FastQCFastQC Report
Fri 27 May 2016
SRR2139593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8268555
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121200.049.144491
GTATCAA219750.037.1394651
TCAACGC268250.031.1295174
ATCAACG270950.030.8353943
CAACGCA273400.030.5431355
TATCAAC283500.029.6235332
AACGCAG283450.029.429816
ACGCAGA327050.025.5064357
CGCAGAG337950.024.6965128
GCAGAGT385750.021.6920789
TGGTATC58750.021.5523572
GTGGTAT60400.021.4627251
GAGTACT217550.020.8450812-13
CAGAGTA372250.019.40716710-11
TACATGG281900.019.3569472
GTACATG278250.019.1619971
AGAGTAC344950.018.83941310-11
GTACTTT239000.018.83911114-15
ACATGGG280250.018.7324683
AGTACTT236900.017.35182612-13
Produced by FastQC (version 0.11.5)