FastQCFastQC Report
Fri 27 May 2016
SRR2139592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5303104
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85450.053.0316351
GTATCAA159600.037.0369261
TCAACGC202000.029.6851524
ATCAACG203250.029.5025883
TATCAAC206200.029.30892
CAACGCA207000.028.9681195
TGGTATC38050.028.8476332
AACGCAG214650.027.876026
GTGGTAT39700.027.1107641
ACGCAGA245700.024.3358447
CGCAGAG250900.023.8484958
GCAGAGT281900.021.3016769
TACATGG228900.020.263562
GTACATG232200.020.0859411
GAGTACT151150.020.04784812-13
ACATGGG230300.019.5465553
CAGAGTA280100.018.998910-11
AGAGTAC264700.018.32938610-11
GTACTTT166200.018.15946214-15
CATGGGG133150.017.5135844
Produced by FastQC (version 0.11.5)