FastQCFastQC Report
Fri 27 May 2016
SRR2139589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7177798
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111000.047.6681821
GTATCAA192850.036.817151
TCAACGC229800.030.1553464
ATCAACG231250.030.0036143
CAACGCA235350.029.4442235
TATCAAC241350.029.084332
AACGCAG242650.028.4709176
ACGCAGA276250.024.9004347
CGCAGAG284250.024.214238
GTGGTAT54600.023.099221
TGGTATC54700.022.203032
GCAGAGT325400.021.2564959
GAGTACT191550.020.62634812-13
GTACATG220350.019.6764261
CTTATAC80450.019.160821
CAGAGTA315500.019.09726310-11
TACATGG223400.018.7805062
GTACTTT211950.018.49102214-15
AGAGTAC292950.018.11051810-11
ACATGGG223900.017.902813
Produced by FastQC (version 0.11.5)