##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139598_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7881754
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.66005523643595	18.0	18.0	30.0	18.0	32.0
2	25.716372015670622	27.0	25.0	29.0	18.0	31.0
3	29.00422367914553	29.0	27.0	31.0	25.0	33.0
4	27.37394024223542	29.0	27.0	31.0	15.0	33.0
5	30.958353813123324	32.0	31.0	33.0	29.0	33.0
6	35.65267959390765	37.0	35.0	38.0	31.0	38.0
7	36.17838656217893	38.0	36.0	38.0	33.0	38.0
8	36.444628061215816	38.0	37.0	38.0	34.0	38.0
9	36.77075369264253	38.0	37.0	38.0	34.0	38.0
10-11	37.03550136175272	38.0	38.0	38.0	35.5	38.0
12-13	37.16546190099311	38.0	38.0	38.0	36.0	38.0
14-15	37.21899199086904	38.0	38.0	38.0	36.0	38.0
16-17	37.38158714418136	38.0	38.0	38.0	36.5	38.0
18-19	37.4058995624578	38.0	38.0	38.0	37.0	38.0
20-21	37.454787277916054	38.0	38.0	38.0	37.0	38.0
22-23	37.474458064565	38.0	38.0	38.0	37.0	38.0
24-25	37.50081610737441	38.0	38.0	38.0	37.0	38.0
26-27	37.45790629203276	38.0	38.0	38.0	37.0	38.0
28-29	37.42574309554973	38.0	38.0	38.0	37.0	38.0
30-31	37.4498798463178	38.0	38.0	38.0	37.0	38.0
32-33	37.44434027030758	38.0	38.0	38.0	37.0	38.0
34-35	37.40832784676009	38.0	38.0	38.0	37.0	38.0
36-37	37.39055226029386	38.0	38.0	38.0	37.0	38.0
38-39	37.3944164494076	38.0	38.0	38.0	37.0	38.0
40-41	37.409236267109364	38.0	38.0	38.0	37.0	38.0
42-43	37.387076630962376	38.0	38.0	38.0	37.0	38.0
44-45	37.35820696056541	38.0	38.0	38.0	37.0	38.0
46-47	37.33481851245868	38.0	38.0	38.0	37.0	38.0
48-49	37.278626464545795	38.0	38.0	38.0	37.0	38.0
50-51	37.2036450033297	38.0	38.0	38.0	37.0	38.0
52-53	37.234272877046166	38.0	38.0	38.0	37.0	38.0
54-55	37.22806732171041	38.0	38.0	38.0	37.0	38.0
56-57	37.19034042336372	38.0	38.0	38.0	37.0	38.0
58-59	37.1856407878538	38.0	38.0	38.0	37.0	38.0
60-61	37.20877255764281	38.0	38.0	38.0	37.0	38.0
62-63	37.21568722942344	38.0	38.0	38.0	37.0	38.0
64-65	37.16372670431798	38.0	38.0	38.0	36.0	38.0
66-67	37.2107493384856	38.0	38.0	38.0	37.0	38.0
68-69	37.22775518005226	38.0	38.0	38.0	37.0	38.0
70-71	37.21679434994704	38.0	38.0	38.0	37.0	38.0
72-73	37.21384787938936	38.0	38.0	38.0	37.0	38.0
74-75	37.18242260205932	38.0	38.0	38.0	36.0	38.0
76-77	37.19418564518942	38.0	38.0	38.0	36.0	38.0
78-79	37.20124981900184	38.0	38.0	38.0	36.0	38.0
80-81	37.200787273520056	38.0	38.0	38.0	36.0	38.0
82-83	37.161913437191075	38.0	38.0	38.0	36.0	38.0
84-85	37.15614350630451	38.0	38.0	38.0	36.0	38.0
86-87	37.1306006771524	38.0	38.0	38.0	36.0	38.0
88-89	37.14509654181011	38.0	38.0	38.0	36.0	38.0
90-91	37.1392631182881	38.0	38.0	38.0	36.0	38.0
92-93	37.12383911001878	38.0	38.0	38.0	36.0	38.0
94-95	37.11694157778379	38.0	38.0	38.0	36.0	38.0
96-97	37.10415912629746	38.0	38.0	38.0	36.0	38.0
98-99	37.13182242655696	38.0	38.0	38.0	36.0	38.0
100	35.8394300857752	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	12.0
20	21.0
21	76.0
22	361.0
23	1039.0
24	2601.0
25	5486.0
26	10210.0
27	17293.0
28	28136.0
29	41782.0
30	57091.0
31	76394.0
32	103267.0
33	149509.0
34	254465.0
35	558184.0
36	1924736.0
37	4651090.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.547136335389304	25.93898515482721	14.184647224462982	20.3292312853205
2	15.54583673523764	21.565618214033545	40.31517652156316	22.57336852916566
3	18.25756459821198	26.970439427832716	30.238714304727633	24.53328166922767
4	12.92204306429956	18.410494929488365	38.14528983351284	30.52217217269923
5	13.41112650813512	37.92778866226985	35.42230574565002	13.23877908394502
6	29.817525388384365	37.864617444289685	18.59110294485212	13.726754222473831
7	26.4652743838537	33.058216138280265	23.02505337659286	17.451456101273173
8	24.308612017071326	37.22406205522273	21.066275349370205	17.401050578335735
9	25.583759655528453	17.284946980075755	21.57167046827394	35.55962289612185
10-11	24.19727893055277	27.146609752093255	28.621611382441014	20.034499934912965
12-13	24.97560187745012	24.77706738880711	28.957260021056225	21.290070712686543
14-15	22.204518182120374	26.195406504694258	26.490677075178954	25.109398238006413
16-17	21.516555832623045	29.42772636649152	27.362240689064897	21.693477111820542
18-19	21.57758285782581	28.41241302380155	29.164092155121818	20.845911963250817
20-21	22.535145148788878	27.360371931282085	29.088844231128146	21.015638688800887
22-23	22.37062611607964	27.344296977357523	28.918227793044416	21.366849113518416
24-25	21.979865761167098	27.5541849092245	29.017732435548567	21.448216894059836
26-27	21.853526844791013	27.630305266828657	29.068196088722758	21.447971799657573
28-29	21.661243005514596	27.422222896446417	29.25876941234104	21.65776468569794
30-31	21.949719475656526	27.383885108085703	29.168387925019506	21.498007491238262
32-33	21.703214997988315	27.745811036008316	29.006427143515047	21.54454682248832
34-35	21.83862173800688	27.655350725181087	29.17265112326651	21.333376413545516
36-37	21.939057713528463	27.457496846031926	28.97394688049043	21.62949855994918
38-39	21.759881892420086	27.55296760904265	29.147204497871854	21.539946000665413
40-41	21.859073807700018	27.381747864917482	29.157433185677718	21.601745141704782
42-43	21.887147242397656	27.475853482421105	28.879370327348912	21.75762894783233
44-45	21.82262397994851	27.58350730173153	28.81190745634473	21.781961261975226
46-47	22.04482927110464	27.523560523066447	28.521459036707164	21.910151169121747
48-49	21.949415335217605	27.657205189487854	28.455222531704987	21.938156943589554
50-51	21.91550204999151	27.780216820689517	28.39924049827752	21.90504063104145
52-53	22.05130527427953	27.711781329849494	28.434804349691213	21.802109046179766
54-55	22.00115881833529	27.766135665342944	28.261259636080442	21.971445880241323
56-57	22.028061879330032	27.9471078531358	28.13252660980473	21.892303657729435
58-59	21.876359993090052	28.000968849011436	28.30430485888703	21.818366299011483
60-61	22.02403908207067	28.20946443572403	28.063671769610142	21.70282471259516
62-63	21.933512594359588	28.289665458547436	28.107510135918584	21.669311811174392
64-65	21.953863178993394	28.180387477477424	28.062739216930876	21.803010126598306
66-67	22.04591771154186	28.140047923017413	27.995982412774318	21.818051952666412
68-69	21.94070021463925	28.038673262061153	28.17240041899462	21.84822610430498
70-71	22.052908617527052	27.783862099837176	28.25505679466821	21.908172487967565
72-73	22.098263580807597	27.888763828541265	28.04112228608446	21.971850304566683
74-75	21.934129544162865	27.98193936011518	28.09371095891855	21.9902201368034
76-77	22.112206062665003	27.759600095588944	28.114174079291775	22.01401976245428
78-79	22.01137866164926	27.68856960449225	28.119419273889513	22.180632459968972
80-81	22.08936039015245	27.886762676905924	27.936935153600178	22.086941779341448
82-83	22.149064440656765	27.735686728312626	28.07719523005566	22.03805360097495
84-85	22.070366957340298	27.826034197850774	28.026812915834252	22.07678592897468
86-87	22.062946967428648	27.85585963579493	28.07807356775817	22.003119829018253
88-89	22.137582599204343	27.749708473396435	28.053846265610733	22.05886266178849
90-91	22.21057871872477	27.76499221473141	28.0157307007072	22.008698365836622
92-93	22.18376861005585	27.798440424147312	27.975448216682192	22.04234274911465
94-95	22.05752350873417	27.77380424321681	28.057364529807998	22.111307718241026
96-97	22.27406899571241	27.80500555783109	28.034999275289373	21.885926171167124
98-99	23.237059382814877	29.77517943342967	28.286352918622182	18.70140826513327
100	22.166678978088335	30.551302863506745	22.286369385139015	24.99564877326591
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1960.0
1	1719.5
2	1691.0
3	2035.0
4	2560.5
5	3511.5
6	4416.5
7	5925.0
8	7679.5
9	8610.0
10	8811.0
11	9059.0
12	10233.5
13	12859.0
14	18145.0
15	24202.5
16	28381.0
17	29986.5
18	29332.0
19	27573.0
20	25361.5
21	23533.5
22	23876.0
23	27613.5
24	34642.5
25	44824.5
26	57747.5
27	72886.0
28	90570.5
29	111110.0
30	132929.0
31	154579.5
32	177596.0
33	201455.0
34	223597.5
35	244194.0
36	267208.0
37	287027.5
38	301398.0
39	313326.5
40	320441.0
41	323787.0
42	325401.0
43	334746.0
44	345840.0
45	349613.0
46	345396.0
47	335535.0
48	326156.0
49	317412.5
50	308807.5
51	298827.5
52	286785.5
53	270628.0
54	251326.5
55	231172.5
56	209186.0
57	185031.0
58	162557.5
59	141238.0
60	118200.0
61	96066.0
62	76279.5
63	59299.5
64	45866.0
65	34601.0
66	25219.5
67	18958.0
68	14621.0
69	10846.5
70	7759.5
71	5549.0
72	3904.5
73	2585.0
74	1689.0
75	1116.5
76	719.0
77	458.5
78	310.5
79	189.0
80	111.5
81	77.5
82	60.5
83	44.0
84	29.5
85	18.5
86	14.5
87	12.5
88	11.0
89	11.5
90	6.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0017635668405788863
3	9.26189779584595E-4
4	6.343765613593116E-5
5	0.0
6	0.0
7	2.791256869980971E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.16874210209215E-4
24-25	2.9818637968363564E-4
26-27	6.9795240434134E-5
28-29	0.002767345751733923
30-31	0.00192506148601913
32-33	0.0043612744802793355
34-35	0.008983460472390832
36-37	0.0014105060517441582
38-39	0.0
40-41	1.8180690742879255E-4
42-43	6.601696387590955E-4
44-45	9.882374705653469E-5
46-47	4.654158151219449E-5
48-49	0.00417307947770757
50-51	0.0032953060429213948
52-53	3.8873887584491885E-4
54-55	0.0028735888994223264
56-57	0.043688835630117835
58-59	0.06736647938594129
60-61	0.03269851831925661
62-63	0.011868057987972845
64-65	9.567147021369481E-4
66-67	1.8165285790081669E-4
68-69	0.0013736135593140659
70-71	0.011289985582242753
72-73	0.017769366243461347
74-75	0.011967594665911095
76-77	0.014072793651017393
78-79	0.003143263444496471
80-81	0.01489298028022281
82-83	0.047960071401416546
84-85	0.026092744562739544
86-87	0.0038435177941954484
88-89	1.632513664547501E-4
90-91	1.6511701595245027E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	44.0
20-21	188.0
22-23	447.0
24-25	610.0
26-27	1874.0
28-29	4992.0
30-31	15749.0
32-33	20458.0
34-35	28351.0
36-37	44468.0
38-39	51829.0
40-41	49897.0
42-43	57049.0
44-45	68094.0
46-47	68756.0
48-49	64652.0
50-51	59044.0
52-53	56899.0
54-55	62268.0
56-57	64599.0
58-59	64818.0
60-61	66344.0
62-63	63084.0
64-65	68117.0
66-67	73363.0
68-69	74343.0
70-71	77339.0
72-73	65085.0
74-75	65452.0
76-77	67771.0
78-79	69664.0
80-81	68788.0
82-83	64922.0
84-85	63817.0
86-87	66000.0
88-89	68722.0
90-91	69271.0
92-93	67650.0
94-95	76799.0
96-97	230431.0
98-99	498969.0
100-101	5130737.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.9384175180643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.02203505505577	77.36351234387205
2	7.061395594757634	12.136903257650031
3	1.6473178079617974	4.247036566966902
4	0.6038660818435482	2.0758118186587375
5	0.2732592583939152	1.174173411926645
6	0.13703253398840715	0.7065815471672443
7	0.07988378228172617	0.4805560084254391
8	0.04968190570338131	0.3415667484344227
9	0.03417063303358601	0.2642913115647187
>10	0.09046809031272603	1.139769695163348
>50	7.484877022292792E-4	0.04265565456302053
>100	1.4076896529593267E-4	0.027141635607501862
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2687531227186234E-5	0.0
2	0.0	3.8062593681558696E-5	0.0	1.2687531227186234E-5	0.0
3	0.0	3.8062593681558696E-5	0.0	1.2687531227186234E-5	0.0
4	0.0	8.881271859030362E-5	0.0	2.5375062454372467E-5	0.0
5	0.0	8.881271859030362E-5	0.0	2.5375062454372467E-5	0.0
6	7.612518736311739E-5	8.881271859030362E-5	0.0	2.5375062454372467E-5	0.0
7	7.612518736311739E-5	8.881271859030362E-5	0.0	3.8062593681558696E-5	0.0
8	7.612518736311739E-5	1.0150024981748987E-4	0.0	5.0750124908744935E-5	0.0
9	7.612518736311739E-5	2.4106309331653844E-4	0.0	5.0750124908744935E-5	0.0
10-11	1.0150024981748987E-4	2.4106309331653844E-4	0.0	7.612518736311739E-5	0.0
12-13	1.0150024981748987E-4	2.4106309331653844E-4	0.0	8.881271859030362E-5	6.343765613593117E-6
14-15	1.0784401543108299E-4	2.9181321822528336E-4	0.0	1.2053154665826922E-4	2.5375062454372467E-5
16-17	1.7762543718060725E-4	2.9815698383887647E-4	0.0	1.3956284349904855E-4	2.5375062454372467E-5
18-19	1.7762543718060725E-4	3.2987581190684203E-4	0.0	1.5225037472623478E-4	2.5375062454372467E-5
20-21	1.7762543718060725E-4	3.6159463997480765E-4	0.0	1.903129684077935E-4	2.5375062454372467E-5
22-23	1.7762543718060725E-4	4.123447648835526E-4	0.0	2.347193277029453E-4	2.5375062454372467E-5
24-25	1.903129684077935E-4	4.504073585651113E-4	0.0	2.600943901573178E-4	2.5375062454372467E-5
26-27	1.903129684077935E-4	4.8212618663307687E-4	0.0	3.1718828067965583E-4	2.5375062454372467E-5
28-29	2.0300049963497974E-4	4.8212618663307687E-4	0.0	6.470640925864979E-4	2.5375062454372467E-5
30-31	2.1568803086216597E-4	5.392200771554148E-4	0.0	0.0015986289346254655	2.5375062454372467E-5
32-33	2.1568803086216597E-4	5.45563842769008E-4	0.0	0.0036222901653616695	2.5375062454372467E-5
34-35	2.2203179647575907E-4	6.914704518816497E-4	0.0	0.00651504728516013	2.5375062454372467E-5
36-37	2.283755620893522E-4	8.437208266078844E-4	0.0	0.010625807402768469	2.5375062454372467E-5
38-39	2.283755620893522E-4	8.69095889062257E-4	0.0	0.01646841553288773	3.8062593681558696E-5
40-41	2.5375062454372464E-4	9.388773108117812E-4	0.0	0.022659930771754613	3.8062593681558696E-5
42-43	2.5375062454372464E-4	9.642523732661537E-4	0.0	0.028692851870281665	3.8062593681558696E-5
44-45	2.600943901573178E-4	0.0010847839199244229	0.0	0.03577249429505158	3.8062593681558696E-5
46-47	2.664381557709109E-4	0.0011799404041283198	0.0	0.0433152316096138	3.8062593681558696E-5
48-49	2.664381557709109E-4	0.0011799404041283198	0.0	0.0508706564554032	3.8062593681558696E-5
50-51	2.664381557709109E-4	0.001459066091126417	0.0	0.058261143395239176	3.8062593681558696E-5
52-53	2.664381557709109E-4	0.001471753622353603	0.0	0.06495381611757992	3.8062593681558696E-5
54-55	2.664381557709109E-4	0.0014780973879671962	0.0	0.07158939494939832	3.8062593681558696E-5
56-57	2.664381557709109E-4	0.0014971286848079754	0.0	0.07840259921839732	3.8062593681558696E-5
58-59	2.664381557709109E-4	0.0014971286848079754	0.0	0.08442917655131078	3.8062593681558696E-5
60-61	2.7912568699809716E-4	0.001522503747262348	0.0	0.09062069179017768	5.0750124908744935E-5
62-63	3.1718828067965583E-4	0.0015351912784895342	0.0	0.0964886749827513	5.0750124908744935E-5
64-65	3.1718828067965583E-4	0.0017001291844429551	0.0	0.10264212762793662	5.0750124908744935E-5
66-67	3.2987581190684203E-4	0.0017001291844429551	0.0	0.10868139249207727	5.0750124908744935E-5
68-69	3.2987581190684203E-4	0.0017255042468973278	0.0	0.1147333448874451	6.343765613593116E-5
70-71	3.3621957752043513E-4	0.001744535543738107	0.0	0.11955460675377588	6.343765613593116E-5
72-73	3.6793840558840076E-4	0.0017572230749652931	0.0	0.1236717106369978	6.343765613593116E-5
74-75	3.6793840558840076E-4	0.0018016294342604452	0.0	0.12691337486554388	6.343765613593116E-5
76-77	3.7428217120199386E-4	0.0018714108560099695	0.0	0.13034535206249775	6.343765613593116E-5
78-79	3.933134680427732E-4	0.0018904421528507488	0.0	0.13270523287075442	6.343765613593116E-5
80-81	3.933134680427732E-4	0.0018904421528507488	0.0	0.13346014097877199	6.343765613593116E-5
82-83	3.996572336563664E-4	0.0018904421528507488	0.0	0.13396764222785945	6.343765613593116E-5
84-85	4.060009992699595E-4	0.0019031296840779349	0.0	0.13422139285240314	6.343765613593116E-5
86-87	4.060009992699595E-4	0.0019411922777594936	0.0	0.13431020557099346	6.343765613593116E-5
88	4.060009992699595E-4	0.002004629933895425	0.0	0.13433558063344783	6.343765613593116E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11565	0.0	51.664238	1
GTATCAA	20385	0.0	33.750988	1
TCAACGC	25025	0.0	27.83181	4
ATCAACG	25185	0.0	27.638472	3
CAACGCA	25645	0.0	27.17551	5
TATCAAC	26015	0.0	26.855198	2
AACGCAG	26790	0.0	26.06162	6
GTGGTAT	5480	0.0	25.047958	1
TGGTATC	5355	0.0	24.60099	2
ACGCAGA	31495	0.0	22.154816	7
CGCAGAG	32050	0.0	21.797546	8
GTACATG	31415	0.0	19.614681	1
TACATGG	32555	0.0	19.606613	2
TATACAC	7955	0.0	19.49821	3
ACATGGG	32565	0.0	19.026047	3
GCAGAGT	37360	0.0	18.699448	9
CTTATAC	5530	0.0	18.673748	1
GAGTACT	18045	0.0	18.651001	12-13
CAGAGTA	36850	0.0	17.741642	10-11
CATGGGG	21880	0.0	17.304815	4
>>END_MODULE