FastQCFastQC Report
Fri 27 May 2016
SRR2139591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6454384
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94900.052.017661
GTATCAA177600.037.9904371
TCAACGC224750.030.389454
ATCAACG227000.030.0305293
CAACGCA229250.029.7738065
TATCAAC231850.029.6116962
AACGCAG236050.028.8975266
ACGCAGA270050.025.2430177
GTGGTAT49550.025.216211
TGGTATC46600.025.1190952
CGCAGAG275200.024.8024888
GCAGAGT316950.021.6045559
GTACATG219450.020.6168691
GAGTACT187700.020.61130712-13
TACATGG224300.020.5815322
ACATGGG225300.020.1584433
AGAGTAC289950.019.34515610-11
CAGAGTA308650.019.18153610-11
GTACTTT202450.018.99088914-15
CATGGGG151950.018.1758584
Produced by FastQC (version 0.11.5)