FastQCFastQC Report
Fri 27 May 2016
SRR2139595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7408376
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105250.051.03571
GTATCAA194850.037.46791
TCAACGC245600.030.0731284
ATCAACG247750.029.8294533
CAACGCA252850.029.1597795
TATCAAC256600.029.1029242
AACGCAG260400.028.2978616
TGGTATC49800.024.9639762
ACGCAGA299100.024.6364547
GTGGTAT51800.024.2483941
CGCAGAG309850.023.7537218
GCAGAGT348900.021.1565599
TACATGG276350.020.3525812
GTACATG278550.020.1302741
ACATGGG276500.019.8133263
GAGTACT196750.019.08544212-13
AGAGTAC324250.018.71958410-11
CAGAGTA346450.018.57794610-11
GTACTTT216600.017.21804814-15
CATGGGG182950.017.1514324
Produced by FastQC (version 0.11.5)