FastQCFastQC Report
Fri 27 May 2016
SRR2139587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6909193
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108000.052.85341
GTATCAA201000.039.187461
CTTATAC57250.032.2773251
TCAACGC249750.030.9447844
TATCAAC251500.030.8491122
ATCAACG250650.030.7831783
CAACGCA255500.030.2483775
AACGCAG263750.029.3182136
GTGGTAT48450.027.0810531
TATACAC78150.026.7204483
TGGTATC48000.025.7528532
ACGCAGA302950.025.5524547
CGCAGAG310250.024.9642758
TTATACA83500.022.9386752
GCAGAGT356100.021.8447489
ACATCTC90550.021.2439128
ACACATC89500.020.6451766
GAGTACT196800.020.40656512-13
TATGCCG49850.020.11750442-43
TACACAT102650.019.8908775
Produced by FastQC (version 0.11.5)