##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5793331
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.64674778637713	33.0	31.0	33.0	18.0	34.0
2	32.037159623712164	33.0	31.0	33.0	29.0	34.0
3	32.087562405807645	33.0	31.0	33.0	29.0	34.0
4	32.80306114047342	33.0	33.0	34.0	31.0	34.0
5	32.90650784496864	33.0	33.0	34.0	31.0	34.0
6	36.47215738234187	38.0	37.0	38.0	34.0	38.0
7	37.01536784278336	38.0	38.0	38.0	35.0	38.0
8	36.88605777919473	38.0	38.0	38.0	35.0	38.0
9	37.093748139023994	38.0	38.0	38.0	36.0	38.0
10-11	37.23962889398172	38.0	38.0	38.0	36.0	38.0
12-13	37.25531227198998	38.0	38.0	38.0	36.0	38.0
14-15	37.345018090628685	38.0	38.0	38.0	37.0	38.0
16-17	37.36355181500936	38.0	38.0	38.0	37.0	38.0
18-19	37.38158884759045	38.0	38.0	38.0	37.0	38.0
20-21	37.399223859691176	38.0	38.0	38.0	37.0	38.0
22-23	37.41283640064742	38.0	38.0	38.0	37.0	38.0
24-25	37.42901462077015	38.0	38.0	38.0	37.0	38.0
26-27	37.40793151410912	38.0	38.0	38.0	37.0	38.0
28-29	37.383604148718035	38.0	38.0	38.0	37.0	38.0
30-31	37.38831935373345	38.0	38.0	38.0	37.0	38.0
32-33	37.37701468717898	38.0	38.0	38.0	37.0	38.0
34-35	37.35038933841969	38.0	38.0	38.0	37.0	38.0
36-37	37.33345687047275	38.0	38.0	38.0	37.0	38.0
38-39	37.31941620770249	38.0	38.0	38.0	37.0	38.0
40-41	37.30236111211643	38.0	38.0	38.0	37.0	38.0
42-43	37.27318336907636	38.0	38.0	38.0	37.0	38.0
44-45	37.23640138002967	38.0	38.0	38.0	37.0	38.0
46-47	37.193093562053534	38.0	38.0	38.0	37.0	38.0
48-49	37.13474929668862	38.0	38.0	38.0	36.0	38.0
50-51	37.056801508956426	38.0	38.0	38.0	36.0	38.0
52-53	37.07636607553359	38.0	38.0	38.0	36.0	38.0
54-55	37.049778166904005	38.0	38.0	38.0	36.0	38.0
56-57	36.9906848141143	38.0	38.0	38.0	36.0	38.0
58-59	36.97257411230029	38.0	38.0	38.0	36.0	38.0
60-61	36.99138469420934	38.0	38.0	38.0	36.0	38.0
62-63	36.98606871487528	38.0	38.0	38.0	36.0	38.0
64-65	36.92536315650644	38.0	38.0	38.0	36.0	38.0
66-67	36.962836113650326	38.0	38.0	38.0	36.0	38.0
68-69	36.969239849546135	38.0	38.0	38.0	36.0	38.0
70-71	36.94521836804024	38.0	38.0	38.0	36.0	38.0
72-73	36.93204725478371	38.0	38.0	38.0	36.0	38.0
74-75	36.88282150065099	38.0	38.0	38.0	35.0	38.0
76-77	36.884210437390045	38.0	38.0	38.0	35.0	38.0
78-79	36.87849515438049	38.0	38.0	38.0	35.0	38.0
80-81	36.87262182835707	38.0	38.0	38.0	35.0	38.0
82-83	36.81136298788823	38.0	38.0	38.0	35.0	38.0
84-85	36.78818119831993	38.0	38.0	38.0	35.0	38.0
86-87	36.75121383973356	38.0	38.0	38.0	35.0	38.0
88-89	36.75582867845819	38.0	38.0	38.0	35.0	38.0
90-91	36.73711354449506	38.0	38.0	38.0	34.5	38.0
92-93	36.711323274878275	38.0	38.0	38.0	34.0	38.0
94-95	36.691442821008124	38.0	38.0	38.0	34.0	38.0
96-97	36.66953744634361	38.0	38.0	38.0	34.0	38.0
98-99	36.70934988829937	38.0	38.0	38.0	34.0	38.0
100	35.13816891228701	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	13.0
21	58.0
22	299.0
23	915.0
24	2424.0
25	4890.0
26	8977.0
27	15110.0
28	23945.0
29	35487.0
30	47833.0
31	63289.0
32	82408.0
33	110831.0
34	165684.0
35	294423.0
36	802108.0
37	4134633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73272681985545	19.44963959421618	14.201277296256679	24.616356289671693
2	14.76770808033363	19.90142915809812	42.4306545616936	22.90020819987464
3	18.656212229854454	24.8906747938037	29.688141369652282	26.764971606689556
4	12.93589248987842	17.206452609602618	38.26881500488062	31.588839895638337
5	14.028992992114553	36.604278264093665	35.02047440410362	14.34625433968817
6	31.19901141502186	36.66893881948054	18.12430534350618	14.007744421991426
7	27.616624448174825	32.29209730247572	22.099710440122383	17.991567809227078
8	24.82005602648977	36.35702845219788	20.458903521997968	18.364011999314382
9	25.778606470094665	17.143591484760666	20.96260337964463	36.11519866550004
10-11	24.708513979263397	26.466992823299755	28.114663222246406	20.709829975190438
12-13	25.491224306016697	23.996807708725775	28.405022947937898	22.106945037319637
14-15	22.612422801321035	25.329279476694843	26.279225198767342	25.779072523216783
16-17	22.066234434041487	28.543552923180123	27.016184644032943	22.374027998745454
18-19	22.086844338775048	27.557004769794784	28.91605019633782	21.44010069509234
20-21	22.932961682193223	26.6073263065882	28.883511171154204	21.576200840064374
22-23	22.70885275751773	26.45028641228627	28.776092622779746	22.06476820741625
24-25	22.427239954391524	26.561394958466302	28.866549632881817	22.14481545426036
26-27	22.24725724357825	26.778027283866322	28.840327335949794	22.134388136605633
28-29	22.213712200865984	26.75650036369114	28.92734174858138	22.1024456868615
30-31	22.44949037789689	26.625958799689386	28.816926643362052	22.107624179051676
32-33	22.228760135317476	26.79826758009167	28.755578415149223	22.21739386944163
34-35	22.434037708921238	26.76438426782645	28.740275376542336	22.061302646709972
36-37	22.397447946865718	26.674619156167427	28.65085378932026	22.2770791076466
38-39	22.22509558563074	26.856338476907254	28.765033475365986	22.153532462096017
40-41	22.5103241323007	26.7350676286931	28.617497407382892	22.13711083162331
42-43	22.379542837034432	26.762203013226532	28.46702284518083	22.39123130455821
44-45	22.419144242317294	26.88293706172305	28.360654608121134	22.337264087838523
46-47	22.5277817924913	26.86962149381226	28.109814396258155	22.492782317438287
48-49	22.422031766008104	26.98489741818657	28.064411698684168	22.528659117121162
50-51	22.42630219391171	27.206298782991883	27.922555230557432	22.444843792538972
52-53	22.629126867643144	27.09685631236069	27.780511824670644	22.49350499532552
54-55	22.612058604019772	27.011726184774197	27.816354285855788	22.559860925350243
56-57	22.52599590806845	27.12905762176443	27.834255658545644	22.510690811621476
58-59	22.512073866995888	27.07298244618201	27.870770598892186	22.544173087929916
60-61	22.563576027228386	27.073005718853455	27.920698794275538	22.44271945964262
62-63	22.523632762360364	27.132079068201627	27.897352838544748	22.44693533089326
64-65	22.52088450137445	27.0474132191798	27.877864292647093	22.55383798679866
66-67	22.574397250019146	27.12621061827684	27.692057937764414	22.6073341939396
68-69	22.536592683592254	27.08673705940146	27.8403831202522	22.53628713675409
70-71	22.50429652231386	26.875537935735405	27.913159514682985	22.707006027267745
72-73	22.590501642536946	26.901991732257898	27.826945756581317	22.680560868623843
74-75	22.41885184932832	27.124150218929138	27.73001834390174	22.726979587840805
76-77	22.622559303020378	26.984137199934903	27.69144909294352	22.701854404101198
78-79	22.555232553043187	27.018758540777572	27.59146952988817	22.834539376291076
80-81	22.559199323492845	27.08980374189115	27.564927324329418	22.786069610286592
82-83	22.652721036768174	27.06576664637105	27.606405785892846	22.675106530967934
84-85	22.679628688786842	27.060982960303125	27.53856112764879	22.72082722326124
86-87	22.54849902812304	27.102285255476893	27.634562263231548	22.714653453168516
88-89	22.629260094647073	27.097833530086458	27.57065774185406	22.702248633412413
90-91	22.65340086862325	27.082933359339844	27.578296139214125	22.68536963282278
92-93	22.688764001861262	27.077943008332028	27.587347713131997	22.645945276674713
94-95	22.518786547086105	27.161018497136563	27.585207966123832	22.734986989653507
96-97	22.681407550199783	27.296533095612773	27.574726062309956	22.447333291877484
98-99	23.76163016489756	29.20049499331563	27.794120083120593	19.243754758666217
100	22.708945052923756	30.059192719794282	21.670448481497857	25.561413745784105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1564.0
1	1391.5
2	1327.0
3	1549.5
4	1982.5
5	2832.0
6	3779.0
7	5194.0
8	6915.5
9	7776.0
10	7572.0
11	7363.0
12	8120.0
13	10117.0
14	14074.5
15	18845.0
16	22516.0
17	24332.0
18	23985.0
19	22094.5
20	19726.5
21	17729.0
22	17179.5
23	18793.0
24	22461.5
25	28855.5
26	37603.5
27	47363.0
28	57018.0
29	68235.0
30	80054.5
31	91602.0
32	105437.5
33	119933.5
34	134278.0
35	148562.0
36	164446.0
37	177760.0
38	186853.5
39	196235.0
40	206438.5
41	215703.5
42	220989.0
43	226561.0
44	233293.0
45	238656.0
46	241604.5
47	241997.5
48	242947.5
49	244064.5
50	243291.5
51	238766.5
52	232137.5
53	222554.5
54	209656.5
55	197067.0
56	182630.5
57	166479.5
58	150211.0
59	131923.0
60	112266.0
61	92506.5
62	73596.5
63	57206.0
64	43468.5
65	32308.5
66	24012.5
67	18333.5
68	14068.5
69	10529.5
70	7748.0
71	5668.0
72	4028.5
73	2660.5
74	1717.5
75	1111.5
76	695.5
77	426.0
78	276.5
79	178.0
80	125.0
81	82.0
82	52.0
83	33.0
84	20.0
85	15.5
86	13.0
87	9.5
88	8.0
89	6.0
90	4.5
91	3.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005868817093309531
3	0.0011219797384268222
4	2.7617962792044853E-4
5	0.0
6	0.0
7	9.666286977215698E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.63073851330696E-6
22-23	6.646053543196716E-4
24-25	8.632008748713507E-4
26-27	1.7266163697562769E-4
28-29	0.003160929665687642
30-31	0.002195890744925677
32-33	0.004677969854642482
34-35	0.010189341063218216
36-37	0.0016222187452085675
38-39	0.0
40-41	1.3238878349270737E-4
42-43	7.727118669358192E-4
44-45	2.4161298682332254E-4
46-47	7.221965391078002E-5
48-49	0.004152365969867482
50-51	0.0031850147534105587
52-53	2.773067670985504E-4
54-55	0.002653102146666838
56-57	0.041843939832522936
58-59	0.0645257358415645
60-61	0.029995387744477423
62-63	0.01060566004094707
64-65	8.134205875395005E-4
66-67	1.2698093928191498E-4
68-69	0.0014192942007638957
70-71	0.01103106652707908
72-73	0.016730228823765556
74-75	0.010527981299065833
76-77	0.012574078277161286
78-79	0.002547150645914092
80-81	0.013434182036915675
82-83	0.04526594452151163
84-85	0.02447322082019463
86-87	0.003921210664150235
88-89	6.491712841679134E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	44.0
20-21	253.0
22-23	546.0
24-25	573.0
26-27	1637.0
28-29	4139.0
30-31	11186.0
32-33	14449.0
34-35	20251.0
36-37	31735.0
38-39	34767.0
40-41	34843.0
42-43	39923.0
44-45	47878.0
46-47	48786.0
48-49	44713.0
50-51	39320.0
52-53	37279.0
54-55	40317.0
56-57	41628.0
58-59	42524.0
60-61	41638.0
62-63	40791.0
64-65	43806.0
66-67	45793.0
68-69	46339.0
70-71	45429.0
72-73	42883.0
74-75	43592.0
76-77	45833.0
78-79	47298.0
80-81	46871.0
82-83	44057.0
84-85	44073.0
86-87	45252.0
88-89	47433.0
90-91	48627.0
92-93	48302.0
94-95	56687.0
96-97	180440.0
98-99	402274.0
100-101	3849122.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.82085478874689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.65547198847848	80.49261896094225
2	6.030644032261712	10.592326276797575
3	1.3307979073486023	3.5061542932328944
4	0.4518633298470286	1.5873209549942215
5	0.20235276345385067	0.8885396327691133
6	0.10878669435435205	0.5732244292704805
7	0.06290570306485296	0.3867102829969738
8	0.03737096548258236	0.26255601063689105
9	0.02794251843240705	0.22085422683158726
>10	0.08916892017997276	1.2159914522367374
>50	0.002093600015288145	0.12621231746083122
>100	5.429286741560366E-4	0.09575385481819254
>500	2.9324203382171943E-5	0.020576460090101004
>1k	2.9324203360679007E-5	0.03116084692211988
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.452245349005607E-5	0.0	0.0	0.0
3	0.0	3.452245349005607E-5	0.0	0.0	0.0
4	0.0	6.904490698011213E-5	0.0	0.0	0.0
5	0.0	6.904490698011213E-5	0.0	1.7261226745028033E-5	0.0
6	1.035673604701682E-4	6.904490698011213E-5	0.0	1.7261226745028033E-5	1.7261226745028033E-5
7	1.035673604701682E-4	6.904490698011213E-5	0.0	1.7261226745028033E-5	1.7261226745028033E-5
8	1.035673604701682E-4	6.904490698011213E-5	0.0	1.7261226745028033E-5	3.452245349005607E-5
9	1.035673604701682E-4	1.553510407052523E-4	0.0	1.7261226745028033E-5	3.452245349005607E-5
10-11	1.035673604701682E-4	1.553510407052523E-4	0.0	4.3153066862570085E-5	3.452245349005607E-5
12-13	1.2082858721519623E-4	1.7261226745028034E-4	0.0	5.17836802350841E-5	3.452245349005607E-5
14-15	1.2082858721519623E-4	2.3302656105787843E-4	0.0	5.17836802350841E-5	5.17836802350841E-5
16-17	1.3808981396022427E-4	2.4165717443039246E-4	0.0	5.17836802350841E-5	6.904490698011213E-5
18-19	1.3808981396022427E-4	2.6754901454793454E-4	0.0	8.630613372514017E-5	6.904490698011213E-5
20-21	1.3808981396022427E-4	2.934408546654766E-4	0.0	8.630613372514017E-5	6.904490698011213E-5
22-23	1.3808981396022427E-4	3.624857616455887E-4	0.0	1.2945920058771024E-4	8.630613372514017E-5
24-25	1.3808981396022427E-4	4.229000552531868E-4	0.0	1.3808981396022427E-4	1.2945920058771024E-4
26-27	1.3808981396022427E-4	4.315306686257008E-4	0.0	2.157653343128504E-4	1.3808981396022427E-4
28-29	1.3808981396022427E-4	4.315306686257008E-4	0.0	6.645572296835793E-4	1.3808981396022427E-4
30-31	1.3808981396022427E-4	4.6605312211575687E-4	0.0	0.0021490227297559904	1.467204273327383E-4
32-33	1.3808981396022427E-4	4.6605312211575687E-4	0.0	0.004815882261862821	1.8987349419530836E-4
34-35	1.467204273327383E-4	5.609898692134111E-4	0.0	0.008328541904476027	2.2439594768536444E-4
36-37	1.553510407052523E-4	6.559266163110653E-4	0.0	0.014275034518138184	2.2439594768536444E-4
38-39	1.553510407052523E-4	6.559266163110653E-4	0.0	0.028351564928708543	2.2439594768536444E-4
40-41	1.553510407052523E-4	7.767552035262614E-4	0.0	0.04367090366492092	2.4165717443039246E-4
42-43	1.7261226745028034E-4	8.112776570163175E-4	0.0	0.05251728237174779	2.502877878029065E-4
44-45	1.7261226745028034E-4	9.234756308589998E-4	0.0	0.06346090012809556	2.6754901454793454E-4
46-47	1.7261226745028034E-4	0.001018412377956654	0.0	0.07487057100655908	2.8481024129296253E-4
48-49	1.7261226745028034E-4	0.001027042991329168	0.0	0.08579692753616183	3.107020814105046E-4
50-51	1.7261226745028034E-4	0.0011651328052893922	0.0	0.0964902575047067	3.107020814105046E-4
52-53	1.7261226745028034E-4	0.0011910246454069342	0.0	0.12399602232290888	3.3659392152804663E-4
54-55	1.8987349419530836E-4	0.0012082858721519623	0.0	0.15692181233904984	3.624857616455887E-4
56-57	2.071347209403364E-4	0.0012428083256420183	0.0	0.19974691589346438	3.624857616455887E-4
58-59	2.071347209403364E-4	0.0012428083256420183	0.0	0.2335012447933667	3.624857616455887E-4
60-61	2.071347209403364E-4	0.0012428083256420183	0.0	0.24491954628520274	3.624857616455887E-4
62-63	2.4165717443039246E-4	0.0012428083256420183	0.0	0.2586853746143626	3.624857616455887E-4
64-65	2.4165717443039246E-4	0.0013981593663472706	0.0	0.26490804685594527	3.624857616455887E-4
66-67	2.4165717443039246E-4	0.0014758348866998968	0.0	0.27018135162655127	3.7111637501810273E-4
68-69	2.589184011754205E-4	0.0015017267268174388	0.0	0.2756617911180977	3.797469883906167E-4
70-71	2.589184011754205E-4	0.001510357340189953	0.0	0.280184232525295	3.970082151356448E-4
72-73	2.589184011754205E-4	0.001518987953562467	0.0	0.28443912491794443	4.142694418806728E-4
74-75	2.8481024129296253E-4	0.001553510407052523	0.0	0.28756340695879457	4.142694418806728E-4
76-77	3.107020814105046E-4	0.001588032860542579	0.0	0.29102428292117266	4.229000552531868E-4
78-79	3.452245349005607E-4	0.001605294087287607	0.0	0.2931733056509286	4.315306686257008E-4
80-81	3.538551482730747E-4	0.001605294087287607	0.0	0.29388964656084726	4.487918953707289E-4
82-83	3.624857616455887E-4	0.001605294087287607	0.0	0.2943298078428455	4.6605312211575687E-4
84-85	3.624857616455887E-4	0.001605294087287607	0.0	0.2945628344039034	4.6605312211575687E-4
86-87	3.624857616455887E-4	0.001657077767522691	0.0	0.29466640176437353	4.6605312211575687E-4
88	3.624857616455887E-4	0.0017261226745028033	0.0	0.2946836629911186	4.6605312211575687E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8030	0.0	49.655254	1
GTATCAA	14990	0.0	37.94264	1
TCAACGC	17755	0.0	31.427767	4
ATCAACG	17930	0.0	31.073528	3
CAACGCA	18295	0.0	30.453062	5
TATCAAC	18365	0.0	30.433413	2
AACGCAG	18925	0.0	29.4393	6
TGGTATC	3810	0.0	27.3157	2
GTGGTAT	4160	0.0	26.767702	1
ACGCAGA	21815	0.0	25.578512	7
CGCAGAG	22455	0.0	24.849272	8
GCAGAGT	25570	0.0	21.855568	9
GTACATG	19035	0.0	20.407295	1
GAGTACT	14920	0.0	20.005358	12-13
TACATGG	19055	0.0	19.823973	2
ACATGGG	19130	0.0	19.20728	3
CAGAGTA	25260	0.0	18.936605	10-11
GTACTTT	16065	0.0	18.579594	14-15
AGAGTAC	23310	0.0	18.316193	10-11
AGTACTT	16115	0.0	16.688284	12-13
>>END_MODULE