##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139595_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7408376
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.91848280918787	18.0	18.0	30.0	18.0	33.0
2	30.095388382015166	31.0	29.0	33.0	27.0	33.0
3	31.333996816576263	33.0	31.0	33.0	29.0	33.0
4	32.51162656970975	33.0	33.0	33.0	31.0	34.0
5	33.00346837147575	33.0	33.0	34.0	33.0	34.0
6	37.06490572292767	38.0	38.0	38.0	36.0	38.0
7	37.157979697574746	38.0	38.0	38.0	36.0	38.0
8	37.10696230860852	38.0	38.0	38.0	36.0	38.0
9	37.34457079392298	38.0	38.0	38.0	37.0	38.0
10-11	37.382222028147595	38.0	38.0	38.0	37.0	38.0
12-13	37.40489784805739	38.0	38.0	38.0	37.0	38.0
14-15	37.42671814983473	38.0	38.0	38.0	37.0	38.0
16-17	37.443537558028915	38.0	38.0	38.0	37.0	38.0
18-19	37.46971421266955	38.0	38.0	38.0	37.0	38.0
20-21	37.48441541216313	38.0	38.0	38.0	37.0	38.0
22-23	37.50392902536136	38.0	38.0	38.0	37.0	38.0
24-25	37.51907776840299	38.0	38.0	38.0	37.5	38.0
26-27	37.4933712032186	38.0	38.0	38.0	37.0	38.0
28-29	37.46712536974621	38.0	38.0	38.0	37.0	38.0
30-31	37.4756394114012	38.0	38.0	38.0	37.0	38.0
32-33	37.462940473300286	38.0	38.0	38.0	37.0	38.0
34-35	37.43363671027899	38.0	38.0	38.0	37.0	38.0
36-37	37.41553650061897	38.0	38.0	38.0	37.0	38.0
38-39	37.40471322752705	38.0	38.0	38.0	37.0	38.0
40-41	37.38994638697689	38.0	38.0	38.0	37.0	38.0
42-43	37.36698089358238	38.0	38.0	38.0	37.0	38.0
44-45	37.330231780133914	38.0	38.0	38.0	37.0	38.0
46-47	37.28591409585773	38.0	38.0	38.0	37.0	38.0
48-49	37.226455361371535	38.0	38.0	38.0	37.0	38.0
50-51	37.14330876104156	38.0	38.0	38.0	37.0	38.0
52-53	37.16433956875291	38.0	38.0	38.0	37.0	38.0
54-55	37.14671729040913	38.0	38.0	38.0	36.5	38.0
56-57	37.09571606503813	38.0	38.0	38.0	36.0	38.0
58-59	37.082160526531766	38.0	38.0	38.0	36.0	38.0
60-61	37.1044292245966	38.0	38.0	38.0	36.0	38.0
62-63	37.10658576942201	38.0	38.0	38.0	36.0	38.0
64-65	37.049139645391364	38.0	38.0	38.0	36.0	38.0
66-67	37.09258547115839	38.0	38.0	38.0	36.0	38.0
68-69	37.10389581246831	38.0	38.0	38.0	36.0	38.0
70-71	37.086881001703276	38.0	38.0	38.0	36.0	38.0
72-73	37.082238569630334	38.0	38.0	38.0	36.0	38.0
74-75	37.040401725407506	38.0	38.0	38.0	36.0	38.0
76-77	37.04591934060814	38.0	38.0	38.0	36.0	38.0
78-79	37.04833681700727	38.0	38.0	38.0	36.0	38.0
80-81	37.045607130966445	38.0	38.0	38.0	36.0	38.0
82-83	36.99504210290905	38.0	38.0	38.0	36.0	38.0
84-85	36.98057814570002	38.0	38.0	38.0	36.0	38.0
86-87	36.95308607917035	38.0	38.0	38.0	36.0	38.0
88-89	36.96137202149814	38.0	38.0	38.0	36.0	38.0
90-91	36.94739805358746	38.0	38.0	38.0	35.5	38.0
92-93	36.93056819988077	38.0	38.0	38.0	35.0	38.0
94-95	36.91587479000803	38.0	38.0	38.0	35.0	38.0
96-97	36.89612643387864	38.0	38.0	38.0	35.0	38.0
98-99	36.92929624858571	38.0	38.0	38.0	35.0	38.0
100	35.45551610409646	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	8.0
21	59.0
22	302.0
23	928.0
24	2576.0
25	5105.0
26	9616.0
27	17036.0
28	28003.0
29	41457.0
30	55689.0
31	71876.0
32	92723.0
33	124890.0
34	189579.0
35	352316.0
36	1174504.0
37	5241704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61656697770199	19.601245941080744	17.033625183171047	22.74856189804621
2	14.723649198817288	21.064644829515146	41.724864633706474	22.486841337961096
3	18.25591672925399	25.88705719356464	30.899464871638845	24.95756120554253
4	12.748474056840312	17.778434092578273	38.411356129804005	31.061735720777406
5	13.513312499257596	37.3402213926507	35.328336466723606	13.818129641368095
6	30.232928242303036	37.457858510421175	18.60973309130098	13.699480155974806
7	26.812962325792412	32.635326364332265	22.869739486532122	17.681971823343208
8	24.47972403128567	36.62999826142734	20.959276365022507	17.931001342264487
9	25.756765045402663	16.910534778472368	21.424978969749915	35.90772120637505
10-11	24.348595427661877	26.756336341459992	28.635513370271703	20.25955486060643
12-13	25.212921158429324	24.411072008224206	28.843102725887565	21.532904107458908
14-15	22.339065943737197	25.66103556298978	26.776077510104777	25.223820983168245
16-17	21.685616388801	28.7704282288048	27.47273491518249	22.07122046721171
18-19	21.693951548895466	27.808152555971784	29.40581714535007	21.09207874978268
20-21	22.6489934012433	26.893238985868468	29.324906916772083	21.132860696116154
22-23	22.380011047617607	26.754801474959542	29.198175685197974	21.667011792224873
24-25	22.041802180821936	26.89337262627901	29.307095646381846	21.757729546517208
26-27	21.90960310710798	27.042185387697913	29.30609730922796	21.742114195966145
28-29	21.88782519300759	27.02542497864775	29.34422392608043	21.74252590226423
30-31	22.144001803651747	26.933829878226472	29.214832981091394	21.707335337030386
32-33	21.88495758370184	27.12084029541217	29.175773136865786	21.818428984020205
34-35	22.057680899509556	27.098726919883887	29.17538940687357	21.66820277373299
36-37	22.061707807497175	26.955407877331407	29.123162681217856	21.85972163395356
38-39	21.885596256035722	27.143084123008226	29.21318122417844	21.75813839677761
40-41	22.147871943074556	26.995181529397872	29.07421011735698	21.78273641017059
42-43	22.046454810031527	27.071588993975134	28.86495783284712	22.01699836314622
44-45	22.072131913554784	27.200500389831785	28.789575453464344	21.937792243149087
46-47	22.183310085854256	27.16476012973232	28.50960207803366	22.14232770637977
48-49	22.123088988934807	27.26523792814653	28.482857554528103	22.12881552839056
50-51	22.12269990442896	27.474106861437985	28.265923049062636	22.137270185070417
52-53	22.300630773911898	27.363956331368865	28.17382716262515	22.161585732094085
54-55	22.287504283161596	27.305098123437222	28.187445715523296	22.21995187787789
56-57	22.2274015227046	27.427423266025	28.192041447912253	22.153133763358152
58-59	22.16066720785895	27.407783767502853	28.23696765710552	22.194581367532678
60-61	22.231742535094455	27.399029730110268	28.250570476158227	22.118657258637047
62-63	22.211623638495706	27.430402855285895	28.254243366899868	22.10373013931853
64-65	22.183405171243027	27.339558313047498	28.27012884950496	22.206907666204515
66-67	22.265447647266527	27.337352635666296	28.152219259084703	22.244980457982475
68-69	22.174113623242295	27.338044491597852	28.274309411471414	22.213532473688435
70-71	22.18047507043426	27.075589133246126	28.347396913172414	22.3965388831472
72-73	22.262252842501603	27.133415816717445	28.29067319956367	22.313658141217285
74-75	22.09015960182741	27.398060872798446	28.176483425040267	22.33529610033388
76-77	22.268103141455054	27.26460065334046	28.1306349099236	22.336661295280884
78-79	22.228654112797905	27.259144496785797	28.084572244364576	22.42762914605172
80-81	22.225173425006993	27.34982487524677	28.0223462686397	22.402655431106535
82-83	22.35251011314076	27.30647589964815	28.04848480658618	22.292529180624907
84-85	22.377979118680038	27.298417104125182	27.998683177972605	22.32492059922217
86-87	22.247767759338544	27.38739832218703	28.02962655558471	22.335207362889715
88-89	22.347809969287358	27.352575285420595	27.95381524315837	22.34579950213368
90-91	22.38436999542807	27.438688498716342	27.906123558833396	22.2708179470222
92-93	22.424507515892447	27.402188949346996	27.89397470707044	22.27932882769012
94-95	22.315055845279698	27.424332849452078	27.88463732602655	22.37597397924167
96-97	22.43239123829226	27.546672424493845	27.91893343207971	22.10200290513418
98-99	23.483092815376498	29.472961340964222	28.129595600635724	18.914350243023552
100	22.415142029325494	30.302732251119807	22.052523929495653	25.229601790059046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1702.0
1	1629.5
2	2022.0
3	2642.5
4	3269.0
5	4388.0
6	5486.5
7	7569.5
8	9904.5
9	10899.0
10	10806.5
11	10604.0
12	11476.5
13	14004.0
14	19641.5
15	26362.0
16	30820.5
17	32430.5
18	31248.5
19	28351.5
20	25165.5
21	22754.0
22	22651.5
23	25650.5
24	31680.0
25	40978.0
26	53427.0
27	67448.0
28	81298.5
29	97641.5
30	114450.0
31	130328.0
32	149082.0
33	169413.0
34	189139.5
35	207770.5
36	227887.5
37	244828.0
38	256681.0
39	267340.0
40	278352.0
41	286716.5
42	290402.0
43	295944.0
44	300996.0
45	305462.5
46	307546.5
47	306151.5
48	306053.0
49	304412.0
50	298827.0
51	290390.0
52	279553.0
53	265305.5
54	248473.5
55	230012.5
56	210724.0
57	190807.5
58	170541.0
59	148975.5
60	126274.0
61	103128.0
62	81369.0
63	63020.5
64	47811.0
65	35651.0
66	26294.5
67	19873.5
68	15048.0
69	11059.0
70	8056.0
71	5764.5
72	4004.5
73	2673.0
74	1743.5
75	1138.0
76	713.0
77	420.0
78	271.0
79	169.5
80	109.0
81	69.5
82	41.0
83	29.0
84	25.5
85	21.5
86	16.5
87	12.5
88	9.5
89	7.0
90	5.0
91	3.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0044274210704208315
3	8.098940982477132E-4
4	9.448764479556653E-5
5	0.0
6	0.0
7	6.074205736857849E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.467082929910984E-4
24-25	4.7924582097644107E-4
26-27	8.100702533427212E-5
28-29	0.0029441197959643953
30-31	0.0019463488927707738
32-33	0.004535681290808862
34-35	0.009302636758090121
36-37	0.001525068691068265
38-39	0.0
40-41	8.945513223016805E-5
42-43	6.090581621476166E-4
44-45	1.3939969195456072E-4
46-47	3.514179821003142E-5
48-49	0.004202593049520579
50-51	0.003385332952138177
52-53	2.733418078884719E-4
54-55	0.0029489454223382543
56-57	0.042263244582954086
58-59	0.06409592313849571
60-61	0.02990355139565249
62-63	0.010574181022944626
64-65	8.223919367564002E-4
66-67	1.0656018766771337E-4
68-69	0.0012906414741645459
70-71	0.011277828371153487
72-73	0.016699039159364264
74-75	0.010459832898639321
76-77	0.01264915394227711
78-79	0.002795578667462856
80-81	0.01426361986410971
82-83	0.044661230703596594
84-85	0.023772609217939323
86-87	0.003701587578177152
88-89	1.8662529466649508E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	54.0
20-21	224.0
22-23	538.0
24-25	558.0
26-27	1585.0
28-29	4122.0
30-31	11914.0
32-33	15651.0
34-35	21866.0
36-37	34411.0
38-39	41240.0
40-41	40845.0
42-43	47605.0
44-45	58544.0
46-47	59696.0
48-49	56144.0
50-51	50351.0
52-53	49218.0
54-55	53455.0
56-57	55664.0
58-59	55854.0
60-61	54172.0
62-63	53720.0
64-65	57009.0
66-67	60197.0
68-69	61913.0
70-71	60736.0
72-73	57704.0
74-75	58802.0
76-77	62325.0
78-79	64447.0
80-81	64261.0
82-83	61332.0
84-85	59975.0
86-87	62186.0
88-89	64532.0
90-91	65655.0
92-93	65400.0
94-95	75279.0
96-97	227447.0
98-99	501069.0
100-101	4910676.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.94533499701684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82274815598075	78.9661426377144
2	6.649920164222964	11.563590727635658
3	1.4604487887829385	3.8093762756016023
4	0.49973523317388985	1.7379858903246472
5	0.21468725771077454	0.933302777062709
6	0.1168215512322314	0.6094253344054514
7	0.07131316169998841	0.4340242713591347
8	0.04171664681618714	0.2901654265908502
9	0.028782144141589933	0.22522258478906662
>10	0.09168438016334204	1.228288714179421
>50	0.0016730999188509104	0.09389682486136326
>100	4.223415350005565E-4	0.0703097487834203
>500	2.3537318525981177E-5	0.01397732210170107
>1k	2.3537302893089938E-5	0.024291464590545927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.6996469941590437E-5	0.0	1.3498234970795218E-5	0.0
5	0.0	2.6996469941590437E-5	0.0	1.3498234970795218E-5	0.0
6	1.3498234970795218E-5	2.6996469941590437E-5	0.0	1.3498234970795218E-5	1.3498234970795218E-5
7	1.3498234970795218E-5	2.6996469941590437E-5	0.0	1.3498234970795218E-5	1.3498234970795218E-5
8	1.3498234970795218E-5	2.6996469941590437E-5	0.0	1.3498234970795218E-5	1.3498234970795218E-5
9	1.3498234970795218E-5	6.74911748539761E-5	0.0	1.3498234970795218E-5	1.3498234970795218E-5
10-11	1.3498234970795218E-5	6.74911748539761E-5	0.0	2.6996469941590437E-5	2.6996469941590437E-5
12-13	1.3498234970795218E-5	6.74911748539761E-5	0.0	2.6996469941590437E-5	2.6996469941590437E-5
14-15	1.3498234970795218E-5	1.417314671933498E-4	0.0	2.6996469941590437E-5	2.6996469941590437E-5
16-17	4.0494704912385654E-5	1.484805846787474E-4	0.0	2.6996469941590437E-5	2.6996469941590437E-5
18-19	4.0494704912385654E-5	1.484805846787474E-4	0.0	6.0742057368578484E-5	2.6996469941590437E-5
20-21	4.0494704912385654E-5	1.6197881964954261E-4	0.0	6.74911748539761E-5	3.3745587426988044E-5
22-23	4.0494704912385654E-5	1.8897528959113306E-4	0.0	8.098940982477132E-5	4.7243822397783264E-5
24-25	4.0494704912385654E-5	2.024735245619283E-4	0.0	9.448764479556653E-5	5.3992939883180874E-5
26-27	4.0494704912385654E-5	2.159717595327235E-4	0.0	1.2823323222255458E-4	5.3992939883180874E-5
28-29	4.0494704912385654E-5	2.159717595327235E-4	0.0	3.9144881415306134E-4	5.3992939883180874E-5
30-31	4.0494704912385654E-5	2.2946999450351873E-4	0.0	0.001120353502576003	5.3992939883180874E-5
32-33	4.0494704912385654E-5	2.699646994159044E-4	0.0	0.002470176999655525	5.3992939883180874E-5
34-35	5.3992939883180874E-5	2.7671381690130197E-4	0.0	0.004508410480245603	5.3992939883180874E-5
36-37	5.3992939883180874E-5	2.834629343866996E-4	0.0	0.007633251875984697	5.3992939883180874E-5
38-39	5.3992939883180874E-5	2.834629343866996E-4	0.0	0.012404877938160806	5.3992939883180874E-5
40-41	5.3992939883180874E-5	3.2395763929908523E-4	0.0	0.01739247575986964	5.3992939883180874E-5
42-43	5.3992939883180874E-5	3.5770322672607326E-4	0.0	0.02275802416076074	5.3992939883180874E-5
44-45	5.3992939883180874E-5	4.656891064924351E-4	0.0	0.028218060206447404	5.3992939883180874E-5
46-47	5.3992939883180874E-5	5.399293988318088E-4	0.0	0.034609474465118945	8.098940982477131E-5
48-49	5.3992939883180874E-5	5.534276338026039E-4	0.0	0.04101438695876127	1.0798587976636175E-4
50-51	5.3992939883180874E-5	6.546643960835682E-4	0.0	0.04711558916556071	1.0798587976636175E-4
52-53	5.3992939883180874E-5	6.749117485397609E-4	0.0	0.05319654401990395	1.1473499725175935E-4
54-55	5.3992939883180874E-5	6.816608660251585E-4	0.0	0.05885230447266716	1.2148411473715697E-4
56-57	5.3992939883180874E-5	6.884099835105562E-4	0.0	0.06535170461110505	1.2148411473715697E-4
58-59	5.3992939883180874E-5	6.884099835105562E-4	0.0	0.07264075149533447	1.2148411473715697E-4
60-61	5.3992939883180874E-5	7.08657335966749E-4	0.0	0.07922114104359713	1.2148411473715697E-4
62-63	6.74911748539761E-5	7.491520408791346E-4	0.0	0.08543032913016294	1.2148411473715697E-4
64-65	6.74911748539761E-5	8.368905681893035E-4	0.0	0.09160577162930175	1.2148411473715697E-4
66-67	6.74911748539761E-5	8.368905681893035E-4	0.0	0.09776096677598436	1.2823323222255458E-4
68-69	6.74911748539761E-5	8.503888031600987E-4	0.0	0.10322775193915643	1.349823497079522E-4
70-71	6.74911748539761E-5	8.706361556162916E-4	0.0	0.10871478445478469	1.484805846787474E-4
72-73	6.74911748539761E-5	8.773852731016892E-4	0.0	0.11265626906625689	1.7547705462033785E-4
74-75	6.74911748539761E-5	9.043817430432797E-4	0.0	0.11559213517240485	1.7547705462033785E-4
76-77	6.74911748539761E-5	9.448764479556653E-4	0.0	0.11845376098621344	1.7547705462033785E-4
78-79	6.74911748539761E-5	9.651238004118581E-4	0.0	0.12042450329194954	1.7547705462033785E-4
80-81	6.74911748539761E-5	9.718729178972557E-4	0.0	0.1211129132754601	1.8222617210573544E-4
82-83	6.74911748539761E-5	9.786220353826534E-4	0.0	0.12156510414698174	1.8897528959113306E-4
84-85	7.42402923393737E-5	9.85371152868051E-4	0.0	0.12174058120160208	1.8897528959113306E-4
86-87	8.098940982477131E-5	9.988693878388462E-4	0.0	0.12188231266879543	1.8897528959113306E-4
88	8.098940982477131E-5	0.0011068552676052079	0.0	0.12190256002125162	1.8897528959113306E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10525	0.0	51.0357	1
GTATCAA	19485	0.0	37.4679	1
TCAACGC	24560	0.0	30.073128	4
ATCAACG	24775	0.0	29.829453	3
CAACGCA	25285	0.0	29.159779	5
TATCAAC	25660	0.0	29.102924	2
AACGCAG	26040	0.0	28.297861	6
TGGTATC	4980	0.0	24.963976	2
ACGCAGA	29910	0.0	24.636454	7
GTGGTAT	5180	0.0	24.248394	1
CGCAGAG	30985	0.0	23.753721	8
GCAGAGT	34890	0.0	21.156559	9
TACATGG	27635	0.0	20.352581	2
GTACATG	27855	0.0	20.130274	1
ACATGGG	27650	0.0	19.813326	3
GAGTACT	19675	0.0	19.085442	12-13
AGAGTAC	32425	0.0	18.719584	10-11
CAGAGTA	34645	0.0	18.577946	10-11
GTACTTT	21660	0.0	17.218048	14-15
CATGGGG	18295	0.0	17.151432	4
>>END_MODULE