FastQCFastQC Report
Fri 27 May 2016
SRR2139597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6892471
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104250.050.725081
GTATCAA185800.034.8236431
TCAACGC233550.027.9183774
ATCAACG236150.027.6468243
CAACGCA237550.027.448275
TATCAAC241100.027.2725222
AACGCAG244550.026.7141976
GTGGTAT47900.025.2941741
TGGTATC46850.024.3345452
ACGCAGA278450.023.4014367
CGCAGAG285850.022.8250628
GCAGAGT325350.020.105649
GAGTACT190200.019.61785112-13
CAGAGTA318450.018.81079110-11
AGAGTAC297650.018.16082410-11
GTACTTT208900.017.80202714-15
TACATGG224600.017.7566932
GTACATG223900.017.4176351
TAGACCG4900.017.1700785
ACATGGG223700.017.169053
Produced by FastQC (version 0.11.5)