##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139592_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5303104
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.884552518675854	18.0	18.0	30.0	18.0	33.0
2	30.082011591701765	31.0	29.0	33.0	27.0	33.0
3	31.297736382314962	33.0	31.0	33.0	29.0	33.0
4	32.50926099129868	33.0	33.0	33.0	31.0	34.0
5	33.00370424566442	33.0	33.0	34.0	33.0	34.0
6	37.06286884058846	38.0	38.0	38.0	36.0	38.0
7	37.148590712156505	38.0	38.0	38.0	36.0	38.0
8	37.09493176071976	38.0	38.0	38.0	36.0	38.0
9	37.34070763085167	38.0	38.0	38.0	37.0	38.0
10-11	37.37832541092915	38.0	38.0	38.0	37.0	38.0
12-13	37.402262618270356	38.0	38.0	38.0	37.0	38.0
14-15	37.420965061216975	38.0	38.0	38.0	37.0	38.0
16-17	37.43986729281568	38.0	38.0	38.0	37.0	38.0
18-19	37.47490045452626	38.0	38.0	38.0	37.0	38.0
20-21	37.48889011631388	38.0	38.0	38.0	37.0	38.0
22-23	37.511021072016	38.0	38.0	38.0	37.0	38.0
24-25	37.52524076053015	38.0	38.0	38.0	37.5	38.0
26-27	37.50270769465597	38.0	38.0	38.0	37.0	38.0
28-29	37.47835596771322	38.0	38.0	38.0	37.0	38.0
30-31	37.48608022205565	38.0	38.0	38.0	37.0	38.0
32-33	37.47409252399493	38.0	38.0	38.0	37.0	38.0
34-35	37.44233271170964	38.0	38.0	38.0	37.0	38.0
36-37	37.42674326722697	38.0	38.0	38.0	37.0	38.0
38-39	37.414938279334244	38.0	38.0	38.0	37.0	38.0
40-41	37.39805039942604	38.0	38.0	38.0	37.0	38.0
42-43	37.37403629873005	38.0	38.0	38.0	37.0	38.0
44-45	37.33601437135319	38.0	38.0	38.0	37.0	38.0
46-47	37.28579271070191	38.0	38.0	38.0	37.0	38.0
48-49	37.228693133792646	38.0	38.0	38.0	37.0	38.0
50-51	37.14562097983671	38.0	38.0	38.0	37.0	38.0
52-53	37.16890420270933	38.0	38.0	38.0	37.0	38.0
54-55	37.149839035341145	38.0	38.0	38.0	36.5	38.0
56-57	37.102178150226365	38.0	38.0	38.0	36.0	38.0
58-59	37.091975106566004	38.0	38.0	38.0	36.0	38.0
60-61	37.110886466132314	38.0	38.0	38.0	36.0	38.0
62-63	37.115009675142716	38.0	38.0	38.0	36.0	38.0
64-65	37.05993770447567	38.0	38.0	38.0	36.0	38.0
66-67	37.10616671651926	38.0	38.0	38.0	36.0	38.0
68-69	37.122108402205754	38.0	38.0	38.0	36.0	38.0
70-71	37.10929512569566	38.0	38.0	38.0	36.0	38.0
72-73	37.10591961282101	38.0	38.0	38.0	36.0	38.0
74-75	37.066656545050904	38.0	38.0	38.0	36.0	38.0
76-77	37.070687024600154	38.0	38.0	38.0	36.0	38.0
78-79	37.0698974651947	38.0	38.0	38.0	36.0	38.0
80-81	37.06793166026076	38.0	38.0	38.0	36.0	38.0
82-83	37.017153259499615	38.0	38.0	38.0	36.0	38.0
84-85	37.00150447957378	38.0	38.0	38.0	36.0	38.0
86-87	36.965874078475316	38.0	38.0	38.0	36.0	38.0
88-89	36.97181924798223	38.0	38.0	38.0	36.0	38.0
90-91	36.957302698454896	38.0	38.0	38.0	35.5	38.0
92-93	36.93599894587703	38.0	38.0	38.0	35.0	38.0
94-95	36.91725713113887	38.0	38.0	38.0	35.0	38.0
96-97	36.89290846070716	38.0	38.0	38.0	35.0	38.0
98-99	36.91993816404475	38.0	38.0	38.0	35.0	38.0
100	35.392162605486305	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	53.0
22	198.0
23	619.0
24	1697.0
25	3534.0
26	6660.0
27	11393.0
28	18863.0
29	27570.0
30	38763.0
31	50908.0
32	65756.0
33	90039.0
34	135884.0
35	257635.0
36	863064.0
37	3730455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90259214226234	19.455360483218882	17.164268322853935	22.477779051664836
2	14.789049785872285	20.958832037503935	41.91772064771983	22.334397528903942
3	18.531065413455003	25.73563077071612	30.959908490707644	24.773395325121232
4	12.769224502941864	17.652460767301857	38.36418760189791	31.214127127858376
5	13.433000748241032	37.36134535547483	35.343036832768135	13.862617063516009
6	30.214776100940128	37.32510243057651	18.601992342597846	13.858129125885519
7	26.881689407280728	32.70524396542604	22.670126551295695	17.74294007599753
8	24.551262807593442	36.62977380794342	20.876245308408055	17.94271807605508
9	25.763552817368847	16.956691779003393	21.43225929568796	35.847496107939804
10-11	24.38164516479405	26.82084869540556	28.507747538045642	20.289758601754745
12-13	25.24233920360604	24.498058118415177	28.857863243866234	21.40173943411255
14-15	22.367475727423034	25.740830276004395	26.93606423709586	24.955629759476714
16-17	21.496212406922435	28.88073664027709	27.692583437926167	21.93046751487431
18-19	21.51141105284754	27.764861862034007	29.784066086578733	20.939660998539722
20-21	22.59010359388376	26.787931379495888	29.746563043235124	20.875401983385224
22-23	22.246258805619185	26.65949813688968	29.61936521725597	21.474877840235166
24-25	21.8436283701868	26.843768463958117	29.78246409000416	21.53013907585092
26-27	21.705010670446764	27.016248311114126	29.717305108538312	21.5614359099008
28-29	21.615584597511774	27.00159029394904	29.826106024568137	21.55671908397105
30-31	21.9400208197006	26.9115226971206	29.67102114225668	21.477435340922124
32-33	21.700919176607197	27.09217707558153	29.58283421289262	21.62406953491865
34-35	21.88089742896524	27.038533742756126	29.637476794473738	21.443092033804888
36-37	21.84150664278276	26.909803612545485	29.586797765480522	21.661891979191232
38-39	21.64364319639622	27.115252641017477	29.67307845321451	21.56802570937179
40-41	21.930538516320773	26.973175441731012	29.539208873911672	21.55707716803655
42-43	21.857285728060234	27.058850950043244	29.284362876877307	21.799500445019213
44-45	21.911324988304706	27.179451815831207	29.156036685087912	21.753186510776178
46-47	21.951890078920027	27.209620255452332	28.861640561353134	21.976849104274507
48-49	21.945464105679168	27.301479089944426	28.78349131478448	21.96956548959193
50-51	21.974969332122217	27.54071418257856	28.455830856504733	22.028485628794485
52-53	22.162422459300778	27.513989049232855	28.239642293500005	22.083946197966355
54-55	22.196513409494546	27.477444250722392	28.247052512222776	22.07898982756028
56-57	22.107317355050814	27.592393144108634	28.212428618617068	22.087860882223485
58-59	22.077931740902134	27.524580500778356	28.2968315066919	22.10065625162761
60-61	22.128304230112626	27.52100365346616	28.324703693110926	22.02598842331029
62-63	22.0897436247615	27.541612310112747	28.330345254901335	22.03829881022442
64-65	22.07921620725419	27.41881488214611	28.376721406301147	22.12524750429855
66-67	22.15043438744687	27.42268438076399	28.313903071453396	22.112978160335746
68-69	22.14638540838137	27.399880308425516	28.33239708451795	22.121337198675164
70-71	22.08381100311199	27.1948467293573	28.393273391457246	22.328068876073463
72-73	22.19244697794735	27.32131420089675	28.292877508444374	22.193361312711524
74-75	22.054029770916394	27.533644204579005	28.172874361795643	22.239451662708962
76-77	22.180471856122008	27.45666080294934	28.08847119806806	22.274396142860592
78-79	22.182376764352902	27.484345387161124	27.98623930399081	22.34703854449516
80-81	22.164227236735563	27.57356205718387	27.93452625284886	22.32768445323171
82-83	22.312872458581882	27.538019412070053	27.964667364580947	22.18444076476712
84-85	22.326668208575782	27.546288322475526	27.879579710257	22.247463758691694
86-87	22.182272501984666	27.607852410024964	27.958949976023067	22.250925111967305
88-89	22.294036494789783	27.58897185775701	27.8863891880264	22.230602459426805
90-91	22.330902464899864	27.634430511048958	27.8436312009754	22.19103582307578
92-93	22.347515231816637	27.656061807316174	27.806334201296163	22.19008875957103
94-95	22.22716378789814	27.69896483360342	27.804291681596755	22.269579696901694
96-97	22.32976659701507	27.82007714672517	27.85706564778632	21.99309060847344
98-99	23.343910358217105	29.683048534826202	28.14737938285809	18.825661724098605
100	22.292694129153958	30.58481399545821	22.011339823059213	25.111152052328624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2251.0
1	1857.5
2	1510.5
3	1749.5
4	2333.0
5	3143.5
6	3740.5
7	3966.5
8	3951.0
9	4056.5
10	4537.5
11	5780.0
12	7594.0
13	11930.5
14	18867.5
15	25126.5
16	29459.5
17	30832.0
18	30090.0
19	27102.5
20	23281.5
21	20487.5
22	19384.0
23	21105.0
24	25306.0
25	32019.0
26	41301.0
27	51945.5
28	61983.0
29	73257.0
30	84821.5
31	96000.5
32	109682.5
33	123843.0
34	137732.0
35	151188.5
36	165529.5
37	177546.5
38	185242.0
39	192735.5
40	200150.5
41	205936.0
42	208083.5
43	211743.5
44	217020.5
45	219954.0
46	220736.5
47	219658.5
48	219209.5
49	217647.5
50	213585.5
51	207410.5
52	198782.5
53	187427.0
54	174979.0
55	162039.0
56	147113.5
57	131577.5
58	116641.5
59	101871.5
60	85888.5
61	69271.0
62	53992.0
63	41481.5
64	31598.5
65	23328.5
66	16740.0
67	12422.0
68	9449.5
69	6853.0
70	4966.5
71	3675.0
72	2638.5
73	1813.5
74	1241.0
75	844.5
76	536.0
77	337.5
78	223.5
79	137.0
80	90.0
81	64.5
82	52.0
83	39.0
84	21.0
85	11.0
86	7.0
87	3.5
88	1.5
89	0.5
90	0.5
91	1.0
92	1.0
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0036582348752730474
3	6.788477088135553E-4
4	3.771376160075307E-5
5	0.0
6	0.0
7	6.977045896139317E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.942298582959864E-4
24-25	4.339586205361106E-4
26-27	7.548614730121455E-5
28-29	0.002652464810004229
30-31	0.001889572263195497
32-33	0.0042871176373723095
34-35	0.009105887240660063
36-37	0.0015226995483482802
38-39	0.0
40-41	2.0193816403398602E-4
42-43	6.576357164278659E-4
44-45	1.7531951250656353E-4
46-47	2.946755371861374E-5
48-49	0.004001878109132405
50-51	0.0032096748599366883
52-53	3.12693636795447E-4
54-55	0.002712712471680349
56-57	0.038580440525746836
58-59	0.05948854721531632
60-61	0.026811637000434048
62-63	0.009600989595216396
64-65	0.001059397224167393
66-67	9.623343408261042E-5
68-69	0.001263191170703984
70-71	0.010295882279674118
72-73	0.01500165216433528
74-75	0.009061777976432706
76-77	0.010981332520699756
78-79	0.0024734964290313594
80-81	0.012950540292182082
82-83	0.041625529087722615
84-85	0.022162959603203365
86-87	0.0031952443403347056
88-89	8.374912781265464E-5
90-91	1.210651994210178E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	435.0
20-21	1476.0
22-23	907.0
24-25	1001.0
26-27	1629.0
28-29	3369.0
30-31	8587.0
32-33	10961.0
34-35	15296.0
36-37	24053.0
38-39	28650.0
40-41	28846.0
42-43	34120.0
44-45	42223.0
46-47	43555.0
48-49	40137.0
50-51	52388.0
52-53	34788.0
54-55	38532.0
56-57	40533.0
58-59	41046.0
60-61	40782.0
62-63	39749.0
64-65	42746.0
66-67	44749.0
68-69	46410.0
70-71	45828.0
72-73	42749.0
74-75	43312.0
76-77	45688.0
78-79	47606.0
80-81	47404.0
82-83	44425.0
84-85	43082.0
86-87	45162.0
88-89	48134.0
90-91	49220.0
92-93	48498.0
94-95	53030.0
96-97	159568.0
98-99	353187.0
100-101	3479243.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.18508856314274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.3016476130688	82.31930616895534
2	5.759691281821126	10.273571541311565
3	1.1726028787932345	3.137360747837123
4	0.3757440876545054	1.3404307893817735
5	0.16592621896276905	0.7399072266570986
6	0.07432022343225317	0.3976953425302834
7	0.04096760053890877	0.25575893575974235
8	0.02487522989924779	0.17747996652743603
9	0.021028076556699735	0.16878517830196305
>10	0.060552948878294106	0.8670301370366746
>50	0.0016096994099790306	0.09789860300887551
>100	9.147262479436219E-4	0.14479375909403647
>500	8.956105279036264E-5	0.0516259423567471
>1k	2.9853683455854123E-5	0.028355661241378942
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.8856880800376534E-5	0.0
3	0.0	0.0	0.0	1.8856880800376534E-5	0.0
4	0.0	3.771376160075307E-5	0.0	1.8856880800376534E-5	0.0
5	0.0	3.771376160075307E-5	0.0	1.8856880800376534E-5	0.0
6	0.0	3.771376160075307E-5	0.0	1.8856880800376534E-5	0.0
7	0.0	3.771376160075307E-5	0.0	1.8856880800376534E-5	0.0
8	0.0	3.771376160075307E-5	0.0	5.65706424011296E-5	0.0
9	0.0	9.428440400188267E-5	0.0	5.65706424011296E-5	0.0
10-11	0.0	9.428440400188267E-5	0.0	5.65706424011296E-5	0.0
12-13	0.0	9.428440400188267E-5	0.0	5.65706424011296E-5	0.0
14-15	0.0	1.5085504640301227E-4	0.0	5.65706424011296E-5	1.8856880800376534E-5
16-17	0.0	1.5085504640301227E-4	0.0	5.65706424011296E-5	1.8856880800376534E-5
18-19	0.0	1.6028348680320056E-4	0.0	8.48559636016944E-5	2.82853212005648E-5
20-21	0.0	1.8856880800376534E-4	0.0	1.5085504640301227E-4	3.771376160075307E-5
22-23	0.0	1.8856880800376534E-4	0.0	1.6971192720338882E-4	4.7142202000941334E-5
24-25	0.0	2.0742568880414188E-4	0.0	1.7914036760357708E-4	5.65706424011296E-5
26-27	0.0	2.262825696045184E-4	0.0	2.7342477160545973E-4	5.65706424011296E-5
28-29	0.0	2.451394504048949E-4	0.0	4.619935796092251E-4	7.542752320150613E-5
30-31	0.0	3.0171009280602454E-4	0.0	0.001272839454025416	9.428440400188267E-5
32-33	0.0	3.2056697360640106E-4	0.0	0.0032056697360640105	1.2256972520244746E-4
34-35	0.0	3.8656605640771896E-4	0.0	0.0061096293793219975	1.5085504640301227E-4
36-37	0.0	4.525651392090368E-4	0.0	0.010559853248210859	1.5085504640301227E-4
38-39	0.0	4.525651392090368E-4	0.0	0.016678911067933046	1.5085504640301227E-4
40-41	0.0	4.902789008097898E-4	0.0	0.023278819348064833	1.5085504640301227E-4
42-43	0.0	4.997073412099781E-4	0.0	0.02948273313138871	1.5085504640301227E-4
44-45	9.428440400188267E-6	5.468495432109195E-4	0.0	0.03718576893834252	1.5085504640301227E-4
46-47	1.8856880800376534E-5	5.845633048116726E-4	0.0	0.045633651536911214	1.5085504640301227E-4
48-49	1.8856880800376534E-5	5.939917452118608E-4	0.0	0.05412867633748084	1.6971192720338882E-4
50-51	1.8856880800376534E-5	6.222770664124257E-4	0.0	0.06319883600246196	1.6971192720338882E-4
52-53	1.8856880800376534E-5	6.222770664124257E-4	0.0	0.07221242502504194	1.8856880800376536E-4
54-55	1.8856880800376534E-5	6.411339472128022E-4	0.0	0.07957603697758897	2.0742568880414188E-4
56-57	1.8856880800376534E-5	6.599908280131787E-4	0.0	0.08602509021131774	2.1685412920433014E-4
58-59	1.8856880800376534E-5	6.788477088135553E-4	0.0	0.09215357647144012	2.262825696045184E-4
60-61	1.8856880800376534E-5	6.788477088135553E-4	0.0	0.0984329177779655	2.262825696045184E-4
62-63	1.8856880800376534E-5	6.788477088135553E-4	0.0	0.10367513064047018	2.262825696045184E-4
64-65	1.8856880800376534E-5	7.731321128154379E-4	0.0	0.10890791506257466	2.262825696045184E-4
66-67	1.8856880800376534E-5	7.731321128154379E-4	0.0	0.11502697288229685	2.262825696045184E-4
68-69	1.8856880800376534E-5	7.825605532156262E-4	0.0	0.12070289400321019	2.3571101000470666E-4
70-71	1.8856880800376534E-5	8.014174340160026E-4	0.0	0.12571882429611037	2.451394504048949E-4
72-73	1.8856880800376534E-5	8.10845874416191E-4	0.0	0.12990505183379394	2.545678908050832E-4
74-75	1.8856880800376534E-5	8.391311956167558E-4	0.0	0.1332992903778617	2.639963312052715E-4
76-77	1.8856880800376534E-5	8.768449572175089E-4	0.0	0.13682552708753212	2.639963312052715E-4
78-79	1.8856880800376534E-5	9.051302784180736E-4	0.0	0.13928635003198125	2.639963312052715E-4
80-81	1.8856880800376534E-5	9.051302784180736E-4	0.0	0.1399840546215952	2.82853212005648E-4
82-83	1.8856880800376534E-5	9.051302784180736E-4	0.0	0.14047433352240501	3.0171009280602454E-4
84-85	1.8856880800376534E-5	9.051302784180736E-4	0.0	0.1407100445324097	3.0171009280602454E-4
86-87	1.8856880800376534E-5	9.051302784180736E-4	0.0	0.1408137573768118	3.0171009280602454E-4
88	1.8856880800376534E-5	9.428440400188267E-4	0.0	0.14082318581721195	3.0171009280602454E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8545	0.0	53.031635	1
GTATCAA	15960	0.0	37.036926	1
TCAACGC	20200	0.0	29.685152	4
ATCAACG	20325	0.0	29.502588	3
TATCAAC	20620	0.0	29.3089	2
CAACGCA	20700	0.0	28.968119	5
TGGTATC	3805	0.0	28.847633	2
AACGCAG	21465	0.0	27.87602	6
GTGGTAT	3970	0.0	27.110764	1
ACGCAGA	24570	0.0	24.335844	7
CGCAGAG	25090	0.0	23.848495	8
GCAGAGT	28190	0.0	21.301676	9
TACATGG	22890	0.0	20.26356	2
GTACATG	23220	0.0	20.085941	1
GAGTACT	15115	0.0	20.047848	12-13
ACATGGG	23030	0.0	19.546555	3
CAGAGTA	28010	0.0	18.9989	10-11
AGAGTAC	26470	0.0	18.329386	10-11
GTACTTT	16620	0.0	18.159462	14-15
CATGGGG	13315	0.0	17.513584	4
>>END_MODULE