##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7177798
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.77977284955637	33.0	31.0	33.0	25.0	34.0
2	32.09434383636876	33.0	31.0	33.0	29.0	34.0
3	32.113871412931935	33.0	31.0	33.0	29.0	34.0
4	32.826031465360266	33.0	33.0	34.0	31.0	34.0
5	32.92741548313285	33.0	33.0	34.0	31.0	34.0
6	36.56254021080002	38.0	37.0	38.0	34.0	38.0
7	37.06350749909652	38.0	38.0	38.0	36.0	38.0
8	36.9516215140075	38.0	38.0	38.0	35.0	38.0
9	37.1461223901815	38.0	38.0	38.0	36.0	38.0
10-11	37.28229660405601	38.0	38.0	38.0	36.0	38.0
12-13	37.295374430988446	38.0	38.0	38.0	37.0	38.0
14-15	37.37810091618627	38.0	38.0	38.0	37.0	38.0
16-17	37.39223554354692	38.0	38.0	38.0	37.0	38.0
18-19	37.40898977653035	38.0	38.0	38.0	37.0	38.0
20-21	37.424621644287264	38.0	38.0	38.0	37.0	38.0
22-23	37.43871529332151	38.0	38.0	38.0	37.0	38.0
24-25	37.452174707986856	38.0	38.0	38.0	37.0	38.0
26-27	37.429119654431446	38.0	38.0	38.0	37.0	38.0
28-29	37.40287686918826	38.0	38.0	38.0	37.0	38.0
30-31	37.407255714170454	38.0	38.0	38.0	37.0	38.0
32-33	37.39292930652688	38.0	38.0	38.0	37.0	38.0
34-35	37.36508917464051	38.0	38.0	38.0	37.0	38.0
36-37	37.3484060841133	38.0	38.0	38.0	37.0	38.0
38-39	37.320097124201865	38.0	38.0	38.0	37.0	38.0
40-41	37.32220667343277	38.0	38.0	38.0	37.0	38.0
42-43	37.29395191143665	38.0	38.0	38.0	37.0	38.0
44-45	37.25433291895803	38.0	38.0	38.0	37.0	38.0
46-47	37.20958972831218	38.0	38.0	38.0	37.0	38.0
48-49	37.152652006868934	38.0	38.0	38.0	36.5	38.0
50-51	37.07320340827671	38.0	38.0	38.0	36.0	38.0
52-53	37.09264538596297	38.0	38.0	38.0	36.0	38.0
54-55	37.06852386485055	38.0	38.0	38.0	36.0	38.0
56-57	37.01172110738176	38.0	38.0	38.0	36.0	38.0
58-59	36.99316154080769	38.0	38.0	38.0	36.0	38.0
60-61	37.00956984588242	38.0	38.0	38.0	36.0	38.0
62-63	37.00662214826403	38.0	38.0	38.0	36.0	38.0
64-65	36.94510570475335	38.0	38.0	38.0	36.0	38.0
66-67	36.98527158375394	38.0	38.0	38.0	36.0	38.0
68-69	36.993937035151326	38.0	38.0	38.0	36.0	38.0
70-71	36.97117440564626	38.0	38.0	38.0	36.0	38.0
72-73	36.9609171921325	38.0	38.0	38.0	36.0	38.0
74-75	36.913656636880866	38.0	38.0	38.0	35.5	38.0
76-77	36.91440460301339	38.0	38.0	38.0	35.0	38.0
78-79	36.91053676780956	38.0	38.0	38.0	35.0	38.0
80-81	36.905202381653694	38.0	38.0	38.0	35.0	38.0
82-83	36.84160581839306	38.0	38.0	38.0	35.0	38.0
84-85	36.825043783304224	38.0	38.0	38.0	35.0	38.0
86-87	36.79134937468051	38.0	38.0	38.0	35.0	38.0
88-89	36.797452877871166	38.0	38.0	38.0	35.0	38.0
90-91	36.782194241493144	38.0	38.0	38.0	35.0	38.0
92-93	36.75638991086416	38.0	38.0	38.0	35.0	38.0
94-95	36.73528507919178	38.0	38.0	38.0	34.5	38.0
96-97	36.713205901907244	38.0	38.0	38.0	34.0	38.0
98-99	36.75105326016288	38.0	38.0	38.0	34.0	38.0
100	35.17445122911962	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	5.0
20	11.0
21	57.0
22	305.0
23	1037.0
24	2670.0
25	5725.0
26	10565.0
27	18074.0
28	29045.0
29	42618.0
30	58183.0
31	76086.0
32	98548.0
33	132185.0
34	196630.0
35	351071.0
36	974217.0
37	5180763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.33627053868052	19.56540153400806	14.192764967751948	24.905562959559465
2	14.54116179714416	19.89406517539978	42.72728741979026	22.837485607665805
3	18.403097923989684	25.047529086574965	29.936943677022338	26.61242931241301
4	12.70305002042412	17.474728983358105	38.35132773495121	31.470893261266564
5	13.85749501448773	36.49388015656055	35.28640677823478	14.362218050716946
6	30.72759918849764	36.92452755009266	18.347771837546837	14.000101423862862
7	27.185079972999837	32.24767980428366	22.281374277540202	18.2858659451763
8	24.507947980703833	36.41627975599202	20.764947690085457	18.310824573218696
9	25.489237785738744	17.073578832951274	21.125016335093296	36.31216704621668
10-11	24.37078892440272	26.498572403402825	28.410384354644698	20.720254317549756
12-13	25.214780354643583	24.02620552988535	28.646570995728776	22.112443119742295
14-15	22.366546119018675	25.370719543793236	26.435704933462883	25.82702940372521
16-17	21.916359306851486	28.63676297382568	27.12648781701575	22.320389902307085
18-19	21.91236086610406	27.514531615406284	29.054704520801504	21.51840299768815
20-21	22.633442993134388	26.62064465919431	29.05886506355211	21.687047284119195
22-23	22.393183138028164	26.616597075382632	28.94258496137182	22.047634825217386
24-25	22.202063580680562	26.746167425367794	29.046224299212582	22.00554469473906
26-27	22.094625791645655	26.926341816993922	29.000811243859403	21.978221147501024
28-29	21.966767036704198	26.802585684615917	29.101208274915834	22.129439003764052
30-31	22.343130157042793	26.761749727695367	28.99404388804456	21.901076227217285
32-33	22.11711727475253	26.939086894839136	28.961283562889278	21.982512267519052
34-35	22.231695119770745	26.905722649197084	29.022987819617242	21.839594411414932
36-37	22.20695097435524	26.764081375877108	28.916805190973033	22.11216245879462
38-39	21.962416044265254	26.822982240667812	29.120515888132463	22.094085826934464
40-41	22.192698354854564	26.701830615699617	29.02263408267584	22.082836946769977
42-43	22.1680334158835	26.752406432139658	28.88967267911428	22.189887472862566
44-45	22.132146531530847	26.948189369865528	28.74272838902177	22.176935709581855
46-47	22.331657997980734	26.874337522539914	28.35460390240173	22.439400577077617
48-49	22.25725909965907	26.914511385174645	28.3402598114408	22.487969703725486
50-51	22.151279578536602	27.15704271895619	28.29797195279133	22.393705749715878
52-53	22.287486121449703	27.09744001000633	28.2928636198992	22.32221024864477
54-55	22.277860194419713	27.05338991171783	28.164232983423137	22.504516910439325
56-57	22.3407369932202	27.175407015332976	28.051068402113994	22.432787589332833
58-59	22.157237207948896	27.244727698448024	28.297816298020667	22.300218795582413
60-61	22.267539784691596	27.403207327304425	28.081611082078656	22.247641805925326
62-63	22.15634327028055	27.521237076086024	28.07110351314221	22.251316140491216
64-65	22.125470105378984	27.443171146763586	28.084854075229025	22.346504672628402
66-67	22.152635445317312	27.465970938909468	27.92887670821217	22.452516907561044
68-69	22.121094715950417	27.380738160808253	28.100920586617224	22.397246536624106
70-71	22.155956215813447	27.12227901519605	28.14500023849902	22.576764530491488
72-73	22.310652155170427	27.016492600229427	28.09952390323007	22.57333134137007
74-75	22.18756100735342	27.199294339978458	28.004267692630513	22.60887696003761
76-77	22.415544420444146	27.10417501897751	27.888221411390727	22.592059149187616
78-79	22.36665883627531	27.136305804011222	27.789991271565924	22.707044088147544
80-81	22.346343142286905	27.21226675730633	27.79134146414367	22.650048636263094
82-83	22.479029359414337	27.186387446838093	27.811909923107365	22.522673270640205
84-85	22.48290761652901	27.21192222904595	27.76469607273811	22.540474081686938
86-87	22.39553834067199	27.26044897678099	27.83380051972583	22.51021216282119
88-89	22.48765001166474	27.276970609289748	27.743196529141006	22.49218284990451
90-91	22.498639405540327	27.300820802359944	27.7092590693749	22.49128072272483
92-93	22.602816215615505	27.271339056915835	27.735219686428398	22.390625041040263
94-95	22.454126357405002	27.357894975834583	27.72229875763615	22.465679909124265
96-97	22.61295132905984	27.396601690944973	27.792733587144603	22.197713392850584
98-99	23.641787204618367	29.316129706608347	28.01513391170109	19.026949177072193
100	22.570407867964555	30.177874634881018	21.926531160753566	25.32518633640086
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1855.0
1	1649.0
2	1729.0
3	2183.5
4	2736.0
5	3835.0
6	5025.5
7	6677.0
8	8846.0
9	10021.0
10	9730.0
11	9355.0
12	10215.5
13	12829.5
14	18271.0
15	24609.5
16	29192.5
17	31681.5
18	31373.0
19	28685.5
20	25290.5
21	22601.5
22	22182.5
23	24610.5
24	29748.0
25	37888.5
26	48778.5
27	61018.0
28	74031.5
29	89207.0
30	104015.5
31	118654.5
32	136809.5
33	155395.5
34	172960.5
35	190080.0
36	209518.5
37	227433.5
38	240590.5
39	252669.0
40	264194.0
41	274133.0
42	279854.0
43	287390.0
44	298784.5
45	311220.0
46	314196.5
47	311851.5
48	312159.5
49	308470.0
50	303852.5
51	296238.5
52	285130.0
53	270908.5
54	253525.0
55	236457.0
56	217814.0
57	198076.0
58	178214.0
59	155117.5
60	130522.0
61	106851.0
62	85116.5
63	66375.5
64	50041.0
65	37039.5
66	27714.5
67	21318.0
68	16456.0
69	12222.5
70	8997.0
71	6517.5
72	4591.5
73	3059.5
74	1947.0
75	1244.0
76	792.5
77	489.0
78	323.5
79	232.0
80	164.0
81	110.5
82	75.0
83	54.5
84	38.5
85	24.0
86	15.0
87	12.5
88	11.5
89	7.5
90	3.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005099056841666483
3	0.0012260027378870233
4	1.3931849294170718E-4
5	0.0
6	0.0
7	8.777065055327554E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.409193570185547E-4
24-25	5.99181838101764E-4
26-27	1.254283727341016E-4
28-29	0.002614339898347493
30-31	0.001759137620477367
32-33	0.004220226390300394
34-35	0.009170858858656419
36-37	0.0013405967334737762
38-39	0.0
40-41	1.1446119146803418E-4
42-43	5.90841438909301E-4
44-45	2.470519616398059E-4
46-47	4.402146339817083E-5
48-49	0.004243846219307012
50-51	0.0030702935872996525
52-53	5.268045294051376E-4
54-55	0.0027140148543036468
56-57	0.04009795236891888
58-59	0.06203526021038386
60-61	0.02879366206990778
62-63	0.010450467642759146
64-65	7.664327124227524E-4
66-67	1.993835698049056E-4
68-69	0.001345138656812197
70-71	0.010727234903003985
72-73	0.01632737396521669
74-75	0.010216650251693944
76-77	0.01245466547832239
78-79	0.0027312606137590762
80-81	0.01330381332717612
82-83	0.04459866845429188
84-85	0.024755979569747167
86-87	0.0038208507602922083
88-89	7.096414908929047E-5
90-91	8.963072053508824E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	79.0
20-21	380.0
22-23	766.0
24-25	781.0
26-27	2546.0
28-29	6486.0
30-31	17549.0
32-33	22142.0
34-35	30143.0
36-37	45537.0
38-39	49860.0
40-41	49297.0
42-43	55101.0
44-45	65563.0
46-47	65581.0
48-49	59290.0
50-51	51149.0
52-53	47647.0
54-55	50927.0
56-57	51114.0
58-59	54495.0
60-61	55491.0
62-63	54051.0
64-65	57516.0
66-67	61075.0
68-69	62319.0
70-71	68300.0
72-73	54788.0
74-75	54307.0
76-77	55943.0
78-79	57771.0
80-81	56731.0
82-83	53648.0
84-85	53259.0
86-87	55170.0
88-89	57928.0
90-91	58362.0
92-93	57591.0
94-95	68212.0
96-97	219422.0
98-99	486562.0
100-101	4702919.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.28542434841647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.03559885960576	79.46080877272907
2	6.483898875036694	11.31899729479596
3	1.4333758948066373	3.753384696869655
4	0.517787069749597	1.8078105642086677
5	0.2149833685370325	0.9382457275303444
6	0.10729697564742155	0.561927723041214
7	0.059355644021831314	0.3626617803143367
8	0.037941356751454276	0.26493819395242735
9	0.026655039837472704	0.2093936816913976
>10	0.08097112379877068	1.1170804746746161
>50	0.0016866559330004243	0.09876627903619073
>100	3.774830046373919E-4	0.05238558601383955
>500	4.776885770700358E-5	0.029520235212169926
>1k	2.3884411956176407E-5	0.024078989930078728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3931849294170719E-5	0.0
2	0.0	1.3931849294170719E-5	0.0	1.3931849294170719E-5	0.0
3	0.0	1.3931849294170719E-5	0.0	1.3931849294170719E-5	0.0
4	0.0	5.5727397176682876E-5	0.0	4.179554788251216E-5	0.0
5	0.0	6.965924647085359E-5	0.0	4.179554788251216E-5	0.0
6	0.0	6.965924647085359E-5	0.0	6.965924647085359E-5	0.0
7	0.0	6.965924647085359E-5	0.0	6.965924647085359E-5	0.0
8	0.0	8.359109576502432E-5	0.0	8.359109576502432E-5	0.0
9	1.3931849294170719E-5	1.8111404082421935E-4	0.0	9.752294505919504E-5	0.0
10-11	2.7863698588341438E-5	1.8111404082421935E-4	0.0	1.2538664364753648E-4	0.0
12-13	2.7863698588341438E-5	2.0897773941256078E-4	0.0	1.3235256829462183E-4	0.0
14-15	2.7863698588341438E-5	3.204325337659266E-4	0.0	1.5325034223587791E-4	3.4829623235426796E-5
16-17	2.7863698588341438E-5	3.343643830600973E-4	0.0	1.74148116177134E-4	4.179554788251216E-5
18-19	3.4829623235426796E-5	3.343643830600973E-4	0.0	1.9504589011839005E-4	4.876147252959752E-5
20-21	5.5727397176682876E-5	3.343643830600973E-4	0.0	2.577392119421583E-4	5.5727397176682876E-5
22-23	6.965924647085359E-5	3.622280816484387E-4	1.3931849294170719E-5	3.343643830600973E-4	5.5727397176682876E-5
24-25	8.359109576502432E-5	3.622280816484387E-4	1.3931849294170719E-5	4.597510267076337E-4	5.5727397176682876E-5
26-27	8.359109576502432E-5	3.622280816484387E-4	1.3931849294170719E-5	5.43342122472658E-4	5.5727397176682876E-5
28-29	8.359109576502432E-5	3.622280816484387E-4	1.3931849294170719E-5	0.0011006160942394869	5.5727397176682876E-5
30-31	8.359109576502432E-5	4.1795547882512155E-4	1.3931849294170719E-5	0.003016245372187961	6.269332182376824E-5
32-33	8.359109576502432E-5	4.1795547882512155E-4	1.3931849294170719E-5	0.006624594339378177	7.662517111793896E-5
34-35	8.359109576502432E-5	5.363761978255726E-4	1.3931849294170719E-5	0.011521639366279184	1.1145479435336575E-4
36-37	9.752294505919504E-5	6.547969168260238E-4	1.3931849294170719E-5	0.019295611272426447	1.1145479435336575E-4
38-39	1.044888697062804E-4	7.174902386497921E-4	1.3931849294170719E-5	0.03985902083062243	1.1145479435336575E-4
40-41	1.1145479435336575E-4	8.637746562385846E-4	1.3931849294170719E-5	0.0622823322695902	1.1145479435336575E-4
42-43	1.1145479435336575E-4	8.846724301798407E-4	1.3931849294170719E-5	0.07482099663434386	1.1145479435336575E-4
44-45	1.1145479435336575E-4	9.89161299886121E-4	1.3931849294170719E-5	0.08931708582492848	1.1145479435336575E-4
46-47	1.1145479435336575E-4	0.0010797183202982308	1.3931849294170719E-5	0.10385497056339563	1.1145479435336575E-4
48-49	1.2538664364753648E-4	0.001086684244945316	1.3931849294170719E-5	0.11831623013074483	1.3931849294170718E-4
50-51	1.3931849294170718E-4	0.0012399345871811941	1.3931849294170719E-5	0.13149575956303033	1.3931849294170718E-4
52-53	1.3931849294170718E-4	0.0012538664364753647	1.3931849294170719E-5	0.17596622251002328	1.4628441758879254E-4
54-55	1.3931849294170718E-4	0.0012677982857695353	1.3931849294170719E-5	0.22960384229258055	1.602162668829633E-4
56-57	1.3931849294170718E-4	0.0012886960597107916	2.089777394125608E-5	0.3070788562174639	1.880799654713047E-4
58-59	1.3931849294170718E-4	0.001316559758299133	2.7863698588341438E-5	0.3554363051175305	2.0897773941256078E-4
60-61	1.3931849294170718E-4	0.0013722871554758158	2.7863698588341438E-5	0.37276752563947885	2.0897773941256078E-4
62-63	1.3931849294170718E-4	0.001393184929417072	2.7863698588341438E-5	0.38662274976253164	2.3684143800090224E-4
64-65	1.3931849294170718E-4	0.0015603671209471206	2.7863698588341438E-5	0.39400662988844215	2.3684143800090224E-4
66-67	1.5325034223587791E-4	0.0015742989702412912	2.7863698588341438E-5	0.400359553166584	2.3684143800090224E-4
68-69	1.5325034223587791E-4	0.001623060442770889	2.7863698588341438E-5	0.4069075223348442	2.3684143800090224E-4
70-71	1.5325034223587791E-4	0.00165092414135923	2.7863698588341438E-5	0.4120762384229815	2.3684143800090224E-4
72-73	1.5325034223587791E-4	0.0016578900660063156	2.7863698588341438E-5	0.4157542466366426	2.5077328729507296E-4
74-75	1.5325034223587791E-4	0.0016996856138888276	2.7863698588341438E-5	0.4190839586179494	2.5077328729507296E-4
76-77	1.5325034223587791E-4	0.0017484470864184251	2.7863698588341438E-5	0.4224902957703741	2.5077328729507296E-4
78-79	1.5325034223587791E-4	0.0017693448603596812	2.7863698588341438E-5	0.4246218687123823	2.5077328729507296E-4
80-81	1.5325034223587791E-4	0.0017763107850067668	2.7863698588341438E-5	0.4253323930263849	2.5077328729507296E-4
82-83	1.5325034223587791E-4	0.001783276709653852	2.7863698588341438E-5	0.4257921440530926	2.6470513658924367E-4
84-85	1.5325034223587791E-4	0.0017972085589480229	2.7863698588341438E-5	0.4260289854910935	2.6470513658924367E-4
86-87	1.5325034223587791E-4	0.0018111404082421935	2.7863698588341438E-5	0.42613347436079974	2.6470513658924367E-4
88	1.5325034223587791E-4	0.0018668678054188765	2.7863698588341438E-5	0.4261613380593881	2.6470513658924367E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11100	0.0	47.668182	1
GTATCAA	19285	0.0	36.81715	1
TCAACGC	22980	0.0	30.155346	4
ATCAACG	23125	0.0	30.003614	3
CAACGCA	23535	0.0	29.444223	5
TATCAAC	24135	0.0	29.08433	2
AACGCAG	24265	0.0	28.470917	6
ACGCAGA	27625	0.0	24.900434	7
CGCAGAG	28425	0.0	24.21423	8
GTGGTAT	5460	0.0	23.09922	1
TGGTATC	5470	0.0	22.20303	2
GCAGAGT	32540	0.0	21.256495	9
GAGTACT	19155	0.0	20.626348	12-13
GTACATG	22035	0.0	19.676426	1
CTTATAC	8045	0.0	19.16082	1
CAGAGTA	31550	0.0	19.097263	10-11
TACATGG	22340	0.0	18.780506	2
GTACTTT	21195	0.0	18.491022	14-15
AGAGTAC	29295	0.0	18.110518	10-11
ACATGGG	22390	0.0	17.90281	3
>>END_MODULE