##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139591_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6454384
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.644480247843948	18.0	18.0	30.0	18.0	33.0
2	30.364227012213714	31.0	29.0	33.0	27.0	33.0
3	31.479798846799323	33.0	31.0	33.0	29.0	33.0
4	32.54900018344121	33.0	33.0	33.0	31.0	34.0
5	33.02632226406114	33.0	33.0	34.0	33.0	34.0
6	37.06865349195213	38.0	38.0	38.0	36.0	38.0
7	37.16659436438861	38.0	38.0	38.0	36.0	38.0
8	37.105501470008605	38.0	38.0	38.0	36.0	38.0
9	37.337223815626714	38.0	38.0	38.0	37.0	38.0
10-11	37.37146945394014	38.0	38.0	38.0	37.0	38.0
12-13	37.39225656546	38.0	38.0	38.0	37.0	38.0
14-15	37.41571829317871	38.0	38.0	38.0	37.0	38.0
16-17	37.43302815574654	38.0	38.0	38.0	37.0	38.0
18-19	37.4564572699734	38.0	38.0	38.0	37.0	38.0
20-21	37.47421601603071	38.0	38.0	38.0	37.0	38.0
22-23	37.490567298561395	38.0	38.0	38.0	37.0	38.0
24-25	37.50503707883854	38.0	38.0	38.0	37.0	38.0
26-27	37.47986559441232	38.0	38.0	38.0	37.0	38.0
28-29	37.452956291262424	38.0	38.0	38.0	37.0	38.0
30-31	37.4612049018491	38.0	38.0	38.0	37.0	38.0
32-33	37.449015146555624	38.0	38.0	38.0	37.0	38.0
34-35	37.415997259605625	38.0	38.0	38.0	37.0	38.0
36-37	37.39691184870862	38.0	38.0	38.0	37.0	38.0
38-39	37.38468774122224	38.0	38.0	38.0	37.0	38.0
40-41	37.370646437877085	38.0	38.0	38.0	37.0	38.0
42-43	37.34424948307283	38.0	38.0	38.0	37.0	38.0
44-45	37.30876508987349	38.0	38.0	38.0	37.0	38.0
46-47	37.26358334887731	38.0	38.0	38.0	37.0	38.0
48-49	37.20316728871981	38.0	38.0	38.0	37.0	38.0
50-51	37.12002333767642	38.0	38.0	38.0	37.0	38.0
52-53	37.14019426914594	38.0	38.0	38.0	37.0	38.0
54-55	37.124171667666616	38.0	38.0	38.0	36.5	38.0
56-57	37.07607587062465	38.0	38.0	38.0	36.0	38.0
58-59	37.063702691084856	38.0	38.0	38.0	36.0	38.0
60-61	37.08488397120914	38.0	38.0	38.0	36.0	38.0
62-63	37.087706845689894	38.0	38.0	38.0	36.0	38.0
64-65	37.032549373344466	38.0	38.0	38.0	36.0	38.0
66-67	37.07578892987045	38.0	38.0	38.0	36.0	38.0
68-69	37.09076554034429	38.0	38.0	38.0	36.0	38.0
70-71	37.0733092209217	38.0	38.0	38.0	36.0	38.0
72-73	37.0702304031039	38.0	38.0	38.0	36.0	38.0
74-75	37.02784715435645	38.0	38.0	38.0	36.0	38.0
76-77	37.033624725905256	38.0	38.0	38.0	36.0	38.0
78-79	37.035818371752114	38.0	38.0	38.0	36.0	38.0
80-81	37.03425713919379	38.0	38.0	38.0	36.0	38.0
82-83	36.98503228521162	38.0	38.0	38.0	36.0	38.0
84-85	36.96922196340535	38.0	38.0	38.0	36.0	38.0
86-87	36.939725405673485	38.0	38.0	38.0	35.5	38.0
88-89	36.94715312620424	38.0	38.0	38.0	36.0	38.0
90-91	36.936311871354356	38.0	38.0	38.0	35.0	38.0
92-93	36.920001683620114	38.0	38.0	38.0	35.0	38.0
94-95	36.90522120400966	38.0	38.0	38.0	35.0	38.0
96-97	36.8858339137829	38.0	38.0	38.0	35.0	38.0
98-99	36.920882030364425	38.0	38.0	38.0	35.0	38.0
100	35.456102772875056	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	12.0
21	50.0
22	309.0
23	944.0
24	2317.0
25	4922.0
26	9024.0
27	15591.0
28	25638.0
29	38314.0
30	50945.0
31	65444.0
32	83945.0
33	111574.0
34	167289.0
35	303107.0
36	946955.0
37	4627999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17028054110198	19.52378414423437	16.392331165917614	22.913604148746032
2	14.83746875829413	20.870970185988376	41.67345691034539	22.618104145372104
3	18.41688121279335	25.721086471811216	30.669986612073803	25.192045703321636
4	12.854873522262489	17.583989903285786	38.18996008445122	31.371176490000508
5	13.638977786261245	37.14275444411116	35.24395201772935	13.974315751898244
6	30.55831509250147	37.211808284105814	18.38844729411823	13.84142932927449
7	26.965399085080456	32.486228641883955	22.718394977145604	17.82997729588999
8	24.531667158322158	36.57600787309835	20.798173768402993	18.0941512001765
9	25.781035029833987	16.927982592916692	21.333422368424316	35.957560008825006
10-11	24.510170141720728	26.669222035751204	28.41240155528397	20.408206267244093
12-13	25.29354466669476	24.313280709669584	28.73121586816031	21.66195875547535
14-15	22.400449059120124	25.570333280449383	26.69984463273335	25.329373027697144
16-17	21.779607472998197	28.629811923182753	27.413901930842666	22.176678672976383
18-19	21.76550852877672	27.675173959281008	29.39923469071564	21.160082821226627
20-21	22.64382235778045	26.749767867897038	29.416486592889484	21.189923181433027
22-23	22.37787045207514	26.568775028451768	29.282279488154845	21.771075031318247
24-25	22.042357063370257	26.78690011405535	29.38469070514348	21.786052117430913
26-27	21.941230559687444	26.912351506746507	29.351781275916316	21.794636657649736
28-29	21.91041362670337	26.882678155767696	29.405458552733204	21.801449664795726
30-31	22.15345335934887	26.828495279430115	29.291208449915867	21.726842911305148
32-33	21.93203274697041	26.969514008668625	29.23379259656868	21.864660647792284
34-35	22.11806361984936	26.93643418977721	29.21885298780838	21.72664920256505
36-37	22.094081339364358	26.80639934801977	29.199411441364827	21.900107871251045
38-39	21.954756652523137	27.015898339167073	29.261339833604072	21.768005174705717
40-41	22.214308939390435	26.886551162720462	29.09413742885596	21.80500246903315
42-43	22.074639758285773	26.97800195127975	28.903669974769453	22.043688315665023
44-45	22.173830587980518	27.067008940034302	28.74219286500602	22.01696760697916
46-47	22.22879548869175	27.061303677558108	28.50185771977589	22.208043113974256
48-49	22.19773716989044	27.18273180757051	28.43731277347391	22.182218249065137
50-51	22.203074993702547	27.372117802607416	28.2392772723212	22.185529931368837
52-53	22.33931850427989	27.298339483006483	28.11676224039698	22.245579772316642
54-55	22.373745009371387	27.200453654304553	28.14045674962126	22.2853445867028
56-57	22.333790013697136	27.342470651683097	28.097818772771937	22.225920561847825
58-59	22.279387536219257	27.29289931691243	28.198340570664683	22.229372576203634
60-61	22.328783335211924	27.311525288047523	28.204138635159577	22.155552741580983
62-63	22.325021131164476	27.335697491679966	28.223397971208307	22.115883405947244
64-65	22.289856461751764	27.2242948318421	28.22724758131457	22.258601125091566
66-67	22.354397756400466	27.228554639318226	28.12934169762391	22.287705906657393
68-69	22.2675281043316	27.263344879153774	28.22672290294331	22.24240411357131
70-71	22.284796676353047	26.99811645965644	28.260159547450346	22.456927316540167
72-73	22.378926647096417	27.046865354195347	28.214102328668943	22.36010567003929
74-75	22.17540325996805	27.313695784685876	28.133940484316017	22.37696047103006
76-77	22.33402719558184	27.216766402249366	28.03025705306203	22.418949349106768
78-79	22.285747186811143	27.187856709768703	28.02094704848524	22.505449054934918
80-81	22.29331089665721	27.290576248572428	27.96479907887039	22.45131377589998
82-83	22.41691090028553	27.258197929423922	27.98730731345177	22.33758385683878
84-85	22.468923587252007	27.253790069544593	27.89077449081677	22.386511852386633
86-87	22.318693869550064	27.347097152649958	27.94424632830926	22.389962649490723
88-89	22.410580286913774	27.290268214846492	27.904295143371993	22.394856354867738
90-91	22.458994179157585	27.350727330373303	27.85610185663419	22.33417663383492
92-93	22.483320704242896	27.36825100324308	27.816967340692	22.33146095182202
94-95	22.362991183135655	27.375383462393216	27.837968939058598	22.423656415412527
96-97	22.51805999904579	27.50000048290059	27.838822850623973	22.14311666742965
98-99	23.544446131285547	29.407117987491056	28.070900016849876	18.977535864373525
100	22.472500271543776	30.282275539050197	21.94613496816273	25.299089221243293
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1708.0
1	1564.5
2	1688.5
3	2124.0
4	2735.0
5	3873.0
6	5043.5
7	7064.5
8	9185.5
9	9907.5
10	9625.0
11	9330.5
12	10218.0
13	12836.0
14	18219.5
15	24410.0
16	28691.5
17	30465.0
18	29338.0
19	26172.0
20	22446.0
21	19405.5
22	18524.5
23	20363.0
24	24598.0
25	31845.0
26	41857.0
27	53276.0
28	65041.0
29	78174.5
30	92101.0
31	105887.5
32	122748.5
33	140105.0
34	156210.0
35	171916.0
36	188898.5
37	204018.5
38	213856.0
39	222884.5
40	232143.0
41	237587.5
42	239056.5
43	244224.0
44	250550.5
45	254996.5
46	256772.0
47	256611.0
48	258557.5
49	259218.0
50	256444.5
51	250437.0
52	241563.5
53	229167.0
54	214682.5
55	199817.0
56	183370.0
57	165237.5
58	146767.0
59	128151.5
60	108187.0
61	87301.5
62	68103.5
63	52112.0
64	38922.0
65	28693.0
66	21143.5
67	15815.5
68	11936.5
69	8688.5
70	6138.5
71	4265.0
72	2850.0
73	1832.5
74	1097.0
75	614.5
76	357.5
77	220.0
78	146.5
79	91.0
80	55.0
81	36.0
82	27.0
83	16.5
84	9.5
85	6.5
86	4.5
87	4.5
88	5.5
89	6.0
90	5.5
91	3.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004198696575846742
3	9.450940631979751E-4
4	1.0845341708829223E-4
5	0.0
6	0.0
7	7.126938837230632E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.746774068975262E-6
22-23	6.352702672132676E-4
24-25	5.423430039030876E-4
26-27	7.748495610206039E-5
28-29	0.0029993447942922237
30-31	0.0021014851373820707
32-33	0.004541471318706638
34-35	0.009593942226275071
36-37	0.0015348725377956518
38-39	0.0
40-41	1.6473267886870383E-4
42-43	7.875623119301199E-4
44-45	2.3745475101840389E-4
46-47	6.370640520920904E-5
48-49	0.004077856781023177
50-51	0.003334900629224862
52-53	2.9953559838914615E-4
54-55	0.0027420955028738705
56-57	0.04071790510960612
58-59	0.061613914176574816
60-61	0.02864684950476966
62-63	0.010310478661056922
64-65	9.636270991045222E-4
66-67	1.434049860563958E-4
68-69	0.0014358787526988573
70-71	0.010794454749063596
72-73	0.01604097243824592
74-75	0.00970501616402155
76-77	0.011900120945158568
78-79	0.002534379923331034
80-81	0.012987779995558502
82-83	0.04243238383460968
84-85	0.023708941207263634
86-87	0.0039038590149201467
88-89	6.45935758366875E-5
90-91	9.319609773027427E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	46.0
20-21	266.0
22-23	515.0
24-25	500.0
26-27	1131.0
28-29	2489.0
30-31	6827.0
32-33	8798.0
34-35	12019.0
36-37	18863.0
38-39	23060.0
40-41	24308.0
42-43	29636.0
44-45	37253.0
46-47	38570.0
48-49	35115.0
50-51	31203.0
52-53	30563.0
54-55	34052.0
56-57	35305.0
58-59	35752.0
60-61	35729.0
62-63	35914.0
64-65	38815.0
66-67	41896.0
68-69	43799.0
70-71	43737.0
72-73	42643.0
74-75	44040.0
76-77	47175.0
78-79	48943.0
80-81	49262.0
82-83	47539.0
84-85	47458.0
86-87	49657.0
88-89	52714.0
90-91	54377.0
92-93	53752.0
94-95	64281.0
96-97	205982.0
98-99	461537.0
100-101	4538863.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.75313819075612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.15515413392842	77.31087391055681
2	6.9805171175979215	11.972024980566264
3	1.6278548951144882	4.187809973257545
4	0.5835543453761926	2.0016646568344343
5	0.254737979911201	1.092229059687964
6	0.13159325477416173	0.677072073697204
7	0.07652971310009105	0.4593864144219718
8	0.04529056748204051	0.3107046633620148
9	0.03423812654420383	0.26424241172439356
>10	0.10777067605206704	1.4134749064015844
>50	0.001823000297477286	0.10267174831673753
>100	8.507424849407163E-4	0.14900636402358905
>500	5.696489565933238E-5	0.031800819187546014
>1k	2.848244117181168E-5	0.027038017961948088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5493345298327463E-5
2	0.0	3.0986690596654926E-5	0.0	1.5493345298327463E-5	1.5493345298327463E-5
3	0.0	3.0986690596654926E-5	0.0	1.5493345298327463E-5	1.5493345298327463E-5
4	0.0	2.01413488878257E-4	0.0	1.5493345298327463E-5	1.5493345298327463E-5
5	0.0	2.1690683417658447E-4	0.0	1.5493345298327463E-5	1.5493345298327463E-5
6	0.0	2.3240017947491194E-4	0.0	3.0986690596654926E-5	3.0986690596654926E-5
7	0.0	2.3240017947491194E-4	0.0	3.0986690596654926E-5	3.0986690596654926E-5
8	0.0	2.3240017947491194E-4	0.0	3.0986690596654926E-5	3.0986690596654926E-5
9	0.0	3.718402871598591E-4	0.0	3.0986690596654926E-5	3.0986690596654926E-5
10-11	1.5493345298327463E-5	3.718402871598591E-4	0.0	5.422670854414612E-5	3.0986690596654926E-5
12-13	1.5493345298327463E-5	3.7958695980902285E-4	0.0	7.746672649163731E-5	3.0986690596654926E-5
14-15	2.3240017947491195E-5	4.725470315989876E-4	0.0	7.746672649163731E-5	6.197338119330985E-5
16-17	3.0986690596654926E-5	4.957870495464788E-4	0.0	9.296007178996477E-5	7.746672649163731E-5
18-19	3.0986690596654926E-5	5.345204127922974E-4	0.0	1.3944010768494717E-4	7.746672649163731E-5
20-21	3.0986690596654926E-5	5.732537760381161E-4	0.0	1.9366681622909327E-4	7.746672649163731E-5
22-23	3.0986690596654926E-5	6.274804845822623E-4	0.0	2.246535068257482E-4	7.746672649163731E-5
24-25	3.0986690596654926E-5	7.126938837230633E-4	0.0	2.478935247732394E-4	7.746672649163731E-5
26-27	3.0986690596654926E-5	7.591739196180457E-4	0.0	2.943735606682218E-4	7.746672649163731E-5
28-29	3.0986690596654926E-5	7.824139375655368E-4	0.0	4.8029370424815135E-4	7.746672649163731E-5
30-31	3.0986690596654926E-5	9.141073726013203E-4	0.0	9.83827426443794E-4	7.746672649163731E-5
32-33	3.0986690596654926E-5	9.141073726013203E-4	0.0	0.001990894870835079	7.746672649163731E-5
34-35	3.0986690596654926E-5	9.760807537946301E-4	0.0	0.003253602512648767	7.746672649163731E-5
36-37	3.0986690596654926E-5	0.00103805413498794	0.0	0.0053529508005721385	7.746672649163731E-5
38-39	3.0986690596654926E-5	0.0010845341708829222	0.0	0.008436126514939303	7.746672649163731E-5
40-41	3.0986690596654926E-5	0.0011620008973745597	0.0	0.01175170240878138	7.746672649163731E-5
42-43	3.0986690596654926E-5	0.0011929875879712146	0.0	0.015090518320570949	8.521339914080104E-5
44-45	3.0986690596654926E-5	0.0013091876777086707	0.0	0.0187314544656779	9.296007178996478E-5
46-47	3.0986690596654926E-5	0.0013944010768494716	0.0	0.023348471364579487	9.296007178996478E-5
48-49	3.0986690596654926E-5	0.0013944010768494716	0.0	0.027818301483146957	9.296007178996478E-5
50-51	3.0986690596654926E-5	0.0015803212204294013	0.0	0.032497291763241856	9.296007178996478E-5
52-53	3.0986690596654926E-5	0.0016035612383768923	0.0	0.037362202186916674	1.1620008973745597E-4
54-55	3.0986690596654926E-5	0.0016190545836752197	0.0	0.04206443248495906	1.3944010768494717E-4
56-57	3.0986690596654926E-5	0.001642294601622711	0.0	0.046836382836843916	1.3944010768494717E-4
58-59	5.422670854414612E-5	0.0016577879469210385	0.0	0.05138367968190303	1.3944010768494717E-4
60-61	6.197338119330985E-5	0.0016810279648685296	0.0	0.056147883361138726	1.3944010768494717E-4
62-63	6.197338119330985E-5	0.0017507480187110031	0.0	0.06069518020619784	1.5493345298327462E-4
64-65	6.197338119330985E-5	0.0019521615075892603	0.0	0.06472344998376298	1.5493345298327462E-4
66-67	6.197338119330985E-5	0.001967654852887588	0.0	0.06910806670318964	1.5493345298327462E-4
68-69	6.197338119330985E-5	0.0020063882161334063	0.0	0.07377931031063537	1.5493345298327462E-4
70-71	6.197338119330985E-5	0.0020218815614317337	0.0	0.07808646030357042	1.5493345298327462E-4
72-73	6.197338119330985E-5	0.0020296282340808974	0.0	0.08173514312132653	2.01413488878257E-4
74-75	6.197338119330985E-5	0.00207610826997588	0.0	0.08460915867416627	2.01413488878257E-4
76-77	6.197338119330985E-5	0.0022000550323624996	0.0	0.08739796082786522	2.0916016152742073E-4
78-79	6.197338119330985E-5	0.0022620284135558095	0.0	0.0891951888824712	2.1690683417658447E-4
80-81	6.197338119330985E-5	0.0022620284135558095	0.0	0.08984590938500095	2.3240017947491194E-4
82-83	6.197338119330985E-5	0.002269775086204973	0.0	0.09025648303540663	2.478935247732394E-4
84-85	6.197338119330985E-5	0.002277521758854137	0.0	0.09055085659607484	2.478935247732394E-4
86-87	6.197338119330985E-5	0.002308508449450792	0.0	0.09065156334051398	2.478935247732394E-4
88	6.197338119330985E-5	0.0024634419024340665	0.0	0.09065156334051398	2.478935247732394E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9490	0.0	52.01766	1
GTATCAA	17760	0.0	37.990437	1
TCAACGC	22475	0.0	30.38945	4
ATCAACG	22700	0.0	30.030529	3
CAACGCA	22925	0.0	29.773806	5
TATCAAC	23185	0.0	29.611696	2
AACGCAG	23605	0.0	28.897526	6
ACGCAGA	27005	0.0	25.243017	7
GTGGTAT	4955	0.0	25.21621	1
TGGTATC	4660	0.0	25.119095	2
CGCAGAG	27520	0.0	24.802488	8
GCAGAGT	31695	0.0	21.604555	9
GTACATG	21945	0.0	20.616869	1
GAGTACT	18770	0.0	20.611307	12-13
TACATGG	22430	0.0	20.581532	2
ACATGGG	22530	0.0	20.158443	3
AGAGTAC	28995	0.0	19.345156	10-11
CAGAGTA	30865	0.0	19.181536	10-11
GTACTTT	20245	0.0	18.990889	14-15
CATGGGG	15195	0.0	18.175858	4
>>END_MODULE