FastQCFastQC Report
Fri 27 May 2016
SRR2139600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6810063
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108550.051.314741
GTATCAA193500.035.164391
ATCAACG233650.028.8510533
TCAACGC236750.028.4895424
CAACGCA239700.028.173945
TATCAAC242950.027.9552042
AACGCAG247100.027.3471936
GTGGTAT48050.025.334111
TGGTATC46600.024.861322
ACGCAGA286100.023.6338277
CGCAGAG290950.023.254298
GCAGAGT334550.020.3240559
GAGTACT180750.019.9931512-13
GTACATG263050.019.7393321
TACATGG260250.019.3065412
CAGAGTA329100.018.61369710-11
ACATGGG263350.018.488713
GTACTTT200800.017.88218914-15
AGAGTAC310700.017.04804210-11
CATGGGG166350.016.8039364
Produced by FastQC (version 0.11.5)