##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139597_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6892471
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.71123534651071	18.0	18.0	30.0	18.0	33.0
2	29.984556772164876	31.0	28.0	33.0	27.0	33.0
3	31.207635113734973	33.0	31.0	33.0	29.0	33.0
4	32.4680006633325	33.0	33.0	33.0	31.0	34.0
5	32.96939022304193	33.0	33.0	34.0	33.0	34.0
6	37.03680363689597	38.0	38.0	38.0	36.0	38.0
7	37.10436605391593	38.0	38.0	38.0	36.0	38.0
8	37.05690818285634	38.0	38.0	38.0	36.0	38.0
9	37.31779836288031	38.0	38.0	38.0	37.0	38.0
10-11	37.360959226379045	38.0	38.0	38.0	37.0	38.0
12-13	37.391178069519626	38.0	38.0	38.0	37.0	38.0
14-15	37.40422803374871	38.0	38.0	38.0	37.0	38.0
16-17	37.41798674234538	38.0	38.0	38.0	37.0	38.0
18-19	37.45932039467413	38.0	38.0	38.0	37.0	38.0
20-21	37.477088075912064	38.0	38.0	38.0	37.0	38.0
22-23	37.49800019606673	38.0	38.0	38.0	37.0	38.0
24-25	37.511908637107894	38.0	38.0	38.0	37.0	38.0
26-27	37.48596734713708	38.0	38.0	38.0	37.0	38.0
28-29	37.45832307439418	38.0	38.0	38.0	37.0	38.0
30-31	37.46537978246556	38.0	38.0	38.0	37.0	38.0
32-33	37.45282261966092	38.0	38.0	38.0	37.0	38.0
34-35	37.42035338583532	38.0	38.0	38.0	37.0	38.0
36-37	37.40201221381747	38.0	38.0	38.0	37.0	38.0
38-39	37.37377381314186	38.0	38.0	38.0	37.0	38.0
40-41	37.37140921456597	38.0	38.0	38.0	37.0	38.0
42-43	37.34960762337211	38.0	38.0	38.0	37.0	38.0
44-45	37.31000373538828	38.0	38.0	38.0	37.0	38.0
46-47	37.26174671288342	38.0	38.0	38.0	37.0	38.0
48-49	37.202295831048176	38.0	38.0	38.0	37.0	38.0
50-51	37.109750895098855	38.0	38.0	38.0	36.0	38.0
52-53	37.124665541444884	38.0	38.0	38.0	36.0	38.0
54-55	37.10131261544083	38.0	38.0	38.0	36.0	38.0
56-57	37.04588007690565	38.0	38.0	38.0	36.0	38.0
58-59	37.030668244370275	38.0	38.0	38.0	36.0	38.0
60-61	37.047870132578865	38.0	38.0	38.0	36.0	38.0
62-63	37.048499756759746	38.0	38.0	38.0	36.0	38.0
64-65	36.9845767431803	38.0	38.0	38.0	36.0	38.0
66-67	37.02726665214603	38.0	38.0	38.0	36.0	38.0
68-69	37.0402424403555	38.0	38.0	38.0	36.0	38.0
70-71	37.023601144325994	38.0	38.0	38.0	36.0	38.0
72-73	37.01771256933455	38.0	38.0	38.0	36.0	38.0
74-75	36.97461712698034	38.0	38.0	38.0	36.0	38.0
76-77	36.977659925671745	38.0	38.0	38.0	36.0	38.0
78-79	36.977538105115826	38.0	38.0	38.0	36.0	38.0
80-81	36.97355769407618	38.0	38.0	38.0	36.0	38.0
82-83	36.91607838300379	38.0	38.0	38.0	35.0	38.0
84-85	36.9014235373735	38.0	38.0	38.0	35.0	38.0
86-87	36.87128922278472	38.0	38.0	38.0	35.0	38.0
88-89	36.87757475131906	38.0	38.0	38.0	35.0	38.0
90-91	36.865575330111156	38.0	38.0	38.0	35.0	38.0
92-93	36.84420015394588	38.0	38.0	38.0	35.0	38.0
94-95	36.826368237473375	38.0	38.0	38.0	35.0	38.0
96-97	36.8011963599471	38.0	38.0	38.0	35.0	38.0
98-99	36.83562635753565	38.0	38.0	38.0	35.0	38.0
100	35.36530101480236	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	2.0
20	7.0
21	70.0
22	258.0
23	908.0
24	2264.0
25	4742.0
26	9274.0
27	16283.0
28	26468.0
29	39671.0
30	53422.0
31	68106.0
32	88647.0
33	120064.0
34	185158.0
35	350229.0
36	1215760.0
37	4711135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.17255930420309	19.551377147615128	17.256162557666183	23.019900990515595
2	14.657513893983221	20.892076134849408	41.841637609631306	22.60877236153607
3	18.25667239469168	25.592693895387807	31.145773303689207	25.0048604062313
4	12.76753487068344	17.883860435437764	38.178933345481866	31.169671348396932
5	13.574304483834606	36.88681461264037	35.565720914893944	13.973159988631073
6	30.238966547701107	37.42611321832185	18.48775279576802	13.847167438209027
7	26.57645482160679	32.51912275516823	22.809923854285046	18.094498568939922
8	24.243888730181094	36.761489457119225	21.027654668405567	17.966967144294117
9	25.36913104168302	16.95734374508068	21.451566499155383	36.22195871408091
10-11	24.258353789228856	26.66043136053819	28.68617075066402	20.395044099568935
12-13	25.09145123715428	24.224077257633727	28.941957100726285	21.742514404485703
14-15	22.241384838615932	25.601471518704976	26.729209306792878	25.427934335886214
16-17	21.675615319962898	28.74600415438817	27.494392069259344	22.083988456389587
18-19	21.64213676053189	27.6659415759602	29.480095019623587	21.21182664388432
20-21	22.45461819653805	26.737791364490075	29.45536032314064	21.352230115831237
22-23	22.22311700351192	26.742282456963984	29.30058549892775	21.734015040596347
24-25	21.979364604058222	26.882943056938274	29.418183878804616	21.719508460198888
26-27	21.893699248363642	27.013097596765046	29.414366000803895	21.67883715406742
28-29	21.754486938613603	26.906923501783858	29.495374400855834	21.843215158746705
30-31	22.102699942452357	26.877497359189604	29.406730152712758	21.61307254564528
32-33	21.889279390237025	26.99416618086719	29.39155564187674	21.724998787019047
34-35	21.990341224782867	27.02382812654171	29.423575357698173	21.562255290977255
36-37	21.964507263689352	26.867122185981668	29.358718413390644	21.809652136938336
38-39	21.745112303879747	26.93321729242177	29.53874920335214	21.782921200346347
40-41	22.022034057805712	26.76844390813648	29.400647063763536	21.80887497029427
42-43	21.944738319512975	26.86646837756335	29.2682142017351	21.920579101188572
44-45	21.913720525916943	27.06742928891393	29.112454997977206	21.90639518719192
46-47	22.117423651934764	26.96953111136783	28.7267749715764	22.186270265121006
48-49	22.026250480569395	27.090119678577267	28.686073458034496	22.19755638281884
50-51	21.95458832647443	27.282832419189283	28.599031466465103	22.163547787871185
52-53	22.076663015897154	27.242089246184868	28.602910633956714	22.078337103961264
54-55	22.078202907728485	27.184429400953924	28.528938064695463	22.208429626622127
56-57	22.113855290342848	27.339584404532257	28.360139990691913	22.18642031443298
58-59	21.959240644393198	27.357364975060406	28.609037979160362	22.074356401386037
60-61	22.03335082677275	27.498098179091002	28.492340333804805	21.976210660331446
62-63	21.93861414310538	27.576259464331383	28.513823454190046	21.971302938373192
64-65	21.890404476471957	27.48668349217946	28.52551223723973	22.097399794108856
66-67	21.94229291325899	27.492449132321404	28.46329774917024	22.101960205249362
68-69	21.893684404145464	27.46643467377442	28.49267064000309	22.14721028207702
70-71	21.930623135815853	27.126877471000533	28.562930872902896	22.379568520280717
72-73	22.05849131228708	27.077255548813085	28.551902455164285	22.312350683735556
74-75	21.99085999329549	27.301019027445918	28.430603283804995	22.2775176954536
76-77	22.172779717631013	27.20630265234964	28.225926058653073	22.394991571366276
78-79	22.142130317890626	27.28233898616726	28.17335656354337	22.402174132398745
80-81	22.143517936679554	27.30140164124463	28.110263835949944	22.44481658612587
82-83	22.2607461204319	27.29947983557289	28.125636297639588	22.314137746355627
84-85	22.307659550662184	27.349501018725796	28.059941768277152	22.282897662334868
86-87	22.18228447754831	27.440087678192448	28.107830222624923	22.269797621634314
88-89	22.311054280853508	27.371499931465365	28.0380834396134	22.27936234806773
90-91	22.295703378059493	27.425924581875517	28.007821605341487	22.270550434723503
92-93	22.422784999061303	27.42302015882807	28.004088281697303	22.15010656041332
94-95	22.269616212955395	27.47754332847872	28.00156184394389	22.25127861462199
96-97	22.362900477829193	27.57957433764502	28.079923390460344	21.97760179406544
98-99	23.406082005593674	29.45593418756142	28.311582251966083	18.826401554878817
100	29.80734171701525	40.48995964771896	29.70269863526579	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2083.0
1	1932.5
2	1989.0
3	2336.0
4	2901.5
5	4051.5
6	5286.0
7	7522.5
8	9679.0
9	10295.0
10	10023.0
11	9704.5
12	10710.5
13	13450.5
14	19157.0
15	26097.5
16	30889.0
17	32700.5
18	31830.5
19	29223.5
20	26067.0
21	23480.5
22	22967.0
23	25878.0
24	32320.0
25	41672.0
26	53902.5
27	67658.0
28	81275.0
29	96809.0
30	112048.0
31	126715.0
32	144507.0
33	163417.0
34	180411.0
35	197131.5
36	216338.0
37	231641.0
38	242722.0
39	252490.0
40	262399.0
41	271130.0
42	276082.0
43	282012.5
44	291696.0
45	302137.0
46	303099.0
47	300201.0
48	299465.5
49	294574.5
50	287713.0
51	279804.0
52	267178.0
53	251162.5
54	233946.0
55	215470.5
56	195416.0
57	175460.0
58	156356.5
59	134982.5
60	112868.5
61	91112.0
62	71479.5
63	55046.5
64	41212.0
65	30772.5
66	22647.5
67	17143.0
68	13191.0
69	9810.0
70	7142.0
71	4986.5
72	3437.0
73	2313.0
74	1513.5
75	985.5
76	664.0
77	420.5
78	264.5
79	172.5
80	118.5
81	83.0
82	54.5
83	38.0
84	29.0
85	21.5
86	15.0
87	14.0
88	13.0
89	10.5
90	6.5
91	2.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004047895159805533
3	6.238691464933258E-4
4	7.254292401085184E-5
5	0.0
6	0.0
7	4.062403744607703E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.674540205797839E-4
24-25	5.369199586571633E-4
26-27	9.43363366152703E-5
28-29	0.0026569845954421533
30-31	0.00183149038402138
32-33	0.004560308993716098
34-35	0.009230209987642627
36-37	0.0014831802586064288
38-39	0.0
40-41	1.116755524422066E-4
42-43	5.850381883677457E-4
44-45	1.1351235139246736E-4
46-47	5.35176051513906E-5
48-49	0.004139405924200373
50-51	0.003250634692270131
52-53	2.9080318424771026E-4
54-55	0.0027890798017534755
56-57	0.03939313630744453
58-59	0.0611241668323558
60-61	0.02742203919144485
62-63	0.009850897862470282
64-65	8.966146818329593E-4
66-67	1.0906796973246382E-4
68-69	0.0011368744200031527
70-71	0.010001703294363605
72-73	0.015150893867988454
74-75	0.009602027555010445
76-77	0.011689792292435676
78-79	0.0025023422461562137
80-81	0.013409820724850712
82-83	0.043669152079772046
84-85	0.02344824861153997
86-87	0.003422760853293343
88-89	4.742879041407231E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	61.0
20-21	371.0
22-23	773.0
24-25	705.0
26-27	2036.0
28-29	5278.0
30-31	15169.0
32-33	20159.0
34-35	28107.0
36-37	43668.0
38-39	48268.0
40-41	48685.0
42-43	55768.0
44-45	66397.0
46-47	66905.0
48-49	61761.0
50-51	54339.0
52-53	50145.0
54-55	54836.0
56-57	55431.0
58-59	58956.0
60-61	59598.0
62-63	57532.0
64-65	61759.0
66-67	65451.0
68-69	67522.0
70-71	72147.0
72-73	59611.0
74-75	58406.0
76-77	61827.0
78-79	63917.0
80-81	63014.0
82-83	58842.0
84-85	58231.0
86-87	60408.0
88-89	62283.0
90-91	63194.0
92-93	62132.0
94-95	70567.0
96-97	207890.0
98-99	1551114.0
100-101	3269208.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.44067250095449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.33776124427018	79.86635267930603
2	6.253575569146156	10.936337066033582
3	1.3670489051610768	3.586070268269344
4	0.49352153609483024	1.7261542003934416
5	0.2200939800536925	0.9622582814653268
6	0.10569755395438758	0.5545359119686544
7	0.06111525444574007	0.3740771264161723
8	0.04226555959944558	0.29565831640037554
9	0.02614299392738023	0.20573648731786479
>10	0.09013135555598632	1.2370930196333147
>50	0.002017362755058473	0.11946508619438137
>100	5.538141613246231E-4	0.08443305580883854
>500	4.991393999757071E-5	0.028478413420936884
>1k	2.495693472874664E-5	0.02335008737181115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4508584802170369E-5	0.0
2	0.0	0.0	0.0	2.9017169604340738E-5	0.0
3	0.0	0.0	0.0	2.9017169604340738E-5	0.0
4	0.0	2.9017169604340738E-5	0.0	7.254292401085184E-5	0.0
5	0.0	2.9017169604340738E-5	0.0	7.254292401085184E-5	0.0
6	8.705150881302221E-5	2.9017169604340738E-5	0.0	1.0156009361519258E-4	1.4508584802170369E-5
7	8.705150881302221E-5	2.9017169604340738E-5	0.0	1.0156009361519258E-4	2.9017169604340738E-5
8	8.705150881302221E-5	4.3525754406511105E-5	0.0	1.0156009361519258E-4	2.9017169604340738E-5
9	8.705150881302221E-5	8.705150881302221E-5	0.0	1.1606867841736295E-4	7.254292401085184E-5
10-11	1.0156009361519258E-4	8.705150881302221E-5	0.0	1.5959443282387406E-4	7.254292401085184E-5
12-13	1.1606867841736295E-4	1.0156009361519258E-4	0.0	1.8861160242821478E-4	9.430580121410739E-5
14-15	1.1606867841736295E-4	1.4508584802170368E-4	0.0	1.8861160242821478E-4	1.0156009361519258E-4
16-17	1.8861160242821478E-4	1.4508584802170368E-4	0.0	2.0312018723038516E-4	1.0156009361519258E-4
18-19	1.8861160242821478E-4	1.5959443282387406E-4	0.0	2.248830644336407E-4	1.0156009361519258E-4
20-21	1.8861160242821478E-4	1.8861160242821478E-4	0.0	2.974259884444925E-4	1.2332297081844813E-4
22-23	1.8861160242821478E-4	2.1762877203255552E-4	0.0	3.4820603525208884E-4	1.5234014042278887E-4
24-25	1.8861160242821478E-4	2.393916492358111E-4	0.0	3.844774972575147E-4	1.7410301762604442E-4
26-27	1.8861160242821478E-4	2.4664594163689626E-4	0.0	4.8603759087270735E-4	1.8861160242821478E-4
28-29	1.8861160242821478E-4	2.4664594163689626E-4	0.0	9.86583766547585E-4	1.8861160242821478E-4
30-31	1.8861160242821478E-4	2.7566311124123697E-4	0.0	0.002524493755577644	1.9586589482929997E-4
32-33	1.8861160242821478E-4	2.7566311124123697E-4	0.0	0.005767162458862721	2.0312018723038516E-4
34-35	1.8861160242821478E-4	3.191888656477481E-4	0.0	0.009967397759091043	2.0312018723038516E-4
36-37	1.9586589482929997E-4	3.699689124553444E-4	0.0	0.017207181575374056	2.1762877203255552E-4
38-39	2.0312018723038516E-4	3.9173178965859994E-4	0.0	0.03685180539751273	2.1762877203255552E-4
40-41	2.0312018723038516E-4	4.497661288672814E-4	0.0	0.05793277911506628	2.321373568347259E-4
42-43	2.0312018723038516E-4	5.005461756748777E-4	0.0	0.06964120705041776	2.321373568347259E-4
44-45	2.1037447963147033E-4	6.673949008998369E-4	0.0	0.0836782628465176	2.393916492358111E-4
46-47	2.1762877203255552E-4	7.834635793171999E-4	0.0	0.09928950009365292	2.4664594163689626E-4
48-49	2.1762877203255552E-4	7.979721641193703E-4	0.0	0.11453802272073396	2.6115452643906664E-4
50-51	2.3213735683472587E-4	8.632607957291369E-4	0.0	0.12924247341773365	2.7566311124123697E-4
52-53	2.4664594163689626E-4	8.705150881302221E-4	0.0	0.17589482784911248	2.7566311124123697E-4
54-55	2.4664594163689626E-4	8.850236729323925E-4	0.0	0.2337405554553657	2.7566311124123697E-4
56-57	2.4664594163689626E-4	9.140408425367332E-4	0.0	0.3158083653888424	2.7566311124123697E-4
58-59	2.4664594163689626E-4	9.140408425367332E-4	0.0	0.36993989528574	2.7566311124123697E-4
60-61	2.539002340379814E-4	9.430580121410739E-4	0.0	0.38892437849938	3.0468028084557774E-4
62-63	2.9017169604340736E-4	9.793294741465E-4	0.0	0.40505067050699234	3.0468028084557774E-4
64-65	2.9017169604340736E-4	0.0011606867841736294	0.0	0.41292157776216976	3.0468028084557774E-4
66-67	2.9017169604340736E-4	0.0011606867841736294	0.0	0.42031370171887555	3.0468028084557774E-4
68-69	2.9017169604340736E-4	0.00118970395377797	0.0	0.42756073982755965	3.0468028084557774E-4
70-71	2.9017169604340736E-4	0.0012042125385801406	0.0	0.43337868233322996	3.1193457324666296E-4
72-73	2.9017169604340736E-4	0.0012042125385801406	0.0	0.437383051738629	3.191888656477481E-4
74-75	2.9017169604340736E-4	0.0012042125385801406	0.0	0.44074178912033146	3.191888656477481E-4
76-77	2.9017169604340736E-4	0.0012332297081844813	0.0	0.44412954367163826	3.191888656477481E-4
78-79	2.9017169604340736E-4	0.001262246877788822	0.0	0.4464364086551833	3.191888656477481E-4
80-81	2.9017169604340736E-4	0.0012767554625909925	0.0	0.44733594091291784	3.191888656477481E-4
82-83	2.9017169604340736E-4	0.0012767554625909925	0.0	0.44794530147460904	3.4820603525208884E-4
84-85	3.0468028084557774E-4	0.0012767554625909925	0.0	0.4482427274630535	3.4820603525208884E-4
86-87	3.0468028084557774E-4	0.0012840097549920776	0.0	0.4484023218958774	3.4820603525208884E-4
88	3.191888656477481E-4	0.0013347898017996738	0.0	0.4484168304806796	3.627146200542592E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10425	0.0	50.72508	1
GTATCAA	18580	0.0	34.823643	1
TCAACGC	23355	0.0	27.918377	4
ATCAACG	23615	0.0	27.646824	3
CAACGCA	23755	0.0	27.44827	5
TATCAAC	24110	0.0	27.272522	2
AACGCAG	24455	0.0	26.714197	6
GTGGTAT	4790	0.0	25.294174	1
TGGTATC	4685	0.0	24.334545	2
ACGCAGA	27845	0.0	23.401436	7
CGCAGAG	28585	0.0	22.825062	8
GCAGAGT	32535	0.0	20.10564	9
GAGTACT	19020	0.0	19.617851	12-13
CAGAGTA	31845	0.0	18.810791	10-11
AGAGTAC	29765	0.0	18.160824	10-11
GTACTTT	20890	0.0	17.802027	14-15
TACATGG	22460	0.0	17.756693	2
GTACATG	22390	0.0	17.417635	1
TAGACCG	490	0.0	17.170078	5
ACATGGG	22370	0.0	17.16905	3
>>END_MODULE