##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139594_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5934101
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.449349446529475	18.0	18.0	30.0	18.0	33.0
2	29.837240046975946	30.0	27.0	33.0	27.0	33.0
3	31.090825720694678	33.0	31.0	33.0	28.0	33.0
4	32.43928591710859	33.0	33.0	33.0	31.0	34.0
5	32.947882754270616	33.0	33.0	34.0	33.0	34.0
6	36.99219359427823	38.0	37.0	38.0	35.0	38.0
7	37.051229158384736	38.0	38.0	38.0	36.0	38.0
8	36.99238300797374	38.0	38.0	38.0	35.0	38.0
9	37.26732355920467	38.0	38.0	38.0	36.0	38.0
10-11	37.31747622765437	38.0	38.0	38.0	37.0	38.0
12-13	37.352372751997315	38.0	38.0	38.0	37.0	38.0
14-15	37.369264695696955	38.0	38.0	38.0	37.0	38.0
16-17	37.385053270916686	38.0	38.0	38.0	37.0	38.0
18-19	37.42958065594098	38.0	38.0	38.0	37.0	38.0
20-21	37.44774959991746	38.0	38.0	38.0	37.0	38.0
22-23	37.47297949820609	38.0	38.0	38.0	37.0	38.0
24-25	37.48711611474133	38.0	38.0	38.0	37.0	38.0
26-27	37.462973859306246	38.0	38.0	38.0	37.0	38.0
28-29	37.438778137959844	38.0	38.0	38.0	37.0	38.0
30-31	37.445796897055104	38.0	38.0	38.0	37.0	38.0
32-33	37.433241320391446	38.0	38.0	38.0	37.0	38.0
34-35	37.403249087909316	38.0	38.0	38.0	37.0	38.0
36-37	37.38539708188365	38.0	38.0	38.0	37.0	38.0
38-39	37.36419663922625	38.0	38.0	38.0	37.0	38.0
40-41	37.354901961912915	38.0	38.0	38.0	37.0	38.0
42-43	37.332416080708384	38.0	38.0	38.0	37.0	38.0
44-45	37.29422864314664	38.0	38.0	38.0	37.0	38.0
46-47	37.246064628668755	38.0	38.0	38.0	37.0	38.0
48-49	37.18574547267812	38.0	38.0	38.0	37.0	38.0
50-51	37.09448274709143	38.0	38.0	38.0	36.0	38.0
52-53	37.10794287894514	38.0	38.0	38.0	36.0	38.0
54-55	37.08138434378756	38.0	38.0	38.0	36.0	38.0
56-57	37.02591837644708	38.0	38.0	38.0	36.0	38.0
58-59	37.00696030359764	38.0	38.0	38.0	36.0	38.0
60-61	37.021227214087816	38.0	38.0	38.0	36.0	38.0
62-63	37.01964377162191	38.0	38.0	38.0	36.0	38.0
64-65	36.96079465496091	38.0	38.0	38.0	36.0	38.0
66-67	36.99938589353188	38.0	38.0	38.0	36.0	38.0
68-69	37.00973736572763	38.0	38.0	38.0	36.0	38.0
70-71	36.99003413030664	38.0	38.0	38.0	36.0	38.0
72-73	36.981158018596744	38.0	38.0	38.0	36.0	38.0
74-75	36.93756359702854	38.0	38.0	38.0	36.0	38.0
76-77	36.940544857061	38.0	38.0	38.0	36.0	38.0
78-79	36.93942093664758	38.0	38.0	38.0	35.0	38.0
80-81	36.9359804761848	38.0	38.0	38.0	35.0	38.0
82-83	36.88057915207254	38.0	38.0	38.0	35.0	38.0
84-85	36.862347891190446	38.0	38.0	38.0	35.0	38.0
86-87	36.83119675456305	38.0	38.0	38.0	35.0	38.0
88-89	36.83617763726612	38.0	38.0	38.0	35.0	38.0
90-91	36.8201292887813	38.0	38.0	38.0	35.0	38.0
92-93	36.79752503335892	38.0	38.0	38.0	35.0	38.0
94-95	36.77947510628756	38.0	38.0	38.0	35.0	38.0
96-97	36.75952446131568	38.0	38.0	38.0	35.0	38.0
98-99	36.79175689558963	38.0	38.0	38.0	35.0	38.0
100	35.2081816342658	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	13.0
21	56.0
22	264.0
23	846.0
24	2005.0
25	4346.0
26	8275.0
27	14226.0
28	23333.0
29	34998.0
30	47156.0
31	61407.0
32	80131.0
33	109901.0
34	169277.0
35	318930.0
36	1065551.0
37	3993380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.014957615315275	19.456392804908443	17.696968757356842	22.831680822419436
2	14.811965503724558	20.74314889233952	41.749179033269755	22.695706570666164
3	18.51784257202397	25.384736199428858	30.773267435852585	25.324153792694588
4	12.895055961346092	17.701516793796394	37.76652753848269	31.63689970637482
5	13.735256612585461	36.8688534286828	35.32703605819989	14.068853900531858
6	30.72133420041216	37.05739083308491	18.277292550295318	13.943982416207612
7	27.10672266202769	32.2996739333573	22.3780401387717	18.21556326584332
8	24.503694830944063	36.78912778869116	20.685407949746725	18.02176943061805
9	25.498571729736312	17.029487701675453	21.187960905956942	36.28397966263129
10-11	24.52320916007328	26.64037905657487	28.329649259424468	20.50676252392738
12-13	25.26588273438555	24.139090318819985	28.68377198163631	21.911254965158157
14-15	22.349046637392924	25.547997919145633	26.439986444450476	25.66296899901097
16-17	21.883078835361918	28.73613037594069	27.178160600906526	22.202630187790874
18-19	21.864558759616664	27.65403723327257	29.145476290342888	21.33592771676788
20-21	22.650650346337116	26.722039935588466	29.1738473538348	21.453462364239616
22-23	22.40188205918604	26.734379743814728	29.033917798693853	21.829820398305376
24-25	22.21005173263324	26.862634770171145	29.125584592912997	21.80172890428262
26-27	22.09898388985968	26.962922421963743	29.111876620566978	21.826217067609598
28-29	21.987854041438972	26.838995484004663	29.23713688289926	21.9360135916571
30-31	22.203867153630068	26.82991821053438	29.17221453255665	21.7940001032789
32-33	21.848132970458767	26.90397996131765	29.27518008628048	21.97270698194311
34-35	22.061618196547943	26.895692616298543	29.14064984814803	21.902039339005476
36-37	22.04065237627676	26.854720036724856	29.179820031199128	21.924807555799255
38-39	21.91086141867686	26.95230899378929	29.25816278290654	21.878666804627308
40-41	22.165984702371215	26.803422102104246	29.13915267797859	21.891440517545945
42-43	22.147545107346062	26.85171079070532	28.944131346307778	22.056612755640842
44-45	22.084081561406563	27.05684949666951	28.844286852826766	22.01478208909716
46-47	22.317506792235463	26.973297400546887	28.42787332508277	22.281322482134875
48-49	22.220056775592564	27.041178449620617	28.42250142230919	22.31626335247763
50-51	22.138215812600016	27.25077014045872	28.375636019880595	22.23537802706067
52-53	22.33058773787998	27.19492896055592	28.37344358195785	22.10103971960625
54-55	22.298156108956153	27.153654815274226	28.255564341684597	22.292624734085027
56-57	22.343194781055818	27.37096597846092	28.075004100078356	22.210835140404907
58-59	22.151466560347913	27.38977199496027	28.331721863156556	22.127039581535264
60-61	22.289384170065397	27.55656437860144	28.09812373849544	22.055927712837725
62-63	22.188404415108582	27.711432879977743	28.111813115706724	21.988349589206948
64-65	22.162001762565332	27.593891403778887	28.133354902969554	22.110751930686227
66-67	22.220023557662604	27.531753540648733	28.06947413037043	22.178748771318226
68-69	22.199484613492224	27.4637431083179	28.17212402818765	22.164648250002227
70-71	22.175521880435245	27.153771213195277	28.299855124674128	22.37085178169535
72-73	22.322355872570785	27.060511193745402	28.289228457350173	22.32790447633364
74-75	22.191375993338795	27.318851818676432	28.183005849386177	22.306766338598592
76-77	22.32143106199927	27.21787313232103	28.114647942888226	22.346047862791472
78-79	22.234525012016427	27.187766308770478	28.10768285810581	22.47002582110729
80-81	22.27073725566778	27.316194613720413	27.995542812541373	22.417525318070435
82-83	22.39928970456534	27.2369866845267	28.078245406046236	22.285478204861725
84-85	22.407444565980025	27.253713565784548	28.01470540248161	22.324136465753813
86-87	22.310910653298258	27.326615250032365	28.024125623389573	22.338348473279808
88-89	22.409120449473946	27.284667291029923	27.94113593061266	22.365076328883468
90-91	22.42342833436143	27.34458322470552	27.941657202435056	22.290331238497995
92-93	22.438705348534235	27.35721204983533	27.88562007821017	22.318462523420266
94-95	22.325528622213834	27.384591718674738	27.938178352822835	22.35170130628859
96-97	22.46906094129383	27.499262808269982	27.91008443934256	22.121591811093637
98-99	23.49867601103442	29.418858818130477	28.157594256317125	18.924870914517975
100	22.481567597965377	30.239773780071104	22.04780891222052	25.230849709742998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1758.0
1	1566.5
2	1603.5
3	1946.5
4	2342.0
5	3089.5
6	4024.0
7	5900.0
8	7863.0
9	8598.5
10	8464.5
11	8190.0
12	8959.0
13	11120.5
14	15511.5
15	20958.5
16	24850.0
17	26346.0
18	25652.0
19	23423.0
20	20746.0
21	18684.5
22	18270.5
23	20346.5
24	24613.5
25	31286.5
26	40455.5
27	50997.0
28	61946.0
29	74428.0
30	87126.5
31	100030.0
32	115763.5
33	131299.0
34	145820.0
35	160683.5
36	176647.0
37	190968.5
38	201462.0
39	210352.0
40	218363.0
41	224605.0
42	228650.0
43	234241.5
44	242993.0
45	252958.0
46	254669.5
47	251137.5
48	250114.0
49	247377.0
50	242406.5
51	235143.0
52	226218.0
53	215247.5
54	201418.0
55	186649.0
56	171715.5
57	155445.5
58	138598.5
59	120978.5
60	102513.0
61	84136.0
62	66578.0
63	51504.5
64	39195.5
65	29247.5
66	21734.5
67	16495.5
68	12485.0
69	9137.5
70	6775.0
71	4962.0
72	3466.5
73	2378.0
74	1559.0
75	1009.0
76	637.5
77	360.0
78	236.5
79	161.5
80	100.0
81	69.5
82	54.5
83	32.0
84	19.5
85	15.0
86	8.5
87	5.0
88	4.5
89	4.0
90	2.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0040949758017263275
3	5.89811329466755E-4
4	8.425876135239357E-5
5	0.0
6	0.0
7	6.403665862781911E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.561410484927188E-4
24-25	6.320225074169948E-4
26-27	1.0113389639068294E-4
28-29	0.0025207194284997936
30-31	0.001881598197445802
32-33	0.004268366845929622
34-35	0.00924260377787829
36-37	0.001411166715235738
38-39	0.0
40-41	1.3746951613479713E-4
42-43	5.011926224100331E-4
44-45	1.8273308126610008E-4
46-47	8.774080243877316E-6
48-49	0.003954265688438541
50-51	0.0031908047780073332
52-53	3.320658588910364E-4
54-55	0.002711384752094025
56-57	0.04055609851971606
58-59	0.06231011801740166
60-61	0.02859872892070063
62-63	0.010234489221086331
64-65	7.148026025586547E-4
66-67	1.1388753093825957E-4
68-69	0.00131293511340501
70-71	0.010380362832413689
72-73	0.015879989158947633
74-75	0.009851832583998472
76-77	0.011793951695002034
78-79	0.0027557310909325887
80-81	0.013034161898310375
82-83	0.04309183948289793
84-85	0.023582169932474417
86-87	0.0036073576293574917
88-89	4.224853907192423E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	37.0
20-21	218.0
22-23	436.0
24-25	478.0
26-27	1395.0
28-29	3460.0
30-31	9677.0
32-33	12448.0
34-35	17774.0
36-37	28076.0
38-39	32686.0
40-41	32421.0
42-43	37590.0
44-45	46721.0
46-47	47441.0
48-49	43402.0
50-51	39328.0
52-53	38058.0
54-55	41582.0
56-57	43841.0
58-59	43658.0
60-61	43446.0
62-63	42681.0
64-65	46501.0
66-67	50447.0
68-69	51937.0
70-71	56532.0
72-73	45773.0
74-75	44957.0
76-77	47155.0
78-79	49752.0
80-81	49347.0
82-83	45729.0
84-85	45510.0
86-87	47392.0
88-89	50058.0
90-91	50312.0
92-93	49419.0
94-95	58111.0
96-97	182377.0
98-99	406507.0
100-101	3949431.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.65409252413693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.7085206584432	81.30335675705346
2	6.22144099677784	11.031124115236027
3	1.248797891445505	3.321331314364707
4	0.40453961835976543	1.4345637102298276
5	0.16740375637551003	0.7420514053301271
6	0.08783580093388701	0.4672201933754696
7	0.05176426345047734	0.3212379660967693
8	0.028168546583606503	0.19978055480748097
9	0.018213423020296122	0.145322504066032
>10	0.06126882624432623	0.8215035417727791
>50	0.0014384656681032044	0.08762991062708424
>100	5.53905890857514E-4	0.09561501370523225
>500	5.3846806577901026E-5	0.02926301333495526
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.6851752270478713E-5	0.0	0.0	0.0	0.0
3	1.6851752270478713E-5	1.6851752270478713E-5	0.0	0.0	0.0
4	1.6851752270478713E-5	5.055525681143614E-5	0.0	0.0	0.0
5	1.6851752270478713E-5	6.740700908191485E-5	0.0	0.0	0.0
6	6.740700908191485E-5	6.740700908191485E-5	0.0	0.0	0.0
7	6.740700908191485E-5	6.740700908191485E-5	0.0	0.0	0.0
8	6.740700908191485E-5	6.740700908191485E-5	0.0	0.0	0.0
9	6.740700908191485E-5	2.0222102724574455E-4	0.0	0.0	1.6851752270478713E-5
10-11	1.0111051362287227E-4	2.0222102724574455E-4	0.0	5.055525681143614E-5	1.6851752270478713E-5
12-13	1.2638814202859034E-4	2.0222102724574455E-4	0.0	6.740700908191485E-5	1.6851752270478713E-5
14-15	1.4323989429906907E-4	2.527762840571807E-4	0.0	6.740700908191485E-5	1.6851752270478713E-5
16-17	1.6009164656954778E-4	2.864797885981381E-4	0.0	8.425876135239357E-5	3.370350454095743E-5
18-19	1.7694339884002648E-4	2.9490566473337743E-4	0.0	1.1796226589335099E-4	6.740700908191485E-5
20-21	1.937951511105052E-4	3.033315408686168E-4	0.0	1.348140181638297E-4	6.740700908191485E-5
22-23	2.0222102724574455E-4	3.370350454095743E-4	0.0	1.4323989429906907E-4	6.740700908191485E-5
24-25	2.1907277951622328E-4	3.79164426085771E-4	0.0	1.516657704343084E-4	8.425876135239357E-5
26-27	2.527762840571807E-4	3.875903022210104E-4	0.0	1.7694339884002648E-4	8.425876135239357E-5
28-29	2.527762840571807E-4	3.875903022210104E-4	0.0	4.2129380676196777E-4	9.268463748763292E-5
30-31	2.864797885981381E-4	4.5499731130292523E-4	0.0	0.0011206415259868343	1.1796226589335099E-4
32-33	2.864797885981381E-4	4.7184906357340396E-4	0.0	0.0023760970701374984	1.516657704343084E-4
34-35	3.033315408686168E-4	5.729595771962763E-4	0.0	0.004170808686943481	1.516657704343084E-4
36-37	3.2018329313909555E-4	6.824959669543878E-4	0.0	0.007060884201330581	1.516657704343084E-4
38-39	3.2018329313909555E-4	7.077735953601059E-4	0.0	0.011450765667790287	1.516657704343084E-4
40-41	3.2018329313909555E-4	7.414770999010634E-4	0.0	0.016068145789901452	1.6851752270478714E-4
42-43	3.2018329313909555E-4	7.499029760363028E-4	0.0	0.020854043434717407	1.8536927497526584E-4
44-45	3.370350454095743E-4	8.762911180648931E-4	0.0	0.02606123488629533	2.106469033809839E-4
46-47	3.5388679768005296E-4	9.774016316877654E-4	0.0	0.03179083065825809	2.527762840571807E-4
48-49	3.5388679768005296E-4	9.774016316877654E-4	0.0	0.03722552076548748	2.696280363276594E-4
50-51	3.707385499505317E-4	0.0012638814202859034	0.0	0.043199466945372175	2.696280363276594E-4
52-53	3.707385499505317E-4	0.0012807331725563822	0.0	0.04891221096506447	2.7805391246289875E-4
54-55	3.79164426085771E-4	0.0012891590486916217	0.0	0.054153105921183345	2.864797885981381E-4
56-57	3.875903022210104E-4	0.0013312884293678183	0.0	0.0596467771613594	2.9490566473337743E-4
58-59	3.875903022210104E-4	0.0013312884293678183	0.0	0.06557859396056791	3.033315408686168E-4
60-61	3.875903022210104E-4	0.0013397143055030578	0.0	0.07134189323707163	3.033315408686168E-4
62-63	4.044420544914891E-4	0.0013565660577735364	0.0	0.07718102539879251	3.033315408686168E-4
64-65	4.212938067619678E-4	0.0014998059520726055	0.0	0.08206803355723133	3.033315408686168E-4
66-67	4.3814555903244656E-4	0.0015082318282078448	0.0	0.08762068593035408	3.117574170038562E-4
68-69	4.5499731130292523E-4	0.001516657704343084	0.0	0.09276889624898532	3.2018329313909555E-4
70-71	4.5499731130292523E-4	0.001516657704343084	0.0	0.09781599605399369	3.2018329313909555E-4
72-73	4.887008158438827E-4	0.001516657704343084	0.0	0.10191097185572001	4.2971968289720716E-4
74-75	4.887008158438827E-4	0.0015419353327488022	0.0	0.10464938159967281	4.3814555903244656E-4
76-77	4.887008158438827E-4	0.0015924905895602385	0.0	0.10744677247657228	4.465714351676859E-4
78-79	5.224043203848402E-4	0.0016009164656954778	0.0	0.10958694501492307	4.7184906357340396E-4
80-81	5.392560726553188E-4	0.0016009164656954778	0.0	0.11045481025685272	4.802749397086433E-4
82-83	5.476819487905582E-4	0.001609342341830717	0.0	0.11103619571018424	5.392560726553188E-4
84-85	5.561078249257975E-4	0.0016430458463716747	0.0	0.11127212024197095	5.392560726553188E-4
86-87	5.561078249257975E-4	0.001651471722506914	0.0	0.11133952725105285	5.645337010610368E-4
88	5.561078249257975E-4	0.0017862857406707436	0.0	0.11133952725105285	5.729595771962762E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8740	0.0	51.39598	1
GTATCAA	15750	0.0	36.611088	1
TCAACGC	19525	0.0	29.970842	4
ATCAACG	19615	0.0	29.833578	3
TATCAAC	20220	0.0	29.238966	2
CAACGCA	20125	0.0	29.05574	5
AACGCAG	20595	0.0	28.350996	6
GTGGTAT	3810	0.0	25.674606	1
CTTATAC	4520	0.0	25.628267	1
ACGCAGA	23465	0.0	24.883389	7
CGCAGAG	24065	0.0	24.298435	8
TGGTATC	3975	0.0	24.0692	2
TATACAC	6225	0.0	23.087917	3
GCAGAGT	27685	0.0	21.152243	9
GAGTACT	16300	0.0	20.871511	12-13
CAGAGTA	27120	0.0	19.299162	10-11
GTACATG	19580	0.0	19.063408	1
TACATGG	19765	0.0	19.03692	2
GTACTTT	17970	0.0	18.824753	14-15
ACATGGG	19635	0.0	18.65977	3
>>END_MODULE