FastQCFastQC Report
Fri 27 May 2016
SRR2139590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7252818
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101900.051.7449231
GTATCAA186600.036.5695881
TCAACGC233800.029.3310364
ATCAACG236500.029.0144443
CAACGCA239400.028.6627755
TATCAAC241750.028.4921592
AACGCAG246150.027.8941356
GTGGTAT46200.025.341681
TGGTATC45000.025.1628672
ACGCAGA282700.024.3028437
CGCAGAG292400.023.525858
GCAGAGT332300.020.6753379
GAGTACT194600.020.3422712-13
TACATGG235500.019.4867842
GTACATG233900.019.455671
CAGAGTA325000.019.0165610-11
ACATGGG236450.018.8471153
AGAGTAC303250.018.4361310-11
GTACTTT216800.018.20025414-15
CATGGGG166750.017.2186974
Produced by FastQC (version 0.11.5)