FastQCFastQC Report
Fri 27 May 2016
SRR2139594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934101
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87400.051.395981
GTATCAA157500.036.6110881
TCAACGC195250.029.9708424
ATCAACG196150.029.8335783
TATCAAC202200.029.2389662
CAACGCA201250.029.055745
AACGCAG205950.028.3509966
GTGGTAT38100.025.6746061
CTTATAC45200.025.6282671
ACGCAGA234650.024.8833897
CGCAGAG240650.024.2984358
TGGTATC39750.024.06922
TATACAC62250.023.0879173
GCAGAGT276850.021.1522439
GAGTACT163000.020.87151112-13
CAGAGTA271200.019.29916210-11
GTACATG195800.019.0634081
TACATGG197650.019.036922
GTACTTT179700.018.82475314-15
ACATGGG196350.018.659773
Produced by FastQC (version 0.11.5)