FastQCFastQC Report
Fri 27 May 2016
SRR2139596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7621338
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99700.050.3940121
GTATCAA175050.035.3049051
TCAACGC223000.028.3235444
ATCAACG226550.027.842253
CAACGCA228850.027.6179775
TATCAAC230200.027.5322862
AACGCAG238000.026.4665976
ACGCAGA274250.022.9527267
GTGGTAT51050.022.5578021
CGCAGAG280350.022.5292228
TGGTATC49250.022.516242
GCAGAGT324700.019.3994739
GTACATG251800.019.3436811
TACATGG256350.019.22
GAGTACT177100.019.19249712-13
ACATGGG254850.018.7427643
CAGAGTA318450.018.0794110-11
AGAGTAC293950.017.77273810-11
GTACTTT194650.017.39656414-15
AGTACTT192000.016.28150212-13
Produced by FastQC (version 0.11.5)