##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139596_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7621338
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.366193311463157	18.0	18.0	30.0	18.0	33.0
2	29.804215086642266	30.0	27.0	33.0	27.0	33.0
3	31.068126751496916	33.0	31.0	33.0	28.0	33.0
4	32.42776937592848	33.0	33.0	33.0	31.0	34.0
5	32.936400537543406	33.0	33.0	34.0	33.0	34.0
6	36.966633811543325	38.0	37.0	38.0	35.0	38.0
7	37.02631965673219	38.0	38.0	38.0	35.0	38.0
8	36.96566088526713	38.0	38.0	38.0	35.0	38.0
9	37.25734208350292	38.0	38.0	38.0	36.0	38.0
10-11	37.30845896088062	38.0	38.0	38.0	36.5	38.0
12-13	37.340592557369845	38.0	38.0	38.0	37.0	38.0
14-15	37.35632483430075	38.0	38.0	38.0	37.0	38.0
16-17	37.37379230523564	38.0	38.0	38.0	37.0	38.0
18-19	37.41936192831233	38.0	38.0	38.0	37.0	38.0
20-21	37.44042661287106	38.0	38.0	38.0	37.0	38.0
22-23	37.469913375164694	38.0	38.0	38.0	37.0	38.0
24-25	37.48440299077671	38.0	38.0	38.0	37.0	38.0
26-27	37.46130165058172	38.0	38.0	38.0	37.0	38.0
28-29	37.436872624911864	38.0	38.0	38.0	37.0	38.0
30-31	37.44434574164188	38.0	38.0	38.0	37.0	38.0
32-33	37.43226392022248	38.0	38.0	38.0	37.0	38.0
34-35	37.40610659542815	38.0	38.0	38.0	37.0	38.0
36-37	37.38920244199689	38.0	38.0	38.0	37.0	38.0
38-39	37.37518788264234	38.0	38.0	38.0	37.0	38.0
40-41	37.35748750884739	38.0	38.0	38.0	37.0	38.0
42-43	37.33730408659585	38.0	38.0	38.0	37.0	38.0
44-45	37.30385492775938	38.0	38.0	38.0	37.0	38.0
46-47	37.260915276455584	38.0	38.0	38.0	37.0	38.0
48-49	37.20582357001558	38.0	38.0	38.0	37.0	38.0
50-51	37.115624301946525	38.0	38.0	38.0	36.0	38.0
52-53	37.12836175768986	38.0	38.0	38.0	36.0	38.0
54-55	37.099369022028654	38.0	38.0	38.0	36.0	38.0
56-57	37.03931024945237	38.0	38.0	38.0	36.0	38.0
58-59	37.02130123654345	38.0	38.0	38.0	36.0	38.0
60-61	37.037729452125376	38.0	38.0	38.0	36.0	38.0
62-63	37.03103220841285	38.0	38.0	38.0	36.0	38.0
64-65	36.96641133348609	38.0	38.0	38.0	36.0	38.0
66-67	37.00486830116671	38.0	38.0	38.0	36.0	38.0
68-69	37.01149770119696	38.0	38.0	38.0	36.0	38.0
70-71	36.986733350662725	38.0	38.0	38.0	36.0	38.0
72-73	36.97756282782143	38.0	38.0	38.0	36.0	38.0
74-75	36.92939514154334	38.0	38.0	38.0	35.5	38.0
76-77	36.92790852973217	38.0	38.0	38.0	35.0	38.0
78-79	36.924643597396894	38.0	38.0	38.0	35.0	38.0
80-81	36.91787068243257	38.0	38.0	38.0	35.0	38.0
82-83	36.85902970190337	38.0	38.0	38.0	35.0	38.0
84-85	36.84130014743643	38.0	38.0	38.0	35.0	38.0
86-87	36.80404009770521	38.0	38.0	38.0	35.0	38.0
88-89	36.80794603708253	38.0	38.0	38.0	35.0	38.0
90-91	36.790116496988816	38.0	38.0	38.0	35.0	38.0
92-93	36.76759121732389	38.0	38.0	38.0	35.0	38.0
94-95	36.746064227066555	38.0	38.0	38.0	34.5	38.0
96-97	36.72044883276924	38.0	38.0	38.0	34.0	38.0
98-99	36.75522949304353	38.0	38.0	38.0	34.0	38.0
100	35.15778031253244	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	7.0
20	22.0
21	81.0
22	347.0
23	1128.0
24	2690.0
25	5701.0
26	10480.0
27	17949.0
28	29186.0
29	42722.0
30	58565.0
31	76380.0
32	100637.0
33	140502.0
34	218983.0
35	418531.0
36	1417725.0
37	5079700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18247189666697	19.28502580518014	17.639855363979397	22.8926469341735
2	14.93204968895199	20.87273209194057	41.3943952310783	22.800822988029147
3	18.64831193164932	25.243643296373246	30.58669240160944	25.52135237036799
4	13.101425459673646	17.558166677185387	37.73247743734001	31.60793042580095
5	14.021291274576722	36.83703570160515	35.054920802620224	14.086752221197903
6	31.04583473400602	36.82836268382271	18.292444187621648	13.833358394549618
7	27.372754336825167	32.190501333040125	22.43464731284068	18.002097017294027
8	24.615940140694455	36.68197106597293	20.683139889609933	18.018948903722677
9	25.753575028426766	17.00593780252234	21.157780956572193	36.0827062124787
10-11	24.70280152907534	26.510614015544252	28.298206955261662	20.488377500118744
12-13	25.434457834044366	24.049300792065644	28.57623556388655	21.94000581000344
14-15	22.548442281394685	25.42152703370458	26.365685395399076	25.66434528950166
16-17	22.067509668249855	28.483200718823916	27.116839589059033	22.3324500238672
18-19	22.131796280390663	27.60584821195438	28.86368509046574	21.398670417189216
20-21	22.97208365685299	26.730363545886977	28.77227857232128	21.525274224938748
22-23	22.70109970931825	26.576906661995288	28.637640481689935	22.084353146996524
24-25	22.42245964287432	26.71145057842004	28.73392442007905	22.13216535862659
26-27	22.31501442202826	26.85019700524726	28.733269777891053	22.101518794833424
28-29	22.253162853518322	26.844991047676253	28.784902985037885	22.116943113767544
30-31	22.499759179341016	26.742171554460885	28.673178702596275	22.08489056360182
32-33	22.267854584414025	26.922133858947916	28.62861447813931	22.181397078498755
34-35	22.465189530544695	26.871046020513205	28.63236918248325	22.03139526645886
36-37	22.42543491782096	26.776114541235884	28.597100806381853	22.20134973456131
38-39	22.282021930204426	26.922969988600787	28.709167063875675	22.08584101731911
40-41	22.54980345394483	26.819911263411434	28.52562076763067	22.104664515013063
42-43	22.400305407661893	26.89666066057711	28.38017446135804	22.32285947040296
44-45	22.46075175938277	26.980607672496692	28.289067254045253	22.269573314075288
46-47	22.5397226301823	26.972136458473468	28.030960238330632	22.457180673013607
48-49	22.492378281672767	27.055328052434014	28.014506433242296	22.437787232650923
50-51	22.4721378252613	27.233600471807947	27.89160684711141	22.402654855819346
52-53	22.670418463898454	27.115813971721607	27.764312981867324	22.44945458251262
54-55	22.610924520677433	27.0441189411023	27.837030787811546	22.507925750408724
56-57	22.554489117837257	27.16693772763168	27.82104527200812	22.457527882522943
58-59	22.543537837520482	27.113283342801164	27.845102438661296	22.498076381017064
60-61	22.560948735817465	27.10341084845097	27.91785704467414	22.417783371057425
62-63	22.57011348451159	27.131292652927296	27.912475230827603	22.386118631733503
64-65	22.544498522760623	27.080008278485412	27.898074364844742	22.477418833909226
66-67	22.567769128409797	27.11989626827399	27.776800689296334	22.53553391401988
68-69	22.523638843004782	27.129349232568696	27.874732307831394	22.472279616595124
70-71	22.5089385590059	26.884173821787627	27.921601367318566	22.685286251887902
72-73	22.576592868216483	26.92224038686777	27.853131734194047	22.6480350107217
74-75	22.47453143003412	27.113786429629734	27.79546328848038	22.616218851855766
76-77	22.601857475175564	26.99632225928918	27.713445678466737	22.688374587068523
78-79	22.561194838854824	27.008426267450435	27.653502174578048	22.776876719116693
80-81	22.554040683671165	27.106494316398795	27.618140352069464	22.72132464786058
82-83	22.676200839924242	27.058572047970962	27.639576894122914	22.625650217981878
84-85	22.675074300954	27.101292355869532	27.593555050285673	22.630078292890794
86-87	22.57819547651133	27.1211885519529	27.661195128713	22.639420842822773
88-89	22.639788739317744	27.108016155150228	27.602733471680995	22.649461633851033
90-91	22.642416235408707	27.121949246653713	27.594802323484757	22.640832194452823
92-93	22.693242115628276	27.101890479948885	27.59670511067834	22.6081622937445
94-95	22.55800528804224	27.136373806730806	27.629085180763873	22.67653572446308
96-97	22.714658205992297	27.26540253206715	27.63159255542457	22.388346706515975
98-99	23.80024064329876	29.160870785019565	27.848183025485312	19.190705546196362
100	22.72543192614293	29.98946499565609	21.6764892313864	25.60861384681457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2035.0
1	1839.0
2	1803.5
3	2079.0
4	2552.5
5	3425.5
6	4377.0
7	6373.5
8	8449.0
9	9132.0
10	8951.5
11	8665.0
12	9478.0
13	11889.5
14	16801.0
15	22751.5
16	27031.5
17	28796.5
18	28194.5
19	26099.0
20	23322.5
21	21099.0
22	20897.0
23	23390.5
24	29087.0
25	37711.0
26	48672.0
27	61373.0
28	74964.0
29	90698.0
30	107304.0
31	123687.5
32	142554.0
33	162302.0
34	181454.5
35	200335.0
36	221330.5
37	240106.0
38	254280.5
39	267053.0
40	279238.5
41	289796.5
42	296540.0
43	304615.5
44	313377.5
45	321209.0
46	326944.5
47	328789.0
48	328542.0
49	326651.5
50	323295.5
51	316770.5
52	307105.5
53	293229.5
54	276308.5
55	257899.5
56	236363.5
57	213804.5
58	191044.0
59	166774.5
60	141264.0
61	115507.5
62	91740.0
63	71671.5
64	54442.5
65	40302.5
66	29742.5
67	22641.0
68	17330.0
69	12885.5
70	9554.5
71	6888.5
72	4741.5
73	3116.0
74	2089.0
75	1344.5
76	824.5
77	501.5
78	312.0
79	200.5
80	134.5
81	86.0
82	56.5
83	35.0
84	23.5
85	20.5
86	16.0
87	11.5
88	8.0
89	5.0
90	3.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00430370625210429
3	6.954159492729492E-4
4	9.184738952661592E-5
5	0.0
6	0.0
7	4.8547905892639846E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.495272819172786E-4
24-25	4.5929314391471684E-4
26-27	8.530630969432059E-5
28-29	0.00275700994394408
30-31	0.002142042378271543
32-33	0.004463907725891837
34-35	0.008892846546703685
36-37	0.001584248198000533
38-39	0.0
40-41	1.00644790917492E-4
42-43	6.888237867871578E-4
44-45	2.2459503473464302E-4
46-47	6.18034239096846E-5
48-49	0.004378732358367927
50-51	0.0033671489630194133
52-53	3.6597272841607214E-4
54-55	0.0028787207021923017
56-57	0.040187108087237335
58-59	0.06302062929086115
60-61	0.028944696435134542
62-63	0.010359360573050794
64-65	8.577135324051936E-4
66-67	1.1938954038683255E-4
68-69	0.0012732285097108913
70-71	0.010240752794238092
72-73	0.015923860441535695
74-75	0.010195926157613666
76-77	0.012346876612111011
78-79	0.0026341527314977273
80-81	0.01398168263674869
82-83	0.04442116531604459
84-85	0.023378092793158223
86-87	0.0033467977369382356
88-89	1.3330413972673316E-4
90-91	8.425749055410338E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	250.0
22-23	543.0
24-25	620.0
26-27	1966.0
28-29	4955.0
30-31	14414.0
32-33	19210.0
34-35	26562.0
36-37	41973.0
38-39	47166.0
40-41	47072.0
42-43	54267.0
44-45	64629.0
46-47	65212.0
48-49	61087.0
50-51	54285.0
52-53	51404.0
54-55	56786.0
56-57	57651.0
58-59	59362.0
60-61	57849.0
62-63	57177.0
64-65	60422.0
66-67	63557.0
68-69	64939.0
70-71	63686.0
72-73	60511.0
74-75	61622.0
76-77	64757.0
78-79	66329.0
80-81	65611.0
82-83	62577.0
84-85	61522.0
86-87	63761.0
88-89	66311.0
90-91	67988.0
92-93	66380.0
94-95	77739.0
96-97	238630.0
98-99	519975.0
100-101	4980539.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.44278688721312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.12301604385797	79.68050472443176
2	6.401624305373462	11.195517397335507
3	1.4273727285027757	3.744403479212645
4	0.5077156476198094	1.775842846964895
5	0.21487221644581936	0.9394512715327455
6	0.11555869145638908	0.6062864417991748
7	0.06627012639464297	0.405639117752081
8	0.03862444445800691	0.2701943252302799
9	0.025792132600739967	0.20298023589359224
>10	0.07745472377607082	1.0317841118465993
>50	0.001421889343826685	0.08526677401575168
>100	2.5492344793635766E-4	0.04622503481837948
>500	2.2126722533970508E-5	0.015904239166674942
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3121055646659418E-5
2	0.0	2.6242111293318837E-5	0.0	0.0	1.3121055646659418E-5
3	0.0	2.6242111293318837E-5	0.0	0.0	1.3121055646659418E-5
4	0.0	6.56052782332971E-5	0.0	0.0	1.3121055646659418E-5
5	0.0	9.184738952661594E-5	0.0	0.0	1.3121055646659418E-5
6	1.3121055646659418E-5	9.184738952661594E-5	0.0	0.0	2.6242111293318837E-5
7	1.3121055646659418E-5	9.184738952661594E-5	0.0	2.6242111293318837E-5	2.6242111293318837E-5
8	1.3121055646659418E-5	9.184738952661594E-5	0.0	2.6242111293318837E-5	2.6242111293318837E-5
9	1.3121055646659418E-5	1.443316121132536E-4	0.0	2.6242111293318837E-5	2.6242111293318837E-5
10-11	1.3121055646659418E-5	1.508921399365833E-4	0.0	2.6242111293318837E-5	2.6242111293318837E-5
12-13	2.6242111293318837E-5	1.5745266775991302E-4	0.0	2.6242111293318837E-5	2.6242111293318837E-5
14-15	2.6242111293318837E-5	2.099368903465507E-4	0.0	2.6242111293318837E-5	3.9363166939978255E-5
16-17	2.6242111293318837E-5	2.099368903465507E-4	0.0	3.9363166939978255E-5	5.2484222586637674E-5
18-19	3.280263911664855E-5	2.2305794599321012E-4	0.0	5.2484222586637674E-5	5.2484222586637674E-5
20-21	3.9363166939978255E-5	2.624211129331884E-4	0.0	5.2484222586637674E-5	5.2484222586637674E-5
22-23	3.9363166939978255E-5	3.60829030283134E-4	0.0	5.2484222586637674E-5	5.9044750409967386E-5
24-25	8.528686170328622E-5	4.264343085164311E-4	0.0	5.2484222586637674E-5	6.56052782332971E-5
26-27	1.1808950081993477E-4	4.5923694763307966E-4	0.0	1.2465002864326449E-4	6.56052782332971E-5
28-29	1.1808950081993477E-4	4.5923694763307966E-4	0.0	3.870711415764529E-4	6.56052782332971E-5
30-31	1.312105564665942E-4	4.854790589263985E-4	0.0	0.0014826792880725144	6.56052782332971E-5
32-33	1.508921399365833E-4	4.986001145730579E-4	0.0	0.004349629946867597	7.872633387995651E-5
34-35	1.7057372340657245E-4	5.838869762763441E-4	0.0	0.008049767639225554	9.184738952661594E-5
36-37	1.8369477905323187E-4	6.560527823329709E-4	0.0	0.013816471595932366	1.0496844517327535E-4
38-39	1.8369477905323187E-4	6.822948936262898E-4	0.0	0.02710154043817503	1.0496844517327535E-4
40-41	1.8369477905323187E-4	7.216580605662681E-4	0.0	0.041672472733790315	1.1808950081993477E-4
42-43	1.8369477905323187E-4	7.413396440362572E-4	0.0	0.05091625643686187	1.2465002864326449E-4
44-45	1.9025530687656156E-4	8.266265057395434E-4	0.0	0.06166240101147594	1.377710842899239E-4
46-47	2.2961847381653983E-4	8.922317839728405E-4	0.0	0.07408148018103908	1.443316121132536E-4
48-49	2.3617900163986954E-4	8.987923117961702E-4	0.0	0.08537870909281284	1.443316121132536E-4
50-51	2.3617900163986954E-4	9.972002291461159E-4	0.0	0.09699084334010642	1.443316121132536E-4
52-53	2.7554216857984777E-4	0.0010103212847927753	0.0	0.12503709978484093	1.5745266775991302E-4
54-55	2.7554216857984777E-4	0.0010562449795560832	0.0	0.15896158915927885	1.9025530687656156E-4
56-57	2.7554216857984777E-4	0.0010890476186727316	0.0	0.20367158627527082	1.9681583469989127E-4
58-59	2.7554216857984777E-4	0.0010890476186727316	0.0	0.24104035275695684	2.0337636252322098E-4
60-61	3.017842798731666E-4	0.00112841078561271	0.0	0.2537284135672765	2.099368903465507E-4
62-63	3.0834480769649633E-4	0.001194016063846007	0.0	0.2678204273317887	2.3617900163986954E-4
64-65	3.1490533551982604E-4	0.0013121055646659419	0.0	0.27468273943499155	2.3617900163986954E-4
66-67	3.1490533551982604E-4	0.0013121055646659419	0.0	0.28088243822803816	2.3617900163986954E-4
68-69	3.2802639116648547E-4	0.0013711503150759092	0.0	0.2874888897461312	2.3617900163986954E-4
70-71	3.2802639116648547E-4	0.0014039529541925578	0.0	0.29284884097779157	2.3617900163986954E-4
72-73	3.2802639116648547E-4	0.0014236345376625469	0.0	0.2977889184287588	2.886632242265072E-4
74-75	3.2802639116648547E-4	0.0014498766489558657	0.0	0.30130536134206354	2.886632242265072E-4
76-77	3.2802639116648547E-4	0.0014761187602491846	0.0	0.3048218042553683	2.886632242265072E-4
78-79	3.2802639116648547E-4	0.0014958003437191738	0.0	0.3069998994927138	2.886632242265072E-4
80-81	3.2802639116648547E-4	0.0015023608715425034	0.0	0.3076953154419867	2.952237520498369E-4
82-83	3.411474468131449E-4	0.0015154819271891628	0.0	0.30845633666949296	3.0834480769649633E-4
84-85	3.411474468131449E-4	0.0015417240384824815	0.0	0.30867939461548616	3.1490533551982604E-4
86-87	3.411474468131449E-4	0.0015876477332457896	0.0	0.3087974841163061	3.1490533551982604E-4
88	3.411474468131449E-4	0.0016138898445391084	0.0	0.3088171656997761	3.1490533551982604E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9970	0.0	50.394012	1
GTATCAA	17505	0.0	35.304905	1
TCAACGC	22300	0.0	28.323544	4
ATCAACG	22655	0.0	27.84225	3
CAACGCA	22885	0.0	27.617977	5
TATCAAC	23020	0.0	27.532286	2
AACGCAG	23800	0.0	26.466597	6
ACGCAGA	27425	0.0	22.952726	7
GTGGTAT	5105	0.0	22.557802	1
CGCAGAG	28035	0.0	22.529222	8
TGGTATC	4925	0.0	22.51624	2
GCAGAGT	32470	0.0	19.399473	9
GTACATG	25180	0.0	19.343681	1
TACATGG	25635	0.0	19.2	2
GAGTACT	17710	0.0	19.192497	12-13
ACATGGG	25485	0.0	18.742764	3
CAGAGTA	31845	0.0	18.07941	10-11
AGAGTAC	29395	0.0	17.772738	10-11
GTACTTT	19465	0.0	17.396564	14-15
AGTACTT	19200	0.0	16.281502	12-13
>>END_MODULE