FastQCFastQC Report
Fri 27 May 2016
SRR2139588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5793331
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80300.049.6552541
GTATCAA149900.037.942641
TCAACGC177550.031.4277674
ATCAACG179300.031.0735283
CAACGCA182950.030.4530625
TATCAAC183650.030.4334132
AACGCAG189250.029.43936
TGGTATC38100.027.31572
GTGGTAT41600.026.7677021
ACGCAGA218150.025.5785127
CGCAGAG224550.024.8492728
GCAGAGT255700.021.8555689
GTACATG190350.020.4072951
GAGTACT149200.020.00535812-13
TACATGG190550.019.8239732
ACATGGG191300.019.207283
CAGAGTA252600.018.93660510-11
GTACTTT160650.018.57959414-15
AGAGTAC233100.018.31619310-11
AGTACTT161150.016.68828412-13
Produced by FastQC (version 0.11.5)