FastQCFastQC Report
Fri 27 May 2016
SRR2139601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6569957
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116650.052.7698141
GTATCAA218850.036.9620361
ATCAACG266050.029.901163
TCAACGC267100.029.8298364
TATCAAC270000.029.5885732
CAACGCA273000.029.154575
TGGTATC49000.028.4657882
AACGCAG282950.028.1293416
GTGGTAT51750.027.43731
ACGCAGA322650.024.6682077
CGCAGAG325500.024.439398
GCAGAGT369000.021.6149089
GAGTACT198750.020.32779312-13
CAGAGTA368250.019.07913610-11
TACATGG284100.018.6977212
GTACATG289350.018.6182581
GTACTTT217650.018.4478314-15
ACATGGG285100.018.133423
AGAGTAC347550.017.71633310-11
AGTACTT215450.016.69764312-13
Produced by FastQC (version 0.11.5)