FastQCFastQC Report
Fri 27 May 2016
SRR2139598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7881754
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115650.051.6642381
GTATCAA203850.033.7509881
TCAACGC250250.027.831814
ATCAACG251850.027.6384723
CAACGCA256450.027.175515
TATCAAC260150.026.8551982
AACGCAG267900.026.061626
GTGGTAT54800.025.0479581
TGGTATC53550.024.600992
ACGCAGA314950.022.1548167
CGCAGAG320500.021.7975468
GTACATG314150.019.6146811
TACATGG325550.019.6066132
TATACAC79550.019.498213
ACATGGG325650.019.0260473
GCAGAGT373600.018.6994489
CTTATAC55300.018.6737481
GAGTACT180450.018.65100112-13
CAGAGTA368500.017.74164210-11
CATGGGG218800.017.3048154
Produced by FastQC (version 0.11.5)