##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139601_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6569957
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.216504765556305	32.0	25.0	33.0	18.0	33.0
2	29.56592988355936	31.0	27.0	33.0	25.0	33.0
3	32.003144465024654	33.0	31.0	33.0	29.0	34.0
4	31.863499867655147	33.0	31.0	33.0	29.0	34.0
5	32.86195267335844	33.0	33.0	34.0	32.0	34.0
6	36.85996879431631	38.0	37.0	38.0	35.0	38.0
7	37.02815710970407	38.0	38.0	38.0	35.0	38.0
8	37.15324544742074	38.0	38.0	38.0	36.0	38.0
9	37.228915044649455	38.0	38.0	38.0	36.0	38.0
10-11	37.344733078161696	38.0	38.0	38.0	36.5	38.0
12-13	37.40157804685784	38.0	38.0	38.0	37.0	38.0
14-15	37.409277945045915	38.0	38.0	38.0	37.0	38.0
16-17	37.46572298722808	38.0	38.0	38.0	37.0	38.0
18-19	37.45422306416922	38.0	38.0	38.0	37.0	38.0
20-21	37.469196834774195	38.0	38.0	38.0	37.0	38.0
22-23	37.47462841635816	38.0	38.0	38.0	37.0	38.0
24-25	37.48796797719248	38.0	38.0	38.0	37.0	38.0
26-27	37.448308639708856	38.0	38.0	38.0	37.0	38.0
28-29	37.417207929493586	38.0	38.0	38.0	37.0	38.0
30-31	37.43204312584504	38.0	38.0	38.0	37.0	38.0
32-33	37.42311284791441	38.0	38.0	38.0	37.0	38.0
34-35	37.38993575916487	38.0	38.0	38.0	37.0	38.0
36-37	37.36589834424211	38.0	38.0	38.0	37.0	38.0
38-39	37.36712059902996	38.0	38.0	38.0	37.0	38.0
40-41	37.36649174096931	38.0	38.0	38.0	37.0	38.0
42-43	37.32646116226875	38.0	38.0	38.0	37.0	38.0
44-45	37.28556886368689	38.0	38.0	38.0	37.0	38.0
46-47	37.25112727242591	38.0	38.0	38.0	37.0	38.0
48-49	37.18893799535918	38.0	38.0	38.0	37.0	38.0
50-51	37.144903306264425	38.0	38.0	38.0	37.0	38.0
52-53	37.13387761059528	38.0	38.0	38.0	37.0	38.0
54-55	37.12127565382716	38.0	38.0	38.0	36.5	38.0
56-57	37.07751485241572	38.0	38.0	38.0	36.0	38.0
58-59	37.0641318047636	38.0	38.0	38.0	36.0	38.0
60-61	37.089422604307515	38.0	38.0	38.0	36.0	38.0
62-63	37.10495043332148	38.0	38.0	38.0	36.0	38.0
64-65	37.11826975976339	38.0	38.0	38.0	36.0	38.0
66-67	37.12228003100935	38.0	38.0	38.0	36.0	38.0
68-69	37.1189409758002	38.0	38.0	38.0	36.0	38.0
70-71	37.11850085262829	38.0	38.0	38.0	36.0	38.0
72-73	37.120432284509135	38.0	38.0	38.0	36.0	38.0
74-75	37.1113534566515	38.0	38.0	38.0	36.0	38.0
76-77	37.09938622215071	38.0	38.0	38.0	36.0	38.0
78-79	37.10802970990663	38.0	38.0	38.0	36.0	38.0
80-81	37.11198085815695	38.0	38.0	38.0	36.0	38.0
82-83	37.07405454810572	38.0	38.0	38.0	36.0	38.0
84-85	37.063834441945644	38.0	38.0	38.0	36.0	38.0
86-87	37.07021517329206	38.0	38.0	38.0	36.0	38.0
88-89	37.06173016726171	38.0	38.0	38.0	36.0	38.0
90-91	37.043709093869836	38.0	38.0	38.0	36.0	38.0
92-93	37.039368214583234	38.0	38.0	38.0	36.0	38.0
94-95	37.02551185915359	38.0	38.0	38.0	36.0	38.0
96-97	37.00684575605678	38.0	38.0	38.0	36.0	38.0
98-99	36.97604698781221	38.0	38.0	38.0	35.5	38.0
100	35.683421773165755	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	7.0
20	30.0
21	88.0
22	287.0
23	903.0
24	2430.0
25	5019.0
26	9217.0
27	15869.0
28	26127.0
29	39265.0
30	52694.0
31	65954.0
32	84186.0
33	111894.0
34	166940.0
35	308887.0
36	959193.0
37	4720964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06249097216313	21.75478469645996	14.454691255970168	22.728033075406735
2	14.838661950413584	21.054894786945123	42.53532395014508	21.571119312496215
3	18.100418844390106	27.303275029175417	30.676892535978954	23.91941359045552
4	12.627464685080891	18.390439998313536	39.31450388488083	29.667591431724745
5	13.055540419308873	38.066217815223666	35.706292616356215	13.171949149111242
6	29.130025010898127	38.52402824015333	18.919849447544085	13.426097301404457
7	25.972163896963103	33.71551746837917	23.283074759850024	17.029243874807705
8	23.980065622956133	37.46546590791994	21.156531161467267	17.397937307656655
9	25.678098045390556	17.40242744358905	21.903826767815985	35.01564774320441
10-11	23.88397671400285	27.549023532421902	28.869374639742695	19.697625113832558
12-13	24.901707880279886	25.26536322840469	29.230708815902446	20.602220075412976
14-15	22.05319608636708	26.518369907139423	27.305186320093117	24.123247686400383
16-17	20.98821955760137	29.716450198989126	28.064978203053688	21.23035204035582
18-19	20.949490232584473	28.53563577356747	30.28442956323763	20.23044443061043
20-21	22.04935944864805	27.511736503230573	30.222194080354807	20.21670996776657
22-23	21.80064058577584	27.41422172268317	30.085840685873077	20.699297005667916
24-25	21.35666942376284	27.631865475830473	30.23738605666415	20.774079043742535
26-27	21.24286137437827	27.733901680307564	30.203887651036947	20.819349294277217
28-29	21.127727278854817	27.770591934505934	30.25907192720661	20.842608859432644
30-31	21.400039619705723	27.685497006943212	30.14204838758625	20.772414985764815
32-33	21.166295698721278	27.81542500388933	30.115854378740497	20.90242491864889
34-35	21.34866230266849	27.826525472654385	30.109424183292223	20.7153880413849
36-37	21.320545517331933	27.70906945038641	30.071207699828385	20.899177332453274
38-39	21.17076576956192	27.85311193038367	30.163364742808785	20.81275755724562
40-41	21.387505142234158	27.761636169257525	30.041765824809662	20.809092863698652
42-43	21.3557106758859	27.826123953198255	29.777051712471824	21.04111365844402
44-45	21.373997553751394	28.001066109127297	29.586473039059797	21.038463298061508
46-47	21.508422555380918	28.016981737836744	29.29003171279651	21.18456399398583
48-49	21.498864619994098	28.11522843715309	29.178019108290638	21.20788783456217
50-51	21.4757126108619	28.253837262118164	29.01318132944923	21.257268797570706
52-53	21.604063670534988	28.2665770265494	28.89725611279556	21.232103190120057
54-55	21.628944137373708	28.265751285125447	28.846865354844365	21.258439222656484
56-57	21.615861094532935	28.37038290710099	28.7311607933262	21.28259520503988
58-59	21.548298635270218	28.331896972768266	28.885521133882385	21.23428325807913
60-61	21.648288609191344	28.44115620911312	28.799966521550065	21.110588660145474
62-63	21.54615430930612	28.48518453952458	28.868304373121507	21.10035677804779
64-65	21.564954641654086	28.441449419891025	28.821185077555437	21.17241086089945
66-67	21.64644501105091	28.548709565438056	28.64321082689944	21.161634596611588
68-69	21.641605115680658	28.398496740865347	28.78283633307756	21.177061810376436
70-71	21.625561983511155	28.098201039687225	28.944137605974262	21.332099370827358
72-73	21.735759269781745	28.23960823592077	28.797987988232705	21.22664450606478
74-75	21.53115392379482	28.37680766987968	28.759923388091973	21.33211501823353
76-77	21.616780220389092	28.193115362594472	28.80720050728608	21.382903909730356
78-79	21.573494682208292	28.192223039949145	28.72889067983652	21.505391598006042
80-81	21.57527950592713	28.288731258285782	28.656552536009972	21.479436699777118
82-83	21.70251969265577	28.18203551657415	28.718385880504414	21.39705891026566
84-85	21.764791580584546	28.197901610065035	28.59194266396375	21.44536414538667
86-87	21.56388021766862	28.265519734782902	28.709634221787006	21.460965825761477
88-89	21.69245576744878	28.215527732225926	28.62361507960316	21.468401420722135
90-91	21.762068877981168	28.23699206632086	28.57645554149151	21.424483514206468
92-93	21.77020367284383	28.275429833126687	28.53828510016372	21.416081393865767
94-95	21.626822810858126	28.293547379842455	28.584834874594172	21.49479493470525
96-97	21.737508489736832	28.426887727937917	28.602765227029277	21.232838555295974
98-99	22.744031191010965	30.32310195376691	28.829471344201224	18.103395511020906
100	28.668364227911848	41.10622752846826	30.2254082436199	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1980.0
1	1672.0
2	1692.0
3	2224.0
4	2960.5
5	4183.0
6	5252.0
7	6746.5
8	8547.0
9	9663.0
10	10006.0
11	10352.5
12	11642.0
13	14719.5
14	20953.5
15	28085.5
16	33411.0
17	36197.0
18	35898.0
19	33485.5
20	30418.5
21	27851.5
22	27541.0
23	31063.5
24	38087.5
25	48259.0
26	62156.5
27	77689.5
28	93727.5
29	111901.5
30	129757.5
31	146791.0
32	167035.5
33	187919.0
34	206065.5
35	223717.0
36	243159.0
37	257512.0
38	266662.5
39	273628.5
40	278571.0
41	281702.5
42	282432.5
43	284387.5
44	286631.0
45	287317.5
46	282524.0
47	275835.0
48	269979.5
49	260900.5
50	250721.0
51	238726.5
52	224132.0
53	208525.5
54	190857.0
55	172750.0
56	154337.0
57	136217.0
58	119699.0
59	102647.5
60	85879.5
61	69551.5
62	53939.0
63	41581.0
64	32101.0
65	24106.5
66	17439.0
67	13052.5
68	9960.0
69	7351.0
70	5367.0
71	3792.5
72	2658.0
73	1864.5
74	1331.0
75	953.0
76	645.5
77	394.0
78	257.0
79	177.0
80	118.5
81	78.5
82	49.0
83	33.5
84	29.5
85	25.5
86	17.0
87	10.0
88	4.5
89	3.0
90	4.0
91	3.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005098967923229939
3	0.0032572511509588267
4	0.0
5	4.566239931250692E-5
6	3.1963679518754844E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.4356362197677607E-4
26-27	0.002002075154704077
28-29	0.00784402543809834
30-31	0.0025695723141316643
32-33	0.005343487641636823
34-35	0.0029532595662789306
36-37	0.001141049526560819
38-39	3.8836248511600775E-5
40-41	3.914908370395119E-5
42-43	0.0026679451754631767
44-45	6.296507980584757E-4
46-47	3.546648952767261E-4
48-49	0.0017931020992335302
50-51	5.271021513262508E-4
52-53	0.0
54-55	0.005094581706725158
56-57	0.05018017123495877
58-59	0.07404298795336012
60-61	0.037034377793438766
62-63	0.01599504398637096
64-65	0.0017501476797593916
66-67	0.0
68-69	0.00232325015645779
70-71	0.0046756379134759935
72-73	0.016036330250629695
74-75	0.019555506794204643
76-77	0.02563240162820197
78-79	0.007332166001388415
80-81	0.008686637651032239
82-83	0.03627959263087971
84-85	0.021117667464146943
86-87	0.01589391875531704
88-89	0.008219539730219836
90-91	9.287355420384403E-5
92-93	0.0
94-95	0.0
96-97	5.3877406057199705E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	216.0
22-23	464.0
24-25	763.0
26-27	1926.0
28-29	5297.0
30-31	15862.0
32-33	20845.0
34-35	28764.0
36-37	45365.0
38-39	52209.0
40-41	50197.0
42-43	57801.0
44-45	69304.0
46-47	69628.0
48-49	65436.0
50-51	58705.0
52-53	55332.0
54-55	59849.0
56-57	61848.0
58-59	61877.0
60-61	59683.0
62-63	57453.0
64-65	59837.0
66-67	63899.0
68-69	65941.0
70-71	66178.0
72-73	59408.0
74-75	59801.0
76-77	63099.0
78-79	65009.0
80-81	64400.0
82-83	60027.0
84-85	58225.0
86-87	60626.0
88-89	63214.0
90-91	63370.0
92-93	61507.0
94-95	67961.0
96-97	186806.0
98-99	1393439.0
100-101	3088352.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.04897792930937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.35037902386922	80.43307506506707
2	6.398287948435409	11.267254287143109
3	1.3626403439624366	3.599372687134053
4	0.4354945384470771	1.5337939601624584
5	0.18414536528272063	0.810690560178144
6	0.09515541212463734	0.5027002069209918
7	0.05267734809856946	0.32467306620738423
8	0.030151493475002122	0.21238465468129422
9	0.022441397697211103	0.17783479174901354
>10	0.06655292584598747	0.8764833165426693
>50	0.0012035342861238744	0.07145598019561139
>100	7.706322616443381E-4	0.1356492858418311
>500	1.0003621397510418E-4	0.05463213817635537
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.522079977083564E-5	0.0	0.0	0.0
3	0.0	1.522079977083564E-5	0.0	0.0	0.0
4	0.0	3.044159954167128E-5	0.0	0.0	1.522079977083564E-5
5	0.0	3.044159954167128E-5	0.0	0.0	1.522079977083564E-5
6	6.088319908334256E-5	3.044159954167128E-5	0.0	0.0	1.522079977083564E-5
7	6.088319908334256E-5	3.044159954167128E-5	0.0	0.0	1.522079977083564E-5
8	6.088319908334256E-5	4.566239931250691E-5	0.0	0.0	1.522079977083564E-5
9	6.088319908334256E-5	1.0654559839584948E-4	0.0	0.0	1.522079977083564E-5
10-11	7.610399885417819E-5	1.0654559839584948E-4	0.0	7.61039988541782E-6	1.522079977083564E-5
12-13	7.610399885417819E-5	1.0654559839584948E-4	0.0	1.522079977083564E-5	1.522079977083564E-5
14-15	7.610399885417819E-5	1.5220799770835638E-4	0.0	1.522079977083564E-5	4.566239931250691E-5
16-17	1.5220799770835638E-4	1.8264959725002765E-4	0.0	3.044159954167128E-5	4.566239931250691E-5
18-19	1.6742879747919201E-4	2.1309119679169893E-4	0.0	6.849359896876037E-5	6.849359896876037E-5
20-21	1.6742879747919201E-4	2.283119965625346E-4	0.0	8.371439873959601E-5	8.371439873959601E-5
22-23	1.6742879747919201E-4	2.283119965625346E-4	0.0	9.132479862501383E-5	1.0654559839584946E-4
24-25	1.7503919736460983E-4	2.359223964479524E-4	0.0	1.141559982812673E-4	1.4459759782293856E-4
26-27	1.8264959725002765E-4	2.4353279633337023E-4	0.0	1.5220799770835638E-4	1.5220799770835638E-4
28-29	1.8264959725002765E-4	2.51143196218788E-4	0.0	4.033511939271444E-4	1.5220799770835638E-4
30-31	1.8264959725002765E-4	3.0441599541671275E-4	0.0	0.001347040779718954	1.5220799770835638E-4
32-33	1.8264959725002765E-4	3.4246799484380185E-4	0.0	0.003660602344885971	1.5220799770835638E-4
34-35	1.8264959725002765E-4	4.185719936979801E-4	0.0	0.0066438790999697565	1.5220799770835638E-4
36-37	1.8264959725002765E-4	4.566239931250692E-4	0.0	0.011095963032939181	1.5220799770835638E-4
38-39	1.8264959725002765E-4	4.7945519278132264E-4	0.0	0.017092958142648423	1.5220799770835638E-4
40-41	1.978703970208633E-4	5.327279919792473E-4	0.0	0.02391187643998279	1.5220799770835638E-4
42-43	1.978703970208633E-4	5.47948791750083E-4	0.0	0.03000019634831704	1.598183975937742E-4
44-45	1.978703970208633E-4	6.088319908334255E-4	0.0	0.03723768663934939	1.8264959725002765E-4
46-47	2.054807969062811E-4	6.697151899167681E-4	0.0	0.04498507372270473	1.8264959725002765E-4
48-49	2.1309119679169895E-4	7.001567894584393E-4	0.0	0.052230174413622495	2.1309119679169895E-4
50-51	2.1309119679169895E-4	7.838711881980353E-4	0.0	0.0600460550959466	2.1309119679169895E-4
52-53	2.2070159667711677E-4	7.914815880834532E-4	0.0	0.06704762299053099	2.2070159667711677E-4
54-55	2.283119965625346E-4	7.914815880834532E-4	0.0	0.07385132048809452	2.283119965625346E-4
56-57	2.283119965625346E-4	8.143127877397067E-4	0.0	0.08086810918244974	2.51143196218788E-4
58-59	2.283119965625346E-4	8.295335875105423E-4	0.0	0.08797622267542998	2.739743958750415E-4
60-61	2.359223964479524E-4	8.675855869376315E-4	0.0	0.09516805056714983	2.739743958750415E-4
62-63	2.5875359610420584E-4	8.980271864793027E-4	0.0	0.10219244966139049	3.0441599541671275E-4
64-65	2.6636399598962366E-4	9.741311853334809E-4	0.0	0.10833404236892266	3.0441599541671275E-4
66-67	2.739743958750415E-4	9.969623849897342E-4	0.0	0.11470394707301737	3.0441599541671275E-4
68-69	2.739743958750415E-4	0.0010197935846459876	0.0	0.121035799777685	3.1202639530213057E-4
70-71	2.739743958750415E-4	0.0010350143844168234	0.0	0.12681209329071713	3.3485759495838403E-4
72-73	2.891951956458771E-4	0.001050235184187659	0.0	0.13148487882036367	3.3485759495838403E-4
74-75	3.1202639530213057E-4	0.0010882871836147483	0.0	0.1350769875662809	3.3485759495838403E-4
76-77	3.196367951875484E-4	0.0011111183832710017	0.0	0.1382961867178126	3.4246799484380185E-4
78-79	3.3485759495838403E-4	0.0011263391830418372	0.0	0.14060974828297962	3.5007839472921967E-4
80-81	3.3485759495838403E-4	0.0011491703826980908	0.0	0.14142406107071934	3.5007839472921967E-4
82-83	3.3485759495838403E-4	0.0011567807825835085	0.0	0.14207855546086529	3.652991945000553E-4
84-85	3.3485759495838403E-4	0.0011720015823543442	0.0	0.1423525298567403	3.652991945000553E-4
86-87	3.3485759495838403E-4	0.0011872223821251797	0.0	0.14249712745456325	3.652991945000553E-4
88	3.3485759495838403E-4	0.0012328847814376868	0.0	0.14249712745456325	3.652991945000553E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11665	0.0	52.769814	1
GTATCAA	21885	0.0	36.962036	1
ATCAACG	26605	0.0	29.90116	3
TCAACGC	26710	0.0	29.829836	4
TATCAAC	27000	0.0	29.588573	2
CAACGCA	27300	0.0	29.15457	5
TGGTATC	4900	0.0	28.465788	2
AACGCAG	28295	0.0	28.129341	6
GTGGTAT	5175	0.0	27.4373	1
ACGCAGA	32265	0.0	24.668207	7
CGCAGAG	32550	0.0	24.43939	8
GCAGAGT	36900	0.0	21.614908	9
GAGTACT	19875	0.0	20.327793	12-13
CAGAGTA	36825	0.0	19.079136	10-11
TACATGG	28410	0.0	18.697721	2
GTACATG	28935	0.0	18.618258	1
GTACTTT	21765	0.0	18.44783	14-15
ACATGGG	28510	0.0	18.13342	3
AGAGTAC	34755	0.0	17.716333	10-11
AGTACTT	21545	0.0	16.697643	12-13
>>END_MODULE