FastQCFastQC Report
Fri 27 May 2016
SRR2139599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6951952
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124600.053.562211
GTATCAA230800.037.2947651
ATCAACG285650.029.5892983
TCAACGC286150.029.580514
TATCAAC291550.029.3078772
CAACGCA291750.028.9695935
AACGCAG303350.027.9032886
TGGTATC50100.026.7928312
GTGGTAT55000.025.854931
ACGCAGA345800.024.4779157
CGCAGAG353050.023.9752528
GCAGAGT399200.021.2455949
GAGTACT225650.020.23351512-13
GTACATG293150.019.8898371
TACATGG293050.019.5387732
CAGAGTA394850.019.12135310-11
ACATGGG293800.019.0314643
GTACTTT249700.018.1757214-15
AGAGTAC373350.017.93060710-11
CATGGGG178150.017.1876014
Produced by FastQC (version 0.11.5)