492E-4	2.228254406607642E-4
26-27	0.0	1.9937013111752586E-4	0.0	1.993701311175259E-4	2.228254406607642E-4
28-29	0.0	1.9937013111752586E-4	0.0	4.92561500408005E-4	2.2868926804657378E-4
30-31	0.0	2.580084049756217E-4	0.0	0.0015070036381530632	2.4041692281819298E-4
32-33	0.0	2.580084049756217E-4	0.0	0.0036473006339735616	2.462807502040026E-4
34-35	0.0	3.1664667883371755E-4	0.0	0.006514712225634449	2.462807502040026E-4
36-37	0.0	3.6355729792019424E-4	0.0	0.010947765729306495	2.462807502040026E-4
38-39	0.0	3.7528495269181344E-4	0.0	0.017872945871947613	2.7559988713305047E-4
40-41	0.0	4.163317443924805E-4	0.0	0.025185138622052166	2.8146371451886007E-4
42-43	0.0	4.2805939916409967E-4	0.0	0.031963723080048044	2.8732754190466967E-4
44-45	0.0	4.51514708707338E-4	0.0	0.03910586483596412	2.931913692904792E-4
46-47	0.0	4.7497001825057636E-4	0.0	0.04716276366406649	2.931913692904792E-4
48-49	0.0	4.8669767302219556E-4	0.0	0.05498510939673648	2.931913692904792E-4
50-51	0.0	5.336082921086722E-4	0.0	0.06277813599247742	2.931913692904792E-4
52-53	0.0	5.570636016519106E-4	0.0	0.07059461789776159	3.049190240620984E-4
54-55	0.0	5.746550838093393E-4	0.0	0.07848732955906129	3.049190240620984E-4
56-57	0.0	5.863827385809584E-4	0.0	0.08604580305936985	3.2251050621952715E-4
58-59	0.0	5.863827385809584E-4	0.0	0.0941554763339445	3.401019883769559E-4
60-61	0.0	6.157018755100065E-4	0.0	0.10142075846496257	3.518296431485751E-4
62-63	0.0	6.274295302816256E-4	0.0	0.10843389601839085	3.518296431485751E-4
64-65	0.0	7.388422506120077E-4	0.0	0.11477855724983682	3.518296431485751E-4
66-67	0.0	7.388422506120077E-4	0.0	0.12163337146384821	3.5769347053438464E-4
68-69	0.0	7.505699053836268E-4	0.0	0.12830640702889953	3.6355729792019424E-4
70-71	0.0	7.681613875410555E-4	0.0	0.13448688109354284	3.8114878007762304E-4
72-73	0.0	7.740252149268652E-4	0.0	0.13893752607937232	4.573785360931476E-4
74-75	0.0	7.916166970842939E-4	0.0	0.1424206395465432	4.573785360931476E-4
76-77	0.0	8.502549709423898E-4	0.0	0.1458392509124702	4.573785360931476E-4
78-79	0.0	8.795741078714377E-4	0.0	0.14807336914646363	4.573785360931476E-4
80-81	0.0	8.795741078714377E-4	0.0	0.14875943695060334	4.6910619086476676E-4
82-83	0.0	8.795741078714377E-4	0.0	0.14931650055225526	4.925615004080052E-4
84-85	0.0	8.795741078714377E-4	0.0	0.14953932599291603	4.925615004080052E-4
86-87	0.0	8.795741078714377E-4	0.0	0.1496448748858606	4.984253277938147E-4
88	0.0	8.795741078714377E-4	0.0	0.1496566025406322	5.042891551796242E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12945	0.0	52.706295	1
GTATCAA	23770	0.0	36.99254	1
TCAACGC	29245	0.0	29.722418	4
ATCAACG	29340	0.0	29.640787	3
TATCAAC	29835	0.0	29.20612	2
CAACGCA	29815	0.0	29.154188	5
AACGCAG	30890	0.0	28.193762	6
ACGCAGA	35310	0.0	24.640858	7
CGCAGAG	35965	0.0	24.192097	8
TGGTATC	5550	0.0	23.290354	2
GTGGTAT	6085	0.0	22.342583	1
GCAGAGT	41330	0.0	21.142836	9
GAGTACT	23125	0.0	19.807178	12-13
GTACATG	32440	0.0	19.587885	1
TACATGG	31935	0.0	19.543959	2
CAGAGTA	40240	0.0	19.272675	10-11
ACATGGG	32265	0.0	18.747007	3
GTACTTT	24985	0.0	18.26567	14-15
AGAGTAC	38380	0.0	17.743515	10-11
AGTACTT	25355	0.0	16.679298	12-13
>>END_MODULE