##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6909193
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.065039144224222	33.0	31.0	33.0	25.0	34.0
2	32.18741508595866	33.0	33.0	33.0	29.0	34.0
3	32.21739123512688	33.0	33.0	33.0	31.0	34.0
4	32.85253038958385	33.0	33.0	34.0	31.0	34.0
5	32.9839600659585	33.0	33.0	34.0	31.0	34.0
6	36.64456659410151	38.0	37.0	38.0	34.0	38.0
7	37.135726704985665	38.0	38.0	38.0	36.0	38.0
8	37.0540980690509	38.0	38.0	38.0	36.0	38.0
9	37.21356227854686	38.0	38.0	38.0	36.0	38.0
10-11	37.337885625716346	38.0	38.0	38.0	37.0	38.0
12-13	37.33793765784225	38.0	38.0	38.0	37.0	38.0
14-15	37.4164810564707	38.0	38.0	38.0	37.0	38.0
16-17	37.43274699375166	38.0	38.0	38.0	37.0	38.0
18-19	37.44025177759544	38.0	38.0	38.0	37.0	38.0
20-21	37.451187401235636	38.0	38.0	38.0	37.0	38.0
22-23	37.46177396706055	38.0	38.0	38.0	37.0	38.0
24-25	37.475674788842824	38.0	38.0	38.0	37.0	38.0
26-27	37.44787649456075	38.0	38.0	38.0	37.0	38.0
28-29	37.42711900129075	38.0	38.0	38.0	37.0	38.0
30-31	37.431337885846276	38.0	38.0	38.0	37.0	38.0
32-33	37.42064736033673	38.0	38.0	38.0	37.0	38.0
34-35	37.392147326162416	38.0	38.0	38.0	37.0	38.0
36-37	37.37489217784365	38.0	38.0	38.0	37.0	38.0
38-39	37.35007615951846	38.0	38.0	38.0	37.0	38.0
40-41	37.35415273193388	38.0	38.0	38.0	37.0	38.0
42-43	37.32704962645941	38.0	38.0	38.0	37.0	38.0
44-45	37.286055515322516	38.0	38.0	38.0	37.0	38.0
46-47	37.241762606924354	38.0	38.0	38.0	37.0	38.0
48-49	37.18191921339801	38.0	38.0	38.0	37.0	38.0
50-51	37.10912435429067	38.0	38.0	38.0	37.0	38.0
52-53	37.13372024662759	38.0	38.0	38.0	37.0	38.0
54-55	37.11493484758769	38.0	38.0	38.0	36.0	38.0
56-57	37.06876219441865	38.0	38.0	38.0	36.0	38.0
58-59	37.057173079748175	38.0	38.0	38.0	36.0	38.0
60-61	37.07555746441172	38.0	38.0	38.0	36.0	38.0
62-63	37.076391164379004	38.0	38.0	38.0	36.0	38.0
64-65	37.02355209654829	38.0	38.0	38.0	36.0	38.0
66-67	37.06443679045442	38.0	38.0	38.0	36.0	38.0
68-69	37.07468999566724	38.0	38.0	38.0	36.0	38.0
70-71	37.057365729040015	38.0	38.0	38.0	36.0	38.0
72-73	37.04869239290223	38.0	38.0	38.0	36.0	38.0
74-75	37.008805072872285	38.0	38.0	38.0	36.0	38.0
76-77	37.01379772689447	38.0	38.0	38.0	36.0	38.0
78-79	37.0165752319335	38.0	38.0	38.0	36.0	38.0
80-81	37.013887283778274	38.0	38.0	38.0	36.0	38.0
82-83	36.96354784154665	38.0	38.0	38.0	36.0	38.0
84-85	36.95104525917277	38.0	38.0	38.0	36.0	38.0
86-87	36.91972317834499	38.0	38.0	38.0	35.5	38.0
88-89	36.929261477557304	38.0	38.0	38.0	35.0	38.0
90-91	36.917710329639036	38.0	38.0	38.0	35.0	38.0
92-93	36.898522844006685	38.0	38.0	38.0	35.0	38.0
94-95	36.88576865415793	38.0	38.0	38.0	35.0	38.0
96-97	36.86652172681156	38.0	38.0	38.0	35.0	38.0
98-99	36.90354969119255	38.0	38.0	38.0	35.0	38.0
100	35.46197866777007	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	16.0
21	77.0
22	317.0
23	1022.0
24	2537.0
25	5522.0
26	9895.0
27	16698.0
28	26733.0
29	39512.0
30	54242.0
31	70745.0
32	91297.0
33	120129.0
34	175494.0
35	304667.0
36	828251.0
37	5162036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76539865075415	19.57031740175734	14.18631669429411	24.477967253194404
2	14.657171872032757	19.996401673930865	42.64779190732704	22.69863454670934
3	18.443535917583585	25.293000877537175	29.974950460327083	26.288512744552158
4	12.651671355596076	17.546042353494233	38.211498843133676	31.59078744777602
5	13.764849237819815	36.83599517338711	35.08573287792077	14.313422710872311
6	30.68485711717707	36.855346203239655	18.35278012931467	14.107016550268606
7	27.264910219612087	32.49226350555793	22.06609884852479	18.176727426305195
8	24.780260154840082	36.3869991763148	20.55099633198841	18.281744336856708
9	25.751791851812506	17.029687837638928	20.92389082198167	36.2946294885669
10-11	24.48162904119193	26.639905702446004	28.14656501851953	20.731900237842538
12-13	25.422932895346822	24.146568202683007	28.446513217969162	21.983985684001013
14-15	22.50647796349009	25.408922576051935	26.367435386448175	25.7171640740098
16-17	21.901906633669086	28.726972889597963	27.04517734560317	22.325943131129787
18-19	21.91645247136677	27.53530694539869	29.112206013061147	21.43603457017339
20-21	22.81388404823797	26.534176764489626	29.091774881749817	21.56016430552259
22-23	22.49946824028461	26.592118260755278	28.941978736703927	21.966434762256185
24-25	22.247532479150763	26.746220748063816	29.109913409680043	21.896333363105374
26-27	22.108235277085058	26.880388870992846	29.121558379511157	21.889817472410936
28-29	21.969822504062968	26.74231802103763	29.27135991378686	22.016499561112543
30-31	22.335518866621364	26.77892574613129	29.022263115719515	21.863292271527826
32-33	22.06687996748299	26.78891171081957	29.059236696307718	22.084971625389727
34-35	22.1056000494691	26.891589676854622	29.22715249834635	21.775657775329925
36-37	22.181403447343804	26.79165992146016	28.991031866430994	22.035904764765043
38-39	21.958152354701575	26.79827823847375	29.19539999334846	22.048169413476217
40-41	22.184416961038874	26.689394392399794	29.080745327773595	22.04544331878774
42-43	22.195468614436397	26.73026624200438	28.898161488647588	22.176103654911632
44-45	22.139095838929823	26.93581640674681	28.774873788884666	22.150213965438702
46-47	22.330116705076293	26.848953790108983	28.386487660323077	22.434441844491644
48-49	22.270777171987767	26.905575300795782	28.359391735509686	22.464255791706762
50-51	22.174003977713607	27.112892115014702	28.300336037494382	22.412767869777305
52-53	22.3367160006957	27.100366057021212	28.31215265084345	22.250765291439638
54-55	22.30529188471168	27.06388723785661	28.17293801886408	22.457882858567633
56-57	22.371612201898962	27.259150318158916	27.98952745700388	22.379710022938237
58-59	22.1892817854025	27.335774351950842	28.237229850831003	22.237714011815658
60-61	22.380102350986636	27.465871475143643	27.983827821427298	22.170198352442423
62-63	22.177349044594465	27.641953164032657	27.988071917072112	22.192625874300766
64-65	22.203335884398367	27.563258660998446	27.951149881993555	22.282255572609635
66-67	22.268984863447987	27.540203900605242	27.863860841171263	22.326950394775512
68-69	22.238632721586857	27.43594885515798	27.996752466382834	22.328665956872324
70-71	22.270431059766718	27.138913039508434	28.102787818348435	22.487868082376412
72-73	22.360875252278195	27.082787166282714	28.064321491520495	22.4920160899186
74-75	22.270288275061084	27.235693076358373	27.979120602189184	22.51489804639136
76-77	22.381362016819583	27.14660688007565	27.959667434725482	22.512363668379283
78-79	22.359380821720077	27.116078421751023	27.89603724790511	22.62850350862379
80-81	22.36781643338246	27.22234609065491	27.87231726483045	22.537520211132183
82-83	22.48286652698901	27.201530604300356	27.87802135979991	22.437581508910725
84-85	22.530579162663685	27.20427294338363	27.77986056888362	22.48528732506907
86-87	22.39787919765123	27.242436023488114	27.859790693657278	22.49989408520338
88-89	22.479213594672736	27.21124007968315	27.79994508849916	22.509601237144956
90-91	22.516129266870298	27.2682460387623	27.763028728842087	22.452595965525312
92-93	22.536116110761302	27.271859231370684	27.740333932516638	22.45169072535138
94-95	22.39773708768148	27.31740915005722	27.758343406099016	22.526510356162277
96-97	22.58177868483808	27.409934795102714	27.754968947225283	22.25331757283393
98-99	23.615489605837382	29.30495889095588	27.991047569729723	19.088503933477014
100	22.489799154767578	30.201701948482203	21.883353551224786	25.425145345525436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2122.0
1	1809.0
2	1850.0
3	2386.0
4	2900.5
5	3907.0
6	5084.0
7	7015.0
8	9583.0
9	10962.0
10	10700.5
11	10324.5
12	11330.0
13	14103.0
14	19486.0
15	25794.5
16	30428.5
17	32703.0
18	32217.5
19	29633.0
20	26405.5
21	23791.5
22	23386.0
23	26103.5
24	31542.5
25	39851.5
26	50793.5
27	63044.0
28	75734.0
29	90048.0
30	104490.5
31	118600.0
32	134659.0
33	150696.0
34	166920.0
35	183474.0
36	200511.0
37	215606.0
38	226673.0
39	237293.5
40	248029.0
41	257223.0
42	264943.5
43	277601.0
44	290595.0
45	296647.5
46	297104.0
47	294341.0
48	292586.5
49	291928.5
50	289825.5
51	284468.5
52	276141.5
53	264434.0
54	248959.5
55	232159.5
56	214669.0
57	195388.5
58	174942.0
59	153497.0
60	130284.0
61	107146.5
62	86347.0
63	67706.0
64	52314.0
65	39722.5
66	29410.0
67	22394.5
68	17285.0
69	12993.5
70	9659.5
71	7005.5
72	5011.0
73	3504.0
74	2375.5
75	1568.5
76	1044.5
77	657.5
78	423.0
79	287.0
80	193.5
81	141.0
82	109.5
83	73.5
84	40.0
85	25.0
86	16.5
87	12.5
88	10.5
89	7.5
90	6.0
91	3.5
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006151224897032114
3	0.0012157715090604648
4	1.5920817380553706E-4
5	0.0
6	0.0
7	8.105143393736433E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.4473704968866339E-5
22-23	7.743837895274073E-4
24-25	6.079829023627228E-4
26-27	7.96272806248512E-5
28-29	0.0029258307693689274
30-31	0.0021028013156575236
32-33	0.004688056365991691
34-35	0.010003658043178033
36-37	0.001574874092478804
38-39	0.0
40-41	1.0399427912614201E-4
42-43	7.108416065319609E-4
44-45	2.1888999524782274E-4
46-47	6.8624880163803E-5
48-49	0.00450592772122218
50-51	0.003638589197681752
52-53	4.077564690171736E-4
54-55	0.0032742753708294805
56-57	0.04367241680169115
58-59	0.06598424920191132
60-61	0.031997839149230094
62-63	0.011480944055527693
64-65	0.0010529146834009906
66-67	1.7593207346420725E-4
68-69	0.0016266208705115748
70-71	0.01184695076889025
72-73	0.018090205312117212
74-75	0.011735614056583715
76-77	0.013930824291212856
78-79	0.003295088518344644
80-81	0.01532976423583208
82-83	0.04685682100914102
84-85	0.025896817235826097
86-87	0.004002624150319111
88-89	9.457707355268468E-5
90-91	9.569708580363399E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	66.0
20-21	265.0
22-23	666.0
24-25	696.0
26-27	2300.0
28-29	5927.0
30-31	15546.0
32-33	20114.0
34-35	27599.0
36-37	43119.0
38-39	49763.0
40-41	48089.0
42-43	54769.0
44-45	65845.0
46-47	67044.0
48-49	62204.0
50-51	55799.0
52-53	53156.0
54-55	57886.0
56-57	59048.0
58-59	58911.0
60-61	58098.0
62-63	56650.0
64-65	61340.0
66-67	65596.0
68-69	67016.0
70-71	73982.0
72-73	58592.0
74-75	58250.0
76-77	61192.0
78-79	62305.0
80-81	61402.0
82-83	57591.0
84-85	57252.0
86-87	59262.0
88-89	61299.0
90-91	62174.0
92-93	61091.0
94-95	69853.0
96-97	208780.0
98-99	449804.0
100-101	4388852.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.34158096611424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.65114965947042	80.96607458279559
2	6.189178536183791	10.935236335360335
3	1.2591379801613007	3.337027194657872
4	0.4347567295614872	1.5362838730047659
5	0.18885823927311515	0.8342017717931838
6	0.10114122086000647	0.5360985210969542
7	0.04944160489513309	0.3057424679356621
8	0.032214360117398026	0.22766940020661433
9	0.02308964766941099	0.18357983811596892
>10	0.06923621185883455	0.9603413037812185
>50	0.0014255351925651983	0.0822608301412449
>100	2.9970974006285936E-4	0.042995074366344026
>500	4.704334710173632E-5	0.030670421362593563
>1k	2.3521669435726397E-5	0.021818385381601177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4473470345957915E-5	0.0
2	0.0	0.0	0.0	1.4473470345957915E-5	0.0
3	0.0	0.0	0.0	4.342041103787374E-5	0.0
4	0.0	4.342041103787374E-5	0.0	5.789388138383166E-5	0.0
5	0.0	4.342041103787374E-5	0.0	8.684082207574748E-5	0.0
6	0.0	4.342041103787374E-5	0.0	8.684082207574748E-5	0.0
7	0.0	8.684082207574748E-5	0.0	8.684082207574748E-5	0.0
8	0.0	1.0131429242170541E-4	0.0	8.684082207574748E-5	0.0
9	0.0	1.7368164415149497E-4	0.0	1.1578776276766332E-4	0.0
10-11	2.894694069191583E-5	1.7368164415149497E-4	0.0	1.374979682866002E-4	0.0
12-13	4.342041103787374E-5	1.7368164415149497E-4	0.0	1.5920817380553706E-4	0.0
14-15	5.06571462108527E-5	2.243387903623477E-4	0.0	1.66444908978516E-4	0.0
16-17	5.789388138383166E-5	2.605224662272425E-4	0.0	1.7368164415149497E-4	0.0
18-19	5.789388138383166E-5	2.749959365732004E-4	0.0	1.8091837932447392E-4	7.236735172978957E-6
20-21	6.513061655681062E-5	2.894694069191583E-4	0.0	2.0262858484341081E-4	2.171020551893687E-5
22-23	7.236735172978957E-5	3.184163476110741E-4	0.0	2.1710205518936872E-4	3.618367586489479E-5
24-25	7.960408690276853E-5	3.40126553130011E-4	0.0	2.532857310542635E-4	4.342041103787374E-5
26-27	8.684082207574748E-5	3.4736328830298994E-4	0.0	3.039428772651162E-4	4.342041103787374E-5
28-29	8.684082207574748E-5	3.6183675864894784E-4	0.0	6.513061655681061E-4	5.06571462108527E-5
30-31	9.407755724872644E-5	4.3420411037873745E-4	0.0	0.0018381307339366553	5.789388138383166E-5
32-33	1.0131429242170541E-4	4.3420411037873745E-4	0.0	0.004001914550657363	5.789388138383166E-5
34-35	1.0855102759468436E-4	4.920979917625691E-4	0.0	0.006874898414330009	5.789388138383166E-5
36-37	1.1578776276766332E-4	5.427551379734218E-4	0.0	0.011007074198100994	5.789388138383166E-5
38-39	1.1578776276766332E-4	5.499918731464008E-4	0.0	0.017889209347603983	6.513061655681062E-5
40-41	1.1578776276766332E-4	5.934122841842745E-4	0.0	0.024460164884668876	7.236735172978957E-5
42-43	1.1578776276766332E-4	6.151224897032114E-4	0.0	0.031197565330712284	7.960408690276853E-5
44-45	1.1578776276766332E-4	7.164367821249167E-4	0.0	0.03862969235336167	8.684082207574748E-5
46-47	1.1578776276766332E-4	7.960408690276852E-4	0.0	0.04620655407947064	1.0131429242170541E-4
48-49	1.1578776276766332E-4	8.105143393736431E-4	0.0	0.05401499133111494	1.0131429242170541E-4
50-51	1.1578776276766332E-4	9.190653669683276E-4	0.0	0.06146159182411028	1.0131429242170541E-4
52-53	1.1578776276766332E-4	9.263021021413065E-4	0.0	0.06888648211158668	1.0131429242170541E-4
54-55	1.1578776276766332E-4	9.263021021413065E-4	0.0	0.07626071525285225	1.0131429242170541E-4
56-57	1.1578776276766332E-4	9.407755724872644E-4	0.0	0.08343955654444737	1.0131429242170541E-4
58-59	1.1578776276766332E-4	9.697225131791803E-4	0.0	0.09091510397813463	1.0131429242170541E-4
60-61	1.4473470345957915E-4	9.697225131791803E-4	0.0	0.09803605138834592	1.0131429242170541E-4
62-63	1.4473470345957915E-4	9.697225131791803E-4	0.0	0.10458529671989189	1.1578776276766332E-4
64-65	1.4473470345957915E-4	0.0011578776276766332	0.0	0.11127204001972443	1.1578776276766332E-4
66-67	1.4473470345957915E-4	0.0011578776276766332	0.0	0.11801667720094083	1.1578776276766332E-4
68-69	1.4473470345957915E-4	0.001165114362849612	0.0	0.1244284245642002	1.2302449794064228E-4
70-71	1.519714386325581E-4	0.00117958783319557	0.0	0.13007307799912377	1.3026123311362124E-4
72-73	1.66444908978516E-4	0.001186824568368549	0.0	0.13438617216221924	1.3026123311362124E-4
74-75	1.7368164415149497E-4	0.001194061303541528	0.0	0.13761375604936785	1.4473470345957915E-4
76-77	1.7368164415149497E-4	0.0012302449794064228	0.0	0.1407906827903056	1.4473470345957915E-4
78-79	1.7368164415149497E-4	0.0012447184497523806	0.0	0.1428531523146046	1.519714386325581E-4
80-81	1.7368164415149497E-4	0.0012447184497523806	0.0	0.14367090338915123	1.5920817380553706E-4
82-83	1.7368164415149497E-4	0.0012447184497523806	0.0	0.14419194832160573	1.5920817380553706E-4
84-85	1.7368164415149497E-4	0.0012447184497523806	0.0	0.1445031279340438	1.5920817380553706E-4
86-87	1.7368164415149497E-4	0.0012591919200983384	0.0	0.14466233610784934	1.5920817380553706E-4
88	1.7368164415149497E-4	0.0013026123311362123	0.0	0.14467680957819531	1.5920817380553706E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10800	0.0	52.8534	1
GTATCAA	20100	0.0	39.18746	1
CTTATAC	5725	0.0	32.277325	1
TCAACGC	24975	0.0	30.944784	4
TATCAAC	25150	0.0	30.849112	2
ATCAACG	25065	0.0	30.783178	3
CAACGCA	25550	0.0	30.248377	5
AACGCAG	26375	0.0	29.318213	6
GTGGTAT	4845	0.0	27.081053	1
TATACAC	7815	0.0	26.720448	3
TGGTATC	4800	0.0	25.752853	2
ACGCAGA	30295	0.0	25.552454	7
CGCAGAG	31025	0.0	24.964275	8
TTATACA	8350	0.0	22.938675	2
GCAGAGT	35610	0.0	21.844748	9
ACATCTC	9055	0.0	21.243912	8
ACACATC	8950	0.0	20.645176	6
GAGTACT	19680	0.0	20.406565	12-13
TATGCCG	4985	0.0	20.117504	42-43
TACACAT	10265	0.0	19.890877	5
>>END_MODULE