##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139600_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6810063
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.206924517438384	32.0	25.0	33.0	18.0	33.0
2	29.70221978856877	31.0	28.0	33.0	25.0	33.0
3	32.03285549634416	33.0	31.0	33.0	29.0	34.0
4	31.92337134032387	33.0	31.0	33.0	29.0	34.0
5	32.87246373491699	33.0	33.0	34.0	32.0	34.0
6	36.853421620328625	38.0	37.0	38.0	35.0	38.0
7	37.02174135540302	38.0	38.0	38.0	35.0	38.0
8	37.14676721786568	38.0	38.0	38.0	36.0	38.0
9	37.2248899900045	38.0	38.0	38.0	36.0	38.0
10-11	37.336427063890596	38.0	38.0	38.0	36.5	38.0
12-13	37.3938594253827	38.0	38.0	38.0	37.0	38.0
14-15	37.40055958366318	38.0	38.0	38.0	37.0	38.0
16-17	37.45335197339584	38.0	38.0	38.0	37.0	38.0
18-19	37.44169341752051	38.0	38.0	38.0	37.0	38.0
20-21	37.45587793962725	38.0	38.0	38.0	37.0	38.0
22-23	37.46271320209334	38.0	38.0	38.0	37.0	38.0
24-25	37.476248517665454	38.0	38.0	38.0	37.0	38.0
26-27	37.43775029101734	38.0	38.0	38.0	37.0	38.0
28-29	37.40714926605338	38.0	38.0	38.0	37.0	38.0
30-31	37.42252851497179	38.0	38.0	38.0	37.0	38.0
32-33	37.41306764651551	38.0	38.0	38.0	37.0	38.0
34-35	37.38265657665012	38.0	38.0	38.0	37.0	38.0
36-37	37.360466968981285	38.0	38.0	38.0	37.0	38.0
38-39	37.363446182956764	38.0	38.0	38.0	37.0	38.0
40-41	37.36565031366249	38.0	38.0	38.0	37.0	38.0
42-43	37.3311157886953	38.0	38.0	38.0	37.0	38.0
44-45	37.29462643515407	38.0	38.0	38.0	37.0	38.0
46-47	37.26927922505892	38.0	38.0	38.0	37.0	38.0
48-49	37.21439253025713	38.0	38.0	38.0	37.0	38.0
50-51	37.17274899382167	38.0	38.0	38.0	37.0	38.0
52-53	37.162592412353746	38.0	38.0	38.0	37.0	38.0
54-55	37.14959657476294	38.0	38.0	38.0	37.0	38.0
56-57	37.10524349934765	38.0	38.0	38.0	36.0	38.0
58-59	37.08902798233031	38.0	38.0	38.0	36.0	38.0
60-61	37.11462770549531	38.0	38.0	38.0	36.0	38.0
62-63	37.126990189619605	38.0	38.0	38.0	36.0	38.0
64-65	37.13598975250231	38.0	38.0	38.0	36.0	38.0
66-67	37.13830055808542	38.0	38.0	38.0	36.0	38.0
68-69	37.13232875898849	38.0	38.0	38.0	36.0	38.0
70-71	37.12833244955824	38.0	38.0	38.0	36.0	38.0
72-73	37.1285766105799	38.0	38.0	38.0	36.0	38.0
74-75	37.116454936927234	38.0	38.0	38.0	36.0	38.0
76-77	37.102573720814064	38.0	38.0	38.0	36.0	38.0
78-79	37.10935661982802	38.0	38.0	38.0	36.0	38.0
80-81	37.1114288421212	38.0	38.0	38.0	36.0	38.0
82-83	37.071196823723014	38.0	38.0	38.0	36.0	38.0
84-85	37.060295402800506	38.0	38.0	38.0	36.0	38.0
86-87	37.06042654740645	38.0	38.0	38.0	36.0	38.0
88-89	37.052781313337675	38.0	38.0	38.0	36.0	38.0
90-91	37.032060540239065	38.0	38.0	38.0	36.0	38.0
92-93	37.02661955060785	38.0	38.0	38.0	36.0	38.0
94-95	37.0120511384315	38.0	38.0	38.0	36.0	38.0
96-97	36.99196228200023	38.0	38.0	38.0	36.0	38.0
98-99	36.95955796051814	38.0	38.0	38.0	35.5	38.0
100	35.65440979206512	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	6.0
20	29.0
21	74.0
22	324.0
23	1108.0
24	2663.0
25	5396.0
26	9727.0
27	16230.0
28	26290.0
29	38844.0
30	52203.0
31	67149.0
32	86626.0
33	115645.0
34	173471.0
35	320940.0
36	986832.0
37	4906504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17841494271052	21.364192959742077	14.188326892130073	23.269065205417334
2	15.021608156721216	20.811689565629717	42.219631798595444	21.94707047905362
3	18.326185816256043	26.671937483259477	30.34972710014238	24.652149600342092
4	12.787106374786841	18.04040579360279	38.886600608540626	30.28588722306974
5	13.328076404613176	37.82438040193479	35.622417423635035	13.225125769817007
6	29.939741928170193	38.06163016322073	18.61653716673113	13.38209074187795
7	26.557302039643393	33.172116028882556	23.024794337438582	17.245787594035473
8	24.161156805744675	37.488581236326304	20.959717993798296	17.39054396413073
9	25.711538938773398	17.34290857514828	21.59264899605187	35.35290349002645
10-11	24.175987799231812	27.244762640228142	28.741863327842932	19.837386232697114
12-13	25.06600893413174	24.88413102786274	29.037675569227478	21.01218446877804
14-15	22.2124229981426	26.211982179900538	26.957959126075632	24.617635695881226
16-17	21.416505251126164	29.348634513366466	27.695624842237144	21.53923539327022
18-19	21.390932800474825	28.372285249049828	29.692838377559795	20.543943572915552
20-21	22.379544183511918	27.38280007850272	29.602851296164857	20.63480444182051
22-23	22.04695807939511	27.31001240461392	29.489865942939364	21.153163573051604
24-25	21.736159260897093	27.476981240590444	29.60958615506786	21.177273343444604
26-27	21.635694306818344	27.615036241729822	29.551169832450487	21.198099619001344
28-29	21.515450678024063	27.62533823317409	29.63160670621985	21.227604382582005
30-31	21.85352597918611	27.489968537963094	29.517144423114704	21.13936105973609
32-33	21.62428773216635	27.627018086050537	29.477668288991016	21.271025892792096
34-35	21.754714486955034	27.722042132730063	29.484751720244862	21.038491660070044
36-37	21.725707915273134	27.59817911557042	29.41288740245262	21.263225566703827
38-39	21.581056023008912	27.690837789469224	29.547784339935852	21.180321847586008
40-41	21.794326245948035	27.611235119805066	29.414485165596666	21.179953468650233
42-43	21.74260884894158	27.70292089992379	29.14802209917884	21.40644815195579
44-45	21.819508105695217	27.835605561424376	29.018694032259624	21.326192300620782
46-47	21.873521516799133	27.817807408493145	28.761340670651407	21.547330404056314
48-49	21.878216775326536	27.92524365795047	28.6888123396431	21.507727227079886
50-51	21.821379727032507	28.041140727352186	28.596203871682597	21.54127567393271
52-53	21.96188497708274	28.030024701597128	28.47125623271736	21.536834088602774
54-55	21.966182165669164	28.024688427299704	28.42438666197254	21.584742745058595
56-57	21.978899255154015	28.12067152066069	28.344575693381845	21.555853530803446
58-59	21.868895148842554	28.138538272344782	28.49866904798984	21.493897530822824
60-61	21.992477882454825	28.1914531434312	28.40353675041835	21.412532223695624
62-63	21.940787275838584	28.294955739156833	28.371943131644507	21.392313853360076
64-65	21.88256353624039	28.234902682923035	28.434052557510768	21.448481223325803
66-67	22.027151618602936	28.365726689058125	28.160788667584256	21.446333024754683
68-69	21.999869238561633	28.223992789292623	28.328826497276765	21.447311474868975
70-71	21.96659978235294	27.904633715548133	28.49255163875306	21.636214863345867
72-73	22.110173090664357	28.102635622032924	28.28046813321638	21.506723154086338
74-75	21.88679140809853	28.144216227531743	28.317437454687504	21.651554909682226
76-77	21.938209957921732	27.965434417495892	28.38104310765174	21.715312516930634
78-79	21.954552034636936	27.969071162174096	28.332930709228833	21.743446093960138
80-81	21.963396726676912	28.03774945958242	28.197781094323183	21.801072719417487
82-83	22.01781240000524	27.914420390604093	28.372174183153913	21.695593026236754
84-85	22.104382877541376	27.884969862720826	28.252614869553334	21.758032390184464
86-87	21.929099816178898	28.002161002947222	28.314323944176707	21.754415236697174
88-89	22.01008846745885	27.95425330055248	28.255017684876098	21.780640547112572
90-91	22.026319449706154	27.986908202251826	28.24411962502278	21.74265272301924
92-93	22.072725659432294	28.00862618359165	28.189631925216453	21.729016231759605
94-95	21.952088076838066	28.05397777956688	28.246758435485468	21.747175708109584
96-97	22.06142596157463	28.169737119716594	28.275566755367432	21.49327016334134
98-99	23.108135434821577	30.092431481504594	28.443733400010313	18.355699683663516
100	29.233710696178193	40.973643119505375	29.792646184316425	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1846.0
1	1620.5
2	1663.0
3	2082.5
4	2745.0
5	3949.5
6	5020.5
7	6326.5
8	7875.5
9	8866.0
10	9068.0
11	9178.5
12	10353.0
13	13140.0
14	18502.5
15	24710.0
16	29336.5
17	31718.5
18	31645.5
19	29715.0
20	27348.0
21	25754.5
22	26050.5
23	29478.0
24	36260.0
25	46131.5
26	59341.0
27	74542.0
28	89912.0
29	107241.0
30	124206.5
31	139517.5
32	157930.5
33	178059.5
34	195582.5
35	213722.0
36	233784.0
37	248515.0
38	258632.5
39	266723.5
40	274433.5
41	281057.0
42	285126.5
43	289834.0
44	294322.5
45	297943.5
46	296256.0
47	292481.5
48	288895.0
49	282124.5
50	273227.5
51	261410.0
52	247761.0
53	231689.0
54	213506.0
55	195425.5
56	176456.0
57	156835.5
58	137892.0
59	118579.0
60	99337.5
61	79789.5
62	62151.0
63	48318.5
64	36979.0
65	27880.5
66	20271.5
67	14892.0
68	11126.5
69	8164.0
70	6019.0
71	4389.5
72	3139.0
73	2169.5
74	1486.5
75	1000.5
76	648.0
77	391.5
78	260.0
79	179.5
80	120.5
81	82.0
82	62.0
83	46.5
84	29.0
85	18.5
86	14.0
87	12.5
88	7.5
89	2.5
90	2.0
91	2.0
92	1.5
93	1.5
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005374399620091621
3	0.003509512320223763
4	0.0
5	4.4052455902390334E-5
6	3.083671913167323E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.717023540365387E-4
26-27	0.0017993482833960326
28-29	0.00717819128829137
30-31	0.0025818638408865724
32-33	0.005264583670972074
34-35	0.0026851809523471366
36-37	0.0010925187216460734
38-39	1.496728600298328E-5
40-41	3.770405813302173E-5
42-43	0.002363045733444946
44-45	5.520359007347444E-4
46-47	4.1834096663568107E-4
48-49	0.002097672155288478
50-51	4.662846925690184E-4
52-53	0.0
54-55	0.005294695254480515
56-57	0.04961442780410085
58-59	0.0734178293618624
60-61	0.03653648392179153
62-63	0.014851044194162584
64-65	0.001639901612665721
66-67	0.0
68-69	0.002625524014433127
70-71	0.004080169832919229
72-73	0.015232386669364436
74-75	0.018626117567054026
76-77	0.024013158488651525
78-79	0.0066302998676405845
80-81	0.008974572383808171
82-83	0.03541016209922964
84-85	0.02079827152081993
86-87	0.015677919854741536
88-89	0.0069307790379555985
90-91	8.821694153432002E-5
92-93	0.0
94-95	0.0
96-97	5.111058705722515E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	58.0
20-21	304.0
22-23	630.0
24-25	770.0
26-27	1913.0
28-29	5330.0
30-31	15430.0
32-33	20031.0
34-35	27969.0
36-37	43413.0
38-39	51554.0
40-41	49076.0
42-43	56019.0
44-45	66275.0
46-47	67172.0
48-49	62758.0
50-51	58197.0
52-53	55002.0
54-55	59372.0
56-57	61292.0
58-59	61472.0
60-61	59238.0
62-63	57057.0
64-65	60321.0
66-67	63691.0
68-69	65899.0
70-71	65690.0
72-73	59649.0
74-75	60274.0
76-77	63114.0
78-79	64866.0
80-81	64895.0
82-83	60153.0
84-85	58955.0
86-87	61111.0
88-89	63678.0
90-91	64275.0
92-93	62275.0
94-95	69700.0
96-97	198321.0
98-99	1487428.0
100-101	3235436.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.77146205955077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.63444302848497	78.644831345347
2	6.742091628314719	11.70042295856651
3	1.5415137389489615	4.012782027404582
4	0.5320919479101347	1.8468158508110688
5	0.21689268960073577	0.9410047893342084
6	0.11424951233291011	0.5948158334830372
7	0.0675821257331815	0.4104939901272384
8	0.040785961705095415	0.2831246022924782
9	0.02735141525557372	0.21359900620116298
>10	0.08055178227131576	1.086407989617177
>50	0.0017339550134779374	0.09941277044186254
>100	6.354217345036852E-4	0.11600226948479103
>500	7.679269446507095E-5	0.050286566888848284
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.4684151967463443E-5	0.0	0.0	0.0
2	0.0	4.405245590239033E-5	0.0	0.0	0.0
3	0.0	4.405245590239033E-5	0.0	2.9368303934926886E-5	0.0
4	0.0	7.342075983731722E-5	0.0	2.9368303934926886E-5	0.0
5	0.0	8.810491180478065E-5	0.0	2.9368303934926886E-5	0.0
6	0.0	8.810491180478065E-5	0.0	2.9368303934926886E-5	0.0
7	0.0	1.027890637722441E-4	0.0	2.9368303934926886E-5	0.0
8	0.0	1.32157367707171E-4	0.0	5.873660786985377E-5	0.0
9	0.0	2.349464314794151E-4	0.0	8.810491180478065E-5	0.0
10-11	0.0	2.569726594306102E-4	0.0	1.1747321573970754E-4	0.0
12-13	0.0	2.64314735414342E-4	0.0	1.32157367707171E-4	0.0
14-15	0.0	3.3039341926792746E-4	0.0	1.32157367707171E-4	0.0
16-17	0.0	3.377354952516592E-4	0.0	1.4684151967463445E-4	0.0
18-19	0.0	3.597617232028544E-4	0.0	1.4684151967463445E-4	0.0
20-21	0.0	3.8178795115404953E-4	0.0	1.6886774762582958E-4	0.0
22-23	0.0	4.331824830401716E-4	0.0	2.2026227951195166E-4	0.0
24-25	0.0	4.8457701492629363E-4	0.0	3.0836719131673235E-4	0.0
26-27	0.0	5.139453188612205E-4	0.0	3.671037991865861E-4	0.0
28-29	0.0	5.28629470828684E-4	0.0	5.873660786985377E-4	0.0
30-31	0.0	6.461026865683915E-4	0.0	0.0013215736770717098	0.0
32-33	0.0	6.681289145195866E-4	0.0	0.003347986648581665	0.0
34-35	0.0	8.002862822267577E-4	0.0	0.005983791926741353	0.0
36-37	0.0	9.544698778851239E-4	0.0	0.010117380705582314	0.0
38-39	0.0	9.838381818200507E-4	0.0	0.016482960583477715	0.0
40-41	0.0	0.001057258941657368	0.0	0.022995381981047752	0.0
42-43	1.4684151967463443E-5	0.0010646010176410996	0.0	0.029052594667626424	0.0
44-45	1.4684151967463443E-5	0.0011380217774784168	0.0	0.03612301383996007	0.0
46-47	1.4684151967463443E-5	0.0012334687652669292	0.0	0.04312735432844013	0.0
48-49	1.4684151967463443E-5	0.0012481529172343926	0.0	0.05031524671651349	0.0
50-51	1.4684151967463443E-5	0.0013876523609252954	0.0	0.057238824369172506	0.0
52-53	1.4684151967463443E-5	0.001394994436909027	0.0	0.06437532222535973	0.0
54-55	1.4684151967463443E-5	0.0014096785888764907	0.0	0.07132826818195367	0.0
56-57	1.4684151967463443E-5	0.0014390468928114175	0.0	0.07773055843976773	0.0
58-59	1.4684151967463443E-5	0.0014390468928114175	0.0	0.08519010763923916	0.0
60-61	1.4684151967463443E-5	0.0014390468928114175	0.0	0.09287726119420628	0.0
62-63	2.2026227951195164E-5	0.001453731044778881	0.0	0.09974944431497917	0.0
64-65	2.9368303934926886E-5	0.0015124676526487347	0.0	0.1060269192810698	0.0
66-67	2.9368303934926886E-5	0.0015418359565836615	0.0	0.11244389369085131	0.0
68-69	2.9368303934926886E-5	0.001556520108551125	0.0	0.11879478941677926	0.0
70-71	2.9368303934926886E-5	0.0015712042605185883	0.0	0.12482997587540673	0.0
72-73	2.9368303934926886E-5	0.001585888412486052	0.0	0.12925724769359698	0.0
74-75	2.9368303934926886E-5	0.0016225987924047105	0.0	0.13248776112643892	0.0
76-77	2.9368303934926886E-5	0.0017033616282257596	0.0	0.13629829856199568	0.0
78-79	2.9368303934926886E-5	0.0017474140841281498	0.0	0.13877257816851327	0.0
80-81	2.9368303934926886E-5	0.0017547561601118815	0.0	0.13960223275467495	0.0
82-83	2.9368303934926886E-5	0.001769440312079345	0.0	0.14019694090935722	0.0
84-85	2.9368303934926886E-5	0.0017767823880630767	0.0	0.14043922941682035	0.0
86-87	2.9368303934926886E-5	0.0018061506919980035	0.0	0.14056404470854383	0.0
88	2.9368303934926886E-5	0.0018648872998678573	0.0	0.14057138678452755	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10855	0.0	51.31474	1
GTATCAA	19350	0.0	35.16439	1
ATCAACG	23365	0.0	28.851053	3
TCAACGC	23675	0.0	28.489542	4
CAACGCA	23970	0.0	28.17394	5
TATCAAC	24295	0.0	27.955204	2
AACGCAG	24710	0.0	27.347193	6
GTGGTAT	4805	0.0	25.33411	1
TGGTATC	4660	0.0	24.86132	2
ACGCAGA	28610	0.0	23.633827	7
CGCAGAG	29095	0.0	23.25429	8
GCAGAGT	33455	0.0	20.324055	9
GAGTACT	18075	0.0	19.99315	12-13
GTACATG	26305	0.0	19.739332	1
TACATGG	26025	0.0	19.306541	2
CAGAGTA	32910	0.0	18.613697	10-11
ACATGGG	26335	0.0	18.48871	3
GTACTTT	20080	0.0	17.882189	14-15
AGAGTAC	31070	0.0	17.048042	10-11
CATGGGG	16635	0.0	16.803936	4
>>END_MODULE