##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139593_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8268555
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.617567398414838	18.0	18.0	30.0	18.0	33.0
2	29.92854567696532	30.0	28.0	33.0	27.0	33.0
3	31.178573886247356	33.0	31.0	33.0	29.0	33.0
4	32.462316087877504	33.0	33.0	33.0	31.0	34.0
5	32.963785691695826	33.0	33.0	34.0	33.0	34.0
6	37.01803761358544	38.0	38.0	38.0	35.0	38.0
7	37.085620280689916	38.0	38.0	38.0	36.0	38.0
8	37.030805261620685	38.0	38.0	38.0	36.0	38.0
9	37.29543275699321	38.0	38.0	38.0	36.0	38.0
10-11	37.33590778558043	38.0	38.0	38.0	37.0	38.0
12-13	37.36746638318303	38.0	38.0	38.0	37.0	38.0
14-15	37.383943083162656	38.0	38.0	38.0	37.0	38.0
16-17	37.398198778383886	38.0	38.0	38.0	37.0	38.0
18-19	37.43873808664271	38.0	38.0	38.0	37.0	38.0
20-21	37.45658523937677	38.0	38.0	38.0	37.0	38.0
22-23	37.48057560228713	38.0	38.0	38.0	37.0	38.0
24-25	37.49544375306054	38.0	38.0	38.0	37.0	38.0
26-27	37.471744480035866	38.0	38.0	38.0	37.0	38.0
28-29	37.44727529503618	38.0	38.0	38.0	37.0	38.0
30-31	37.4549569908987	38.0	38.0	38.0	37.0	38.0
32-33	37.44252051275465	38.0	38.0	38.0	37.0	38.0
34-35	37.41310893351924	38.0	38.0	38.0	37.0	38.0
36-37	37.395640160326394	38.0	38.0	38.0	37.0	38.0
38-39	37.38160114843201	38.0	38.0	38.0	37.0	38.0
40-41	37.365177864513626	38.0	38.0	38.0	37.0	38.0
42-43	37.34344607398033	38.0	38.0	38.0	37.0	38.0
44-45	37.30565093208216	38.0	38.0	38.0	37.0	38.0
46-47	37.257917368876505	38.0	38.0	38.0	37.0	38.0
48-49	37.199108440877424	38.0	38.0	38.0	37.0	38.0
50-51	37.110413826808056	38.0	38.0	38.0	36.0	38.0
52-53	37.12759441235791	38.0	38.0	38.0	36.0	38.0
54-55	37.1041296927995	38.0	38.0	38.0	36.0	38.0
56-57	37.049878316182976	38.0	38.0	38.0	36.0	38.0
58-59	37.035582952701965	38.0	38.0	38.0	36.0	38.0
60-61	37.056836430534034	38.0	38.0	38.0	36.0	38.0
62-63	37.053085862370224	38.0	38.0	38.0	36.0	38.0
64-65	36.99473273697898	38.0	38.0	38.0	36.0	38.0
66-67	37.03425096215502	38.0	38.0	38.0	36.0	38.0
68-69	37.04776039920302	38.0	38.0	38.0	36.0	38.0
70-71	37.026889629624335	38.0	38.0	38.0	36.0	38.0
72-73	37.02070491525818	38.0	38.0	38.0	36.0	38.0
74-75	36.97341365141281	38.0	38.0	38.0	36.0	38.0
76-77	36.97591909047227	38.0	38.0	38.0	36.0	38.0
78-79	36.97516808335744	38.0	38.0	38.0	36.0	38.0
80-81	36.970399101884425	38.0	38.0	38.0	36.0	38.0
82-83	36.91651124314487	38.0	38.0	38.0	35.0	38.0
84-85	36.896912763213926	38.0	38.0	38.0	35.0	38.0
86-87	36.865507772127835	38.0	38.0	38.0	35.0	38.0
88-89	36.87375085043428	38.0	38.0	38.0	35.0	38.0
90-91	36.857660669731686	38.0	38.0	38.0	35.0	38.0
92-93	36.835473739583335	38.0	38.0	38.0	35.0	38.0
94-95	36.817855268065735	38.0	38.0	38.0	35.0	38.0
96-97	36.79814743621218	38.0	38.0	38.0	35.0	38.0
98-99	36.83309041036104	38.0	38.0	38.0	35.0	38.0
100	35.296967978629034	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	15.0
21	97.0
22	348.0
23	1109.0
24	2847.0
25	6056.0
26	11099.0
27	19546.0
28	31379.0
29	47517.0
30	64375.0
31	84579.0
32	109203.0
33	148684.0
34	227613.0
35	427662.0
36	1416290.0
37	5670132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63647154793068	19.1294972337972	17.379167218455947	22.85486399981617
2	15.098080692596962	20.744395151424552	41.176535329210814	22.98098882676767
3	18.61476887598366	25.193484705719293	30.636260605482285	25.555485812814755
4	13.16013085728453	17.382564052947615	37.52644659583603	31.930858493931822
5	13.987208163941583	36.77929214959567	35.010555048614904	14.222944637847846
6	31.184215379833596	36.685224927451046	18.10018800141016	14.030371691305191
7	27.423620532724247	32.25470392638681	22.26927485675184	18.05240068413711
8	24.90507712653541	36.386587499266795	20.517659978073556	18.19067539612423
9	25.86732506465761	16.868896681463692	21.029006882097296	36.234771371781406
10-11	24.845145252102697	26.429593804479744	28.088245164965343	20.63701577845222
12-13	25.521768918511157	23.99219694372233	28.44890673158732	22.037127406179195
14-15	22.657671140846254	25.285204004810996	26.31069152953569	25.746433324807054
16-17	22.161550597413935	28.35647220100731	27.008431340179754	22.473545861399
18-19	22.17281012220394	27.41044233242689	28.929891619515143	21.486855925854034
20-21	23.018639458999196	26.51117890425338	28.90346423306366	21.56671740368377
22-23	22.781815519866676	26.31992620731774	28.78860419990086	22.109654072914722
24-25	22.488263955675	26.478990754013402	28.893372453682677	22.13937283662892
26-27	22.302582268779116	26.646500579918136	28.8658427006524	22.185074450650347
28-29	22.307580004618806	26.63619609226167	28.930637242311974	22.125586660807546
30-31	22.579762896692223	26.505210252941236	28.813931855308283	22.101094995058254
32-33	22.35419232984193	26.695801193213324	28.749059387518756	22.200947089425988
34-35	22.516275066105823	26.658076014387905	28.780056672292403	22.045592247213868
36-37	22.481197736351938	26.535701592515302	28.733309314230222	22.249791356902534
38-39	22.348779233365093	26.723662029209816	28.819391749782863	22.108166987642228
40-41	22.5990868930202	26.58943789240692	28.680883643893818	22.13059157067906
42-43	22.44750499184806	26.684384380407472	28.512007849822634	22.35610277792183
44-45	22.49118700590423	26.793763230703604	28.384076362338888	22.33097340105327
46-47	22.59304806690911	26.786160667484012	28.105597918852055	22.51519334675483
48-49	22.55290588551168	26.878741914529165	28.05008342006844	22.518268779890715
50-51	22.51201220152834	27.075485997748466	27.945809525186966	22.46669227553623
52-53	22.72892064452017	26.941665274454014	27.82265530279102	22.506758778234794
54-55	22.719319982763515	26.86532828669897	27.832181630283632	22.583170100253884
56-57	22.639115583958002	27.034375196217532	27.840977669997987	22.485531549826483
58-59	22.619249583269042	26.991673643605402	27.873774669328515	22.515302103797044
60-61	22.671556272862222	26.943852989422517	27.92071940896632	22.46387132874894
62-63	22.64230197309032	27.021964226237756	27.930399645702863	22.40533415496906
64-65	22.633919603313622	26.89311374828449	27.93275127417636	22.54021537422552
66-67	22.67573490716407	26.961510232802404	27.808740515342308	22.554014344691215
68-69	22.59427523922395	26.91113342531977	27.96978674638309	22.524804589073195
70-71	22.59794853807803	26.672081600357245	28.004661862834467	22.72530799873026
72-73	22.686394787682993	26.732866392435064	27.902378215461383	22.678360604420565
74-75	22.49596949845293	27.013590949265403	27.857012792552784	22.63342675972888
76-77	22.68570691607674	26.8792871036643	27.74652182132011	22.688484158938856
78-79	22.615005290686728	26.88920104245754	27.698437490748525	22.79735617610721
80-81	22.62534927771088	26.96949542254452	27.650329825659618	22.75482547408498
82-83	22.75382691476018	26.92163807861884	27.70362988656632	22.620905120054662
84-85	22.75559159998325	26.925349100811513	27.64187167547086	22.677187623734373
86-87	22.658954754428596	27.01619110072258	27.645442150722477	22.679411994126347
88-89	22.753736871824337	26.98337193245233	27.579888791695158	22.68300240402818
90-91	22.77616070345118	27.028045496500358	27.563742844379867	22.632050955668596
92-93	22.796191390421665	27.015623404910187	27.534003201543744	22.654182003124408
94-95	22.664636173464935	27.089509988615042	27.517513893798572	22.728339944121455
96-97	22.817814796298567	27.166228041215074	27.56025990523175	22.455697257254613
98-99	23.89977982422994	29.099670968703695	27.720576499138964	19.2799727079274
100	22.822690516210198	29.944829325677162	21.611605432981044	25.620874725131593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2371.0
1	2135.0
2	2247.0
3	2773.0
4	3361.5
5	4404.5
6	5632.0
7	8458.0
8	11501.5
9	12459.5
10	12151.0
11	11641.0
12	12359.0
13	15183.0
14	21227.5
15	28298.0
16	32949.0
17	34610.0
18	33623.0
19	30698.5
20	27092.0
21	23968.0
22	23105.0
23	25643.0
24	31287.0
25	40584.0
26	53032.0
27	67299.0
28	81276.5
29	96745.0
30	112407.5
31	128353.5
32	147779.0
33	167305.5
34	186831.0
35	206013.0
36	227631.0
37	246855.0
38	259996.5
39	273495.5
40	287102.0
41	297884.5
42	304835.5
43	314554.0
44	324883.0
45	332801.0
46	339347.5
47	342390.0
48	344585.0
49	346359.0
50	345311.5
51	340263.0
52	331650.5
53	317777.5
54	300888.0
55	282631.5
56	261437.0
57	238116.5
58	213949.0
59	187468.5
60	158660.5
61	129940.0
62	103145.0
63	80061.0
64	60623.5
65	45215.5
66	33640.5
67	25483.5
68	19386.5
69	14442.0
70	10628.5
71	7708.5
72	5467.0
73	3651.5
74	2339.0
75	1468.0
76	942.5
77	575.0
78	351.0
79	214.0
80	124.5
81	77.5
82	58.0
83	48.0
84	35.0
85	22.0
86	12.5
87	6.0
88	5.5
89	4.5
90	2.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0037733316159836877
3	8.707688344577741E-4
4	6.047005794845653E-5
5	0.0
6	0.0
7	4.4747842881857837E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.62404187189884E-4
24-25	4.838210834471624E-4
26-27	9.07255339091824E-5
28-29	0.0027104656949122805
30-31	0.0019314912759564574
32-33	0.00420255999578895
34-35	0.008963830972412185
36-37	0.0014021107252935898
38-39	0.0
40-41	1.3542265873459222E-4
42-43	6.188330961346076E-4
44-45	1.681866273566762E-4
46-47	3.76655495064683E-5
48-49	0.004099346913305645
50-51	0.003542385244716701
52-53	3.7196790840457E-4
54-55	0.002963481960885008
56-57	0.0400911898959795
58-59	0.0604917580143551
60-61	0.02850503256482022
62-63	0.009974425811764553
64-65	9.521197739855398E-4
66-67	1.2845410554532992E-4
68-69	0.0012412626532916752
70-71	0.011004397285582003
72-73	0.016149085917302793
74-75	0.009768897301578464
76-77	0.011927294659928311
78-79	0.002510988965130852
80-81	0.013882359145204
82-83	0.043272009979043485
84-85	0.023391572350647382
86-87	0.003455144732750487
88-89	1.3481773653153958E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	58.0
20-21	310.0
22-23	553.0
24-25	663.0
26-27	1794.0
28-29	4428.0
30-31	12187.0
32-33	16071.0
34-35	22407.0
36-37	35467.0
38-39	41696.0
40-41	41692.0
42-43	49638.0
44-45	60915.0
46-47	62187.0
48-49	57530.0
50-51	52140.0
52-53	51004.0
54-55	55730.0
56-57	58243.0
58-59	58751.0
60-61	57053.0
62-63	56269.0
64-65	60314.0
66-67	63793.0
68-69	65850.0
70-71	64799.0
72-73	61557.0
74-75	62845.0
76-77	66323.0
78-79	68416.0
80-81	68489.0
82-83	65530.0
84-85	63744.0
86-87	67196.0
88-89	69900.0
90-91	71637.0
92-93	71182.0
94-95	82684.0
96-97	260539.0
98-99	578402.0
100-101	5558569.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.2896299206125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.25592157963888	76.97894149671222
2	6.7560266914416784	11.524380324936816
3	1.6519945433004728	4.226940096869057
4	0.5809493285074172	1.9819581292410375
5	0.2867954636860226	1.2230339480345667
6	0.1511779625597246	0.7736347487322675
7	0.08644308613589102	0.5160889178008041
8	0.0595643604003135	0.40641778040005794
9	0.03444062991062374	0.26436857213788884
>10	0.1326188085274449	1.7431736371593114
>50	0.003106025700530368	0.1786392081183987
>100	8.900953607344729E-4	0.11705815615434387
>500	2.380828100185241E-5	0.01484368216732271
>1k	4.761654935843102E-5	0.050521301535844096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2094011589691307E-5
2	0.0	2.4188023179382614E-5	0.0	2.4188023179382614E-5	1.2094011589691307E-5
3	0.0	2.4188023179382614E-5	0.0	2.4188023179382614E-5	1.2094011589691307E-5
4	0.0	6.0470057948456535E-5	0.0	2.4188023179382614E-5	1.2094011589691307E-5
5	0.0	7.256406953814784E-5	0.0	4.837604635876523E-5	1.2094011589691307E-5
6	7.256406953814784E-5	7.256406953814784E-5	0.0	4.837604635876523E-5	1.2094011589691307E-5
7	7.256406953814784E-5	7.256406953814784E-5	0.0	6.0470057948456535E-5	1.2094011589691307E-5
8	7.256406953814784E-5	7.256406953814784E-5	0.0	7.256406953814784E-5	3.628203476907392E-5
9	8.465808112783915E-5	2.660682549732087E-4	0.0	7.256406953814784E-5	3.628203476907392E-5
10-11	1.0884610430722176E-4	2.660682549732087E-4	0.0	9.07050869226848E-5	3.628203476907392E-5
12-13	1.2094011589691307E-4	2.660682549732087E-4	0.0	9.675209271753046E-5	4.837604635876523E-5
14-15	1.3303412748660436E-4	3.688673534855848E-4	0.0	1.0279909851237612E-4	7.256406953814784E-5
16-17	2.1769220861444353E-4	3.870083708701218E-4	0.0	1.3303412748660436E-4	7.256406953814784E-5
18-19	2.2373921440928919E-4	4.2933741143404136E-4	0.0	1.7536316805052396E-4	8.465808112783915E-5
20-21	2.5397424338351746E-4	4.7166645199796096E-4	0.0	1.8745717964021525E-4	8.465808112783915E-5
22-23	2.9025627815259137E-4	5.321365099464175E-4	0.0	2.2373921440928919E-4	8.465808112783915E-5
24-25	2.9025627815259137E-4	5.623715389206457E-4	0.0	2.660682549732087E-4	8.465808112783915E-5
26-27	2.9025627815259137E-4	5.684185447154914E-4	0.0	3.1444430133197395E-4	9.07050869226848E-5
28-29	2.9025627815259137E-4	5.684185447154914E-4	0.0	4.777134577928066E-4	9.675209271753046E-5
30-31	2.9025627815259137E-4	7.014526722020957E-4	0.0	0.0010945080488670633	9.675209271753046E-5
32-33	2.9025627815259137E-4	7.195936895866327E-4	0.0	0.0024853193816815637	1.0884610430722176E-4
34-35	2.96303283947437E-4	8.284397938938545E-4	0.0	0.004118010946289889	1.0884610430722176E-4
36-37	3.1444430133197395E-4	9.372858982010762E-4	0.0	0.007002432710431266	1.0884610430722176E-4
38-39	3.507263361010479E-4	9.796149387649958E-4	0.0	0.011471169992822204	1.0884610430722176E-4
40-41	3.870083708701218E-4	0.0010521790083031436	0.0	0.016036659367930674	1.0884610430722176E-4
42-43	3.991023824598131E-4	0.0010884610430722174	0.0	0.020450973598168	1.1489311010206741E-4
44-45	4.2329040563919573E-4	0.001239636187943359	0.0	0.02556674050060742	1.2094011589691307E-4
46-47	4.2329040563919573E-4	0.0013545292980454264	0.0	0.03088205859427675	1.2094011589691307E-4
48-49	4.2933741143404136E-4	0.0013666233096351176	0.0	0.035979684479331635	1.3303412748660436E-4
50-51	4.474784288185783E-4	0.0015419864776856414	0.0	0.041512694781615414	1.3303412748660436E-4
52-53	4.474784288185783E-4	0.0015722215066598698	0.0	0.04687034191584866	1.5117514487114132E-4
54-55	4.474784288185783E-4	0.001584315518249561	0.0	0.05257266838038811	1.7536316805052396E-4
56-57	4.474784288185783E-4	0.0016085035414289438	0.0	0.05857734513466985	1.814101738453696E-4
58-59	4.474784288185783E-4	0.0016266445588134807	0.0	0.06465458595848972	1.935041854350609E-4
60-61	4.474784288185783E-4	0.001693161622556783	0.0	0.07096161300251375	2.055981970247522E-4
62-63	4.53525434613424E-4	0.0017294436573258567	0.0	0.07717793495961507	2.2978622020413482E-4
64-65	4.6561944620311527E-4	0.0019108538311712264	0.0	0.08307376560958958	2.2978622020413482E-4
66-67	4.837604635876523E-4	0.0019108538311712264	0.0	0.08914495942761462	2.2978622020413482E-4
68-69	4.837604635876523E-4	0.0019592298775299916	0.0	0.09476262781102623	2.479272375886718E-4
70-71	4.837604635876523E-4	0.001971323889119683	0.0	0.09922531808762233	2.5397424338351746E-4
72-73	5.321365099464175E-4	0.0019955119122990654	0.0	0.10293213263986271	2.660682549732087E-4
74-75	5.321365099464175E-4	0.002092264005016596	0.0	0.10591330649672162	2.660682549732087E-4
76-77	5.381835157412631E-4	0.002164828074554744	0.0	0.10867678814496609	2.660682549732087E-4
78-79	5.442305215361088E-4	0.0021890160977341266	0.0	0.11067834706305998	2.660682549732087E-4
80-81	5.442305215361088E-4	0.0021890160977341266	0.0	0.11132537668310848	2.7211526076805436E-4
82-83	5.442305215361088E-4	0.0022071571151186633	0.0	0.11174262008295283	2.7816226656290005E-4
84-85	5.623715389206457E-4	0.002213204120913509	0.0	0.11194821827997758	2.8420927235774573E-4
86-87	5.684185447154914E-4	0.002213204120913509	0.0	0.1120570643842848	2.9025627815259137E-4
88	5.684185447154914E-4	0.0023462382484001135	0.0	0.11206311139007964	2.9025627815259137E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12120	0.0	49.14449	1
GTATCAA	21975	0.0	37.139465	1
TCAACGC	26825	0.0	31.129517	4
ATCAACG	27095	0.0	30.835394	3
CAACGCA	27340	0.0	30.543135	5
TATCAAC	28350	0.0	29.623533	2
AACGCAG	28345	0.0	29.42981	6
ACGCAGA	32705	0.0	25.506435	7
CGCAGAG	33795	0.0	24.696512	8
GCAGAGT	38575	0.0	21.692078	9
TGGTATC	5875	0.0	21.552357	2
GTGGTAT	6040	0.0	21.462725	1
GAGTACT	21755	0.0	20.84508	12-13
CAGAGTA	37225	0.0	19.407167	10-11
TACATGG	28190	0.0	19.356947	2
GTACATG	27825	0.0	19.161997	1
AGAGTAC	34495	0.0	18.839413	10-11
GTACTTT	23900	0.0	18.839111	14-15
ACATGGG	28025	0.0	18.732468	3
AGTACTT	23690	0.0	17.351826	12-13
>>END_MODULE